Faculty Opinions recommendation of Transcriptome analysis of all two-component regulatory system mutants of Escherichia coli K-12.

2002 ◽  
Author(s):  
Stephen Busby
Keyword(s):  
Escherichia Coli ◽  
Transcriptome Analysis ◽  
Regulatory System ◽  
Two Component ◽  
K 12

Transcriptome analysis of all two-component regulatory system mutants of Escherichia coli K-12

2002 ◽  
Vol 46 (1) ◽  
pp. 281-291 ◽  
Author(s):  
Taku Oshima ◽  
Hirofumi Aiba ◽  
Yasushi Masuda ◽  
Shigehiko Kanaya ◽  
Masahito Sugiura ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Transcriptome Analysis ◽  
Regulatory System ◽  
Two Component ◽  
K 12

scholarly journals Identification of a Copper-Responsive Two-Component System on the Chromosome of Escherichia coli K-12

2000 ◽  
Vol 182 (20) ◽  
pp. 5864-5871 ◽  
Author(s):  
George P. Munson ◽  
Deborah L. Lam ◽  
F. Wayne Outten ◽  
Thomas V. O'Halloran
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Syringae ◽  
Metal Ion ◽  
Copper Ions ◽  
Copper Ion ◽  
Regulatory System ◽  
Copper Resistance ◽  
E Coli ◽  
Two Component ◽  
K 12

ABSTRACT Using a genetic screen we have identified two chromosomal genes,cusRS (ylcA ybcZ), from Escherichia coli K-12 that encode a two-component, signal transduction system that is responsive to copper ions. This regulatory system is required for copper-induced expression of pcoE, a plasmid-borne gene from the E. coli copper resistance operon pco. The closest homologs of CusR and CusS are plasmid-borne two-component systems that are also involved in metal responsive gene regulation: PcoR and PcoS from the pcooperon of E. coli; CopR and CopS from thecop operon, which provides copper resistance toPseudomonas syringae; and SilR and SilS from thesil locus, which provides silver ion resistance toSalmonella enterica serovar Typhimurium. The genescusRS are also required for the copper-dependent expression of at least one chromosomal gene, designated cusC(ylcB), which is allelic to the recently identified virulence gene ibeB in E. coli K1. Thecus locus may comprise a copper ion efflux system, because the expression of cusC is induced by high concentrations of copper ions. Furthermore, the translation products of cusCand additional downstream genes are homologous to known metal ion antiporters.

scholarly journals YieJ (CbrC) Mediates CreBC-Dependent Colicin E2 Tolerance in Escherichia coli

2010 ◽  
Vol 192 (13) ◽  
pp. 3329-3336 ◽  
Author(s):  
S. James L. Cariss ◽  
Chrystala Constantinidou ◽  
Mala D. Patel ◽  
Yuiko Takebayashi ◽  
Jon L. Hobman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Microarray Analysis ◽  
Gene Knockout ◽  
Inner Membrane ◽  
Two Component ◽  
Inner Membrane Protein ◽  
Colicin E2 ◽  
K 12

ABSTRACT Colicin E2-tolerant (known as Cet2) Escherichia coli K-12 mutants overproduce an inner membrane protein, CreD, which is believed to cause the Cet2 phenotype. Here, we show that overproduction of CreD in a Cet2 strain results from hyperactivation of the CreBC two-component regulator, but CreD overproduction is not responsible for the Cet2 phenotype. Through microarray analysis and gene knockout and overexpression studies, we show that overexpression of another CreBC-regulated gene, yieJ (also known as cbrC), causes the Cet2 phenotype.

A Chimeric Two-Component Regulatory System-Based Escherichia coli Biosensor Engineered to Detect Glutamate

2018 ◽  
Vol 186 (2) ◽  
pp. 335-349 ◽  
Author(s):  
Sambandam Ravikumar ◽  
Yokimiko David ◽  
Si Jae Park ◽  
Jong-il Choi
Keyword(s):  
Escherichia Coli ◽  
Regulatory System ◽  
Two Component

scholarly journals Transcriptome Analysis of the Brucella abortus BvrR/BvrS Two-Component Regulatory System

PLoS ONE ◽  
2010 ◽  
Vol 5 (4) ◽  
pp. e10216 ◽  
Author(s):  
Cristina Viadas ◽  
María C. Rodríguez ◽  
Felix J. Sangari ◽  
Jean-Pierre Gorvel ◽  
Juan M. García-Lobo ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Brucella Abortus ◽  
Regulatory System ◽  
Two Component

scholarly journals Employment of a Promoter-Swapping Technique Shows that PhoU Modulates the Activity of the PstSCAB2 ABC Transporter in Escherichia coli

2008 ◽  
Vol 75 (3) ◽  
pp. 573-582 ◽  
Author(s):  
Christopher D. Rice ◽  
Jacob E. Pollard ◽  
Zachery T. Lewis ◽  
William R. McCleary
Keyword(s):  
Escherichia Coli ◽  
Regulatory System ◽  
Negative Regulator ◽  
Growth Defect ◽  
Pho Regulon ◽  
E Coli ◽  
Regulatory Module ◽  
Two Component ◽  
Severe Growth Defect ◽  
Promoter Swapping

ABSTRACT Expression of the Pho regulon in Escherichia coli is induced in response to low levels of environmental phosphate (Pi). Under these conditions, the high-affinity PstSCAB2 protein (i.e., with two PstB proteins) is the primary Pi transporter. Expression from the pstSCAB-phoU operon is regulated by the PhoB/PhoR two-component regulatory system. PhoU is a negative regulator of the Pho regulon; however, the mechanism by which PhoU accomplishes this is currently unknown. Genetic studies of phoU have proven to be difficult because deletion of the phoU gene leads to a severe growth defect and creates strong selection for compensatory mutations resulting in confounding data. To overcome the instability of phoU deletions, we employed a promoter-swapping technique that places expression of the phoBR two-component system under control of the Ptac promoter and the lacO ID regulatory module. This technique may be generally applicable for controlling expression of other chromosomal genes in E. coli. Here we utilized PphoB ::Ptac and PpstS ::Ptac strains to characterize phenotypes resulting from various ΔphoU mutations. Our results indicate that PhoU controls the activity of the PstSCAB2 transporter, as well as its abundance within the cell. In addition, we used the PphoB ::Ptac ΔphoU strain as a platform to begin characterizing new phoU mutations in plasmids.

scholarly journals Fumarate Regulation of Gene Expression in Escherichia coli by the DcuSR (dcuSR Genes) Two-Component Regulatory System

1998 ◽  
Vol 180 (20) ◽  
pp. 5421-5425 ◽  
Author(s):  
Evelyn Zientz ◽  
Johannes Bongaerts ◽  
Gottfried Unden
Keyword(s):  
Escherichia Coli ◽  
Histidine Kinase ◽  
Response Regulator ◽  
Regulation Of Gene Expression ◽  
Regulatory System ◽  
E Coli ◽  
Expression In Escherichia Coli ◽  
Two Component ◽  
Genes Encoding ◽  
Periplasmic Domain

ABSTRACT In Escherichia coli the genes encoding the anaerobic fumarate respiratory system are transcriptionally regulated by C4-dicarboxylates. The regulation is effected by a two-component regulatory system, DcuSR, consisting of a sensory histidine kinase (DcuS) and a response regulator (DcuR). DcuS and DcuR are encoded by the dcuSR genes (previouslyyjdHG) at 93.7 min on the calculated E. coli map. Inactivation of the dcuR anddcuS genes caused the loss of C4-dicarboxylate-stimulated synthesis of fumarate reductase (frdABCD genes) and of the anaerobic fumarate-succinate antiporter DcuB (dcuB gene). DcuS is predicted to contain a large periplasmic domain as the supposed site for C4-dicarboxylate sensing. Regulation by DcuR and DcuS responded to the presence of the C4-dicarboxylates fumarate, succinate, malate, aspartate, tartrate, and maleate. Since maleate is not taken up by the bacteria under these conditions, the carboxylates presumably act from without. Genes of the aerobic C4-dicarboxylate pathway encoding succinate dehydrogenase (sdhCDAB) and the aerobic succinate carrier (dctA) are only marginally or negatively regulated by the DcuSR system. The CitAB two-component regulatory system, which is highly similar to DcuSR, had no effect on C4-dicarboxylate regulation of any of the genes.

scholarly journals The Cpx Envelope Stress Response Is Controlled by Amplification and Feedback Inhibition

1999 ◽  
Vol 181 (17) ◽  
pp. 5263-5272 ◽  
Author(s):  
Tracy L. Raivio ◽  
Daniel L. Popkin ◽  
Thomas J. Silhavy
Keyword(s):  
Escherichia Coli ◽  
Stress Response ◽  
Virulence Factors ◽  
Histidine Kinase ◽  
Feedback Inhibition ◽  
Response Regulator ◽  
Regulatory System ◽  
Concomitant Increase ◽  
Envelope Stress ◽  
Two Component

ABSTRACT In Escherichia coli, the Cpx two-component regulatory system activates expression of protein folding and degrading factors in response to misfolded proteins in the bacterial envelope (inner membrane, periplasm, and outer membrane). It is comprised of the histidine kinase CpxA and the response regulator CpxR. This response plays a role in protection from stresses, such as elevated pH, as well as in the biogenesis of virulence factors. Here, we show that the Cpx periplasmic stress response is subject to amplification and repression through positive and negative autofeedback mechanisms. Western blot and operon fusion analyses demonstrated that the cpxRA operon is autoactivated. Conditions that lead to elevated levels of phosphorylated CpxR cause a concomitant increase in transcription ofcpxRA. Conversely, overproduction of CpxP, a small, Cpx-regulated protein of previously unknown function, represses the regulon and can block activation of the pathway. This repression is dependent on an intact CpxA sensing domain. The ability to autoactivate and then subsequently repress allows for a temporary amplification of the Cpx response that may be important in rescuing cells from transitory stresses and cueing the appropriately timed elaboration of virulence factors.

Sign in / Sign up

Export Citation Format

Share Document