On the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring

Biomanufacturers are being incited by regulatory agencies to transition from a quality by testing framework, where they extensively test their product after their production, to more of a quality by design or even quality by control framework. This requires powerful analytical tools and sensors enabling measurements of key process variables and/or product quality attributes during production, preferably in an online manner. As such, the demand for monitoring technologies is rapidly growing. In this context, we believe surface plasmon resonance (SPR)-based biosensors can play a role in enabling the development of improved bioprocess monitoring and control strategies. The SPR technique has been profusely used to probe the binding behavior of a solution species with a sensor surface-immobilized partner in an investigative context, but its ability to detect binding in real-time and without a label has been exploited for monitoring purposes and is promising for the near future. In this review, we examine applications of SPR that are or could be related to bioprocess monitoring in three spheres: biotherapeutics production monitoring, vaccine monitoring, and bacteria and contaminant detection. These applications mainly exploit SPR’s ability to measure solution species concentrations, but performing kinetic analyses is also possible and could prove useful for product quality assessments. We follow with a discussion on the limitations of SPR in a monitoring role and how recent advances in hardware and SPR response modeling could counter them. Mainly, throughput limitations can be addressed by multi-detection spot instruments, and nonspecific binding effects can be alleviated by new antifouling materials. A plethora of methods are available for cell growth and metabolism monitoring, but product monitoring is performed mainly a posteriori. SPR-based biosensors exhibit potential as product monitoring tools from early production to the end of downstream processing, paving the way for more efficient production control. However, more work needs to be done to facilitate or eliminate the need for sample preprocessing and to optimize the experimental protocols.

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High-Affinity Interaction between the S-Layer Protein SbsC and the Secondary Cell Wall Polymer of Geobacillus stearothermophilus ATCC 12980 Determined by Surface Plasmon Resonance Technology

Journal of Bacteriology ◽

10.1128/jb.00294-07 ◽

2007 ◽

Vol 189 (19) ◽

pp. 7154-7158 ◽

Cited By ~ 19

Author(s):

Judith Ferner-Ortner ◽

Christoph Mader ◽

Nicola Ilk ◽

Uwe B. Sleytr ◽

Eva M. Egelseer

Keyword(s):

Amino Acids ◽

Cell Wall ◽

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Secondary Cell Wall ◽

Geobacillus Stearothermophilus ◽

Binding Behavior ◽

Cell Wall Polymer ◽

Secondary Cell Wall Polymer

ABSTRACT Surface plasmon resonance studies using C-terminal truncation forms of the S-layer protein SbsC (recombinant SbsC consisting of amino acids 31 to 270 [rSbsC31-270] and rSbsC31-443) and the secondary cell wall polymer (SCWP) isolated from Geobacillus stearothermophilus ATCC 12980 confirmed the exclusive responsibility of the N-terminal region comprising amino acids 31 to 270 for SCWP binding. Quantitative analyses indicated binding behavior demonstrating low, medium, and high affinities.

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Dynamics of Dental Pellicle Formation - In Vitro Analysis of Time Dependant Binding Behavior by Surface Plasmon Resonance and the Influence of Oral Therapeutics

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.415.77 ◽

2009 ◽

Vol 415 ◽

pp. 77-80

Author(s):

Doru Vornicescu ◽

Katerina Solanska ◽

Ioana Demetrescu ◽

Matthias Frentzen ◽

Michael Keusgen

Keyword(s):

Surface Plasmon Resonance ◽

Real Time ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Artificial Saliva ◽

Human Saliva ◽

Forming Process ◽

Pellicle Formation ◽

Binding Behavior

: The pellicle on oral surfaces represents a central interface for the formation of biofilms. Among other things it causes the first adsorption of bacteria. The dynamics of pellicle formation, on tooth surfaces and the influence of oral therapeutics on the pellicle structure are fairly unknown. With the method of surface plasmon resonance (SPR), the formation of salivary pellicle structures on hydroxylapatite (HAP) surfaces covering a very thin (~50nm) layer gold on a glass prism was recorded in real time without labeling or destruction. As pellicle forming substrates natural pooled human saliva (NS) and artificial saliva (AS) were used. To simulate the influence of therapeutic additives on the dynamic of the pellicle forming process, a chlorhexidine preparate (Chlorhexamed Fluid® CHX) on two different concentrations was selected. The binding behavior of a NS and a preparation in terms of an AS were compared. The layer was largely stable against rinsing with buffer. The application of CHX preparations in two different concentrations as an example of an oral therapeutic additive revealed a complex dynamic of adsorption. CHX did not lead to any visible destruction of the pellicle. The introduced method is an excellent tool to illustrate the dynamic effects of pellicle formation or pellicle reorganization by measuring the increase or decrease of the SPR signal in real time.

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Multiplexed, High-Sensitivity Measurements of Antibody Affinity Using Interferometric Reflectance Imaging Sensor

Biosensors ◽

10.3390/bios11120483 ◽

2021 ◽

Vol 11 (12) ◽

pp. 483

Author(s):

Allison Marn ◽

James Needham ◽

Elisa Chiodi ◽

M. Ünlü

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Imaging Modality ◽

Lethal Factor ◽

Antibody Affinity ◽

Binding Behavior ◽

Anthrax Lethal Factor ◽

Imaging Sensor ◽

Complementary Binding

Anthrax lethal factor (LF) is one of the enzymatic components of the anthrax toxin responsible for the pathogenic responses of the anthrax disease. The ability to screen multiplexed ligands against LF and subsequently estimate the effective kinetic rates (kon and koff) and complementary binding behavior provides critical information useful in diagnostic and therapeutic development for anthrax. Tools such as biolayer interferometry (BLI) and surface plasmon resonance imaging (SPRi) have been developed for this purpose; however, these tools suffer from limitations such as signal jumps when the solution in the chamber is switched or low sensitivity. Here, we present multiplexed antibody affinity measurements obtained by the interferometric reflectance imaging sensor (IRIS), a highly sensitive, label-free optical biosensor, whose stability, simplicity, and imaging modality overcomes many of the limitations of other multiplexed methods. We compare the multiplexed binding results obtained with the IRIS system using two ligands targeting the anthrax lethal factor (LF) against previously published results obtained with more traditional surface plasmon resonance (SPR), which showed consistent results, as well as kinetic information previously unattainable with SPR. Additional exemplary data demonstrating multiplexed binding and the corresponding complementary binding to sequentially injected ligands provides an additional layer of information immediately useful to the researcher.

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The geometric determinants of programmed antibody migration and binding on multi-antigen substrates

10.1101/2020.10.12.336164 ◽

2020 ◽

Author(s):

Ian T. Hoffecker ◽

Alan Shaw ◽

Viktoria Sorokina ◽

Ioanna Smyrlaki ◽

Björn Högberg

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Bound States ◽

De Novo ◽

Affinity Maturation ◽

Host Immune System ◽

Patterned Surface ◽

Binding Behavior ◽

Parameterized Model

AbstractViruses and bacteria commonly exhibit spatial repetition of surface molecules that directly interface with the host immune system. However the complex interaction of patterned surfaces with multivalent immune molecules such as immunoglobulins and B-cell receptors is poorly understood, and standard characterization typically emphasizes the monovalent affinity. We developed a mechanistic model of multivalent antibody-antigen interactions as well as a pipeline for constructing such models from a minimal dataset of patterned surface plasmon resonance experiments in which antigen pattern geometries are precisely defined using DNA origami nanostructures. We modeled the change in binding enhancement due to multivalence and spatial tolerance,i.e. the strain-dependent interconversion of bound antibodies from monovalently bound to bivalently bound states at varying antigen separation distances. The parameterized model enables mechanistic post hoc characterization of binding behavior in patterned surface plasmon resonance experiments as well as de novo simulation of transient dynamics and equilibrium properties of arbitrary pattern geometries. Simulation on lattices shows that antigen spacing is a spatial control parameter that can be tuned to determine antibody residence time and migration speed. We found that gradients in antigen spacing are predicted to drive persistent, directed antibody migration toward favorable spacing. These results indicate that antigen pattern geometry can influence antibody interactions, a phenomenon that could be significant during the coevolution of pathogens and immunity in processes like pathogen neutralization or affinity maturation.

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Handbook of Surface Plasmon Resonance

10.1039/9781788010283 ◽

2017 ◽

Cited By ~ 46

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance

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Comparison of sensitivity of the refractometric methods of frustrated total internal reflection and surface plasmon resonance

Izmeritel`naya Tekhnika ◽

10.32446/0369-1025it.2020-2-44-49 ◽

2020 ◽

pp. 44-49

Author(s):

I. N. Pavlov

Keyword(s):

Surface Plasmon Resonance ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Total Internal Reflection ◽

Resonance Method ◽

Internal Reflection ◽

Optical Methods ◽

Thin Boundary Layer ◽

Experimental Conditions ◽

Frustrated Total Internal Reflection

Two optical methods, namely surface plasmon resonance imaging and frustrated total internal reflection, are described in the paper in terms of comparing their sensitivity to change of refractive index of a thin boundary layer of an investigated medium. It is shown that, despite the fact that the theoretically calculated sensitivity is higher for the frustrated total internal reflection method, and the fact that usually in practice the surface plasmon resonance method, on the contrary, is considered more sensitive, under the same experimental conditions both methods show a similar result.

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