Faculty Opinions recommendation of Functional genomic studies of uropathogenic Escherichia coli and host urothelial cells when intracellular bacterial communities are assembled.

2007 ◽  
Author(s):  
Antonio Juarez Gimenez
Keyword(s):  
Escherichia Coli ◽  
Bacterial Communities ◽  
Uropathogenic Escherichia Coli ◽  
Functional Genomic ◽  
Urothelial Cells ◽  
Genomic Studies ◽  
Intracellular Bacterial Communities

scholarly journals Involvement of biofilm-like in tracellular bacterial communities of uropathogenic Escherichia coli in pathogenesis of urinary tract infections – a mini review

2013 ◽  
Vol 26 (3) ◽  
pp. 321-325
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Bacterial Communities ◽  
Uropathogenic Escherichia Coli ◽  
E Coli ◽  
Etiologic Agents ◽  
Tract Infections ◽  
Intracellular Bacterial Communities

This paper presents a precisely defined question about the role of the biofilm-like intracellular bacterial communities in pathogenesis of the urinary tract infections. According to the recent literature, uropathogenic Escherichia coli is one of the leading etiologic agents of the urinary tract infections. Although E. coli is regarded as an extracellular pathogen, some experiments have revealed a multi-step infection cycle, which involves adhesion, invasion, proliferation within invaded urothelial cell in the form of biofilm-like intracellular bacterial communities and dispersal, leading to infection of next neighbouring cells. Therefore, the prevention and treatment of the urinary tract infections must include intracellular stage of infection.

Functional genomic studies of dihydroxyacetone utilization in Escherichia coli

Microbiology ◽  
2000 ◽  
Vol 146 (10) ◽  
pp. 2343-2344 ◽  
Author(s):  
I. T. Paulsen ◽  
J. Reizer ◽  
R. Z. Jin ◽  
E. C. C. Lin ◽  
M. H. Saier, Jr
Keyword(s):  
Escherichia Coli ◽  
Functional Genomic ◽  
Genomic Studies

scholarly journals Purification of Intracellular Bacterial Communities during Experimental Urinary Tract Infection Reveals an Abundant and Viable Bacterial Reservoir

2018 ◽  
Vol 86 (4) ◽  
Author(s):  
Suhanya Duraiswamy ◽  
Jacqueline L. Y. Chee ◽  
Siyi Chen ◽  
Enjun Yang ◽  
Kristin Lees ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Bacterial Communities ◽  
Antibiotic Use ◽  
Isolation Technique ◽  
Content Type ◽  
Recurrent Uti ◽  
Molecular Studies ◽  
Genomic Studies ◽  
Tract Infections ◽  
Intracellular Bacterial Communities

ABSTRACTUrinary tract infections (UTIs) are a major infection of humans, particularly affecting women. Recurrent UTIs can cause significant discomfort and expose patients to high levels of antibiotic use, which in turn contributes to the development of higher antibiotic resistance rates. Most UTIs are caused by uropathogenicEscherichia coli, which is able to form intracellular collections (termed intracellular bacterial communities [IBCs]) within the epithelial cells lining the bladder lumen. IBCs are seen in both infected mice and humans and are a potential cause of recurrent UTI. Genetic and molecular studies of IBCs have been hampered both by the low number of bacteria in IBCs relative to the number extracellular bacteria and by population bottlenecks that occur during IBC formation. We now report the development of a simple and rapid technique for isolating pure IBCs from experimentally infected mice. We verified the specificity and purity of the isolated IBCs via microscopy, gene expression, and culture-based methods. Our results further demonstrated that our isolation technique practically enables specific molecular studies of IBCs. In the first such direct measurement, we determined that a single epithelial cell containing an early IBC typically contains 103viable bacteria. Our isolation technique complements recent progress in low-input, single-cell genomics to enable future genomic studies of the formation of IBCs and their activation pathways during recurrent UTI, which may lead to novel strategies to eliminate them from the bladder.

scholarly journals Novel Three-Dimensional Organoid Model for Evaluation of the Interaction of Uropathogenic Escherichia coli with Terminally Differentiated Human Urothelial Cells

2006 ◽  
Vol 74 (1) ◽  
pp. 750-757 ◽  
Author(s):  
Yarery C. Smith ◽  
Kerian K. Grande ◽  
Susan B. Rasmussen ◽  
Alison D. O'Brien
Keyword(s):  
Escherichia Coli ◽  
Three Dimensional ◽  
Uropathogenic Escherichia Coli ◽  
Urothelial Cells ◽  
Human Bladder ◽  
Upec Strain ◽  
Tissue Specific ◽  
Microgravity Conditions ◽  
Uroepithelial Cells

ABSTRACT Human bladder 5637 cells cultivated under microgravity conditions formed organoids that displayed characteristics of in vivo tissue-specific differentiation. Uropathogenic Escherichia coli (UPEC) strain CP9 colonized and penetrated the organoids and induced α-hemolysin-mediated exfoliation of uroepithelial cells. We propose these uro-organoids as models that simulate the interactions between UPEC and terminally differentiated human urothelium.

1368 SURFACTANT PROTEINS A AND D INHIBIT UROPATHOGENIC ESCHERICHIA COLI BINDING TO UROTHELIAL CELLS

2011 ◽  
Vol 185 (4S) ◽  
Author(s):  
Yuichiro Kurimura ◽  
Teruhisa Uehara ◽  
Kohji Ichihara ◽  
Jiro Hashimoto ◽  
Satoshi Takahashi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Uropathogenic Escherichia Coli ◽  
Surfactant Proteins ◽  
Urothelial Cells

scholarly journals Uropathogenic E. coli induces DNA damage in the bladder

2020 ◽  
Author(s):  
Camille V. Chagneau ◽  
Clémence Massip ◽  
Nadège Bossuet-Greif ◽  
Christophe Fremez ◽  
Jean-Paul Motta ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Escherichia Coli ◽  
Dna Damage ◽  
Bladder Cancer ◽  
Urinary Tract ◽  
Bacterial Communities ◽  
E Coli ◽  
Bladder Cells ◽  
Tract Infections ◽  
Intracellular Bacterial Communities

AbstractUrinary tract infections (UTIs) are among the most common outpatient infections, with a lifetime incidence of around 60% in women. We analysed urine samples from 223 patients with community-acquired UTIs and report the presence of a metabolite released during the synthesis of colibactin, a bacterial genotoxin, in 50 of the samples examined. Uropathogenic Escherichia coli strains isolated from these patients, as well as the archetypal E. coli strain UTI89, were found to produce colibactin. In a murine model of UTI, the machinery producing colibactin was expressed during the early hours of the infection, when intracellular bacterial communities form. We observed extensive DNA damage both in umbrella and bladder progenitor cells. To the best of our knowledge this is the first report of colibactin production in UTIs in humans and its genotoxicity in bladder cells. This bacterial genotoxin, which is increasingly suspected to promote colorectal cancer, should also be scrutinised in the context of bladder cancer.

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