scholarly journals Rapid, Simple, and Sensitive Immunoagglutination Assay with SiO2 Particles and Quartz Crystal Microbalance for Quantifying Schistosoma japonicum Antibodies

2006 ◽  
Vol 52 (11) ◽  
pp. 2065-2071 ◽  
Author(s):  
Hua Wang ◽  
Yun Zhang ◽  
Bani Yan ◽  
Li Liu ◽  
Shiping Wang ◽  
...  
Keyword(s):  
Schistosoma Japonicum ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Point Of Care ◽  
Silica Particles ◽  
Rabbit Serum ◽  
Promising Alternative ◽  
Practical Applications ◽  
Sio2 Particles ◽  
Frequency Changes

Abstract Background: The resurgence of the parasitic disease schistosomiasis calls for more efficient diagnostic tests. We developed a rapid, simple, portable, and sensitive immunoagglutination assay that uses SiO2 particles and quartz crystal microbalance (QCM) for quantifying Schistosoma japonicum (Sj) antibodies (SjAb). Methods: We prepared submicrometer-sized silica particles derivatized with Sj antigens as replacements for traditional latex microspheres to specifically agglutinate in the presence of SjAb targets, and we used the QCM monitor to measure the resulting frequency shifts. We optimized the assay medium by adding poly(ethylene glycol) (PEG) as a response accelerator of immunoagglutination. To minimize or eliminate any nonspecific agglutination or adsorption interferences, we conducted appropriate sealing procedures separately for silica particles and the QCM probe. Results: The measured frequency changes were linearly related to the SjAb concentrations in infected rabbit serum. The PEG-assisted immunoagglutination system was quantitatively sensitive to SjAb concentrations ranging from ∼0.70 to 32.31 mg/L, with a detection limit of ∼0.46 mg/L. The obtained linear regression equation was: y = 43.61 x + 80.44 (r = 0.9872). Several serum specimens were evaluated with the developed QCM immunoassay and the results were compared with ELISA, validating the feasibility of practical applications. Conclusions: This novel immunoagglutination-based QCM detection format is rapid, simple to use, and more portable than conventional diagnostic immunoassays, thus offering a promising alternative tool that can be used for point-of-care clinical diagnosis of schistosomiasis, particularly in epidemic situations.

scholarly journals Study on Dual Channel Lateral Field Excitation Quartz Crystal Microbalance for Measuring Liquid Electrical Properties

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1253 ◽  
Author(s):  
Jinxing Liang ◽  
Debo Kong ◽  
Chaolin Liu
Keyword(s):  
Mechanical Properties ◽  
Electrical Properties ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Nacl Solution ◽  
Lateral Field ◽  
Practical Applications ◽  
Dual Channel ◽  
Field Excitation ◽  
Organic Solutions

Lateral field excitation quartz crystal microbalance (LFE-QCM) can detect both the electrical properties (conductivity and permittivity) and mechanical properties (viscosity and density) of the liquid. In practical applications for detecting electrical properties, the viscosity and density of the liquid will also change. This research proposed a dual-channel LFE-QCM for reducing the influence of density and viscosity. The sensing layer of one resonant element is almost bare, and the other is covered by a metal film as a reference. Different organic solutions and NaCl solution were used to study the influence of mechanical properties and the temperature on electrical properties. The experimental results demonstrate that the dual-channel LFE-QCM is necessary for properly detecting electrical properties of the liquid.

scholarly journals Laser Response of a Quartz Crystal Microbalance: Frequency Changes Induced by Light Irradiation in the Air Phase

2009 ◽  
Vol 25 (9) ◽  
pp. 1069-1075 ◽  
Author(s):  
Takayoshi KAWASAKI ◽  
Tetsuhiro MOCHIDA ◽  
Jun-ichi KATADA ◽  
Yoshio OKAHATA
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Light Irradiation ◽  
Frequency Changes

Nano-Scale Tribology Study of Organic Adlayer-Metal Interface Using Quartz Crystal Microbalance Combined With Scanning Tunneling Microscopy

2005 ◽  
Author(s):  
Sang M. Lee ◽  
M. Abdelmaksoud ◽  
J. Krim
Keyword(s):  
Scanning Tunneling Microscopy ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Frictional Heating ◽  
Scanning Tunneling ◽  
Tunneling Microscopy ◽  
Physical Interactions ◽  
Frequency Changes ◽  
Physical Phenomena ◽  
Stm Images

A quartz crystal microbalance combined with scanning tunneling microscopy (STM-QCM) was used to investigate the interactions between organic adlayers (C6H6 and C6H5I) on Cu surfaces and a metallic STM tip. STM images of C6H6 covered Cu surface improved when the QCM was simultaneously oscillated during the imaging. In contrast, STM images of C6H5I covered surfaces became noisy when the sample was oscillated. The two systems moreover exhibited frequency changes of opposite signs in response to STM tip contact, indicative of different physical phenomena at the surface. The dependence of the STM image quality and the frequency shift were interpreted in terms of the adsorbate-substrate chemical and physical interactions, and different levels of frictional heating at the interface.

The Quartz-Crystal-Microbalance Study of Protein Binding on Lipid Monolayers at the Air-Water Interface

MRS Bulletin ◽  
1995 ◽  
Vol 20 (6) ◽  
pp. 52-56 ◽  
Author(s):  
Y. Okahata ◽  
Y. Ebara ◽  
T. Sato
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Time Frame ◽  
Fluorescent Labeling ◽  
Lipid Monolayer ◽  
Lipid Film ◽  
Binding Behavior ◽  
Adsorbed Proteins ◽  
Frequency Changes

Interactions of proteins with cell membranes are of great interest in studies such as molecular recognition at cell surfaces. A monolayer lipid film at the airwater interface is useful in cell-surface modeling. Studies in binding behavior of proteins from a solution with a lipid monolayer have been reported by using various in situ techniques: surface-tension measurements, fluorescent-labeling techniques, radio-labeling techniques, fluorescence reflection methods, surface plasmon resonance, and surface-force measurements. These methods have potential applications in the observation of protein bindings. However, they require large, expensive equipment for in situ measurements and present difficulties for quantitatively obtaining the amount of protein adsorbed and the time frame for both binding and dissociation processes.In this article, we discuss a new, easy in situ technique to detect interactions of adsorbed proteins with a phospholipids monolayer through the use of a Langmuir film balance on which a quartz-crystal microbalance (QCM) is horizontally attached to the lipid monolayer from the air phase (see Figure 1). QCMs are known to be very sensitive mass-measuring devices because their resonance frequency decreases with an increase of a given mass on a QCM at the nanogram level. Adsorption and penetration behavior of proteins can be observed quantitatively from the frequency changes (ΔF) of the QCM on the monolayer and the surface-pressure changes (Δπ) of the monolayer responding to the addition of proteins. The amount of adsorbed proteins (Δm) was also obtained from the frequency changes after lifting and drying the sample in air.Recently, QCMs have become popular tools for detection of bioactive compounds such as odorous and bitter substances, and for measurement of protein adsorption, immuno-assays, DNA hybridization, enzyme reactions, and cell growth.

scholarly journals A Point-of-Care Immunosensor Based on a Quartz Crystal Microbalance with Graphene Biointerface for Antibody Assay

ACS Sensors ◽  
2020 ◽  
Vol 5 (11) ◽  
pp. 3520-3532
Author(s):  
Piramon Hampitak ◽  
Thomas A. Jowitt ◽  
Daniel Melendrez ◽  
Maryline Fresquet ◽  
Patrick Hamilton ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Point Of Care ◽  
Antibody Assay

scholarly journals Blood Coagulation Testing Smartphone Platform Using Quartz Crystal Microbalance Dissipation Method

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 3073 ◽  
Author(s):  
Jia Yao ◽  
Bin Feng ◽  
Zhiqi Zhang ◽  
Chuanyu Li ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Clinical Laboratory ◽  
Point Of Care ◽  
Dissipation Factor ◽  
Parylene C ◽  
Adhesive Surface ◽  
Self Testing ◽  
Coagulation Testing

Blood coagulation function monitoring is important for people who are receiving anticoagulation treatment and a portable device is needed by these patients for blood coagulation self-testing. In this paper, a novel smartphone based blood coagulation test platform was proposed. It was developed based on parylene-C coated quartz crystal microbalance (QCM) dissipation measuring and analysis. The parylene-C coating constructed a robust and adhesive surface for fibrin capturing. The dissipation factor was obtained by measuring the frequency response of the sensor. All measured data were sent to a smartphone via Bluetooth for dissipation calculation and blood coagulation results computation. Two major coagulation indexes, activated partial thromboplastin time (APTT) and prothrombin time (PT) were measured on this platform compared with results by a commercial hemostasis system in a clinical laboratory. The measurement results showed that the adjusted R-square (R2) value for APTT and PT measurements were 0.985 and 0.961 respectively. The QCM dissipation method for blood coagulation measurement was reliable and effective and the platform together with the QCM dissipation method was a promising solution for point of care blood coagulation testing.

A quartz crystal microbalance as a tool for biomolecular interaction studies

RSC Advances ◽  
2015 ◽  
Vol 5 (79) ◽  
pp. 64520-64525 ◽  
Author(s):  
Yuanzi Wu ◽  
Hongwei Ma ◽  
Dayong Gu ◽  
Jian'an He
Keyword(s):  
Ethylene Glycol ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Impedance Analysis ◽  
Biomolecular Interactions ◽  
Poly Ethylene Glycol ◽  
Biomolecular Interaction ◽  
Interaction Studies ◽  
Frequency Changes ◽  
Poly Ethylene

A quartz crystal microbalance was successfully applied to quantitatively analyze biomolecular interactions using a poly(ethylene glycol) matrix and equations for impedance analysis of frequency changes at multiple overtones.

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