In-vivo evaluation of cisplatin  nanoparticles encompass natural polymer

Cancer is assemblage diseases involving abnormal cell growth amid the potential of spread to other parts of the body due to tobacco use are the cause of about of cancer deaths. Another 10% is due to obesity, poor diet & drinking alcohol. In 2012 about 14.1 million new cases of cancer occurred globally. In females, the most common type is breast cancer. Cisplatin also known as cytophosphane is a nitrogen mustard alkylating agent from the oxazophosphinans groups were used to treat cancers & autoimmune disorders. Based on the above reasons I will fix the aim Preparation characterization of Cisplatin- nano particles & its anticancer activity. Solid tumor volume examination report showed that the assessment of different day indication 15,20,25 & 30th variations of different groups of tumor volumes were decreased CPG Nanoparticles (100 mg/kg)+ DAL(15th day 4.97±0.24↓), (20th day 0.6±0.13↓), (25th day 1.35±0.30↓) & (30th day 1.89±0.13↓).

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In-vivo evaluation of lipid lowering ability of Chloris paraguaiensis extracts

International Journal of Pharmaceutical Research and Life Sciences ◽

10.26452/ijprls.v7i2.1199 ◽

2020 ◽

Vol 7 (2) ◽

pp. 21-24

Author(s):

Pavani C H

Keyword(s):

Medicinal Plants ◽

Chemical Constituents ◽

The Body ◽

Lipid Lowering ◽

Medical Systems ◽

In Vivo Evaluation ◽

Standard Drug ◽

Anti Oxidant ◽

And Control

Hyperlipidemia is the immediate results of the excessive fat intake in food. This results in the elevated levels of cholesterol and triglycerides in the blood. This leads to heart conditions like CAD, hypertension, congestive heart failure as risk factors which can be lethal. There are many drugs to treat and control the lipids levels in the body. These drugs are either designed to prevent LDL accumulation and VLDL synthesis. Some drugs also lower the elevated levels of saturated lipids in the body. But many drugs are known to cause side effects and adverse effects; therefore, alternatives to the drugs are the subjects for current investigations. Herbs and medicinal plants are used as treatment sources for many years. They have been used in the Indian medical systems like Ayurveda, Siddha etc. As the application of herbs in the treatment is growing, there is an urgent need for the establishment of Pharmacological reasoning and standardization of the activity of the medicinal plants. Chloris paraguaiensis Steud. is Poyaceae member that is called locally as Uppugaddi. Traditionally it is used to treat Rheumatism, Diabetes, fever and diarrhoea. The chemical constituents are known to have anti-oxidant properties and most of the anti-oxidants have anti-hyperlipidemic activity too. Since the plant has abundant flavonoid and phenol content, the current research focusses on the investigation of the anti-hyperlipidemic activity of the plant Chloris extracts. Extracts of Chloris at 200mg/kg showed a comparably similar anti hyperlipidemia activity to that of the standard drug. The extracts showed a dose based increase in the activity at 100 and 200mg/kg body weight.

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Evaluation of New 99mTc(CO)3 + Radiolabeled Glycylglycine In Vivo

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520619666190404154723 ◽

2019 ◽

Vol 19 (11) ◽

pp. 1382-1387

Author(s):

Ahmet M. Şenışık ◽

Çiğdem İçhedef ◽

Ayfer Y. Kılçar ◽

Eser Uçar ◽

Kadir Arı ◽

...

Keyword(s):

High Performance ◽

The Body ◽

Biological Behavior ◽

In Vivo Evaluation ◽

Radiolabeled Peptides ◽

Tumor Bearing ◽

Biodistribution Data ◽

Good Accordance ◽

Rapid Clearance

Background: Peptide-based agents are used in molecular imaging due to their unique properties, such as rapid clearance from the circulation, high affinity and target selectivity. Many of the radiolabeled peptides have been clinically experienced with diagnostic accuracy. The aim of this study was to investigate in vivo biological behavior of [99mTc(CO)3(H2O)3]+ radiolabeled glycylglycine (GlyGly). Methods: Glycylglycine was radiolabeled with a high radiolabeling yield of 94.69±2%, and quality control of the radiolabeling process was performed by thin layer radiochromatography (TLRC) and High-Performance Liquid Radiochromatography (HPLRC). Lipophilicity study for radiolabeled complex (99mTc(CO)3-Gly-Gly) was carried out using solvent extraction. The in vivo evaluation was performed by both biodistribution and SPECT imaging. Results: The high radiolabelling yield of 99mTc(CO)3-GlyGly was obtained and verified by TLRC and HPLRC as well. According to the in vivo results, SPECT images and biodistribution data are in good accordance. The excretion route from the body was both hepatobiliary and renal. Conclusion: This study shows that 99mTc(CO)3-GlyGly has the potential to be used as a peptide-based imaging agent. Further studies, 99mTc(CO)3-GlyGly can be performed on tumor-bearing animals.

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Dual-energy CT for the characterization of urinary calculi: In vitro and in vivo evaluation of a low-dose scanning protocol

European Radiology ◽

10.1007/s00330-009-1300-2 ◽

2009 ◽

Vol 19 (6) ◽

pp. 1553-1559 ◽

Cited By ~ 86

Author(s):

C. Thomas ◽

O. Patschan ◽

D. Ketelsen ◽

I. Tsiflikas ◽

A. Reimann ◽

...

Keyword(s):

Low Dose ◽

Urinary Calculi ◽

Dual Energy ◽

Dual Energy Ct ◽

In Vivo Evaluation ◽

Scanning Protocol

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An Implanted Magnetic Microfluidic Pump for In Vivo Bone Remodeling Applications

Micromachines ◽

10.3390/mi11030300 ◽

2020 ◽

Vol 11 (3) ◽

pp. 300 ◽

Cited By ~ 2

Author(s):

Ziyu Chen ◽

Sunggi Noh ◽

Rhonda D. Prisby ◽

Jeong-Bong Lee

Keyword(s):

Bone Growth ◽

Static Pressure ◽

Dynamic Pressure ◽

The Body ◽

In Vivo Studies ◽

Fischer 344 ◽

Fischer 344 Rat ◽

Pressure Modulation

Modulations of fluid flow inside the bone intramedullary cavity has been found to stimulate bone cellular activities and augment bone growth. However, study on the efficacy of the fluid modulation has been limited to external syringe pumps connected to the bone intramedullary cavity through the skin tubing. We report an implantable magnetic microfluidic pump which is suitable for in vivo studies in rodents. A compact microfluidic pump (22 mm diameter, 5 mm in thickness) with NdFeB magnets was fabricated in polydimethylsiloxane (PDMS) using a set of stainless-steel molds. An external actuator with a larger magnet was used to wirelessly actuate the magnetic microfluidic pump. The characterization of the static pressure of the microfluidic pump as a function of size of magnets was assessed. The dynamic pressure of the pump was also characterized to estimate the output of the pump. The magnetic microfluidic pump was implanted into the back of a Fischer-344 rat and connected to the intramedullary cavity of the femur using a tube. On-demand wireless magnetic operation using an actuator outside of the body was found to induce pressure modulation of up to 38 mmHg inside the femoral intramedullary cavity of the rat.

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Molecular and functional characterization of somatostatin-type signalling in a deuterostome invertebrate

Open Biology ◽

10.1098/rsob.200172 ◽

2020 ◽

Vol 10 (9) ◽

pp. 200172

Author(s):

Ya Zhang ◽

Luis Alfonso Yañez Guerra ◽

Michaela Egertová ◽

Cleidiane G. Zampronio ◽

Alexandra M. Jones ◽

...

Keyword(s):

Functional Characterization ◽

The Body ◽

Pharmacological Characterization ◽

Cardiac Stomach ◽

Physiological Processes ◽

Pharmacological Tests ◽

The Central Nervous System

Somatostatin (SS) and allatostatin-C (ASTC) are structurally and evolutionarily related neuropeptides that act as inhibitory regulators of physiological processes in mammals and insects, respectively. Here, we report the first molecular and functional characterization of SS/ASTC-type signalling in a deuterostome invertebrate—the starfish Asterias rubens (phylum Echinodermata). Two SS/ASTC-type precursors were identified in A. rubens (ArSSP1 and ArSSP2) and the structures of neuropeptides derived from these proteins (ArSS1 and ArSS2) were analysed using mass spectrometry. Pharmacological characterization of three cloned A. rubens SS/ASTC-type receptors (ArSSR1–3) revealed that ArSS2, but not ArSS1, acts as a ligand for all three receptors. Analysis of ArSS2 expression in A. rubens using mRNA in situ hybridization and immunohistochemistry revealed stained cells/fibres in the central nervous system, the digestive system (e.g. cardiac stomach) and the body wall and its appendages (e.g. tube feet). Furthermore, in vitro pharmacological tests revealed that ArSS2 causes dose-dependent relaxation of tube foot and cardiac stomach preparations, while injection of ArSS2 in vivo causes partial eversion of the cardiac stomach. Our findings provide new insights into the molecular evolution of SS/ASTC-type signalling in the animal kingdom and reveal an ancient role of SS-type neuropeptides as inhibitory regulators of muscle contractility.

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Polysialic Acid Modified Liposomes for Improving Pharmacokinetics and Overcoming Accelerated Blood Clearance Phenomenon

Coatings ◽

10.3390/coatings10090834 ◽

2020 ◽

Vol 10 (9) ◽

pp. 834

Author(s):

Xi Han ◽

Ting Zhang ◽

Mengyang Liu ◽

Yanzhi Song ◽

Xinrong Liu ◽

...

Keyword(s):

Blood Circulation ◽

Polysialic Acid ◽

Repeated Administration ◽

The Body ◽

Circulation Time ◽

Blood Clearance ◽

In Vivo Evaluation ◽

Accelerated Blood Clearance ◽

Abc Phenomenon

Poly (ethylene glycol) (PEG) modified nanocarriers are being used widely in the drug delivery system (DDS). However, the “accelerated blood clearance (ABC) phenomenon” was induced upon repeated administration of PEG-modified liposomes, resulting in reduced blood circulation time, and increased accumulation in liver and spleen. To avoid the unexpected phenomenon, polysialic acid (PSA) was selected to modify liposomes. PSA is a natural, highly hydrophilic polysaccharide polymer for which no receptors exists in the body. It is non-immunogenic, biodegradable and endows the conjugated bioactive macromolecule and drugs with increased circulation time in vivo. In the present study, the in vivo evaluation showed that PSA modified liposomes (PSA-Lip) afford extended blood circulation in wistar rats and beagle dogs. Moreover, the ABC phenomenon did not occur and the IgM antibody was not induced after repeated injections of PSA-Lip. These results strongly suggest that PSA modification represents a promising strategy to afford good stealth of the liposomes without evoking the ABC phenomenon.

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Characterization of Albendazole-Randomly Methylated-β-Cyclodextrin Inclusion Complex and In Vivo Evaluation of Its Antihelmitic Activity in a Murine Model of Trichinellosis

PLoS ONE ◽

10.1371/journal.pone.0113296 ◽

2014 ◽

Vol 9 (11) ◽

pp. e113296 ◽

Cited By ~ 12

Author(s):

Agustina García ◽

Darío Leonardi ◽

María D. Vasconi ◽

Lucila I. Hinrichsen ◽

María C. Lamas

Keyword(s):

Inclusion Complex ◽

Murine Model ◽

In Vivo Evaluation ◽

Cyclodextrin Inclusion ◽

Cyclodextrin Inclusion Complex

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Advanced characterizations of nanoparticles for drug delivery: investigating their properties through the techniques used in their evaluations

Nanotechnology Reviews ◽

10.1515/ntrev-2016-0050 ◽

2017 ◽

Vol 6 (4) ◽

pp. 355-372 ◽

Cited By ~ 1

Author(s):

Syed Mahmood ◽

Uttam Kumar Mandal ◽

Bappaditya Chatterjee ◽

Muhammad Taher

Keyword(s):

Drug Delivery ◽

Advanced Characterization ◽

Data Interpretation ◽

Review Article ◽

The Body ◽

Drug Molecule ◽

Drug Molecules ◽

Preparation Techniques

AbstractNanomedicine has achieved a huge success in delivering a wide variety of drug molecules into the target site of the body. In this respect, the characterization of nanoformulation is very important to investigate the drug molecule together with its carrier as a nanoform during formulation, storage, and in vivo transport through the body. This review article summarizes important advanced characterization techniques of nanoformulation with respect to their theories, use of required instrumental parameters, sample preparation techniques, data interpretation, etc., to exploit them for the best possible results. This review article also sheds a glimpse to the shortcomings of these techniques together with further advancements required in future.

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