Selection of Potential Therapeutic Bacteriophages that Lyse a CTX-M-15 Extended Spectrum β-Lactamase Producing Salmonella enterica Serovar Typhi Strain from the Democratic Republic of the Congo

ABSTRACT A chloramphenicol-resistant strain of Salmonella enterica serovar Typhi was first noted in Korea in 1992, when a resistant isolate was detected in a returned traveler. Continued isolation of multidrug-resistant (MDR) strains thereafter in other settings prompted a retrospective analysis of laboratory records and phenotypic and genotypic analyses of 12 chloramphenicol-resistant isolates. Among these, one isolate was resistant only to chloramphenicol, and the other isolates were also resistant to ampicillin and co-trimoxazole. MDR was transferred by conjugation from 9 of the 11 isolates. PCR showed that all isolates had an incompatible group HI1 plasmid, and oriT was detected in 10 isolates, which included strains with an unsuccessful transfer of resistance. All of the ampicillin-resistant isolates had a β-lactamase band of pI 5.4 and bla TEM alleles. A PCR amplicon from an isolate showed that the sequences were identical to those of bla TEM-1, suggesting that all isolates had a TEM-1 β-lactamase. All isolates had class 1 integrons: 10 isolates had integrons of ca. 1.2 kb with dhfr7 gene cassettes, and 1 isolate had an integron of ca. 2.3 kb with aacA4 and bla OXA-1-like gene cassettes. The pulsed-field gel electrophoresis patterns of 7 of 11 MDR isolates were identical and indistinguishable from those reported for isolates in India and Indonesia. In conclusion, some of the MDR strains in Korea are related to those in other Asian countries. Susceptibility testing became necessary for selection of antimicrobial agents for the optimal treatment of patients with the emergence of MDR Salmonella serovar Typhi in Korea.

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Typhoid Fever due to Extended Spectrum β-Lactamase-Producing Salmonella enterica Serovar Typhi: A Case Report and Literature Review

Case Reports in Infectious Diseases ◽

10.1155/2018/4610246 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5 ◽

Cited By ~ 4

Author(s):

Abdul Azeez Ahamed Riyaaz ◽

Vindya Perera ◽

Sabaratnam Sivakumaran ◽

Nelun de Silva

Keyword(s):

Case Report ◽

Sri Lanka ◽

Typhoid Fever ◽

Salmonella Enterica ◽

Salmonella Enterica Serovar Typhi ◽

Extended Spectrum ◽

Cephalosporin Resistance ◽

Resistant Strains ◽

Derivatives Of

Emergence of cephalosporin-resistant strains of Salmonella enterica serovar Typhi is a cause of concern in the management of enteric fever. Cephalosporin resistance in Salmonella species is mainly due to the production of extended-spectrum β-lactamases (ESBLs). The majority of ESBLs in Salmonella enterica serovar Typhi are derivatives of the TEM, SHV, and CTX-M β-lactamase families. Of these, CTX-M appears to be predominant. This paper discusses the detection and molecular characterization of an ESBL-producing Salmonella enterica serovar Typhi strain isolated from a patient who was admitted to a private hospital in Sri Lanka. The three main types of β-lactamases such as TEM, SHV, and CTX-M were identified in this isolate. This case report from Sri Lanka contributes to the knowledge of the increasingly reported cases of typhoid fever due to Salmonella enterica serovar Typhi resistant to β-lactamase by ESBL production.

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Salmonella enterica serovar Typhi strain producing extended-spectrum β-lactamases in Dhaka, Bangladesh

Journal of Medical Microbiology ◽

10.1099/jmm.0.044065-0 ◽

2012 ◽

Vol 61 (7) ◽

pp. 1032-1033 ◽

Cited By ~ 18

Author(s):

Dilruba Ahmed ◽

Ashfaqul Hoque ◽

Razib Mazumder ◽

Kamrun Nahar ◽

Nazrul Islam ◽

...

Keyword(s):

Salmonella Enterica ◽

Salmonella Enterica Serovar Typhi ◽

Extended Spectrum

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Plasmid-Mediated Resistance to Cephalosporins in Salmonella enterica Serovar Typhi

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00225-10 ◽

2010 ◽

Vol 54 (9) ◽

pp. 3991-3992 ◽

Cited By ~ 9

Author(s):

Masatomo Morita ◽

Nobuko Takai ◽

Jun Terajima ◽

Haruo Watanabe ◽

Manabu Kurokawa ◽

...

Keyword(s):

Salmonella Enterica ◽

Salmonella Enterica Serovar Typhi ◽

Extended Spectrum ◽

Cephalosporin Resistance

ABSTRACT This study characterized a cephalosporin-resistant Salmonella enterica serovar Typhi isolate. The organism possessed a plasmid encoding the CTX-M-15 extended-spectrum-β-lactamase. This plasmid is the determinant for the phenotype of cephalosporin resistance and is transferrable among Enterobacteriaceae.

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Selection of Salmonella enterica Serovar Typhi Genes Involved during Interaction with Human Macrophages by Screening of a Transposon Mutant Library

PLoS ONE ◽

10.1371/journal.pone.0036643 ◽

2012 ◽

Vol 7 (5) ◽

pp. e36643 ◽

Cited By ~ 24

Author(s):

Sébastien C. Sabbagh ◽

Christine Lepage ◽

Michael McClelland ◽

France Daigle

Keyword(s):

Salmonella Enterica ◽

Mutant Library ◽

Human Macrophages ◽

Salmonella Enterica Serovar Typhi ◽

Selection Of

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Plasmid Carriage and ESBL Production among Salmonella Enterica Serovar Typhi from Some Parts of Adamawa State, Nigeria

UMYU Journal of Microbiology Research (UJMR) ◽

10.47430/ujmr.2161.006 ◽

2021 ◽

Vol 6 (1) ◽

pp. 47-54

Author(s):

M. Sale ◽

◽

M. I. Ja'afaru ◽

S. M. Pukuma

Keyword(s):

Salmonella Enterica ◽

Stool Culture ◽

Pcr Analysis ◽

Warning Sign ◽

Beta Lactamase ◽

Esbl Production ◽

Extended Spectrum Beta Lactamase ◽

Salmonella Enterica Serovar Typhi ◽

Extended Spectrum

Effective treatment of typhoid fever caused by Salmonella enterica serovar Typhi has been hampered by the emergence of multidrug resistant and extended spectrum beta lactamase producing strains thus making the organism an important public health pathogen especially in developing countries. This study was aimed at screening Salmonella ser. Typhi isolates from suspected enteric fever patients for the presence of plasmids as well as ESBL production. Eightyfour (84) Salmonella ser. Typhi isolates were obtained from blood and stool culture giving a prevalence of 17.5%. Results of susceptibility screening revealed that 37.5% of the isolates demonstrated ability to produce extended spectrum beta lactamase in vitro out which 37.5% were isolates from Yola while 40% were isolates recovered from Gombi. Furthermore, 41(48.8 %) of the isolates from this study bore plasmids out of which 25(47.1 %) were from Yola metropolis while 13 (41.9 %) were from Gombi Local Government Area. The most common antibiotic resistant marker borne on the plasmids carried by S. ser. Typhi in the study area was resistance to AmpC Co (20) followed by resistance gene for ceftriaxone 10. Screening of the isolates for extended spectrum beta lactamase activity using the double disk synergy test revealed that 9 isolates namely; Salmonella ser. Typhi isolates GMB1, 6, 13, 26 and Salmonella ser. Typhi YLA 4, 11, 22, 31 and 42 showed positive ESBL activity. The PCR analysis revealed the presence of Blactx gene cluster in 4/25 (16%) of S. Typhi isolates. The detection of some strains with resistance to ceftriaxone as well as the ability to produce the ESBL is an early warning sign indicating the need for more controlled use of this drug in the country, especially in the study area. Keywords: Plasmid, Resistance, ESBL

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Prevalence and gene sequencing of extended spectrum -lactamases producing Salmonella enterica serovar. Typhi from South-East Nigeria

African Journal of Pharmacy and Pharmacology ◽

10.5897/ajpp2020.5115 ◽

2020 ◽

Vol 14 (7) ◽

pp. 192-202

Author(s):

Ejikeme Onyenwe Nathaniel ◽

Didacus Nnamani Nnabuike ◽

Chidi Okoro Jude ◽

Nnenna Nwofor Chioma ◽

Jesumirhewe Christiana

Keyword(s):

Salmonella Enterica ◽

Gene Sequencing ◽

Salmonella Enterica Serovar Typhi ◽

Extended Spectrum

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Genomic Dissection of Travel-Associated Extended-Spectrum-Beta-Lactamase-Producing Salmonella enterica Serovar Typhi Isolates Originating from the Philippines: a One-Off Occurrence or a Threat to Effective Treatment of Typhoid Fever?

Journal of Clinical Microbiology ◽

10.1128/jcm.03104-14 ◽

2014 ◽

Vol 53 (2) ◽

pp. 677-680 ◽

Cited By ~ 9

Author(s):

Rene S. Hendriksen ◽

Pimlapas Leekitcharoenphon ◽

Matthew Mikoleit ◽

Jacob Dyring Jensen ◽

Rolf Sommer Kaas ◽

...

Keyword(s):

Typhoid Fever ◽

Salmonella Enterica ◽

The Philippines ◽

Whole Genome Sequence ◽

Whole Genome ◽

Beta Lactamase ◽

Content Type ◽

Extended Spectrum Beta Lactamase ◽

Salmonella Enterica Serovar Typhi ◽

Extended Spectrum

One unreported case of extended-spectrum-beta-lactamase (ESBL)-producingSalmonella entericaserovar Typhi was identified, whole-genome sequence typed, among other analyses, and compared to other available genomes ofS. Typhi. The reported strain was similar to a previously published strain harboringblaSHV-12from the Philippines and likely part of an undetected outbreak, the first of ESBL-producingS. Typhi.

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