scholarly journals METHODS FOR STUDYING AND ASSESSING CYTOGENETIC CHANGES IN BONE MARROW CELLS OF LABORATORY ANIMALS RECEIVING GENE-MODIFIED

2021 ◽  
Vol 58 (2) ◽  
pp. 4988-4995
Author(s):  
N.A.Nuraliyev, A.Kh.Allanazarov, M.S.Gildiyeva, D.R.Sobirova
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Normal Karyotype ◽  
Laboratory Animals ◽  
Control Group ◽  
Red Bone Marrow ◽  
Comparative Aspect ◽  
Mitotic Pathology ◽  
Cytogenetic Changes ◽  
Marrow Cells

The aim was to study cytogenetic changes in bone marrow cells of laboratory animals that received and did not receive a genetically modified (GM) product (GM-soy) in a comparative aspect. It was found that in the group of white outbred rats that received GM-soy, cytogenetic changes were found in the cells of the red bone marrow, expressed in partial inhibition of cell proliferation due to the cytotoxic effect. They also had polyploidy (5.35%) and aneuploidy (5.35%) in 10.7% of cases. In addition, this group contained cells with mitotic pathology - chromosome scattering, chromosome pulverization, impaired spiralization and despiralization of chromosomes, premature spiralization of chromosomes, delayed mitosis at the prophase stage. In the group of animals that did not receive soy with and without GM in the cells, there were metaphase plates with a normal karyotype. The mitotic activity of bone marrow cells in the control group was higher (MI - 9%) than in the first (MI - 3%) and the second group (MI - 5%).  

Morphofunctional features of proliferating cells exposed to PSMA peptide

2021 ◽  
Author(s):  
N.A. Mikheeva ◽  
E.P. Drozhdina ◽  
N.A. Kurnosova
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Laboratory Animals ◽  
Laboratory Mice ◽  
Proliferating Cells ◽  
Red Bone Marrow ◽  
Toxic Damage ◽  
Dividing Cells ◽  
Marrow Cells

The effect of the synthetic PSMA peptide on dividing cells of laboratory animals was studied. The experiment was carried out on male white laboratory mice of the BALB/c-line. The toxic effect of PSMA peptidi was evaluated at therapeutic (1.4 μg / kg of animal weight or 0.04 μg / animal) and subtoxic (140 μg / kg of animal weight or 4.0 μg / animal) doses. The cytotoxic effect of PSMA peptide on red bone marrow cells and cambial intestinal cells of the of laboratory mice was determined. A decrease in the proliferative activity of the colon crypt cells was revealed upon administration of a subtoxic dose of the PSMA peptide and there were no signs of toxic damage to the red bone marrow cells of animals. Key words: toxicity, proliferation, synthetic peptides, mitotic index, micronucleus test.

Evaluation of genotoxicity of pan masala employing chromosomal aberration and micronucleus assay in bone marrow cells of the mice

2009 ◽  
Vol 25 (7) ◽  
pp. 467-471 ◽  
Author(s):  
BN Mojidra ◽  
K. Archana ◽  
AK Gautam ◽  
Y. Verma ◽  
BC Lakkad ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Chromosome Aberration ◽  
Chromosomal Aberration ◽  
Bone Marrow Cells ◽  
Micronucleus Assay ◽  
Control Group ◽  
Genotoxic Potential ◽  
Polychromatic Erythrocytes ◽  
Significant Difference ◽  
Marrow Cells

Pan masala is commonly consumed in south-east Asian and other oriental countries as an alternate of tobacco chewing and smoking. Genotoxic potential of pan masala (pan masala plain and pan masala with tobacco known as gutkha) was evaluated employing chromosome aberration (CA) and micronucleus (MN) assay in vivo. Animals were exposed to three different doses (0.5%, 1.5% and 3%) of pan masala plain (PMP) and gutkha (PMT) through feed for a period of 6 months and micronucleus and chromosomal aberrations were studied in the bone marrow cells. Induction of mean micronuclei in polychromatic erythrocytes (MNPCE) and normochromatic erythrocyte (MNNCE) was higher in both types of pan masala treated groups with respect to control group. Both pan masala plain and gutkha treatment significantly induced the frequency of MNPCE and MNNCE in the bone marrow cells, indicating the genotoxic potential. Furthermore, slight decline in the ratio of polychromatic erythrocytes to normochromatic erythrocytes was also noticed, suggesting the cytotoxic potential even though the ratio was statistically non significant. A dose-dependent, significant increase in chromosome aberration was observed in both types of pan masala treated mice with respect to control. However, no significant difference in micronucleus and chromosomal aberration induction was noticed between two types of pan masala exposed (PMP and PMT) groups. Results suggest that both types of pan masala, i.e. plain and gutkha, have genotoxic potential.

scholarly journals Toxic effects of various zinc dosages on poultry hemopoiesis

2020 ◽  
Vol 164 ◽  
pp. 06011
Author(s):  
Aleksandr Vishnyakov
Keyword(s):  
Bone Marrow ◽  
Broiler Chickens ◽  
Bone Marrow Cells ◽  
Marrow Cell ◽  
Ultrastructural Level ◽  
Cell Nuclei ◽  
Red Bone Marrow ◽  
Zinc Salts ◽  
Destructive Processes ◽  
Marrow Cells

The aim of the study was to determine the main patterns of the manifestation of the toxic effect of zinc chloride on the cells of the red bone marrow in the first three days after exposure. Our experiment involved broiler chickens of the “Smena-7” cross. The work presents new data on the effects of zinc salts in doses of 40 mg/kg and 60 mg/kg on the hemopoietic cells of the red bone marrow of birds at the ultrastructural level. Thus, the results of the study showed zinc poisoning of chickens even within 1-3 days led to damage to bone marrow cells. The cytoplasm of blood-forming and stromal cells of the bone marrow detects mostly signs of destructive processes, which are amplified as the duration and the dose of exposure increase. At the same time, the structure of cell nuclei often gets changed in the bone marrow. Emerging morphological signs indicate a decrease in the transcription of ribosomal RNA genes of bone marrow cells. In the bone marrow, the number of basophil erythrokaryocytes increases and the number of hemoglobinized forms of red blood cells decreases. Zinc mainly causes disorders of morpho-functional structures in erythroblasts and mature cells of other bone marrow cell lines.

scholarly journals Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
Sahdeo Prasad ◽  
Smita Srivastava ◽  
Madhulika Singh ◽  
Yogeshwer Shukla
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Positive Control ◽  
Vehicle Group ◽  
Control Group ◽  
Mouse Bone Marrow ◽  
Human Beings ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
Marrow Cells

Glyphosate (N-(phosphonomethyl) glycine,C3H8NO5P), a herbicide, used to control unwanted annual and perennial plants all over the world. Nevertheless, occupational and environmental exposure to pesticides can pose a threat to nontarget species including human beings. Therefore, in the present study, genotoxic effects of the herbicide glyphosate were analyzed by measuring chromosomal aberrations (CAs) and micronuclei (MN) in bone marrow cells of Swiss albino mice. A single dose of glyphosate was given intraperitoneally (i.p) to the animals at a concentration of 25 and 50 mg/kg b.wt. Animals of positive control group were injectedi.p. benzo(a)pyrene (100 mg/kg b.wt., once only), whereas, animals of control (vehicle) group were injectedi.p. dimethyl sulfoxide (0.2mL). Animals from all the groups were sacrificed at sampling times of 24, 48, and 72 hours and their bone marrow was analyzed for cytogenetic and chromosomal damage. Glyphosate treatment significantly increases CAs and MN induction at both treatments and time compared with the vehicle control (P<.05). The cytotoxic effects of glyphosate were also evident, as observed by significant decrease in mitotic index (MI). The present results indicate that glyphosate is clastogenic and cytotoxic to mouse bone marrow.

Erythropoietin Gene Expression in the Stromal Cell Increased by Silica to Induce Erythrocyte Differentiation

2013 ◽  
Vol 647 ◽  
pp. 494-498
Author(s):  
Wei Chung Liu ◽  
Chang Shu Tsai ◽  
Ya Yun Chen ◽  
Nien Tzu Keng
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Cells ◽  
Biological Effects ◽  
Interaction Mechanism ◽  
Silica Particles ◽  
Control Group ◽  
Stimulate Bone Marrow ◽  
Marrow Cells

Silica containing materials are often applied in bone tissue engineering, which may contact with bone marrow cells. However, the biological effects have not always been observed in studies of bone marrow cells exposed to silica. In this experiment, the relevant biological effects were evaluated. Bone marrow cells and stromal cells treated with silica particles (0.5-10 μm) were applied to investigate the possible interaction mechanism. HEL-92 cells were culture with the condition medium of stromal cells treated with or without silica particles. The erythrogenesis of bone marrow cells treated with silica particles was increased significantly. The expression level of glycophorin A the erythroid marker in HEL-92 cells treated by condition medium was higher than control group. The silica particles could also up-regulate the erythropoietin gene expression of stromal cells. The results indicate that bone marrow cells can be stimulated by silica particles to differentiate into erythrocytes. Our results suggest that silica particles can stimulate bone marrow cells to differentiate erythrocytes possibly via enhancing gene expression of erythropoietin.

scholarly journals Clinical and functional evaluation of intramyocardial implantation of autologous bone marrow cells treated with erythropoietinduring the CAD surgery (6-month results)

2019 ◽  
Vol 20 (4) ◽  
pp. 89-99
Author(s):  
A. V. Fomichev ◽  
A. M. Chernyavskiy ◽  
K. K. Gulyaeva ◽  
O. V. Poveschenko ◽  
A. P. Lykov ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Perfusion Defect ◽  
Bone Marrow Cells ◽  
Coronary Bypass ◽  
Autologous Bone Marrow ◽  
Control Group ◽  
Bypass Grafting ◽  
Coronary Bypass Grafting ◽  
Autologous Bone ◽  
Marrow Cells

Aim.Clinical and functional evaluation of the implantation of autologous bone marrow cells treated with erythropoietin in laser channels during coronary bypass grafting in patients with end-stage coronary lesion.Materials and methods.60 patients with coronary artery disease with diffuse and (or) distal right coronary artery disease were randomized into two groups: patients of group 1 (n = 30) underwent coronary bypass grafting, implantation of autologous bone marrow cells treated with erythropoietin in laser channels, patients of the 2nd group (n = 30) were operated with coronary bypass grafting of the left coronary artery system. Assessment of the clinical status, myocardial perfusion and contractility was performed initially, 6 months after the operation.Results.Six months after the operation, there was a more pronounced decrease in angina pectoris (CCS) in the main group compared to the control group, also we revealed a 6-minute walk test scores improvement. Based on two-stage scintigraphy (Tc99) in the main group before the surgical treatment, a rest perfusion defect was 8.5% [3.5, 18.5], a stress-induced perfusion defect – 7.0% [6.0, 12, 3]. In the control group, the rest defect was 9.1% [5.6, 12.4], the stress-induced perfusion defect was 7.3% [6.1, 8.7]. 6 months after surgery rest perfusion defect at the indirect revascularization group was 6.0% [2.5, 16.5] (p = 0.008), a stress-induced defect was 4.0% [1.5, 6.3] (p = 0.05). In the control group, the rest defect was 8.7% [5.3, 10.3], the stress-induced perfusion defect was 6.8% [5.3, 9.1] (p = 0.21). The results of scintigraphy with MIBG showed a left ventricle innervation defect (PID) significant decrease in the main group: initially 15.4% [14.2, 16.3], after 6 months 11.7% [9.3, 13, 2] (p = 0.045). In the control group, there was an unreliable decrease in PID: initially 14.3% [10.2, 17.3], after 6 months 13.8% [9.1, 14.2] (p = 0.14).Conclusion.Our preliminary results revealed more pronounced effect of the new indirect revascularization method expressed as in myocardial perfusion improve, myocardial sympathetic innervation restoration and clinical status improvement in comparison with control group.

scholarly journals Alterations of Hematologic and Hematopoietic Parameters in Mice Exposed to Pulsed Electromagnetic Field

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Kangchu Li ◽  
Zenghui Teng ◽  
Zhaohui Liu ◽  
Yuxing Zhang ◽  
Kaiping Long ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Electromagnetic Field ◽  
Blood Cells ◽  
Bone Marrow Cells ◽  
Exposed Group ◽  
Control Group ◽  
Pulsed Electromagnetic Field ◽  
Gm Csf ◽  
Pulsed Electromagnetic ◽  
Marrow Cells

Effects of pulsed electromagnetic field (PEMF) on hematology and hematopoiesis might vary with different PEMF parameters. The purpose of this study was to evaluate the possible effects of PEMF exposure at different pulses on hematologic and hematopoietic parameters in mice. Groups of male BALB/c mice were whole body exposed or were sham exposed (control) to PEMF at 100, 1000, and 10000 pulses. After PEMF exposure, blood samples and bone marrow cells of mice were collected for hematologic examinations, bone marrow nucleated cell counting, colony-forming units of granulocyte-macrophage (CFU-GM) colony assay, and serum granulocyte-macrophage colony-stimulating factor (GM-CSF) assay. Compared with the control group, white blood cells (WBC) and lymphocytes (LYM) in the 100 and 1000 pulses exposed groups were significantly increased but not changed in the 10000 pulses exposed group. Red blood cells (RBC), hemoglobin (HGB), and platelets (PLT) were not changed in all exposed groups. There was no significant difference in mouse bone marrow nucleated cell number between the control group and each exposed group 7 days after PEMF exposure. The CFU-GM clone number of bone marrow cells and serum GM-CSF level were significantly increased in the 100 and 1000 pulses exposed group but not changed in the 10000 pulses exposed group. Our results indicated that the PEMF exposure at fewer pulses may induce statistically significant alterations in some hematologic and hematopoietic parameters of mice but no changes can be found in the more pulses PEMF-exposed groups.

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