scholarly journals Expression of Wilm’s Tumor Gene (WT1) in Endometrium with Potential Link to Gestational Vascular Transformation

2020 ◽  
Vol 1 (1) ◽  
pp. 17-31 ◽  
Author(s):  
Peilin Zhang
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Stromal Cells ◽  
Muscle Cells ◽  
Cellular Functions ◽  
Endometrial Stromal Cells ◽  
Wilm's Tumor ◽  
Wilm’S Tumor

Background: Wilm’s tumor 1 gene (WT1) is a transcription factor with versatile cellular functions in embryonic development, the maintenance of adult tissue functions, and regeneration. WT1 is known to be regulated by progesterone and it is abundantly expressed in endometrium, but its function is unclear. Design: in this observational and descriptive study, WT1 expression was detected by immunohistochemical staining in endometrium of various physiological and pathological conditions. Result: WT1 was detected in endometrial stromal cells and vascular smooth muscle cells, in both proliferative and secretory phases of menstrual cycles. WT1 appeared increased in vascular smooth muscle cells in spiral artery in early pregnancy and it was also detected in regenerative endothelial cells and smooth muscle cells in decidual vasculopathy at term. WT1 expression appeared decreased in endometrial stromal cells in adenomyosis (endometriosis). Conclusion: WT1 potentially links the hormonal effects on endometrial decidualization and may play a role in gestational vascular transformation during pregnancy and restoration after pregnancy.

scholarly journals Expression of Wilm’s Tumor Gene in Endometrium with Potential Link to Gestational Vascular Transformation

2020 ◽  
Author(s):  
Peilin Zhang
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Stromal Cells ◽  
Muscle Cells ◽  
Cellular Functions ◽  
Endometrial Stromal Cells ◽  
Potential Link ◽  
Tumor Gene

AbstractBackgroundWT1 is a transcription factor with versatile cellular functions in embryonic development, maintenance of adult tissue functions and regenerations. WT1 is known to be regulated by progesterone and it is abundantly expressed in endometrium, but its function is unclear.DesignWT1 expression was detected by immunohistochemical staining in endometrium of various physiological and pathological conditions.ResultWT1 was detected in endometrial stromal cells and vascular smooth muscle cells in both proliferative and secretory phases of menstrual cycles. WT1 appeared increased in vascular smooth muscle cells in spiral artery in early pregnancy and WT1 was also detected in regenerative endothelial cells and smooth muscle cells in decidual vasculopathy at term. WT1 expression was decreased in endometrial stromal cells in adenomyosis (endometriosis).ConclusionWT1 potentially links the hormonal (progesterone) effects on endometrial decidualization and may play a role in gestational vascular transformation during pregnancy and restoration after pregnancy.

scholarly journals A fibroblast-associated antigen: characterization in fibroblasts and immunoreactivity in smooth muscle differentiated stromal cells.

1992 ◽  
Vol 40 (4) ◽  
pp. 475-486 ◽  
Author(s):  
L Rønnov-Jessen ◽  
J E Celis ◽  
B Van Deurs ◽  
O W Petersen
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Stromal Cells ◽  
Breast Tissue ◽  
Muscle Cells ◽  
Human Breast ◽  
Human Breast Tissue ◽  
Coated Pits

Fibroblasts with smooth muscle differentiation are frequently derived from human breast tissue. Immunofluorescence cytochemistry of a fibroblast-associated antigen recognized by a monoclonal antibody (MAb), 1B10, was analyzed with a view to discriminating smooth muscle differentiated fibroblasts from vascular smooth muscle cells. The antigen was detected on the cell surface and in cathepsin D-positive and acridine orange-accumulating vesicular compartments of fibroblasts. Ultrastructurally, the antigen was revealed in coated pits and in endosomal and lysosomal structures. 1B10 recognized three major brands migrating at apparent Mr of 38,000, 45,000, and 80,000, in addition to many minor bands between Mr 45,000 and 97,000, including Mr 52,000. The Mr 45,000 and 38,000 were associated with the cell membrane and Mr 52,000 as well as Mr 38,000 were associated with the lysosomes. The 1B10 immunoreactivity was specific to fibroblasts and smooth muscle differentiated fibroblasts within the context of vascular smooth muscle cells.

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