scholarly journals Study on Dual Channel Lateral Field Excitation Quartz Crystal Microbalance for Measuring Liquid Electrical Properties

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1253 ◽  
Author(s):  
Jinxing Liang ◽  
Debo Kong ◽  
Chaolin Liu
Keyword(s):  
Mechanical Properties ◽  
Electrical Properties ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Nacl Solution ◽  
Lateral Field ◽  
Practical Applications ◽  
Dual Channel ◽  
Field Excitation ◽  
Organic Solutions

Lateral field excitation quartz crystal microbalance (LFE-QCM) can detect both the electrical properties (conductivity and permittivity) and mechanical properties (viscosity and density) of the liquid. In practical applications for detecting electrical properties, the viscosity and density of the liquid will also change. This research proposed a dual-channel LFE-QCM for reducing the influence of density and viscosity. The sensing layer of one resonant element is almost bare, and the other is covered by a metal film as a reference. Different organic solutions and NaCl solution were used to study the influence of mechanical properties and the temperature on electrical properties. The experimental results demonstrate that the dual-channel LFE-QCM is necessary for properly detecting electrical properties of the liquid.

scholarly journals Quartz Crystal Microbalance with Dissipation Monitoring of Dynamic Viscoelastic Changes of Tobacco BY-2 Cells under Different Osmotic Conditions

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 136
Author(s):  
Zongxing Chen ◽  
Tiean Zhou ◽  
Jiajin Hu ◽  
Haifeng Duan
Keyword(s):  
Mechanical Properties ◽  
Quartz Crystal Microbalance ◽  
Viscoelastic Properties ◽  
Quartz Crystal ◽  
Morphological Changes ◽  
Turgor Pressure ◽  
Plant Cells ◽  
Dynamic Monitoring ◽  
Cellular Structures ◽  
Living Plant

The plant cell mechanics, including turgor pressure and wall mechanical properties, not only determine the growth of plant cells, but also reflect the functional and structural changes of plant cells under biotic and abiotic stresses. However, there are currently no appropriate techniques allowing to monitor the complex mechanical properties of living plant cells non-invasively and continuously. In this work, quartz crystal microbalance with dissipation (QCM-D) monitoring technique with overtones (3–9) was used for the dynamic monitoring of adhesions of living tobacco BY-2 cells onto positively charged N,N-dimethyl-N-propenyl-2-propen-1-aminiumchloride homopolymer (PDADMAC)/SiO2 QCM crystals under different concentrations of mannitol (CM) and the subsequent effects of osmotic stresses. The cell viscoelastic index (CVIn) (CVIn = ΔD⋅n/ΔF) was used to characterize the viscoelastic properties of BY-2 cells under different osmotic conditions. Our results indicated that lower overtones of QCM could detect both the cell wall and cytoskeleton structures allowing the detection of plasmolysis phenomena; whereas higher overtones could only detect the cell wall’s mechanical properties. The QCM results were further discussed with the morphological changes of the BY-2 cells by an optical microscopy. The dynamic changes of cell’s generated forces or cellular structures of plant cells caused by external stimuli (or stresses) can be traced by non-destructive and dynamic monitoring of cells’ viscoelasticity, which provides a new way for the characterization and study of plant cells. QCM-D could map viscoelastic properties of different cellular structures in living cells and could be used as a new tool to test the mechanical properties of plant cells.

scholarly journals Rapid, Simple, and Sensitive Immunoagglutination Assay with SiO2 Particles and Quartz Crystal Microbalance for Quantifying Schistosoma japonicum Antibodies

2006 ◽  
Vol 52 (11) ◽  
pp. 2065-2071 ◽  
Author(s):  
Hua Wang ◽  
Yun Zhang ◽  
Bani Yan ◽  
Li Liu ◽  
Shiping Wang ◽  
...  
Keyword(s):  
Schistosoma Japonicum ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Point Of Care ◽  
Silica Particles ◽  
Rabbit Serum ◽  
Promising Alternative ◽  
Practical Applications ◽  
Sio2 Particles ◽  
Frequency Changes

Abstract Background: The resurgence of the parasitic disease schistosomiasis calls for more efficient diagnostic tests. We developed a rapid, simple, portable, and sensitive immunoagglutination assay that uses SiO2 particles and quartz crystal microbalance (QCM) for quantifying Schistosoma japonicum (Sj) antibodies (SjAb). Methods: We prepared submicrometer-sized silica particles derivatized with Sj antigens as replacements for traditional latex microspheres to specifically agglutinate in the presence of SjAb targets, and we used the QCM monitor to measure the resulting frequency shifts. We optimized the assay medium by adding poly(ethylene glycol) (PEG) as a response accelerator of immunoagglutination. To minimize or eliminate any nonspecific agglutination or adsorption interferences, we conducted appropriate sealing procedures separately for silica particles and the QCM probe. Results: The measured frequency changes were linearly related to the SjAb concentrations in infected rabbit serum. The PEG-assisted immunoagglutination system was quantitatively sensitive to SjAb concentrations ranging from ∼0.70 to 32.31 mg/L, with a detection limit of ∼0.46 mg/L. The obtained linear regression equation was: y = 43.61 x + 80.44 (r = 0.9872). Several serum specimens were evaluated with the developed QCM immunoassay and the results were compared with ELISA, validating the feasibility of practical applications. Conclusions: This novel immunoagglutination-based QCM detection format is rapid, simple to use, and more portable than conventional diagnostic immunoassays, thus offering a promising alternative tool that can be used for point-of-care clinical diagnosis of schistosomiasis, particularly in epidemic situations.

scholarly journals Development of a Flow Injection Based High Frequency Dual Channel Quartz Crystal Microbalance

Sensors ◽  
2017 ◽  
Vol 17 (5) ◽  
pp. 1136 ◽  
Author(s):  
Jinxing Liang ◽  
Jing Zhang ◽  
Wenxiang Zhou ◽  
Toshitsugu Ueda
Keyword(s):  
Quartz Crystal Microbalance ◽  
Flow Injection ◽  
High Frequency ◽  
Quartz Crystal ◽  
Dual Channel
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