scholarly journals Epimerization of Deoxynivalenol by the Devosia Strain A6-243 Assisted by Pyrroloquinoline Quinone

Toxins ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 16
Author(s):  
Hui Gao ◽  
Jiafeng Niu ◽  
Hua Yang ◽  
Zhaoxin Lu ◽  
Libang Zhou ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Fusarium Species ◽  
Enzymatic Reaction ◽  
Bacterial Consortium ◽  
Pyrroloquinoline Quinone ◽  
Food Chains ◽  
Pseudomonas Sp ◽  
Biotransformation Process ◽  
Biodegradation Process ◽  
Exogenous Addition

Deoxynivalenol (DON) is a secondary metabolite produced by several Fusarium species that is hazardous to humans and animals after entering food chains. In this study, by adding cofactors, the Devosia strain A6-243 is identified as the DON-transforming bacteria from a bacterial consortium with the ability to biotransform DON of Pseudomonas sp. B6-24 and Devosia strain A6-243, and its effect on the biotransformation process of DON is studied. The Devosia strain A6-243 completely biotransformed 100 μg/mL of DON with the assistance of the exogenous addition of PQQ (pyrroloquinoline quinone) within 48 h and produced non-toxic 3-epi-DON (3-epi-deoxynivalenol), while Pseudomonas sp. B6-24 was not able to biotransform DON, but it had the ability to generate PQQ. Moreover, the Devosia strain A6-243 not only degraded DON, but also exhibited the ability to degrade 3-keto-DON (3-keto-deoxynivalenol) with the same product 3-epi-DON, indicating that DON epimerization by the Devosia strain A6-243 is a two-step enzymatic reaction. The most suitable conditions for the biodegradation process of the Devosia strain A6-243 were a temperature of 16–37 °C and pH 7.0–10, with 15–30 μM PQQ. In addition, the Devosia strain A6-243 was found to completely remove DON (6.7 μg/g) from DON-contaminated wheat. The results presented a reference for screening microorganisms with the ability of biotransform DON and laid a foundation for the development of enzymes for the detoxification of mycotoxins in grain and its products.

scholarly journals Biodegradasi Fosfat pada Limbah Laundry menggunakan Bakteri Consorsium Pelarut Fosfat

2019 ◽  
Vol 18 (1) ◽  
pp. 57
Author(s):  
Zairinayati Rina Zairinayati ◽  
Heri Shatriadi
Keyword(s):  
Standard Deviation ◽  
Statistical Tests ◽  
Bacterial Consortium ◽  
Phosphate Level ◽  
Pseudomonas Sp ◽  
Phosphate Content ◽  
P Values ◽  
Living Things ◽  
Biodegradation Process ◽  
Npk Fertilizer

Latar Belakang: Limbah laundry yang dihasilkan oleh deterjen mengandung bahan aktif yang berbahaya bagi kesehatan mahluk hidup dan lingkungan. Penelitin bertujuan untuk mengurangi kandungan fosfat pada limbah laundry dengan metode biodegradasi dengan menggunakan bakteri yang tergolong dalam consorsium bakteri spesies Bacillu. sp dan Pseudomonas. sp. Sebelum perlakuan bakteri dilakukan equalisasi dengan pupuk NPK dan air gula selama 1 hari setelah itu bakteri siap digunakan sebagai bahan degradasi fosfat dengan 3 perlakuan. Metode penelitian: ini merupakan penelitian eksperimen. Sampel air limbah laundry diambil dari 3 tempat usaha jasa laundry yang berada dikawasan Silaberanti Palembang. Analisis data yang digunakan adalah univariat dengan menyajikan data dalam bentuk tabulasi dan analssis bivariat menggunakan uji Anova untuk melihat perbedaan dari setiap perlakuan.Hasil: menunjukkan  terjadi peningkatan jumlah kadar fosfat sebelum dilakukan proses biodegradasi yakni 9,8 m/L sebelum dilakukan penambahan baketeri menjadi Rata-rata kadar fosfat pada penambahan bakteri 50 ml adalah 27,13 dengan standar deviasi 2,73. Pada dosis penambahan bakteri 100 ml rata-rata kadar fosfat adalah 23,87 dengan standar deviasi 2,08 dan pada dosis penambahan bakteri 150 ml rata-rata kadar fosfat 22,62 dengan standar deviasi 4,41, dan hasil uji statistic didapat nilai p = 0,306 berarti pada alpha 5%.Simpulan: bahwa tidak ada perbedaan penambahan bakteri dengan berbagai dosis dengan kadar fosfat pada air limbah laundry. ABSTRACT Title: Phosphate Biodegradation in Laundry Waste Using Consorsium Bacteria PhosphateBackground: Laundry waste produced by detergent contains active ingredients that are harmful to the health of living things and environment. Research aims to reduce phosphate content in laundry waste by biodegradation method by using bacteria belonging to the bacterial consortium of Bacillu species. sp and Pseudomonas. sp. Before the treatment of the bacteria was equalized by adding NPK fertilizer and sugar liquid for 1 day and the bacteria were ready to be used as phosphate degradation material with 3 treatments.Methods: of this research is experimental research. Laundry waste water samples were taken from 3 laundry service located in the Silaberanti area of Palembang. Data analysis used univariate by presenting data in the form of tabulations and bivariate analssis using the ANOVA test to see differences in each treatment.Results: showed an increase in number of phosphate levels before the biodegradation process of 9.8 m / L before adding the bakeries to the average phosphate level in the addition of 50 ml bacteria was 27.13 with a standard deviation of 2.73. At the dose of adding 100 ml of bacteria the average phosphate level was 23.87 with a standard deviation of 2.08 and at the dose of addition of 150 ml bacteria the average phosphate level was 22.62 with a standard deviation of 4.41, and the results of statistical tests obtained p values = 0.306 means at alpha 5%Conclusion: : is that there is no difference in the addition of bacteria with various doses with phosphate levels in laundry wastewater. 

scholarly journals Possibility of Using Bacteria-Destructors for Cypermetrin Degradation

2021 ◽  
pp. 556-563
Author(s):  
Diyorbek Kosimov ◽  
Lyudmila Zaynitdinova ◽  
Roxila Jo’rayeva ◽  
Svetlana Kukanova ◽  
Rustam Ergashev ◽  
...  
Keyword(s):  
Laboratory Experiments ◽  
Bacterial Consortium ◽  
Biochemical Properties ◽  
Pseudomonas Sp

From soils artificially contaminated with cypermethrin, 3 isolates were obtained that were resistant to cypermethrin concentrations of 40 mg/kg soil, and their morphological, cultural and biochemical properties were studied. On the basis of the obtained strains, a bacterial consortium was developed, which consists of the cultures of Pseudomonas sp., Bacilus sp. and Ochrobactrum sp. Laboratory experiments on the decomposition of cypermethrin showed the effectiveness of this consortium, within 28 days the decomposition of cypermethrin (40 mg/kg) was 100%.

Nitrate removal efficiency of bacterial consortium (Pseudomonas sp. KW1 and Bacillus sp. YW4) in synthetic nitrate-rich water

2008 ◽  
Vol 157 (2-3) ◽  
pp. 553-563 ◽  
Author(s):  
Sundaram Rajakumar ◽  
Pudukadu Munusamy Ayyasamy ◽  
Kuppusamy Shanthi ◽  
Palanisami Thavamani ◽  
Palanivel Velmurugan ◽  
...  
Keyword(s):  
Removal Efficiency ◽  
Nitrate Removal ◽  
Bacterial Consortium ◽  
Pseudomonas Sp ◽  
Bacillus Sp

OBTAINING A LIPOPHILIC BACTERIAL CONSORTIUM FOR FAT DEPOSITS UTILIZATION

2020 ◽  
Author(s):  
A.L. Gerasimchuk ◽  
◽  
D.V. Antsiferov ◽  
P.A. Bukhtiyarova ◽  
D.A. Ivasenko ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Industrial Wastewater ◽  
Food Industry ◽  
Bacterial Consortium ◽  
Pseudomonas Sp ◽  
Bacterial Strains ◽  
Fat Deposits ◽  
Pseudomonas Nitroreducens ◽  
Pseudomonas Extremaustralis

Bacterial strains with lipolytic activities Pseudomonas citronellolis A13, Pseudomonas extremaustralis Б11, Pseudomonas nitroreducens Д14, Pseudomonas sp. B21, Microvirgula curvata B37, Brevibacillus sp. 28Д, Bacillus subtilis spp. B10, B34 were isolated from fat-containing industrial wastewater of food industry and were used for obtaining a lipophilic bacterial consortium that perspective for fat deposits utilization.

Optimisation of Key Abiotic Factors of PAH (Naphthalene, Phenanthrene and Anthracene) Biodegradation Process by a Bacterial Consortium

2010 ◽  
Vol 217 (1-4) ◽  
pp. 365-374 ◽  
Author(s):  
Raquel Simarro ◽  
Natalia González ◽  
Luis Fernando Bautista ◽  
Raquel Sanz ◽  
M. Carmen Molina
Keyword(s):  
Abiotic Factors ◽  
Bacterial Consortium ◽  
Biodegradation Process
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