Faculty Opinions recommendation of Cheese rind communities provide tractable systems for in situ and in vitro studies of microbial diversity.

2016 ◽  
Author(s):  
Lars Jelsbak
Keyword(s):  
Microbial Diversity ◽  
In Vitro Studies

scholarly journals Cheese Rind Communities Provide Tractable Systems for In Situ and In Vitro Studies of Microbial Diversity

Cell ◽  
2014 ◽  
Vol 158 (2) ◽  
pp. 422-433 ◽  
Author(s):  
Benjamin E. Wolfe ◽  
Julie E. Button ◽  
Marcela Santarelli ◽  
Rachel J. Dutton
Keyword(s):  
Microbial Diversity ◽  
In Vitro Studies

scholarly journals Effect of Cavity Disinfectants on Adhesion to Primary Teeth—A Systematic Review

2021 ◽  
Vol 22 (9) ◽  
pp. 4398
Author(s):  
Ana Coelho ◽  
Inês Amaro ◽  
Ana Apolónio ◽  
Anabela Paula ◽  
José Saraiva ◽  
...  
Keyword(s):  
Primary Teeth ◽  
Clinical Success ◽  
Dental Materials ◽  
In Vitro Study ◽  
In Vitro Studies ◽  
Cochrane Library ◽  
Promising Alternative

Some authors have been proposing the use of cavity disinfectants in order to reduce, or even eliminate, the effect of the microorganisms present in a dental cavity before a restoration is placed. The aim of this study was to evaluate the effect of different cavity disinfectants on bond strength and clinical success of composite and glass ionomer restorations on primary teeth. The research was conducted using Cochrane Library, PubMed/MEDLINE, SCOPUS, and Web of Science for articles published up to February 2021. The search was performed according to the PICO strategy. The evaluation of the methodological quality of each in vitro study was assessed using the CONSORT checklist for reporting in vitro studies on dental materials. Sixteen in vitro studies and one in situ study fulfilled the inclusion criteria and were analyzed. Chlorhexidine was the most studied cavity disinfectant, and its use does not compromise dentin bonding. Sodium hypochlorite is a promising alternative, but more research on its use is required to clearly state that it can safely be used as a cavity disinfectant for primary teeth. Although other disinfectants were studied, there is a low-level evidence attesting their effects on adhesion, therefore their use should be avoided.

scholarly journals MICROSPECTROPHOTOMETRY OF NEWLY SYNTHESIZED STARCH IN SITU

1970 ◽  
Vol 18 (6) ◽  
pp. 439-449
Author(s):  
GEORGE E. WHEELER
Keyword(s):  
Absorption Maximum ◽  
In Vitro Studies ◽  
Phosphate Solution ◽  
Starch Digestion ◽  
Spectral Curves ◽  
Similarities And Differences

Many of the cells in stem sections of several Commelinaceae species synthesized much new starch when incubated in buffered 1% glucose 1-phosphate solution. The starch appeared in the cytoplasm rather than in the plastids. Although the starch I2-KI color was uniform within any one cell, there was considerable variation from cell to cell, even in the same section. The colors with I2-KI ranged from blue, through purples to magenta and mahogany. Tests with α-amylase and with β-amylase showed the starch to be amylose. Microspectrophotometrically determined extinction curves, based on the new starch in situ, varied with the visualized color. As expected, starch which stained blue with I2-KI had an absorption maximum in the orange-red wavelengths above 600 mµ; increasingly red I2-KI colors were characterized by shifts of the absorption maximum further into the shorter wavelengths. The course of new starch digestion by α-amylase and by β-amylase was followed visually and with the microspectrophotometer. Similarities and differences between these spectral curves and those published for in vitro studies are pointed out. The difficulties met with in using the microspectrophotometric method are discussed.

Polarized light scattering of nucleosomes and polynucleosomes-in situ and in vitro studies

1991 ◽  
Vol 38 (7) ◽  
pp. 670-678 ◽  
Author(s):  
A. Diaspro ◽  
M. Bertolotto ◽  
L. Vergani ◽  
C. Nicolini
Keyword(s):  
Light Scattering ◽  
Polarized Light ◽  
In Vitro Studies

scholarly journals Mature Dendritic Cells Infiltrate the T Cell-Rich Region of Oral Mucosa in Chronic Periodontitis: In Situ, In Vivo, and In Vitro Studies

2001 ◽  
Vol 167 (8) ◽  
pp. 4693-4700 ◽  
Author(s):  
Ravi Jotwani ◽  
Anna Karolina Palucka ◽  
Montasr Al-Quotub ◽  
Mahyar Nouri-Shirazi ◽  
Jay Kim ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Oral Mucosa ◽  
Chronic Periodontitis ◽  
In Vitro Studies ◽  
Rich Region

scholarly journals Optimization of the In Situ Laser Fenestration of Stent Graft: In Vitro Studies

2018 ◽  
Vol 68 (3) ◽  
pp. e91
Author(s):  
Jing Lin ◽  
Niraj Parikh ◽  
Lu Wang ◽  
Mark Nutley ◽  
Jean Panneton ◽  
...  
Keyword(s):  
Stent Graft ◽  
In Vitro Studies

scholarly journals The diversity and function of sourdough starter microbiomes

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Elizabeth A Landis ◽  
Angela M Oliverio ◽  
Erin A McKenney ◽  
Lauren M Nichols ◽  
Nicole Kfoury ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
Acetic Acid Bacteria ◽  
Fermented Food ◽  
Microbial Interactions ◽  
Structure Variation ◽  
Biogeographic Patterns ◽  
And Function ◽  
Occurrence Patterns

Humans have relied on sourdough starter microbial communities to make leavened bread for thousands of years, but only a small fraction of global sourdough biodiversity has been characterized. Working with a community-scientist network of bread bakers, we determined the microbial diversity of 500 sourdough starters from four continents. In sharp contrast with widespread assumptions, we found little evidence for biogeographic patterns in starter communities. Strong co-occurrence patterns observed in situ and recreated in vitro demonstrate that microbial interactions shape sourdough community structure. Variation in dough rise rates and aromas were largely explained by acetic acid bacteria, a mostly overlooked group of sourdough microbes. Our study reveals the extent of microbial diversity in an ancient fermented food across diverse cultural and geographic backgrounds.

Treatments with Nisin and Chelators to Reduce Salmonella and Escherichia coli on Beef†

1995 ◽  
Vol 58 (9) ◽  
pp. 1028-1030 ◽  
Author(s):  
CATHERINE N. CUTTER ◽  
GREGORY R. SIRAGUSA
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Chelating Agents ◽  
In Vitro Studies ◽  
Sodium Hexametaphosphate ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Lean Beef

Salmonella typhimurium ATCC 14028 or Escherichia coli O157:H7 attached to lean beef tissue were treated with citrate, lactate, sodium hexametaphosphate, or EDTA, alone or in combination with nisin in simple buffers, and incubated at 4°C for up to 3 days. Lactate with nisin reduced S. typhimurium attached to beef by 040 log10 CFU/cm2, while EDTA and nisin reduced E. coli O157:H7 by 0.42 log10 CFU/cm2. Unlike earlier in vitro studies in which treatments with nisin and chelating agents resulted in reductions of > 4 log10 CFU/cm2, such reductions were not observed in situ.

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