scholarly journals Generative Adversarial Networks for Morphological–Temporal Classification of Stem Cell Images

Sensors ◽  
2021 ◽  
Vol 22 (1) ◽  
pp. 206
Author(s):  
Adam Witmer ◽  
Bir Bhanu

Frequently, neural network training involving biological images suffers from a lack of data, resulting in inefficient network learning. This issue stems from limitations in terms of time, resources, and difficulty in cellular experimentation and data collection. For example, when performing experimental analysis, it may be necessary for the researcher to use most of their data for testing, as opposed to model training. Therefore, the goal of this paper is to perform dataset augmentation using generative adversarial networks (GAN) to increase the classification accuracy of deep convolutional neural networks (CNN) trained on induced pluripotent stem cell microscopy images. The main challenges are: 1. modeling complex data using GAN and 2. training neural networks on augmented datasets that contain generated data. To address these challenges, a temporally constrained, hierarchical classification scheme that exploits domain knowledge is employed for model learning. First, image patches of cell colonies from gray-scale microscopy images are generated using GAN, and then these images are added to the real dataset and used to address class imbalances at multiple stages of training. Overall, a 2% increase in both true positive rate and F1-score is observed using this method as compared to a straightforward, imbalanced classification network, with some greater improvements on a classwise basis. This work demonstrates that synergistic model design involving domain knowledge is key for biological image analysis and improves model learning in high-throughput scenarios.

Electronics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 118
Author(s):  
Holly Burrows ◽  
Javad Zarrin ◽  
Lakshmi Babu-Saheer ◽  
Mahdi Maktab-Dar-Oghaz

It is becoming increasingly apparent that a significant amount of the population suffers from mental health problems, such as stress, depression, and anxiety. These issues are a result of a vast range of factors, such as genetic conditions, social circumstances, and lifestyle influences. A key cause, or contributor, for many people is their work; poor mental state can be exacerbated by jobs and a person’s working environment. Additionally, as the information age continues to burgeon, people are increasingly sedentary in their working lives, spending more of their days seated, and less time moving around. It is a well-known fact that a decrease in physical activity is detrimental to mental well-being. Therefore, the need for innovative research and development to combat negativity early is required. Implementing solutions using Artificial Intelligence has great potential in this field of research. This work proposes a solution to this problem domain, utilising two concepts of Artificial Intelligence, namely, Convolutional Neural Networks and Generative Adversarial Networks. A CNN is trained to accurately predict when an individual is experiencing negative emotions, achieving a top accuracy of 80.38% with a loss of 0.42. A GAN is trained to synthesise images from an input domain that can be attributed to evoking position emotions. A Graphical User Interface is created to display the generated media to users in order to boost mood and reduce feelings of stress. The work demonstrates the capability for using Deep Learning to identify stress and negative mood, and the strategies that can be implemented to reduce them.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bastian Rühle ◽  
Julian Frederic Krumrey ◽  
Vasile-Dan Hodoroaba

AbstractWe present a workflow for obtaining fully trained artificial neural networks that can perform automatic particle segmentations of agglomerated, non-spherical nanoparticles from scanning electron microscopy images “from scratch”, without the need for large training data sets of manually annotated images. The whole process only requires about 15 min of hands-on time by a user and can typically be finished within less than 12 h when training on a single graphics card (GPU). After training, SEM image analysis can be carried out by the artificial neural network within seconds. This is achieved by using unsupervised learning for most of the training dataset generation, making heavy use of generative adversarial networks and especially unpaired image-to-image translation via cycle-consistent adversarial networks. We compare the segmentation masks obtained with our suggested workflow qualitatively and quantitatively to state-of-the-art methods using various metrics. Finally, we used the segmentation masks for automatically extracting particle size distributions from the SEM images of TiO2 particles, which were in excellent agreement with particle size distributions obtained manually but could be obtained in a fraction of the time.


Complexity ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-30
Author(s):  
R. Nandhini Abirami ◽  
P. M. Durai Raj Vincent ◽  
Kathiravan Srinivasan ◽  
Usman Tariq ◽  
Chuan-Yu Chang

Computational visual perception, also known as computer vision, is a field of artificial intelligence that enables computers to process digital images and videos in a similar way as biological vision does. It involves methods to be developed to replicate the capabilities of biological vision. The computer vision’s goal is to surpass the capabilities of biological vision in extracting useful information from visual data. The massive data generated today is one of the driving factors for the tremendous growth of computer vision. This survey incorporates an overview of existing applications of deep learning in computational visual perception. The survey explores various deep learning techniques adapted to solve computer vision problems using deep convolutional neural networks and deep generative adversarial networks. The pitfalls of deep learning and their solutions are briefly discussed. The solutions discussed were dropout and augmentation. The results show that there is a significant improvement in the accuracy using dropout and data augmentation. Deep convolutional neural networks’ applications, namely, image classification, localization and detection, document analysis, and speech recognition, are discussed in detail. In-depth analysis of deep generative adversarial network applications, namely, image-to-image translation, image denoising, face aging, and facial attribute editing, is done. The deep generative adversarial network is unsupervised learning, but adding a certain number of labels in practical applications can improve its generating ability. However, it is challenging to acquire many data labels, but a small number of data labels can be acquired. Therefore, combining semisupervised learning and generative adversarial networks is one of the future directions. This article surveys the recent developments in this direction and provides a critical review of the related significant aspects, investigates the current opportunities and future challenges in all the emerging domains, and discusses the current opportunities in many emerging fields such as handwriting recognition, semantic mapping, webcam-based eye trackers, lumen center detection, query-by-string word, intermittently closed and open lakes and lagoons, and landslides.


2022 ◽  
Vol 8 ◽  
Author(s):  
Runnan He ◽  
Shiqi Xu ◽  
Yashu Liu ◽  
Qince Li ◽  
Yang Liu ◽  
...  

Medical imaging provides a powerful tool for medical diagnosis. In the process of computer-aided diagnosis and treatment of liver cancer based on medical imaging, accurate segmentation of liver region from abdominal CT images is an important step. However, due to defects of liver tissue and limitations of CT imaging procession, the gray level of liver region in CT image is heterogeneous, and the boundary between the liver and those of adjacent tissues and organs is blurred, which makes the liver segmentation an extremely difficult task. In this study, aiming at solving the problem of low segmentation accuracy of the original 3D U-Net network, an improved network based on the three-dimensional (3D) U-Net, is proposed. Moreover, in order to solve the problem of insufficient training data caused by the difficulty of acquiring labeled 3D data, an improved 3D U-Net network is embedded into the framework of generative adversarial networks (GAN), which establishes a semi-supervised 3D liver segmentation optimization algorithm. Finally, considering the problem of poor quality of 3D abdominal fake images generated by utilizing random noise as input, deep convolutional neural networks (DCNN) based on feature restoration method is designed to generate more realistic fake images. By testing the proposed algorithm on the LiTS-2017 and KiTS19 dataset, experimental results show that the proposed semi-supervised 3D liver segmentation method can greatly improve the segmentation performance of liver, with a Dice score of 0.9424 outperforming other methods.


2020 ◽  
Author(s):  
Adrian B. Levine ◽  
Jason Peng ◽  
David Farnell ◽  
Mitchell Nursey ◽  
Yiping Wang ◽  
...  

ABSTRACTDeep learning-based computer vision methods have recently made remarkable breakthroughs in the analysis and classification of cancer pathology images. However, there has been relatively little investigation of the utility of deep neural networks to synthesize medical images. In this study, we evaluated the efficacy of generative adversarial networks (GANs) to synthesize high resolution pathology images of ten histological types of cancer, including five cancer types from The Cancer Genome Atlas (TCGA) and the five major histological subtypes of ovarian carcinoma. The quality of these images was assessed using a comprehensive survey of board-certified pathologists (n = 9) and pathology trainees (n = 6). Our results show that the real and synthetic images are classified by histotype with comparable accuracies, and the synthetic images are visually indistinguishable from real images. Furthermore, we trained deep convolutional neural networks (CNNs) to diagnose the different cancer types and determined that the synthetic images perform as well as additional real images when used to supplement a small training set. These findings have important applications in proficiency testing of medical practitioners and quality assurance in clinical laboratories. Furthermore, training of computer-aided diagnostic systems can benefit from synthetic images where labeled datasets are limited (e.g., rare cancers). We have created a publicly available website where clinicians and researchers can attempt questions from the image survey at http://gan.aimlab.ca/.


IEEE Access ◽  
2019 ◽  
Vol 7 ◽  
pp. 111168-111180 ◽  
Author(s):  
Jinrui Wang ◽  
Shunming Li ◽  
Baokun Han ◽  
Zenghui An ◽  
Huaiqian Bao ◽  
...  

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