scholarly journals SARS-CoV-2 Production in a Scalable High Cell Density Bioreactor

Vaccines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 706
Author(s):  
Anna Offersgaard ◽  
Carlos Rene Duarte Hernandez ◽  
Anne Finne Pihl ◽  
Rui Costa ◽  
Nandini Prabhakar Venkatesan ◽  
...  
Keyword(s):  
Virus Production ◽  
Packed Bed ◽  
Cell Number ◽  
Packed Bed Bioreactor ◽  
Infectious Dose ◽  
Working Volume ◽  
Production Temperature ◽  
Infectivity Titer ◽  
Tissue Culture Infectious Dose ◽  
Animal Component

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has demonstrated the value of pursuing different vaccine strategies. Vaccines based on whole viruses, a widely used vaccine technology, depend on efficient virus production. This study aimed to establish SARS-CoV-2 production in the scalable packed-bed CelCradleTM 500-AP bioreactor. CelCradleTM 500-AP bottles with 0.5 L working volume and 5.5 g BioNOC™ II carriers were seeded with 1.5 × 108 Vero (WHO) cells, approved for vaccine production, in animal component-free medium and infected at a multiplicity of infection of 0.006 at a total cell number of 2.2–2.5 × 109 cells/bottle seven days post cell seeding. Among several tested conditions, two harvests per day and a virus production temperature of 33 °C resulted in the highest virus yield with a peak SARS-CoV-2 infectivity titer of 7.3 log10 50% tissue culture infectious dose (TCID50)/mL at 72 h post-infection. Six harvests had titers of ≥6.5 log10 TCID50/mL, and a total of 10.5 log10 TCID50 were produced in ~5 L. While trypsin was reported to enhance virus spread in cell culture, addition of 0.5% recombinant trypsin after infection did not improve virus yields. Overall, we demonstrated successful animal component-free production of SARS-CoV-2 in well-characterized Vero (WHO) cells in a scalable packed-bed bioreactor.

Three-dimensional CFD simulation of anaerobic reactions in a continuous packed-bed bioreactor

2021 ◽  
Author(s):  
Sasan Zarei ◽  
Seyyed Mohammad Mousavi ◽  
Teimour Amani ◽  
Mehrdad Khamforoush ◽  
Arezou Jafari
Keyword(s):  
Cfd Simulation ◽  
Three Dimensional ◽  
Packed Bed ◽  
Packed Bed Bioreactor

Toxicity and recalcitrant compound removal from bleaching pulp plant effluents by an integrated system: anaerobic packed-bed bioreactor and ozone

2010 ◽  
Vol 61 (1) ◽  
pp. 199-205 ◽  
Author(s):  
T. R. Chaparro ◽  
C. M. Botta ◽  
E. C. Pires
Keyword(s):  
Pulp Mill ◽  
Packed Bed ◽  
Ultraviolet Absorption ◽  
Integrated System ◽  
Packed Bed Bioreactor ◽  
Cellulose Pulp ◽  
Adsorbable Organic Halides ◽  
Organic Halides ◽  
Before And After ◽  
Absorption Measurements

Effluents originated in cellulose pulp manufacturing processes are usually toxic and recalcitrant, specially the bleaching effluents, which exhibit high contents of aromatic compounds (e.g. residual lignin derivates). Although biological processes are normally used, their efficiency for the removal of toxic lignin derivates is low. The toxicity and recalcitrance of a bleached Kraft pulp mill were assessed through bioassays and ultraviolet absorption measurements, i.e. acid soluble lignin (ASL), UV280, and specific ultraviolet absorption (SUVA), before and after treatment by an integrated system comprised of an anaerobic packed-bed bioreactor and oxidation step with ozone. Furthermore, adsorbable organic halides (AOX) were measured. The results demonstrated not only that the toxic recalcitrant compounds can be removed successfully using integrated system, but also the ultraviolet absorption measurements can be an interesting control-parameter in a wastewater treatment.

Production of HIV-1 gp120 in Packed-Bed Bioreactor Using the Vaccinia Virus/T7 Expression System

2000 ◽  
Vol 16 (5) ◽  
pp. 744-750 ◽  
Author(s):  
Y.-C. Hu ◽  
J. Kaufman ◽  
M.W. Cho ◽  
H. Golding ◽  
J. Shiloach
Keyword(s):  
Vaccinia Virus ◽  
Expression System ◽  
Packed Bed ◽  
Packed Bed Bioreactor ◽  
T7 Expression System ◽  
Hiv 1

scholarly journals β-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing

Biomolecules ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 227 ◽  
Author(s):  
Camila Favaro ◽  
Ilton Baraldi ◽  
Fernanda Casciatori ◽  
Cristiane Farinas
Keyword(s):  
Hydrolytic Enzymes ◽  
Packed Bed ◽  
Added Value ◽  
Industrial Processes ◽  
Enzymatic Extract ◽  
Packed Bed Bioreactor ◽  
Industry Waste ◽  
Hydrolysis Of ◽  
Soluble Coffee

Soluble coffee offers the combined benefits of high added value and practicality for its consumers. The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts. This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing. Different substrate compositions were studied, an ideal microorganism was selected, and the fermentation conditions were optimized. Cultivations for enzymes production were carried out in flasks and in a packed-bed bioreactor. Higher enzyme yield was achieved in the bioreactor, due to better aeration of the substrate. The best β-mannanase production results were found for a substrate composed of a mixture of coffee waste and wheat bran (1:1 w/w), using Aspergillus niger F12. The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee. The addition of a commercial cellulase cocktail to the crude extract increased the hydrolysis yield by 56%. The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.

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