scholarly journals Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)

Viruses ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1713
Author(s):  
Alain Moïse Onihary ◽  
Iony Manitra Razanajatovo ◽  
Lydia Rabetafika ◽  
Alexandra Bastaraud ◽  
Jean-Michel Heraud ◽  
...  
Keyword(s):  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
Macrobrachium Rosenbergii ◽  
Culture Industry ◽  
White Spot ◽  
Type I ◽  
Shrimp Culture ◽  
White Spot Disease ◽  
North West ◽  
White Spot Syndrome

White Spot Disease (WSD) caused by the White Spot Syndrome Virus (WSSV) is the most devastating viral disease threatening the shrimp culture industry worldwide, including Madagascar. WDS was first reported on the island in 2012; however, little is known about the circulation of the virus and its genetic diversity. Our study aimed at describing the molecular diversity and the spread of WSSV in the populations of Madagascan crustaceans. Farmed and wild shrimps were collected from various locations in Madagascar from 2012 to 2016 and were tested for WSSV. Amplicons from positive specimens targeting five molecular markers (ORF75, ORF94, ORF125, VR14/15 and VR23/24) were sequenced for genotyping characterizations. Four genotypes were found in Madagascar. The type-I genotype was observed in the south-west of Madagascar in April 2012, causing a disastrous epidemic, then spread to the North-West coast. Type-II strains were detected in October 2012 causing an outbreak in another Penaeus monodon farm. In 2014 and 2015, types II and III were observed in shrimp farms. Finally, in 2016, types II and IV were found in wild species including Fenneropenaeus indicus, Metapenaeus monoceros, Marsupenaeus japonicus and Macrobrachium rosenbergii. Considering the economic importance of the shrimp industry for Madagascar, our study highlights the need to maintain WSSV surveillance to quickly take appropriate countermeasures in case of outbreak and to sustain this industry.

scholarly journals Occurrence of white spot syndrome virus and Vibrio parahaemolyticus in brackishwater shrimp culture systems of Sundarban, West Bengal, India

2017 ◽  
Vol 64 (4) ◽  
Author(s):  
Sanjoy Das ◽  
Tapas Kumar Ghoshal ◽  
Gouranga Biswas
Keyword(s):  
Vibrio Parahaemolyticus ◽  
White Spot Syndrome Virus ◽  
West Bengal ◽  
White Spot ◽  
Human Beings ◽  
Crop Failure ◽  
Shrimp Culture ◽  
White Spot Disease ◽  
Culture Systems ◽  
White Spot Syndrome

White spot disease (WSD) and acute hepatopancreatic necrosis disease (AHPND) are two important and highly fatal diseases of cultured shrimps. The occurrence of white spot syndrome virus (WSSV) was found to be very high in brackishwateraquaculture systems of Sundarban area in West Bengal, with 40.6% of the samples being positive of which 18.75% being positive in the first step PCR. The prevalence of WSSV in traditional and semi-intensive culture systems was foundas 59.3 and 27%, respectively. On the other hand, presence of Vibrio parahaemolyticus was detected in 53.1, 55.5 and 75.9% of the shrimp, water and soil samples, respectively. All the isolates of V. parahaemolyticus contained toxR gene as detected by PCR. However, none of the isolates was realised as AHPND causing isolate by PCR. Moreover, tdh gene, which causes gastroenteritis in human beings, was also not detected in any of the isolates. The present study indicated that WSSV is the major cause of crop failure in the brackishwater shrimp culture systems of Sundarban, India. Although,V. parahaemolyticus is highly prevalent in brackishwater shrimp culture systems of Sundarban, strains causing gastroenteritis or AHPND were not detected during the present study.

White spot syndrome virus (WSSV) and prevalence of disease resistance in a commercially cultured population of Penaeus monodon Fabricius, 1798 (Decapoda, Dendrobranchiata)

Crustaceana ◽  
2014 ◽  
Vol 87 (14) ◽  
pp. 1593-1605 ◽  
Author(s):  
U. Chakrabarty ◽  
S. Dutta ◽  
A. Mallik ◽  
N. Mandal
Keyword(s):  
Disease Resistance ◽  
White Spot Syndrome Virus ◽  
Health Management ◽  
Penaeus Monodon ◽  
White Spot ◽  
White Spot Disease ◽  
Disease Resistant ◽  
Aquaculture Industry ◽  
White Spot Syndrome ◽  
Shrimp Health

White spot disease (WSD) caused by the white spot syndrome virus (WSSV) is the leading cause for huge economic destruction in the shrimp aquaculture industry. The present study investigates the prevalence of WSSV and disease-resistant shrimps among a commercially cultured population of Penaeus monodon Fabricius, 1798. Results suggest that WSSV prevalence among 32 culture ponds affected by various diseases was 58.2% cumulatively in 2009 and 2010. Real-time PCR yielded 1.46 × 108, 1.94 × 106 and 3.6 × 104 copies of WSSV per μg of genomic DNA of shrimp in severe, moderate and low infected shrimps, successively. Among the shrimps collected, the prevalence of disease resistance was 31.4% and 37.1% consecutively in 2009 and 2010. It was found that 34% of the shrimps were disease resistant while considering both years. This study shows the importance of using disease-resistant seeds for better shrimp health management.

scholarly journals Co-Infection of Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) and White Spot Syndrome Virus (WSSV) in the Wild Crustaceans of Andaman and Nicobar Archipelago, India

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1378
Author(s):  
Kandasamy Saravanan ◽  
Jayasimhan Praveenraj ◽  
Rajendran Kiruba-Sankar ◽  
Varsha Devi ◽  
Utpal Biswas ◽  
...  
Keyword(s):  
Inclusion Bodies ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Scylla Serrata ◽  
Histopathological Analysis ◽  
Necrosis Virus ◽  
High Sequence Identity ◽  
In The Wild ◽  
White Spot Syndrome

The present study was intended to screen the wild crustaceans for co-infection with Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) and White Spot Syndrome Virus (WSSV) in Andaman and Nicobar Archipelago, India. We screened a total of 607 shrimp and 110 crab samples using a specific polymerase chain reaction, and out of them, 82 shrimps (13.5%) and 5 (4.5%) crabs were found positive for co-infection of IHHNV and WSSV. A higher rate of co-infection was observed in Penaeus monodon and Scylla serrata than other shrimp and crab species. The nucleotide sequences of IHHNV and WSSV obtained from crab in this present study exhibited very high sequence identity with their counterparts retrieved from various countries. Histopathological analysis of the infected shrimp gill sections further confirmed the eosinophilic intra-nuclear cowdry type A inclusion bodies and basophilic intra-nuclear inclusion bodies characteristics of IHHNV and WSSV infections, respectively. The present study serves as the first report on co-infection of WSSV and IHHNV in Andaman and Nicobar Archipelago, India and accentuates the critical need for continuous monitoring of wild crustaceans and appropriate biosecurity measures for brackishwater aquaculture.

scholarly journals DETEKSI WHITE SPOT SYNDROME VIRUS(WSSV) PADA UDANG WINDU (Penaeus monodon) DI BALI DAN JAWA TIMUR MENGGUNAKAN METODE POLYMERASE CHAIN REACTIOIN(PCR)

2017 ◽  
Vol 10 (1) ◽  
pp. 55
Author(s):  
Ketut Mahardika ◽  
Zafran Zafran ◽  
Lsti Koesharyani
Keyword(s):  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Polymerase Chain ◽  
White Spot Syndrome

White spot syndrome (WSS) telah ditemukan pada banyak spesies udang dan kepiting, tidak saja di Asia tetapi hampir di seluruh dunia.

scholarly journals Identification of a Shrimp E3 Ubiquitin Ligase TRIM50-Like Involved in Restricting White Spot Syndrome Virus Proliferation by Its Mediated Autophagy and Ubiquitination

2021 ◽  
Vol 12 ◽  
Author(s):  
Chao Zhao ◽  
Chao Peng ◽  
Pengfei Wang ◽  
Lulu Yan ◽  
Sigang Fan ◽  
...  
Keyword(s):  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Positive Role ◽  
Protein Levels ◽  
Mrna And Protein Expression ◽  
White Spot Syndrome ◽  
Critical Components ◽  
Trim Proteins

Most tripartite motif (TRIM) family proteins are critical components of the autophagy machinery and play important roles in host defense against viral pathogens in mammals. However, the roles of TRIM proteins in autophagy and viral infection have not been studied in lower invertebrates, especially crustaceans. In this study, we first identified a TRIM50-like gene from Penaeus monodon (designated PmTRIM50-like), which, after a white spot syndrome virus (WSSV) challenge, was significantly upregulated at the mRNA and protein levels in the intestine and hemocytes. Knockdown of PmTRIM50-like led to an increase in the WSSV quantity in shrimp, while its overexpression led to a decrease compared with the controls. Autophagy can be induced by WSSV or rapamycin challenge and has been shown to play a positive role in restricting WSSV replication in P. monodon. The mRNA and protein expression levels of PmTRIM50-like significantly increased with the enhancement of rapamycin-induced autophagy. The autophagy activity induced by WSSV or rapamycin challenge could be inhibited by silencing PmTRIM50-like in shrimp. Further studies showed that rapamycin failed to induce autophagy or inhibit WSSV replication after knockdown of PmTRIM50-like. Moreover, pull-down and in vitro ubiquitination assays demonstrated that PmTRIM50-like could interact with WSSV envelope proteins and target them for ubiquitination in vitro. Collectively, this study demonstrated that PmTRIM50-like is required for autophagy and is involved in restricting the proliferation of WSSV through its ubiquitination. This is the first study to report the role of a TRIM family protein in virus infection and host autophagy in crustaceans.

scholarly journals Evaluation of an immunodot test to manage white spot syndrome virus (WSSV) during cultivation of the giant tiger shrimp Penaeus monodon

2008 ◽  
Vol 79 ◽  
pp. 157-161 ◽  
Author(s):  
R Patil ◽  
KJ Palaksha ◽  
TM Anil ◽  
Guruchannabasavanna ◽  
P Patil ◽  
...  
Keyword(s):  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
White Spot ◽  
Tiger Shrimp ◽  
White Spot Syndrome
Sign in / Sign up

Export Citation Format

Share Document