Faculty Opinions recommendation of Low temperature-induced systems failure in Escherichia coli: insights from rescue by cold-adapted chaperones.

2006 ◽  
Author(s):  
Antonio Juarez Gimenez
Keyword(s):  
Escherichia Coli ◽  
Low Temperature ◽  
Cold Adapted ◽  
Systems Failure

Low temperature-induced systems failure inEscherichia coli: Insights from rescue by cold-adapted chaperones

PROTEOMICS ◽  
2006 ◽  
Vol 6 (1) ◽  
pp. 193-206 ◽  
Author(s):  
Massimo Strocchi ◽  
Manuel Ferrer ◽  
Kenneth N. Timmis ◽  
Peter N. Golyshin
Keyword(s):  
Low Temperature ◽  
Inescherichia Coli ◽  
Cold Adapted ◽  
Systems Failure

Inactivation of Escherichia coli by microwave induced low temperature argon plasma treatments

2002 ◽  
Vol 53 (4) ◽  
pp. 341-346 ◽  
Author(s):  
Dambadarjaa Purevdorj ◽  
Noriyuki Igura ◽  
Isao Hayakawa ◽  
Osamu Ariyada
Keyword(s):  
Escherichia Coli ◽  
Low Temperature ◽  
Argon Plasma ◽  
Plasma Treatments

Changes in Aerobic Plate and Escherichia coli–Coliform Counts and in Populations of Inoculated Foodborne Pathogens on Inshell Walnuts during Storage

2016 ◽  
Vol 79 (7) ◽  
pp. 1143-1153 ◽  
Author(s):  
JOHN C. FRELKA ◽  
GORDON R. DAVIDSON ◽  
LINDA J. HARRIS
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Relative Humidity ◽  
Low Temperature ◽  
Foodborne Pathogens ◽  
Limit Of Detection ◽  
Sampling Time ◽  
E Coli ◽  
Plate Counts ◽  
Forced Air

ABSTRACT After harvest, inshell walnuts are dried using low-temperature forced air and are then stored in bins or silos for up to 1 year. To better understand the survival of bacteria on inshell walnuts, aerobic plate counts (APCs) and Escherichia coli–coliform counts (ECCs) were evaluated during commercial storage (10 to 12°C and 63 to 65% relative humidity) over 9 months. APCs decreased by 1.4 to 2.0 log CFU per nut during the first 5 months of storage, and ECCs decreased by 1.3 to 2.2 log CFU per nut in the first month of storage. Through the remaining 4 to 8 months of storage, APCs and ECCs remained unchanged (P > 0.05) or decreased by <0.15 log CFU per nut per month. Similar trends were observed on kernels extracted from the inshell walnuts. APCs and ECCs were consistently and often significantly higher on kernels extracted from visibly broken inshell walnuts than on kernels extracted from visibly intact inshell walnuts. Parameters measured in this study were used to determine the survival of five-strain cocktails of E. coli O157:H7, Listeria monocytogenes, and Salmonella inoculated onto freshly hulled inshell walnuts (~8 log CFU/g) after simulated commercial drying (10 to 12 h; 40°C) and simulated commercial storage (12 months at 10°C and 65% relative humidity). Populations declined by 2.86, 5.01, and 4.40 log CFU per nut for E. coli O157:H7, L. monocytogenes, and Salmonella, respectively, after drying and during the first 8 days of storage. Salmonella populations changed at a rate of −0.33 log CFU per nut per month between days 8 and 360, to final levels of 2.83 ± 0.79 log CFU per nut. E. coli and L. monocytogenes populations changed by −0.17 log CFU per nut per month and −0.26 log CFU per nut per month between days 8 and 360, respectively. For some samples, E. coli or L. monocytogenes populations were below the limit of detection by plating (0.60 log CFU per nut) by day 183 or 148, respectively; at least one of the six samples was positive at each subsequent sampling time by either plating or by enrichment.

scholarly journals Induction of Viable but Nonculturable Escherichia coli O157:H7 in the Phyllosphere of Lettuce: a Food Safety Risk Factor

2011 ◽  
Vol 77 (23) ◽  
pp. 8295-8302 ◽  
Author(s):  
Laura-Dorina Dinu ◽  
Susan Bach
Keyword(s):  
Escherichia Coli ◽  
Food Safety ◽  
Low Temperature ◽  
Cell Density ◽  
Prolonged Storage ◽  
Potential Hazard ◽  
Vbnc State ◽  
Viable But Nonculturable ◽  
Content Type ◽  
E Coli

ABSTRACTEscherichia coliO157:H7 continues to be an important human pathogen and has been increasingly linked to food-borne illness associated with fresh produce, particularly leafy greens. The aim of this work was to investigate the fate ofE. coliO157:H7 on the phyllosphere of lettuce under low temperature and to evaluate the potential hazard of viable but nonculturable (VBNC) cells induced under such stressful conditions. First, we studied the survival of six bacterial strains following prolonged storage in water at low temperature (4°C) and selected two strains with different nonculturable responses for the construction ofE. coliO157:H7 Tn7gfptransformants in order to quantitatively assess the occurrence of human pathogens on the plant surface. Under a suboptimal growth temperature (16°C), bothE. coliO157:H7 strains maintained culturability on lettuce leaves, but under more stressful conditions (8°C), the bacterial populations evolved toward the VBNC state. The strain-dependent nonculturable response was more evident in the experiments with different inoculum doses (109and 106E. coliO157:H7 bacteria per g of leaf) when strain BRMSID 188 lost culturability after 15 days and strain ATCC 43895 lost culturability within 7 days, regardless of the inoculum dose. However, the number of cells entering the VBNC state in high-cell-density inoculum (approximately 55%) was lower than in low-cell-density inoculum (approximately 70%). We recorded the presence of verotoxin for 3 days in samples that contained a VBNC population of 4 to 5 log10cells but did not detect culturable cells. These findings indicate thatE. coliO157:H7 VBNC cells are induced on lettuce plants, and this may have implications regarding food safety.

A novel cold-adapted β-galactosidase from Alteromonas sp. ML117 cleaves milk lactose effectively at low temperature

2019 ◽  
Vol 82 ◽  
pp. 94-101 ◽  
Author(s):  
Congyu Yao ◽  
Jingjing Sun ◽  
Wei Wang ◽  
Zhiwei Zhuang ◽  
Junzhong Liu ◽  
...  
Keyword(s):  
Low Temperature ◽  
Cold Adapted

scholarly journals Aliivibrio wodanis as a production host: development of genetic tools for expression of cold-active enzymes

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Jenny Johansson Söderberg ◽  
Miriam Grgic ◽  
Erik Hjerde ◽  
Peik Haugen
Keyword(s):  
Low Temperature ◽  
Protein Production ◽  
Fluorescent Protein ◽  
Active Form ◽  
Rhodococcus Erythropolis ◽  
Expression Systems ◽  
Cold Adapted ◽  
Genetic Tools ◽  
Cold Active Enzymes ◽  
Cold Active

Abstract Background Heterologous production of cold-adapted proteins currently represents one of the greatest bottlenecks in the ongoing bioprospecting efforts to find new enzymes from low-temperature environments, such as, the polar oceans that represent essentially untapped resources in this respect. In mesophilic expression hosts such as Escherichia coli, cold-adapted enzymes often form inactive aggregates. Therefore it is necessary to develop new low-temperature expression systems, including identification of new host organisms and complementary genetic tools. Psychrophilic bacteria, including Pseudoalteromonas haloplanktis, Shewanella and Rhodococcus erythropolis have all been explored as candidates for such applications. However to date none of these have found widespread use as efficient expression systems, or are commercially available. In the present work we explored the use of the sub-Arctic bacterium Aliivibrio wodanis as a potential host for heterologous expression of cold-active enzymes. Results We tested 12 bacterial strains, as well as available vectors, promoters and reporter systems. We used RNA-sequencing to determine the most highly expressed genes and their intrinsic promoters in A. wodanis. In addition we examined a novel 5′-fusion to stimulate protein production and solubility. Finally we tested production of a set of “difficult-to-produce” enzymes originating from various bacteria and one Archaea. Our results show that cold-adapted enzymes can be produced in soluble and active form, even in cases when protein production failed in E. coli due to the formation of inclusion bodies. Moreover, we identified a 60-bp/20-aa fragment from the 5′-end of the AW0309160_00174 gene that stimulates expression of Green Fluorescent Protein and improves production of cold-active enzymes when used as a 5′-fusion. A 25-aa peptide from the same protein enhanced secretion of a 25-aa-sfGFP fusion. Conclusions Our results indicate the use of A. wodanis and associated genetic tools for low-temperature protein production and indicate that A. wodanis represents an interesting platform for further development of a protein production system that can promote further cold-enzyme discoveries.

High rate domestic wastewater treatment at 15 °C using anaerobic reactors inoculated with cold-adapted sediments/soils – shaping robust methanogenic communities

2019 ◽  
Vol 5 (1) ◽  
pp. 70-82 ◽  
Author(s):  
Evangelos Petropoulos ◽  
Yongjie Yu ◽  
Shamas Tabraiz ◽  
Aminu Yakubu ◽  
Thomas P. Curtis ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Low Temperature ◽  
Domestic Wastewater ◽  
High Rate ◽  
Cold Adapted ◽  
Anaerobic Reactors ◽  
Domestic Wastewater Treatment

To choose the reactor format in which to employ a low temperature adapted seed for wastewater treatment, we compared a UASB and an AnMBRUASB (UF)reactor at low HRT and temperature (15 °C).

Structure analysis of a glycosides hydrolase family 42 cold-adapted β-galactosidase from Rahnella sp. R3

RSC Advances ◽  
2016 ◽  
Vol 6 (44) ◽  
pp. 37362-37369 ◽  
Author(s):  
Yuting Fan ◽  
Jiang Yi ◽  
Xiao Hua ◽  
Yinghui Feng ◽  
Ruijin Yang ◽  
...  
Keyword(s):  
Low Temperature ◽  
High Activity ◽  
Structure Analysis ◽  
Dairy Industry ◽  
Cold Adapted ◽  
Psychrotrophic Bacterium ◽  
Hydrolase Family

The β-galactosidase isolated from a psychrotrophic bacterium, Rahnella sp. R3 (R-β-Gal), exhibits high activity at low temperature and has potential in the dairy industry.

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