scholarly journals Performance Improvement of the One-Dot Lateral Flow Immunoassay for Aflatoxin B1 by Using a Smartphone-Based Reading System

Sensors ◽  
2013 ◽  
Vol 13 (4) ◽  
pp. 5109-5116 ◽  
Author(s):  
Sangdae Lee ◽  
Giyoung Kim ◽  
Jihea Moon
Keyword(s):  
Performance Improvement ◽  
Aflatoxin B1 ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Reading System ◽  
The One

scholarly journals A multiplex chemiluminescent biosensor for type B-fumonisins and aflatoxin B1 quantitative detection in maize flour

The Analyst ◽  
2015 ◽  
Vol 140 (1) ◽  
pp. 358-365 ◽  
Author(s):  
Martina Zangheri ◽  
Fabio Di Nardo ◽  
Laura Anfossi ◽  
Cristina Giovannoli ◽  
Claudio Baggiani ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Type B ◽  
Maize Flour

A multiplex chemiluminescence biosensor based on a lateral flow immunoassay was developed for on-site quantitative detection of fumonisins and aflatoxin B1 in maize.

Development of a Smartphone-Based Reading System for Lateral Flow Immunoassay

2014 ◽  
Vol 14 (11) ◽  
pp. 8453-8457 ◽  
Author(s):  
Sangdae Lee ◽  
Giyoung Kim ◽  
Jihea Moon
Keyword(s):  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Reading System

scholarly journals Immunochromatographic Tests for Mycotoxins Detection with the Use of Ultrasmall Magnetite Nanoparticles

2021 ◽  
Vol 2 (1) ◽  
pp. 100
Author(s):  
Alina V. Petrakova ◽  
Alexandr E. Urusov ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Aflatoxin B1 ◽  
Magnetite Nanoparticles ◽  
Fe3o4 Nanoparticles ◽  
Raw Materials ◽  
Total Surface Area ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Control Food ◽  
Lateral Flow Tests ◽  
T2 Toxin

The use of small (with a diameter of 7–12 nm) superparamagnetic Fe3O4 nanoparticles as carriers for antibodies in lateral flow immunoassay is considered. Increased total surface area for such suspension provides a concentration of analytes with an increase in their concentration up to 50 times. When the concentrated complexes were redissolved, aggregates with diameters of 100–500 nm were obtained, serving as colored markers in the assay. Further, magnetite–antibodies complexes are more tolerant to methanol (up to 30%) than native antibodies, thus providing minimal dilution of tested extracts. Lateral flow tests for mycotoxins zearalenone, T2-toxin, and aflatoxin B1 were developed and demonstrated applicability to control food products and raw materials.

scholarly journals A Rapid and Sensitive Fluorescent Microsphere-Based Lateral Flow Immunoassay for Determination of Aflatoxin B1 in Distillers’ Grains

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2109
Author(s):  
Zifei Wang ◽  
Pengjie Luo ◽  
Baodong Zheng
Keyword(s):  
Aflatoxin B1 ◽  
High Performance ◽  
Animal Feed ◽  
Limit Of Detection ◽  
Lateral Flow ◽  
Distillers Grains ◽  
Detection Methods ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Fluorescent Microsphere

Aflatoxin B1 (AFB1) is a toxic compound naturally produced by the genera Aspergillus. Distillers’ grains can be used as animal feed since they have high content of crude protein and other nutrients. However, they are easily contaminated by mycotoxins, and currently there are no rapid detection methods for AFB1 in distillers’ grains. In this study, a lateral flow immunoassay (LFIA) based on red fluorescent microsphere (FM), is developed for quantitative detection of AFB1 in distillers’ grains. The whole test can be completed within 15 min, with the cut-off value being 25.0 μg/kg, and the quantitative limit of detection (qLOD) being 3.4 μg/kg. This method represents satisfactory recoveries of 95.2–113.0%, and the coefficients of variation (CVs) are less than 7.0%. Furthermore, this technique is successfully used to analyze AFB1 in real samples, and the results indicates good consistency with that of high-performance liquid chromatography (HPLC). The correlation coefficient is found to be greater than 0.99. The proposed test strip facilitates on-site, cost-effective, and sensitive monitoring of AFB1 in distillers’ grains.

Amino-functionalized CdSe/ZnS quantum dot-based lateral flow immunoassay for sensitive detection of aflatoxin B1

2018 ◽  
Vol 10 (29) ◽  
pp. 3582-3588 ◽  
Author(s):  
Jieying Li ◽  
Mao Mao ◽  
Feng Wu ◽  
Qiang Li ◽  
Luyao Wei ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Fluorescent Probes ◽  
Aflatoxin B1 ◽  
Lateral Flow ◽  
Sensitive Detection ◽  
Lateral Flow Immunoassay

In this study, highly fluorescent amino-functionalized CdSe/ZnS QDs coated with amphiphilic N-alkylated poly(ethyleneimine) have been synthesized and applied as fluorescent probes in LFIA strips for on-site determination of AFB1.

Colour-encoded lateral flow immunoassay for the simultaneous detection of aflatoxin B1 and type-B fumonisins in a single Test line

Talanta ◽  
2019 ◽  
Vol 192 ◽  
pp. 288-294 ◽  
Author(s):  
Fabio Di Nardo ◽  
Eugenio Alladio ◽  
Claudio Baggiani ◽  
Simone Cavalera ◽  
Cristina Giovannoli ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Test Line ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Type B ◽  
Single Test

scholarly journals EFFICIENT CONJUGATION OF AFLATOXIN B1 WITH BOVINE SERUM ALBUMIN APPLYING FOR DEVELOPMENT OF AFLATOXIN B1 QUICK TEST

2018 ◽  
Vol 56 (4A) ◽  
pp. 190 ◽  
Author(s):  
Truong Quoc Phong ◽  
Ngọc Thị Phạm ◽  
Huong Dieu Nguyen ◽  
Anh Thi Ngoc Nguyen
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Aflatoxin B1 ◽  
Bovine Serum ◽  
Test Strip ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Optimal Conditions ◽  
Bicarbonate Buffer ◽  
Ft Ir

Aflatoxins are secondary metabolites mostly produced by Aspergillus flavus and Aspergillus parasiticus and found in agricultural foodstuff such as maize grains, peanuts, animal feeds,... These are toxic and cancerous agents for humans and animals. Among them, aflatoxin B1 is the most consideration due to its highest toxicity and presence in samples. Several methods were developed to detect aflatoxin in food, feed and other foodstuffs such as chromatographic methods (TLC, HPTLC, HPLC), LC-MS/MS, FTIR, RIA, ELISA, SPR, electrochemical, and immunodipstick. Among them only immunodipstick method is compatible for field usage. Therefore, aim of the present study is to determine optimal condition for conjugation of aflatoxin B1 (AFB1) with bovine serum albumin (BSA) to develop a lateral flow immunoassay test strip for detection of aflatoxin B1. Optimal conditions for generating mediate compound of aflatoxin B1 – CMO was investigated: AFB1:CMO ratio of 1:2, reflux temperature of 100oC for 2 hours. Concentration of aflatoxin B1 in reaction was 0.27 µg/µl. Conjugation of AFB1 with CMO was confirmed by thin layer chromatography, HPLC and FT-IR methods. Furthermore, optimal conditions for conjugation of mediate AFB1-CMO derivative with BSA were also investigated. Ratio of AFB1-CMO with BSA was determined as 40:1 and equal to predicted theoretical ratio. Efficient conjugation conditions were 25oC for 2 hours in bicarbonate buffer pH 9.5. Generated conjugate of AFB1-BSA was successfully applied to construct a lateral flow immunoassay test strip for detection of aflatoxin B1

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