scholarly journals Detection of Pathogenic Leptospires in Water and Soil in Areas Endemic to Leptospirosis in Nicaragua

2020 ◽  
Vol 5 (3) ◽  
pp. 149 ◽  
Author(s):  
Byron Flores ◽  
Karla Escobar ◽  
José Luis Muzquiz ◽  
Jessica Sheleby-Elías ◽  
Brenda Mora ◽  
...  

In Nicaragua, there are ideal environmental conditions for leptospirosis. The objective of this investigation was to detect pathogenic and saprophytic leptospires in water and soil samples from leptospirosis-endemic areas in Nicaragua. Seventy-eight water and 42 soil samples were collected from houses and rivers close to confirmed human cases. Leptospira spp was isolated in Ellinghausen–McCullough–Johnson–Harris (EMJH) culture medium with 5-fluororacil and positive samples were analyzed through PCR for the LipL32 gene, specific for pathogenic leptospires (P1 clade). There were 73 positive cultures from 120 samples, however only six of these (5% of all collected samples) were confirmed to be pathogenic, based on the presence of the LipL32 gene (P1 clade). Of these six pathogenic isolates, four were from Leon and two from Chinandega. Four pathogenic isolates were obtained from water and two from soil. This study proved the contamination of water and soil with pathogenic leptospires, which represents a potential risk for public health.

2007 ◽  
Vol 146 (1-2) ◽  
pp. 137-141 ◽  
Author(s):  
C. Sivani ◽  
G. Ramakrishna Naidu ◽  
J. Narasimhulu ◽  
D. Rekha ◽  
J. Dilip Kumar ◽  
...  

2021 ◽  
Vol 49 (1) ◽  
Author(s):  
Abdullahi Tunde Aborode ◽  
Ana Carla dos Santos Costa ◽  
Anmol Mohan ◽  
Samarth Goyal ◽  
Aishat Temitope Rabiu ◽  
...  

AbstractThe plague has been wreaking havoc on people in Madagascar with the COVID-19 pandemic. Madagascar’s healthcare sector is striving to respond to COVID-19 in the face of a plague outbreak that has created a new strain on the country’s public health system. The goal and activities of the gradual epidemic of plague in Madagascar during COVID-19 are described in this research. In order to contain the plague and the COVID-19 pandemic in this country, we have suggested long-term recommendations that can help to contain the outbreak so that it may spread to non-endemic areas.


Heliyon ◽  
2021 ◽  
pp. e07715
Author(s):  
Punam Thapa ◽  
Alina Thapa ◽  
Sujan Khadka ◽  
Sanjeep Sapkota ◽  
Om Prakash Panta ◽  
...  

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