scholarly journals Unexpected Genetic Diversity of Two Novel Swine MRVs in Italy

Viruses ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 574 ◽  
Author(s):  
Lara Cavicchio ◽  
Luca Tassoni ◽  
Gianpiero Zamperin ◽  
Mery Campalto ◽  
Marilena Carrino ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Amino Acid ◽  
Molecular Characterization ◽  
First Report ◽  
Genetic Relation ◽  
Genetic Groups ◽  
The Family ◽  
Dsrna Viruses ◽  
Unique Amino Acid

Mammalian Orthoreoviruses (MRV) are segmented dsRNA viruses in the family Reoviridae. MRVs infect mammals and cause asymptomatic respiratory, gastro-enteric and, rarely, encephalic infections. MRVs are divided into at least three serotypes: MRV1, MRV2 and MRV3. In Europe, swine MRV (swMRV) was first isolated in Austria in 1998 and subsequently reported more than fifteen years later in Italy. In the present study, we characterized two novel reassortant swMRVs identified in one same Italian farm over two years. The two viruses shared the same genetic backbone but showed evidence of reassortment in the S1, S4, M2 segments and were therefore classified into two serotypes: MRV3 in 2016 and MRV2 in 2018. A genetic relation to pig, bat and human MRVs and other unknown sources was identified. A considerable genetic diversity was observed in the Italian MRV3 and MRV2 compared to other available swMRVs. The S1 protein presented unique amino acid signatures in both swMRVs, with unexpected frequencies for MRV2. The remaining genes formed distinct and novel genetic groups that revealed a geographically related evolution of swMRVs in Italy. This is the first report of the complete molecular characterization of novel reassortant swMRVs in Italy and Europe, which suggests a greater genetic diversity of swMRVs never identified before.

scholarly journals Prevalence and Genetic Diversity of Aichi Viruses in Wastewater and River Water in Japan

2011 ◽  
Vol 77 (6) ◽  
pp. 2184-2187 ◽  
Author(s):  
Masaaki Kitajima ◽  
Eiji Haramoto ◽  
Chanetta Phanuwan ◽  
Hiroyuki Katayama
Keyword(s):  
Genetic Diversity ◽  
River Water ◽  
Molecular Characterization ◽  
Water Samples ◽  
Aichi Virus ◽  
Aquatic Environments ◽  
First Report ◽  
River Water Samples ◽  
Genotype A

ABSTRACTAichi virus (AiV) genomes were detected in 12 (100%) influent and 11 (92%) effluent wastewater and 36 (60%) river water samples. Among 260 strains identified, 255 were genotype A and 5 were genotype B. This is the first report describing the molecular characterization of AiVs in aquatic environments in Japan.

scholarly journals Molecular detection and phylogenetic analysis of pigeon circovirus from racing pigeons in China during 2016-2019

2021 ◽  
Author(s):  
Haoran Wang ◽  
Hui Gao ◽  
Zhiwen Jiang ◽  
Leibo Shi ◽  
Pengwei Zhao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Amino Acid ◽  
Protein A ◽  
Columba Livia ◽  
High Genetic Diversity ◽  
Rep Proteins ◽  
The Family ◽  
Positive Rate ◽  
Unique Amino Acid

The Pigeon circovirus (PiCV) containing a circular single stranded DNA (approximately 2 kb) genome belongs to the genus Circovirus of the family Circoviridae. PiCV infections in pigeons ( Columba livia) have been reported worldwide. Currently, pigeon racing is becoming increasingly popular and considered to be a national sport in China, and even, the greatest competitions of racing pigeons are taking place in China. However, there are still no epidemiologic data regarding PiCV infections among racing pigeons in China. To trace the prevalence, genetic variation and evolution of PiCV in sick and healthy racing pigeons, 622 samples were collected from 11 provinces or municipalities in China from 2016 to 2019. Samples were tested by polymerase chain reaction. The results showed that the positive rate of PiCV was 19.3% (120/622) at the sample level and 59.0% (23/39) at the club level, thus suggesting that the virus was prevalent in Chinese racing pigeons. A sequence analysis revealed that the cap genes of the PiCV strains identified in our study displayed a high genetic diversity and shared nucleotide homologies of 71.9%–100% and amino acid homologies of 71.7%–100%. 28 and 36 unique amino acid substitutions were observed in the Cap and Rep proteins derived from our PiCV strains, respectively. Furthermore, The ATT and GTG were highly suspected to be the start codons of the Cap protein. A cap-gene-based phylogenetic analysis showed that the strains in this study could be further divided into seven clades (A, B, C, E, G, H, and I) and some of them were closely related to worldwide strains from different types of pigeons. A large number of recombination events (31 events) were also detected in the PiCV genomes from Chinese racing pigeons. These findings indicate that PiCV strains circulating in China exhibit a high genetic diversity.

Molecular characterization of echovirus 13 uncovering high genetic diversity and identification of new genotypes in Pakistan

2017 ◽  
Vol 48 ◽  
pp. 102-108
Author(s):  
Shahzad Shaukat ◽  
Mehar Angez ◽  
Tariq Mahmood ◽  
Muhammad Masroor Alam ◽  
Salmaan Sharif ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
High Genetic Diversity

scholarly journals Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

2015 ◽  
Vol 45 (12) ◽  
pp. 2197-2200 ◽  
Author(s):  
Thor Vinícius Martins Fajardo ◽  
Monique Bezerra Nascimento ◽  
Marcelo Eiras ◽  
Osmar Nickel ◽  
Gilvan Pio-Ribeiro
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Molecular Analysis ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Causal Agent ◽  
Ringspot Virus ◽  
Prunus Necrotic Ringspot Virus ◽  
First Time

ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

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