Characterization of Insulin Mucoadhesive Buccal Films: Spectroscopic Analysis and In Vivo Evaluation

Insulin mucoadhesive buccal films (MBF) are a noninvasive insulin delivery system that offers an advantageous alternative route of administration to subcutaneous injection. One major concern in the formulation of insulin MBF is the preservation of an insulin secondary structure in the presence of the other film components. Buccal films were formulated using chitosan, glycerin, and L-arginine. The MBF-forming solutions (MBF-FS) and the films (MBF) were examined for their chemical and structural stability and for their in vivo activity. Enzyme-Linked Immunosorbent Assay (ELISA) of the insulin-loaded MBF showed that each individualized unit dose was at least loaded with 80% of the insulin theoretical dose. Results of Synchrotron Radiation Circular Dichroism (SRCD) measurements revealed that MBF-FS retained the α-helices and β–sheets conformations of insulin. Fourier transform infrared (FTIR)-microspectroscopy (FTIR-MS) examination of insulin MBF revealed the protective action of L-arginine on insulin structure by interacting with chitosan and minimizing the formation of an unordered structure and β-strand. A blood glucose-lowering effect of insulin MBF was observed in comparison with subcutaneous (S.C) injection using a rat model. As a result; chitosan-based MBFs were formulated and characterized using SRCD and FTIR-MS techniques. Furthermore, the results of in vivo testing suggested the MBFs as a promising delivery system for insulin.

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In-vivo evaluation of cisplatin nanoparticles encompass natural polymer

International Journal of Pharmaceutical Research and Life Sciences ◽

10.26452/ijprls.v6i1.1205 ◽

2020 ◽

Vol 6 (1) ◽

pp. 1-7

Author(s):

Bhavani J ◽

Sunil Kumar Prajapati ◽

Ravichandran S

Keyword(s):

Tumor Volume ◽

Nitrogen Mustard ◽

Common Type ◽

The Body ◽

Nano Particles ◽

Natural Polymer ◽

Drinking Alcohol ◽

In Vivo Evaluation

Cancer is assemblage diseases involving abnormal cell growth amid the potential of spread to other parts of the body due to tobacco use are the cause of about of cancer deaths. Another 10% is due to obesity, poor diet & drinking alcohol. In 2012 about 14.1 million new cases of cancer occurred globally. In females, the most common type is breast cancer. Cisplatin also known as cytophosphane is a nitrogen mustard alkylating agent from the oxazophosphinans groups were used to treat cancers & autoimmune disorders. Based on the above reasons I will fix the aim Preparation characterization of Cisplatin- nano particles & its anticancer activity. Solid tumor volume examination report showed that the assessment of different day indication 15,20,25 & 30th variations of different groups of tumor volumes were decreased CPG Nanoparticles (100 mg/kg)+ DAL(15th day 4.97±0.24↓), (20th day 0.6±0.13↓), (25th day 1.35±0.30↓) & (30th day 1.89±0.13↓).

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Design and In Vivo Characterization of Novel Pulsatile Drug Delivery System of Biguanide Anti-Diabetic

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2020.12.02.0058 ◽

2020 ◽

Vol 12 (02) ◽

Keyword(s):

Drug Delivery ◽

Drug Delivery System ◽

Delivery System ◽

Pulsatile Drug Delivery ◽

In Vivo Characterization

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A Novel Intravaginal Delivery System for Itraconazole: In Vitro and In Vivo Evaluation

Current Drug Delivery ◽

10.2174/156720109787846289 ◽

2009 ◽

Vol 6 (2) ◽

pp. 151-158 ◽

Cited By ~ 2

Author(s):

N. Dobaria ◽

R. Mashru ◽

A. Badhan ◽

A. Thakkar

Keyword(s):

Delivery System ◽

In Vivo Evaluation ◽

Intravaginal Delivery

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Formulation, Characterization and In Vivo Evaluation of Self-Nanoemulsifying Drug Delivery System for Oral Delivery of Valsartan

Current Nanoscience ◽

10.2174/15734137113096660107 ◽

2014 ◽

Vol 10 (2) ◽

pp. 263-270 ◽

Cited By ~ 3

Author(s):

Maulick Chopra ◽

Usha Y. Nayak ◽

Aravind Kumar Gurram ◽

M. Sreenivasa Reddy ◽

K.B. Koteshwara

Keyword(s):

Drug Delivery ◽

Drug Delivery System ◽

Delivery System ◽

Oral Delivery ◽

In Vivo Evaluation

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Design of 99mTc radiolabeled gemcitabine polymeric nanoparticles as drug delivery system and in vivo evaluation

Materials Chemistry and Physics ◽

10.1016/j.matchemphys.2021.124380 ◽

2021 ◽

Vol 263 ◽

pp. 124380

Author(s):

Çiğdem İçhedef ◽

Serap Teksöz ◽

Oğuz Çetin ◽

Burcu Aydın ◽

İbrahim Sarıkavak ◽

...

Keyword(s):

Drug Delivery ◽

Drug Delivery System ◽

Delivery System ◽

Polymeric Nanoparticles ◽

In Vivo Evaluation

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Development of Triamcinolone Acetonide-Loaded Microemulsion as a Prospective Ophthalmic Delivery System for Treatment of Uveitis: In Vitro and In Vivo Evaluation

Pharmaceutics ◽

10.3390/pharmaceutics13040444 ◽

2021 ◽

Vol 13 (4) ◽

pp. 444

Author(s):

Alaa Mahran ◽

Sayed Ismail ◽

Ayat A. Allam

Keyword(s):

Triamcinolone Acetonide ◽

Delivery System ◽

Histopathological Examination ◽

Rabbit Model ◽

Subconjunctival Injection ◽

In Vivo Evaluation ◽

Ternary Phase Diagrams ◽

Ophthalmic Delivery

Treatment of uveitis (i.e., inflammation of the uvea) is challenging due to lack of convenient ophthalmic dosage forms. This work is aimed to determine the efficiency of triamcinolone acetonide (TA)-loaded microemulsion as an ophthalmic delivery system for the treatment of uveitis. Water titration method was used to construct different pseudo-ternary phase diagrams. Twelve microemulsion formulations were prepared using oleic acid, Cremophor EL, and propylene glycol. Among all tested formulations, Formulation F3, composed of oil: surfactant-co-surfactant (1:1): water (15:35:50% w/w, respectively), was found to be stable and showed acceptable pH, viscosity, conductivity, droplet size (211 ± 1.4 nm), and zeta potential (−25 ± 1.7 mV) and almost complete in vitro drug release within 24 h. The in vivo performance of the optimized formulation was evaluated in experimentally uveitis-induced rabbit model and compared with a commercial TA suspension (i.e., Kenacort®-A) either topically or by subconjunctival injection. Ocular inflammation was evaluated by clinical examination, white blood cell count, protein content measurement, and histopathological examination. The developed TA-loaded microemulsion showed superior therapeutic efficiency in the treatment of uveitis with high patient compliance compared to commercial suspension. Hence, it could be considered as a potential ocular treatment option in controlling of uveitis.

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