scholarly journals Immunization with Brucella abortus S19Δper Conferred Protection in Water Buffaloes against Virulent Challenge with B. abortus Strain S544

Vaccines ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1423
Author(s):  
Pallab Chaudhuri ◽  
Mani Saminathan ◽  
Syed Atif Ali ◽  
Gurpreet Kaur ◽  
Shiv Varan Singh ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Protective Efficacy ◽  
Virulent Strain ◽  
Bovine Brucellosis ◽  
Buffalo Calves ◽  
Post Vaccination ◽  
Colony Forming Units ◽  
Gross Lesions ◽  
Virulent Challenge ◽  
Gene Deletion Mutant

Vaccination of cattle and buffaloes with Brucella abortus strain 19 has been the mainstay for control of bovine brucellosis. However, vaccination with S19 suffers major drawbacks in terms of its safety and interference with serodiagnosis of clinical infection. Brucella abortus S19∆per, a perosamine synthetase wbkB gene deletion mutant, overcomes the drawbacks of the S19 vaccine strain. The present study aimed to evaluate the potential of Brucella abortus S19Δper vaccine candidate in the natural host, buffaloes. Safety of S19∆per, for animals use, was assessed in guinea pigs. Protective efficacy of vaccine was assessed in buffaloes by immunizing with normal dose (4 × 1010 colony forming units (CFU)/animal) and reduced dose (2 × 109 CFU/animal) of S19Δper and challenged with virulent strain of B. abortus S544 on 300 days post immunization. Bacterial persistency of S19∆per was assessed in buffalo calves after 42 days of inoculation. Different serological, biochemical and pathological studies were performed to evaluate the S19∆per vaccine. The S19Δper immunized animals showed significantly low levels of anti-lipopolysaccharides (LPS) antibodies. All the immunized animals were protected against challenge infection with B. abortus S544. Sera from the majority of S19Δper immunized buffalo calves showed moderate to weak agglutination to RBPT antigen and thereby, could apparently be differentiated from S19 vaccinated and clinically-infected animals. The S19Δper was more sensitive to buffalo serum complement mediated lysis than its parent strain, S19. Animals culled at 6-weeks-post vaccination showed no gross lesions in organs and there was comparatively lower burden of infection in the lymph nodes of S19Δper immunized animals. With attributes of higher safety, strong protective efficacy and potential of differentiating infected from vaccinated animals (DIVA), S19Δper would be a prospective alternate to conventional S19 vaccines for control of bovine brucellosis as proven in buffaloes.

scholarly journals Protective Immunity Elicited by a Divalent DNA Vaccine Encoding Both the L7/L12 and Omp16 Genes of Brucella abortus in BALB/c Mice

2006 ◽  
Vol 74 (5) ◽  
pp. 2734-2741 ◽  
Author(s):  
Deyan Luo ◽  
Bing Ni ◽  
Peng Li ◽  
Wei Shi ◽  
Songle Zhang ◽  
...  
Keyword(s):  
Immune Response ◽  
Dna Vaccine ◽  
Brucella Abortus ◽  
Protective Efficacy ◽  
Virulent Strain ◽  
T Helper 1 ◽  
Genetic Vaccine ◽  
Cellular Immune

ABSTRACT This study was designed to evaluate the immunogenicity and the protective efficacy of a divalent fusion DNA vaccine encoding both the Brucella abortus L7/L12 protein (ribosomal protein) and Omp16 protein (outer membrane lipoprotein), designated pcDNA3.1-L7/L12-Omp16. Intramuscular injection of this divalent DNA vaccine into BALB/c mice elicited markedly both humoral and cellular immune responses. The specific antibodies exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. In addition, the dual-gene DNA vaccine elicited a strong T-cell proliferative response and induced a large amount of gamma interferon-producing T cells upon restimulation in vitro with recombinant fusion protein L7/L12-Omp16, suggesting the induction of a typical T-helper-1-dominated immune response in vivo. This divalent DNA vaccine could also induce a significant level of protection against challenge with the virulent strain B. abortus 544 in BALB/c mice. Furthermore, the protection level induced by the divalent DNA vaccine was significantly higher than that induced by the univalent DNA vaccines pcDNA3.1-L7/L12 or pcDNA3.1-Omp16. Taken together, the results of this study verify for the first time that the Omp16 gene can be a candidate target for a DNA vaccine against brucellosis. Additionally, a divalent genetic vaccine based on the L7/L12 and Omp16 genes can elicit a stronger cellular immune response and better immunoprotection than the relevant univalent vaccines can.

scholarly journals Efficacy of Brucella abortus S19 and RB51 vaccine strains: a systematic review and meta-analysis

2021 ◽  
Author(s):  
Elaine Dorneles ◽  
Marina Martins de Oliveira ◽  
Carine Pereira ◽  
Izabela Oliveira ◽  
Jacques Godfroid ◽  
...  
Keyword(s):  
Systematic Review ◽  
Vaccine Strain ◽  
Brucella Abortus ◽  
Meta Analysis ◽  
Significant Protection ◽  
Bovine Brucellosis ◽  
Colony Forming Units ◽  
Protection Rate ◽  
Protection Against Infection ◽  
S19 Vaccine

This systematic review and meta-analysis aimed to recalculate the efficacy of these two vaccine strains, and to discuss the main variables associated with controlled trials to evaluate bovine brucellosis vaccines efficacy. The most used vaccine strain was S19, at the dose of 10 colony forming units (CFU), followed by the vaccine strain RB51 at 10 CFU. The most used challenge strain was B. abortus 2308, at the dose of 10 CFU by intraconjunctival route. For the meta-analysis, trials were grouped according to the vaccine strain and dose to recalculate protection against abortion (four groups) or infection (five groups), using pooled risk ratio (RR) and vaccine efficacy (VE). For protection against abortion (n = 15 trials), S19 vaccine at 10 CFU exhibited the highest protection rate (RR = 0.25, 95% CI: 0.12 to 0.52; VE = 75.09%, 95% CI: 48.08 – 88.05), followed by RB51 10 (RR = 0.31, 95% CI: 0.16 to 0.61; VE = 69.25%, 95% CI: 39.48 – 84.38). For protection against infection (n = 23 trials), only two subgroups exhibited significant protection: S19 at 10 CFU (RR = 0.28, 95% CI: 0.14 to 0.55; VE = 72.03%, 95% CI: 57.70 – 81.50) and RB51 at 10 CFU dose (RR = 0.43, 95% CI: 0.22 to 0.84; VE = 57.05%, 95% CI: 30.90 – 73.30). In conclusion, our results suggest that the dose of 10 CFU for S19 and 10 CFU for RB51 are the most suitable for the prevention of abortion and infection caused by B. abortus.

scholarly journals Effects of live Brucella abortus strain 19 vaccine on reindeer

Rangifer ◽  
1989 ◽  
Vol 9 (2) ◽  
pp. 47 ◽  
Author(s):  
Robert A. Dieterich ◽  
Jamie K. Morton
Keyword(s):  
Brucella Abortus ◽  
Rangifer Tarandus ◽  
Control Program ◽  
Pregnant Female ◽  
Post Vaccination ◽  
Reduced Dose ◽  
Colony Forming Units ◽  
Challenge Experiment ◽  
Carpal Joint ◽  
The Right

Twenty female and seven male reindeer (Rangifer tarandus) were vaccinated subcutaneously in the right shoulder with a 1-ml dose of approximately 1.2 x 108 colony forming units of Brucella abortus strain 19, the standard reduced dose for cattle. An additional three females and one male served as non-vaccinated sentinels. Brucella abortus strain 19 was isolated from two of three fetuses aborted by vaccinated females during the first of two fawning seasons. Serologic titers to brucellosis in the vaccinates peaked by 46 days post-vaccination. Shedding of B. abortus strain 19 by vaccinated animals was indicated by seroconversion of all four sentinels. Titers in the sentinels were low and sporadic. Brucella abortus strain 19 was isolated from the tissues and fetus of a pregnant female 51 days post-vaccination and from the carpal joint of another female 7 months post-vaccination. Based on these results and a previous challenge experiment, it was concluded that Brucella abortus strain 19 is not a suitable vaccine to use in a brucellosis control program in reindeer.

scholarly journals Use ofS-[2,3-Bispalmitoyiloxy-(2R)-Propyl]-R-Cysteinyl-Amido-Monomethoxy Polyethylene Glycol as an Adjuvant Improved Protective Immunity Associated with a DNA Vaccine Encoding Cu,Zn Superoxide Dismutase of Brucella abortus in Mice

2014 ◽  
Vol 21 (11) ◽  
pp. 1474-1480 ◽  
Author(s):  
Angello Retamal-Díaz ◽  
Roberto Riquelme-Neira ◽  
Darwin Sáez ◽  
Alejandra Rivera ◽  
Pablo Fernández ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Polyethylene Glycol ◽  
Dna Vaccine ◽  
Brucella Abortus ◽  
Protective Efficacy ◽  
Virulent Strain ◽  
Vaccination Strategy ◽  
Secretory Iga ◽  
Content Type ◽  
Humoral Responses

ABSTRACTThis study was conducted to evaluate the immunogenicity and protective efficacy of a DNA vaccine encodingBrucella abortusCu,Zn superoxide dismutase (SOD) using the Toll-like receptor 2/6 agonistS-[2,3-bispalmitoyiloxy-(2R)-propyl]-R-cysteinyl-amido-monomethoxy polyethylene glycol (BPPcysMPEG) as an adjuvant. Intranasal coadministration of BPPcysMPEG with a plasmid carrying the SOD-encoding gene (pcDNA-SOD) into BALB/c mice elicited antigen-specific humoral and cellular immune responses. Humoral responses were characterized by the stimulation of IgG2a and IgG1 and by the presence of SOD-specific secretory IgA in nasal and bronchoalveolar lavage fluids. Furthermore, T-cell proliferative responses and increased production of gamma interferon were also observed upon splenocyte restimulation with recombinant SOD. Cytotoxic responses were also stimulated, as demonstrated by the lysis of RB51-SOD-infected J774.A1 macrophages by cells recovered from immunized mice. The pcDNA-SOD/BPPcysMPEG formulation induced improved protection against challenge with the virulent strainB. abortus2308 in BALB/c mice over that provided by pcDNA-SOD, suggesting the potential of this vaccination strategy againstBrucellainfection.

scholarly journals A DNA Vaccine Encoding Cu,Zn Superoxide Dismutase of Brucella abortus Induces Protective Immunity in BALB/c Mice

2003 ◽  
Vol 71 (9) ◽  
pp. 4857-4861 ◽  
Author(s):  
Angel A. Oñate ◽  
Sandra Céspedes ◽  
Alex Cabrera ◽  
Rodolfo Rivers ◽  
Andrés González ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Dna Vaccine ◽  
Brucella Abortus ◽  
Protective Efficacy ◽  
Virulent Strain ◽  
Interleukin 10 ◽  
T Helper 1 ◽  
Cellular Immune Responses ◽  
The One ◽  
Cellular Immune

ABSTRACT This study was conducted to evaluate the immunogenicity and protective efficacy of a DNA vaccine encoding Brucella abortus Cu,Zn superoxide dismutase (SOD). Intramuscular injection of plasmid DNA carrying the SOD gene (pcDNA-SOD) into BALB/c mice elicited both humoral and cellular immune responses. Animals injected with pcDNA-SOD developed SOD-specific antibodies which exhibited a dominance of immunoglobulin G2a (IgG2a) over IgG1. In addition, the DNA vaccine elicited a T-cell-proliferative response and also induced the production of gamma interferon, but not interleukin-10 (IL-10) or IL-4, upon restimulation with either recombinant SOD or crude Brucella protein, suggesting the induction of a typical T-helper-1-dominated immune response in mice. The pcDNA-SOD (but not the control vector) induced a strong, significant level of protection in BALB/c mice against challenge with B. abortus virulent strain 2308; the level of protection was similar to the one induced by B. abortus vaccine strain RB51. Altogether, these data suggest that pcDNA-SOD is a good candidate for use in future studies of vaccination against brucellosis.

scholarly journals Bovine brucellosis in slaughterhouses controlled by Federal and Municipal Inspection Services in the state of Maranhão, Brazil

2019 ◽  
Vol 86 ◽  
Author(s):  
Anna Karoline Amaral Sousa ◽  
Bruno Raphael Ribeiro Guimarães ◽  
Priscila Alencar Beserra ◽  
Danilo Cutrim Bezerra ◽  
Ferdinan de Almeida Melo ◽  
...  
Keyword(s):  
Risk Factors ◽  
Brucella Abortus ◽  
Bovine Brucellosis ◽  
Blood Samples ◽  
Male And Female ◽  
Specific Antibodies ◽  
Laboratory Results ◽  
Inspection Service ◽  
Serum Agglutination ◽  
Do So

ABSTRACT: The aims of the current study were to investigate the prevalence of bovine brucellosis, to correlate laboratory results of serum reactive samples to the serology of animals presenting serous pouches, and to identify possible risk factors for the development of the disease. In order to do so, 1,265 bovine blood samples were collected from male and female animals bred in different counties in Maranhão state, Brazil, and in other regions of the country. The animals were slaughtered in two slaughterhouses controlled by the Federal Inspection Service (FIS), and in two others controlled by the Municipal Inspection Service (MIS), of regions such as Açailândia and Imperatriz. Samples were tested for specific antibodies by using buffered acidified antigen (BAA) and 2-mercaptoethanol in combination with slow serum agglutination (2-ME + SSA). Additionally, an epidemiological questionnaire was applied to 100 owners, who provided animals to the slaughterhouses, to enable investigating the risk factors for bovine brucellosis. Fifteen serous pouches of animals presenting this lesion were also collected at slaughter time. Thirty-nine out of the analyzed samples were reacted to BAA, whereas 15 reacted to the 2-ME + SSA test; only one male was reagent and it resulted in 1.19% prevalence. One out of the 15 collected serous pouches reacted to the 2-ME + SSA test. The risk factors identified in this study were: the incidence of miscarriages, the sale of animals without previous examination, and the failure in testing the animals before introducing them in herds and before slaughter. It was possible to conclude that the animals slaughtered in the herein evaluated slaughterhouses were infected with Brucella abortus, as well as that this infection prevailed in females.

Evaluation of the combined use of the recombinant Brucella abortus Omp10, Omp19 and Omp28 proteins for the clinical diagnosis of bovine brucellosis

2015 ◽  
Vol 83-84 ◽  
pp. 41-46 ◽  
Author(s):  
Hannah Leah Tadeja Simborio ◽  
Jin Ju Lee ◽  
Alisha Wehdnesday Bernardo Reyes ◽  
Huynh Tan Hop ◽  
Lauren Togonon Arayan ◽  
...  
Keyword(s):  
Clinical Diagnosis ◽  
Brucella Abortus ◽  
Bovine Brucellosis ◽  
Combined Use

scholarly journals Immunological response to Brucella abortus strain 19 vaccination of cattle in a communal area in South Africa

2018 ◽  
Vol 89 ◽  
Author(s):  
Gregory J.G. Simpson ◽  
Tanguy Marcotty ◽  
Elodie Rouille ◽  
Abel Chilundo ◽  
Jean-Jacques Letteson ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Immunological Response ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Dose ◽  
Public Health Importance ◽  
Middle Income ◽  
Middle Income Countries ◽  
Antibody Persistence ◽  
Colony Forming Units ◽  
Communal Area

Brucellosis is of worldwide economic and public health importance. Heifer vaccination with live attenuated Brucella abortus strain 19 (S19) is the cornerstone of control in low- and middle-income countries. Antibody persistence induced by S19 is directly correlated with the number of colony-forming units (CFU) per dose. There are two vaccination methods: a ‘high’ dose (5–8 × 1010 CFU) subcutaneously injected or one or two ‘low’ doses (5 × 109 CFU) through the conjunctival route. This study aimed to evaluate serological reactions to the ‘high’ dose and possible implications of the serological findings on disease control. This study included 58 female cases, vaccinated at Day 0, and 29 male controls. Serum was drawn repeatedly and tested for Brucella antibodies using the Rose Bengal Test (RBT) and an indirect enzyme-linked immunosorbent assay (iELISA). The cases showed a rapid antibody response with peak RBT positivity (98%) at 2 weeks and iELISA (95%) at 8 weeks, then decreased in an inverse logistic curve to 14% RBT and 32% iELISA positive at 59 weeks and at 4.5 years 57% (4/7 cases) demonstrated a persistent immune response (RBT, iELISA or Brucellin skin test) to Brucella spp. Our study is the first of its kind documenting the persistence of antibodies in an African communal farming setting for over a year to years after ‘high’ dose S19 vaccination, which can be difficult to differentiate from a response to infection with wild-type B. abortus. A recommendation could be using a ‘low’ dose or different route of vaccination.

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