On the Suitability of a Bacterial Consortium To Implement a Continuous PAHs Biodegradation Process in a Stirred Tank Bioreactor

2012 ◽  
Vol 51 (49) ◽  
pp. 15895-15900 ◽  
Author(s):  
F. Moscoso ◽  
I. Teijiz ◽  
M. A. Sanromán ◽  
F. J. Deive
2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Roselei Claudete Fontana ◽  
Simone Mendonça ◽  
Félix Gonçalves de Siqueira ◽  
Aldo José Pinheiro Dillon ◽  
...  

2019 ◽  
Vol 117 ◽  
pp. 113-125 ◽  
Author(s):  
Zorana Rončević ◽  
Jovana Grahovac ◽  
Siniša Dodić ◽  
Damjan Vučurović ◽  
Jelena Dodić

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Mohd Azmir Arifin ◽  
Maizirwan Mel ◽  
Mohamed Ismail Abdul Karim ◽  
Aini Ideris

The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell number was yielded by culture of Vero cells in Dulbecco's Modified Eagle Medium (DMEM) which was1.93×106 cells/ml. Secondly Vero cells were grown in two-litre stirred tank bioreactor by using several commercial microcarriers. We achieved the maximum cell concentration about7.95×105 cells/ml when using Cytodex 1. Later we produced Newcastle Disease virus in stirred tank bioreactor based on the design developed using Taguchi L4 method. Results reveal that higher multiplicity of infection (MOI) and size of cell inoculums can yield higher virus titer. Finally, virus samples were purified using high-speed centrifugation based on3∗∗(3-1) Fractional Factorial Design. Statistical analysis showed that the maximum virus titer can be achieved at virus sample concentration of 58.45% (v/v), centrifugation speed of 13729 rpm, and centrifugation time of 4 hours. As a conclusion, high yield of virus titer could be achieved through optimization of cell culture in bioreactor and separation by high-speed centrifugation.


2008 ◽  
Vol 21 (3) ◽  
pp. 786-792 ◽  
Author(s):  
Gabriela Trejo-Tapia ◽  
Carlos M. Cerda-García-Rojas ◽  
Mario Rodríguez-Monroy ◽  
Ana C. Ramos-Valdivia

2017 ◽  
Vol 243 ◽  
pp. 264-272 ◽  
Author(s):  
Larissa Pereira Brumano ◽  
Felipe Antonio Fernandes Antunes ◽  
Sara Galeno Souto ◽  
Júlio Cesar dos Santos ◽  
Joachim Venus ◽  
...  

2018 ◽  
Vol 26 ◽  
pp. 80-87 ◽  
Author(s):  
Dão P. de Carvalho Neto ◽  
Gilberto V. de Melo Pereira ◽  
Ana M.O. Finco ◽  
Luiz A.J. Letti ◽  
Bruno J.G. da Silva ◽  
...  

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