Faculty Opinions recommendation of Trafficking of STEVOR to the Maurer's clefts in Plasmodium falciparum-infected erythrocytes.

2005 ◽  
Author(s):  
Karin Romisch
Keyword(s):  
Plasmodium Falciparum ◽  
Maurer's Clefts

scholarly journals Trafficking and Association of Plasmodium falciparum MC-2TM with the Maurer’s Clefts

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 431
Author(s):  
Raghavendra Yadavalli ◽  
John W. Peterson ◽  
Judith A. Drazba ◽  
Tobili Y. Sam-Yellowe
Keyword(s):  
Plasmodium Falciparum ◽  
Erythrocyte Membrane ◽  
Sodium Carbonate ◽  
Infected Erythrocyte ◽  
Brefeldin A ◽  
Specific Expression ◽  
Lipid Interactions ◽  
And Topology ◽  
Streptolysin O ◽  
Maurer's Clefts

In this study, we investigated stage specific expression, trafficking, solubility and topology of endogenous PfMC-2TM in P. falciparum (3D7) infected erythrocytes. Following Brefeldin A (BFA) treatment of parasites, PfMC-2TM traffic was evaluated using immunofluorescence with antibodies reactive with PfMC-2TM. PfMC-2TM is sensitive to BFA treatment and permeabilization of infected erythrocytes with streptolysin O (SLO) and saponin, showed that the N and C-termini of PfMC-2TM are exposed to the erythrocyte cytoplasm with the central portion of the protein protected in the MC membranes. PfMC-2TM was expressed as early as 4 h post invasion (hpi), was tightly colocalized with REX-1 and trafficked to the erythrocyte membrane without a change in solubility. PfMC-2TM associated with the MC and infected erythrocyte membrane and was resistant to extraction with alkaline sodium carbonate, suggestive of protein-lipid interactions with membranes of the MC and erythrocyte. PfMC-2TM is an additional marker of the nascent MCs.

scholarly journals The Cytoplasmic Region of Plasmodium falciparum SURFIN4.2 Is Required for Transport from Maurer’s Clefts to the Red Blood Cell Surface

2015 ◽  
Vol 43 (4) ◽  
pp. 265-272 ◽  
Author(s):  
Wataru Kagaya ◽  
Shinya Miyazaki ◽  
Kazuhide Yahata ◽  
Nobuo Ohta ◽  
Osamu Kaneko
Keyword(s):  
Plasmodium Falciparum ◽  
Cell Surface ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Cytoplasmic Region ◽  
Maurer's Clefts

A novel Plasmodium falciparum ring stage protein, REX, is located in Maurer’s clefts

2004 ◽  
Vol 136 (2) ◽  
pp. 181-189 ◽  
Author(s):  
Paula L. Hawthorne ◽  
Katharine R. Trenholme ◽  
Tina S. Skinner-Adams ◽  
Tobias Spielmann ◽  
Katja Fischer ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Ring Stage ◽  
Maurer's Clefts

scholarly journals Plasmodium falciparum STEVOR Proteins Are Highly Expressed in Patient Isolates and Located in the Surface Membranes of Infected Red Blood Cells and the Apical Tips of Merozoites

2008 ◽  
Vol 76 (7) ◽  
pp. 3329-3336 ◽  
Author(s):  
Jane E. Blythe ◽  
Xue Yan Yam ◽  
Claudia Kuss ◽  
Zbynek Bozdech ◽  
Anthony A. Holder ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Multigene Family ◽  
Blood Cells ◽  
Antigenic Variation ◽  
Human Parasite ◽  
Host Parasite Interactions ◽  
Maurer's Clefts ◽  
Host Parasite ◽  
Family Code

ABSTRACT The human parasite Plasmodium falciparum has the potential to express a vast repertoire of variant proteins on the surface of the infected red blood cell (iRBC). Variation in the expression pattern of these proteins is linked to antigenic variation and thereby evasion of host antibody-mediated immunity. The genes in the stevor multigene family code for small variant antigens that are expressed in blood-stage parasites where they can be detected in membranous structures called Maurer's clefts (MC). Some studies have indicated that STEVOR protein may also be trafficked to the iRBC membrane. To address the location of STEVOR protein in more detail, we have analyzed expression in several cultured parasite lines and in parasites obtained directly from patients. We detected STEVOR expression in a higher proportion of parasites recently isolated from patients than in cultured parasite lines and show that STEVOR is trafficked in schizont-stage parasites from the MC to the RBC cytosol and the iRBC membrane. Furthermore, STEVOR protein is also detected at the apical end of merozoites. Importantly, we show that culture-adapted parasites do not require STEVOR for survival. These findings provide new insights into the role of the stevor multigene family during both the schizont and merozoite stages of the parasite and highlight the importance of studying freshly isolated parasites, rather than parasite lines maintained in culture, when investigating potential mediators of host-parasite interactions.

scholarly journals Plasmodium falciparum Antigen 332 Is a Resident Peripheral Membrane Protein of Maurer's Clefts

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e46980 ◽  
Author(s):  
Sandra Nilsson ◽  
Davide Angeletti ◽  
Mats Wahlgren ◽  
Qijun Chen ◽  
Kirsten Moll
Keyword(s):  
Plasmodium Falciparum ◽  
Membrane Protein ◽  
Peripheral Membrane Protein ◽  
Maurer's Clefts

LANCL1, an erythrocyte protein recruited to the Maurer's clefts during Plasmodium falciparum development

2005 ◽  
Vol 141 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Thierry Blisnick ◽  
Laetitia Vincensini ◽  
Jean Christophe Barale ◽  
Abdelkader Namane ◽  
Catherine Braun Breton
Keyword(s):  
Plasmodium Falciparum ◽  
Maurer's Clefts

Maurer's clefts: A novel multi-functional organelle in the cytoplasm of Plasmodium falciparum-infected erythrocytes

2006 ◽  
Vol 36 (1) ◽  
pp. 23-36 ◽  
Author(s):  
Michael Lanzer ◽  
Hannes Wickert ◽  
Georg Krohne ◽  
Laetitia Vincensini ◽  
Catherine Braun Breton
Keyword(s):  
Plasmodium Falciparum ◽  
Maurer's Clefts

A network-based approach reveals novel invasion and Maurer's clefts-related proteins in Plasmodium falciparum

2019 ◽  
Vol 15 (6) ◽  
pp. 431-441 ◽  
Author(s):  
Dibyajyoti Das ◽  
Sowmya Ramaswamy Krishnan ◽  
Arijit Roy ◽  
Gopalakrishnan Bulusu
Keyword(s):  
Plasmodium Falciparum ◽  
Protein Interaction ◽  
Protein Interaction Network ◽  
Interaction Network ◽  
Disease Pathogenesis ◽  
Protein Protein Interaction ◽  
Novel Proteins ◽  
Maurer's Clefts ◽  
Related Proteins ◽  
Protein Protein Interaction Network

To understand disease pathogenesis, all the disease-related proteins must be identified. In this work, known proteins were used to identify related novel proteins using RWR method on a dynamic P. falciparum protein–protein interaction network.

Characterisation of Plasmodium falciparum RESA-like protein peptides that bind specifically to erythrocytes and inhibit invasion

2007 ◽  
Vol 388 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Luis Eduardo Rodriguez ◽  
Ricardo Vera ◽  
John Valbuena ◽  
Hernando Curtidor ◽  
Javier Garcia ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Membrane Surface ◽  
Binding Assays ◽  
Gene Encoding ◽  
Trophozoite Stage ◽  
Rbc Membrane ◽  
Erythrocyte Invasion ◽  
Erythrocyte Surface ◽  
Maurer's Clefts

Abstract The Plasmodium falciparum ring-erythrocyte surface antigen (RESA)-like putative protein was identified and characterised. PCR and RT-PCR assays revealed that the gene encoding this protein was both present and being transcribed in P. falciparum strain FCB-2 16 h after erythrocyte invasion. Indirect immunofluorescence studies detected this protein in infected erythrocyte (IE) cytosol in dense fluorescent granules similar to Maurer's clefts at 16–20 h (parasites in ring and trophozoite stages) and very strongly on IE membranes at 22 h, suggesting that it is synthesised during early ring stages (16 h) and transported to the infected red blood cell (RBC) membrane surface during the trophozoite stage (22 h). Western blotting showed that antisera produced against polymerised synthetic peptides of this protein recognised a 72-kDa band in P. falciparum schizont lysate. P. falciparum RESA-like peptides used in normal RBC binding assays revealed that peptides 30326 (101NAEKI LGFDD KNILE ALDLFY120), 30334 (281RVTWK KLRTK MIKAL KKSLTY300) and 30342 (431SSPQR LKFTA GGGFC GKLRNY450) bind with high activity and saturability, presenting nM affinity constants. These peptides contain α-helical structural elements, as determined by circular dichroism, and inhibit P. falciparum in vitro invasion of normal RBCs by up to 91%, suggesting that some RESA-like protein regions are involved in intra-erythrocyte stage P. falciparum invasion.

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