scholarly journals Heterologous ChAdOx1 nCoV-19/BNT162b2 Prime-Boost Vaccination Induces Strong Humoral Responses among Health Care Workers

Vaccines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 857
Author(s):  
Louise Benning ◽  
Maximilian Töllner ◽  
Asa Hidmark ◽  
Matthias Schaier ◽  
Christian Nusshag ◽  
...  
Keyword(s):  
Health Care ◽  
Health Care Workers ◽  
Neutralizing Antibodies ◽  
Age Groups ◽  
Humoral Response ◽  
University Hospital ◽  
Boost Vaccination ◽  
Care Workers ◽  
Igg Index ◽  
Humoral Responses

Despite limited data on safety and immunogenicity, heterologous prime-boost vaccination is currently recommended for individuals with ChAdOx1 nCoV-19 prime immunization in certain age groups. In this prospective, single-center study we included 166 health care workers from Heidelberg University Hospital who received either heterologous ChAdOx1 nCoV-19/BNT162b2, homologous BNT162b2 or homologous ChAdOx1 nCoV-19 vaccination between December 2020 and May 2021. We measured anti-S1 IgG, SARS-CoV-2 specific neutralizing antibodies, and antibodies against different SARS-CoV-2 fragments 0–3 days before and 19–21 days after boost vaccination. Before boost, 55/70 (79%) ChAdOx1 nCoV-19-primed compared with 44/45 (98%) BNT162b2-primed individuals showed positive anti-S1 IgG with a median (IQR) anti-S1 IgG index of 1.95 (1.05–2.99) compared to 9.38 (6.26–17.12). SARS-CoV-2 neutralizing antibodies exceeded the threshold in 24/70 (34%) of ChAdOx1 nCoV-19-primed and 43/45 (96%) of BNT162b2-primed individuals. After boosting dose, median (IQR) anti-S1 IgG index in heterologous ChAdOx1 nCoV-19/BNT162b2 vaccinees was 116.2 (61.84–170), compared to 13.09 (7.03–29.02) in homologous ChAdOx1 nCoV-19 and 145.5 (100–291.1) in homologous BNT162b2 vaccinees. All boosted vaccinees exceeded the threshold for neutralization, irrespective of their vaccination scheme. Vaccination was well-tolerated overall. We show that heterologous ChAdOx1 nCoV-19/BNT162b2 vaccination is safe and induces a strong and broad humoral response in healthy individuals.

scholarly journals SARS-COV-2 Neutralizing Activity in Serum Collected from Recovered and Vaccinated Health Care Workers

2021 ◽  
Author(s):  
Lina MOUNA ◽  
Mehdi Razazian ◽  
Sandra Duquesne ◽  
Roque-Afonso Roque-Afonso ◽  
Christelle Vauloup-Fellous
Keyword(s):  
Health Care ◽  
Health Care Workers ◽  
Neutralizing Antibodies ◽  
Humoral Response ◽  
High Specificity ◽  
Cross Reactivity ◽  
Optimal Thresholds ◽  
Care Workers ◽  
Time Specific ◽  
Roche Diagnostics

Abstract Vaccination against the ongoing COVID-19 is the key point in fight against the pandemic. The Spike (S) glycoprotein of SARS-CoV-2 is the major target of the neutralizing humoral response. We evaluated analytical and clinical performances of a surrogate virus neutralization test (sVNT) (iFlash-2019-nCoV Nab assay, Ylho, China) compared to the conventional neutralization tests (cVNT) and anti-S eCLIA assays (Roche Diagnostics, Switzerland) in recovered and/or vaccinated health care workers. Our results indicate that sVNT displayed high specificity and no cross reactivity. Both Roche and iFlash immunoassays were good in identifying cVNT serum dilution > 1:16. Optimal thresholds in identifying cVNT titers ≥ 1:16, were 74.5 U/ml and 49.4 IU/ml for anti-S eCLIA and sVNT, respectively. Our data show that Nab neutralizing antibodies titers depend on individuals and may abate over time. Specific assays such as sVNT may be a reliable complementary tool to routine anti-S serology assays.

Corona Virus (COVID-19) Awareness Assessment - A Survey Study Amongst the Indian Population

2020 ◽  
Author(s):  
Gabriela Fernandes
Keyword(s):  
Health Care ◽  
Social Media ◽  
Health Care Workers ◽  
Indian Population ◽  
Age Groups ◽  
Survey Study ◽  
Social Distancing ◽  
Corona Virus ◽  
Care Workers ◽  
Amount Of Knowledge

Aim: The aim of this survey study was to assess the level of awareness amongst Indian population regarding the COVID-19. Method: A survey was conducted amongst 745 individuals to assess their level of awareness regarding COVID-19 and steps to be taken for its prevention. Result: The results revealed that a considerable percentage of individuals learned about the pandemic through social media and news and were aware of the mode of spread of the virus and also steps to be taken to prevent it from spreading. But considerable percentage of people was also not fully aware regarding the age groups this virus will be affecting. Conclusion: Upon understanding the percentage of people not aware about the age groups this virus will be affecting, keeping in mind good amount of knowledge amongst individuals about maintaining hygiene and social distancing, this survey would help the health care workers to create awareness regarding the effect of this virus on different age groups to help prevent carelessness amongst youth in following the regime.

scholarly journals Adherence to personal protective equipment use among healthcare workers caring for confirmed COVID-19 and alleged non-COVID-19 patients

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Meike M. Neuwirth ◽  
Frauke Mattner ◽  
Robin Otchwemah
Keyword(s):  
Health Care ◽  
Health Care Workers ◽  
Personal Protective Equipment ◽  
Healthcare Workers ◽  
University Hospital ◽  
Protective Equipment ◽  
Care Workers

AbstractAdherence observations of health care workers (HCW) revealed deficiencies in the use of recommended personal protective equipment (PPE) among HCW caring in COVID-19 and non-COVID-19 wards during the first period of the SARS-CoV-2 pandemic in a university hospital in Germany. The adherence to wearing surgical face or FFP2-masks and disinfecting hands prior to donning and after doffing the PPE was significantly higher in COVID-19 wards However, there was no total adherence of 100% in COVID-19 wards.

scholarly journals Prevalence and risk factors for Staphylococcus aureus in health care workers at a University Hospital of Recife-PE

2008 ◽  
Vol 12 (6) ◽  
pp. 504-508 ◽  
Author(s):  
Eduardo Caetano Brandão Ferreira da Silva ◽  
Maria das Graças C. Antas ◽  
Armando Monteiro B. Neto ◽  
Marcelle Aquino Rabelo ◽  
Fábio Lopes de Melo ◽  
...  
Keyword(s):  
Risk Factors ◽  
Health Care ◽  
Staphylococcus Aureus ◽  
Health Care Workers ◽  
University Hospital ◽  
Care Workers

scholarly journals SARS-CoV-2–Specific Neutralizing Antibody Responses in Norwegian Health Care Workers After the First Wave of COVID-19 Pandemic: A Prospective Cohort Study

2020 ◽  
Author(s):  
Mai-Chi Trieu ◽  
Amit Bansal ◽  
Anders Madsen ◽  
Fan Zhou ◽  
Marianne Sævik ◽  
...  
Keyword(s):  
Health Care ◽  
Cohort Study ◽  
Prospective Cohort Study ◽  
Health Care Workers ◽  
Prospective Cohort ◽  
Neutralizing Antibodies ◽  
Rt Pcr ◽  
Patient Exposure ◽  
Specific Antibodies ◽  
Care Workers

Abstract Background During the coronavirus disease 2019 (COVID-19) pandemic, many countries experienced infection in health care workers (HCW) due to overburdened health care systems. Whether infected HCW acquire protective immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is unclear. Methods In a Norwegian prospective cohort study, we enrolled 607 HCW before and after the first COVID-19 wave. Exposure history, COVID-19–like symptoms, and serum samples were collected. SARS-CoV-2–specific antibodies were characterized by spike-protein IgG/IgM/IgA enzyme-linked immunosorbent and live-virus neutralization assays. Results Spike-specific IgG/IgM/IgA antibodies increased after the first wave in HCW with, but not in HCW without, COVID-19 patient exposure. Thirty-two HCW (5.3%) had spike-specific antibodies (11 seroconverted with ≥4-fold increase, 21 were seropositive at baseline). Neutralizing antibodies were found in 11 HCW that seroconverted, of whom 4 (36.4%) were asymptomatic. Ninety-seven HCW were tested by reverse transcriptase polymerase chain reaction (RT-PCR) during follow-up; 8 were positive (7 seroconverted, 1 had undetectable antibodies). Conclusions We found increases in SARS-CoV-2 neutralizing antibodies in infected HCW, especially after COVID-19 patient exposure. Our data show a low number of SARS-CoV-2–seropositive HCW in a low-prevalence setting; however, the proportion of seropositivity was higher than RT-PCR positivity, highlighting the importance of antibody testing.

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