scholarly journals The Potential of Soluble Human Leukocyte Antigen Molecules for Early Cancer Detection and Therapeutic Vaccine Design

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 775
Author(s):  
Amy L. Kessler ◽  
Marco J. Bruno ◽  
Sonja I. Buschow
Keyword(s):  
Human Leukocyte Antigen ◽  
Target Identification ◽  
Therapeutic Vaccine ◽  
Human Leukocyte ◽  
Full Potential ◽  
Leukocyte Antigen ◽  
Bodily Fluids ◽  
Soluble Hla ◽  
The Relationship ◽  
Hla Molecules

Human leukocyte antigen (HLA) molecules are essential for anti-tumor immunity, as they display tumor-derived peptides to drive tumor eradication by cytotoxic T lymphocytes. HLA molecules are primarily studied as peptide-loaded complexes on cell membranes (mHLA) and much less attention is given to their secretion as soluble HLA–peptide complexes (sHLA) into bodily fluids. Yet sHLA levels are altered in various pathologies including cancer, and are thus of high interest as biomarkers. Disconcordance in results across studies, however, hampers interpretation and generalization of the relationship between sHLA levels and cancer presence, thereby impairing its use as a biomarker. Furthermore, the question remains to what extent sHLA complexes exert immunomodulatory effects and whether shifts in sHLA levels contribute to disease or are only a consequence of disease. sHLA complexes can also bear tumor-derived peptides and recent advancements in mass spectrometry now permit closer sHLA peptide cargo analysis. sHLA peptide cargo may represent a “liquid biopsy” that could facilitate the use of sHLA for cancer diagnosis and target identification for therapeutic vaccination. This review aims to outline the contradictory and unexplored aspects of sHLA and to provide direction on how the full potential of sHLA as a quantitative and qualitative biomarker can be exploited.

Human leukocyte antigen (HLA)-G during pregnancy part II: Associations between maternal and fetal HLA-G genotypes and soluble HLA-G

2015 ◽  
Vol 76 (4) ◽  
pp. 260-271 ◽  
Author(s):  
Mette Dahl ◽  
Louise Klitkou ◽  
Ole B. Christiansen ◽  
Snezana Djurisic ◽  
Zofia Maria Piosik ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
Leukocyte Antigen ◽  
Soluble Hla

Is schizophrenia an HLA-associated disease?

1979 ◽  
Vol 9 (4) ◽  
pp. 721-728 ◽  
Author(s):  
Peter McGuffin
Keyword(s):  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
Postsynaptic Membrane ◽  
Leukocyte Antigen ◽  
Hla System ◽  
Central Neurotransmitters ◽  
Disease Subtypes ◽  
The Relationship

SYNOPSISCertain specificities of the human leukocyte antigen (HLA) system have been shown to be associated with particular diseases. A review of recent studies in schizophrenia shows inconsistent results for schizophrenia as a whole, although a significant increase in HLA A28 remains on combining the data. There are more consistent findings for disease subtypes. In particular, HLA A9 and HLA CW4 are increased in paranoid schizophrenics, while HLA Al and the A1–B8 haplotype are increased in nuclear forms. It is postulated that the relationship between the schizophrenias and certain HLA types could be due to an influence of the latter upon neuronal postsynaptic membrane sensitivity to central neurotransmitters such as dopamine.

scholarly journals The opposite effect of human leukocyte antigen genotypes in sarcoidosis and tuberculosis: a narrative review of the literature

2020 ◽  
Vol 6 (3) ◽  
pp. 00155-2020
Author(s):  
Anna Malkova ◽  
Anna Starshinova ◽  
Yulia Zinchenko ◽  
Natalia Basantsova ◽  
Vera Mayevskaya ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
Narrative Review ◽  
Autoimmune Response ◽  
Review Of The Literature ◽  
Leukocyte Antigen ◽  
Online Databases ◽  
Hla Genotypes ◽  
Common Pathogenesis ◽  
The Relationship

Sarcoidosis and tuberculosis share several similar clinical and pathogenic characteristics that make some researchers consider a common pathogenesis for these diseases. Human leukocyte antigen (HLA) genotypes are studied both in sarcoidosis and tuberculosis patients, but to our knowledge, there are no comparative studies of genetic predisposition for sarcoidosis and tuberculosis development.The aim of this review was to analyse the relationship between HLA genotypes and the development of sarcoidosis and tuberculosis. Original and review articles published in various online databases from 1960 to 2019 were studied.The search results showed opposite effects of the HLA genotypes on predisposition to sarcoidosis or tuberculosis. It was revealed that the genotypes predisposing to the development of sarcoidosis (HLA-DRB1*03/07/15) have protective properties against the development of tuberculosis. Moreover, genotypes causing the development of tuberculosis (HLA-DRB1*04) have a protective effect on the development of sarcoidosis.The results of this narrative review of the literature may allude to the existence of genetic predispositions that lead to the development of an antibacterial or autoimmune response to mycobacteria.

Human leukocyte antigen (HLA)-G during pregnancy part I: Correlations between maternal soluble HLA-G at midterm, at term, and umbilical cord blood soluble HLA-G at term

2015 ◽  
Vol 76 (4) ◽  
pp. 254-259 ◽  
Author(s):  
Louise Klitkou ◽  
Mette Dahl ◽  
Thomas Vauvert F. Hviid ◽  
Snezana Djurisic ◽  
Zofia Maria Piosik ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Leukocyte ◽  
Leukocyte Antigen ◽  
Soluble Hla

Acquisition of intact allogeneic human leukocyte antigen molecules by human dendritic cells

Blood ◽  
2000 ◽  
Vol 95 (11) ◽  
pp. 3473-3477 ◽  
Author(s):  
Vincenzo Russo ◽  
Dan Zhou ◽  
Claudia Sartirana ◽  
Patrizia Rovere ◽  
Antonello Villa ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
Surface Marker ◽  
Cell Contact ◽  
Leukocyte Antigen ◽  
Vector Coding ◽  
Intact Membrane ◽  
Human Dendritic Cells ◽  
Hla Molecules

In an attempt to transduce monocyte-derived dendritic cells (DCs) by a retroviral vector coding for a cell surface marker, we were confronted by the observation of high transfer of the surface molecule in the absence of vector proviral DNA in the treated cells. Indeed, DCs acquired the surface marker by a mechanism independent of the vector machinery, requiring cell-to-cell contact and involving transfer of lipids and a variety of intact membrane proteins. Most important, this property of DCs also includes acquisition of foreign human leukocyte antigen (HLA) molecules. Consequently, DCs become immunological hybrids as they display their own and foreign HLA molecules. The newly acquired HLA is fully functional because it allows recognition by allo-specific T lymphocytes and the binding and presentation of antigen peptides.

scholarly journals Solutions to Avoid False Positives for Rituximab in Pre-Transplant Crossmatches

Antibodies ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 41
Author(s):  
Argentina Colmenero Velazquez ◽  
Ignacio Iturrieta-Zuazo ◽  
Juan Luis Valdivieso Shephard ◽  
Marisa Di Natale ◽  
Claudia Rita ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Flow Cytometry ◽  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
False Positives ◽  
Leukocyte Antigen ◽  
Complement Dependent Cytotoxicity ◽  
Donor Cells ◽  
Anti Cd20 ◽  
Hla Molecules

Rituximab (anti-CD20) is commonly used as immunotherapy against B cells, in the context of pre-transplant crossmatches, where the presence of rituximab in the tested sera with donor cells can alter their results both by flow cytometry (FCXM) as complement-dependent cytotoxicity (CDCXM) giving rise to false positives. In the present study, we tested the use of an anti-rituximab monoclonal antibody (10C5, Abnova) as a method to avoid false positives in FCXM and CDCXM. We used the serum from ten patients who received therapy with rituximab, and the cells were incubated with sera treated or untreated with the 10C5 clone. In previous studies, attempts have been made to control these false positives through the use of pronase, although in these cases the alteration of Human Leukocyte Antigen (HLA) molecules has been found to be a limitation. As an alternative, we performed an assay to exclude false positives by a pre-incubation with anti-rituximab antibody (10C5) in 1:5 proportion avoiding the misinterpretation of crossmatches, particularly in patients with specific donor antibodies (DSA) without affecting the HLA molecules.

scholarly journals The relationship between human leukocyte antigen-cw6 allele and psoriasis vulgaris

2019 ◽  
Author(s):  
Sri Lestari KS ◽  
Eryati Darwin ◽  
Tjut Nurul Alam Jacoeb ◽  
Djong Hon Tjong
Keyword(s):  
Human Leukocyte Antigen ◽  
Psoriasis Vulgaris ◽  
Human Leukocyte ◽  
Control Group ◽  
Cross Sectional ◽  
Leukocyte Antigen ◽  
Nucleotide Base ◽  
Dermatological Examination ◽  
Observation Method ◽  
The Relationship

Psoriasis vulgaris is chronic skin disease that is linked to genetics and immune system. The most important predisposing genetic factor is human leukocyte antigen (HLA). This study was performed to determine the relationship between HLA-Cw6 allele and psoriasis vulgaris and the changes of nucleotide base squences, using observation method with a cross sectional comparative study. Samples were selected using consecutive sampling of 30 patients with psoriasis vulgaris attending the Dermatology and STD polyclinic at DR. M. Djamil Hospital. 30 healthy volunteers were selected as controls. The subjects’ medical history was recorded followed by a dermatological examination, collection of samples, DNA isolation, then primers were designed for HLA-Cw6 allele, genes were sequencing and finally analyzed using PCR-SSP. The results were 20% of patients with psoriasis vulgaris carried HLA-Cw6 allele, while it was absent in the control group. This difference is statistically significant at the 5% level (p = 0.024). We found the changes of nucleotide base formations of HLA-Cw6. In conclusion, based on these observations, presence of the HLA-Cw6 allele is an important genetic risk factor for developing psoriasis vulgaris.

scholarly journals Soluble human leukocyte antigen-G evaluation in pregnant women with gestational diabetes mellitus

2020 ◽  
Vol 32 (1) ◽  
Author(s):  
Muhamad R. Abdel Hameed ◽  
Osama Ahmed Ibrahiem ◽  
Entsar Hamed Ahmed ◽  
Paula Rofaeel Sedky ◽  
Naglaa Mohamed M. A. Mousa
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Human Leukocyte Antigen ◽  
Pregnant Women ◽  
Human Leukocyte ◽  
Normal Pregnancy ◽  
Human Major Histocompatibility Complex ◽  
Leukocyte Antigen ◽  
Soluble Hla

Abstract Background Gestational diabetes mellitus is any degree of glucose intolerance with onset or first recognition occurring late in second trimester and third trimester of pregnancy. It constitutes a greater impact on diabetes epidemic as it carries a major risk for developing type 2 diabetes mellitus to the mother and her fetus later in life. human leukocyte antigen (HLA)-G is a class Ib gene presents in the human major histocompatibility complex (MHC). HLA-G has an important role for mother and fetus tolerance during pregnancy, also in the pancreatic islet cells protection. This is a case-control study, measuring serum HLA-G levels by ELISA in 60 pregnant women with gestational diabetes compared with 36 normal pregnant women. Results HLA-G levels were significantly high in pregnant women with gestational diabetes mellitus (GDM) in contrast to women with normal pregnancy (P = 0.001). Conclusion Women with GDM had significantly higher levels of soluble HLA-G than women without GDM, suggesting that HLA-G molecule is among the factors for regulation and control of the immune response and the induction of tolerance. Soluble HLA-G could be considered an important follow-up investigation for all pregnant primary health care for early detection of gestational diabetes.

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