Effect of Cavity Disinfectants on Adhesion to Primary Teeth—A Systematic Review

Some authors have been proposing the use of cavity disinfectants in order to reduce, or even eliminate, the effect of the microorganisms present in a dental cavity before a restoration is placed. The aim of this study was to evaluate the effect of different cavity disinfectants on bond strength and clinical success of composite and glass ionomer restorations on primary teeth. The research was conducted using Cochrane Library, PubMed/MEDLINE, SCOPUS, and Web of Science for articles published up to February 2021. The search was performed according to the PICO strategy. The evaluation of the methodological quality of each in vitro study was assessed using the CONSORT checklist for reporting in vitro studies on dental materials. Sixteen in vitro studies and one in situ study fulfilled the inclusion criteria and were analyzed. Chlorhexidine was the most studied cavity disinfectant, and its use does not compromise dentin bonding. Sodium hypochlorite is a promising alternative, but more research on its use is required to clearly state that it can safely be used as a cavity disinfectant for primary teeth. Although other disinfectants were studied, there is a low-level evidence attesting their effects on adhesion, therefore their use should be avoided.

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The Influence of Irrigation during the Finishing and Polishing of Composite Resin Restorations—A Systematic Review of In Vitro Studies

Materials ◽

10.3390/ma14071675 ◽

2021 ◽

Vol 14 (7) ◽

pp. 1675

Author(s):

João Paulo Silva ◽

Ana Coelho ◽

Anabela Paula ◽

Inês Amaro ◽

José Saraiva ◽

...

Keyword(s):

Systematic Review ◽

Composite Resin ◽

Dental Materials ◽

In Vitro Studies ◽

Cochrane Library ◽

Polishing Procedures ◽

Composite Resin Restorations ◽

Resin Restorations

The surface smoothness of composite restorations affects not only their esthetic appearance but also other properties. Thus, rough surfaces can lead to staining, plaque accumulation, gingival irritation, recurrent caries, abrasiveness, wear kinetics, and tactile perception by the patient. The aim of this study was to evaluate the influence of irrigation during the finishing and polishing of composite resin restorations. A systematic search of the PubMed, Cochrane Library, EMBASE, Web of Science, and Clinical Trials databases was conducted. Papers published up to 11 February 2021 were considered. The quality of each study was assessed using the modified Consolidated Standards of Reporting Trials checklist for reporting in vitro studies on dental materials. No clinical studies were identified. Six in vitro studies were included, reporting changes in physical and esthetic properties. After performing a methodological quality assessment of the studies, some limitations were identified, the main limitation being the heterogeneous methodology across studies. The evidence resulting from this systematic review did not favor either wet or dry finishing/polishing procedures. There is a clear need for well-designed studies focusing on the comparison of dry/wet finishing/polishing with standard protocols to evaluate the differences among different materials and methods.

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Comparative Evaluation of Quality of Obturation in Primary Teeth Using Zinc Oxide Eugenol and Endoflas under Spiral Computed Tomography: An In Vitro Study

CODS Journal of Dentistry ◽

10.5005/jp-journals-10063-0032 ◽

2017 ◽

Vol 9 (2) ◽

pp. 46-49

Author(s):

Bharath .KP ◽

Poornima P ◽

Nikita Lobo

Keyword(s):

Computed Tomography ◽

Zinc Oxide ◽

Comparative Evaluation ◽

Primary Teeth ◽

In Vitro Study ◽

Spiral Computed Tomography ◽

Vitro Study ◽

Spiral Computed

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Lesion Sterilization Tissue Repair (LSTR) Approach Of Non-Vital Primary Molars With A Chloramphenicol-Tetracycline-ZOE Antibiotic Paste: A Scoping Review

Journal of Clinical Pediatric Dentistry ◽

10.17796/1053-4625-45.6.1 ◽

2021 ◽

Vol 45 (6) ◽

pp. 369-375

Author(s):

Arturo Garrocho-Rangel ◽

Carolina Jalomo-Ávila ◽

Miguel Ángel Rosales-Berber ◽

Amaury Pozos-Guillén

Keyword(s):

Scoping Review ◽

Tissue Repair ◽

Randomized Clinical Trials ◽

Clinical Success ◽

Primary Root ◽

Case Series ◽

In Vitro Studies ◽

Cochrane Library ◽

Primary Molars

The study aimed to perform a systematic scoping review with the need of exploring the actual clinical applications of the chloramphenicol-tetracycline-ZOE antibiotic paste (CTZ) as a lesion sterilization tissue repair (LSTR) therapy agent. Following a scoping framework suggested by Arksey and O’Malley, relevant articles (randomized controlled trials, literature reviews, observational studies, in vitro studies, and clinical case-series reports) published over the last 15 years (in English, Spanish, or Portuguese languages) were identified and retrieved from five internet databases: PubMed, Embase/Ovid, Cochrane Library, Google Scholar, and EBSCO. By title and abstract screening and after removing duplicates, 11 articles were finally included in the present scoping review: five randomized/non-randomized clinical trials, five in vitro studies, and one case-series report. According to the collected information, there were no differences between CTZ paste and conventional pulpectomy, antibiotic pastes, and intracanal filling materials, considering the clinical, radiographic, antimicrobial activity, and periapical tissue biocompatibility outcomes. CTZ has shown excellent rates of clinical success and good radiographic results, with adequate antimicrobial effects; however, its biocompatibility has been put into doubt. Contemporary pediatric dentists should carefully consider the CTZ paste as an alternative endodontic approach for pulpally involved primary molars, with the advantages of being simple and fast, and taking into account the limitations of instrumental pulpectomy such as the microbiological and morphological complexity of primary root canals.

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In Vitro Symbiotic Germination: A Revitalized Heuristic Approach for Orchid Species Conservation

Plants ◽

10.3390/plants9121742 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1742

Author(s):

Galih Chersy Pujasatria ◽

Chihiro Miura ◽

Hironori Kaminaka

Keyword(s):

Natural Habitat ◽

Species Conservation ◽

In Vitro Study ◽

Species Interaction ◽

Life Cycles ◽

Mycorrhizal Symbiosis ◽

Promising Alternative ◽

Symbiotic Germination

As one of the largest families of flowering plants, Orchidaceae is well-known for its high diversity and complex life cycles. Interestingly, such exquisite plants originate from minute seeds, going through challenges to germinate and establish in nature. Alternatively, orchid utilization as an economically important plant gradually decreases its natural population, therefore, driving the need for conservation. As with any conservation attempts, broad knowledge is required, including the species’ interaction with other organisms. All orchids establish mycorrhizal symbiosis with certain lineages of fungi to germinate naturally. Since the whole in situ study is considerably complex, in vitro symbiotic germination study is a promising alternative. It serves as a tool for extensive studies at morphophysiological and molecular levels. In addition, it provides insights before reintroduction into its natural habitat. Here we reviewed how mycorrhiza contributes to orchid lifecycles, methods to conduct in vitro study, and how it can be utilized for conservation needs.

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The Antimicrobial Effect of Cold Atmospheric Plasma against Dental Pathogens—A Systematic Review of In-Vitro Studies

Antibiotics ◽

10.3390/antibiotics10020211 ◽

2021 ◽

Vol 10 (2) ◽

pp. 211

Author(s):

Gert Jungbauer ◽

Dominick Moser ◽

Steffen Müller ◽

Wolfgang Pfister ◽

Anton Sculean ◽

...

Keyword(s):

Randomized Clinical Trials ◽

Assessment Tool ◽

Treatment Time ◽

Antimicrobial Effect ◽

In Vitro Studies ◽

Atmospheric Plasma ◽

Promising Alternative ◽

Cold Atmospheric Plasma

Interest in the application of cold atmospheric plasma (CAP) in the medical field has been increasing. Indications in dentistry are surface modifications and antimicrobial interventions. The antimicrobial effect of CAP is mainly attributed to the generation of reactive oxygen and reactive nitrogen species. The aim of this article is to systematically review the available evidence from in-vitro studies on the antimicrobial effect of CAP on dental pathogens. A database search was performed (PubMed, Embase, Scopus). Data concerning the device parameters, experimental set-ups and microbial cultivation were extracted. The quality of the studies was evaluated using a newly designed assessment tool. 55 studies were included (quality score 31–92%). The reduction factors varied strongly among the publications although clusters could be identified between groups of set pathogen, working gases, and treatment time intervals. A time-dependent increase of the antimicrobial effect was observed throughout the studies. CAP may be a promising alternative for antimicrobial treatment in a clinically feasible application time. The introduced standardized protocol is able to compare the outcome and quality of in-vitro studies. Further studies, including multi-species biofilm models, are needed to specify the application parameters of CAP before CAP should be tested in randomized clinical trials.

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Effect of Cavity Disinfectants on Dentin Bond Strength and Clinical Success of Composite Restorations—A Systematic Review of In Vitro, In Situ and Clinical Studies

International Journal of Molecular Sciences ◽

10.3390/ijms22010353 ◽

2020 ◽

Vol 22 (1) ◽

pp. 353

Author(s):

Ana Coelho ◽

Inês Amaro ◽

Beatriz Rascão ◽

Inês Marcelino ◽

Anabela Paula ◽

...

Keyword(s):

Systematic Review ◽

Bond Strength ◽

Clinical Studies ◽

Antimicrobial Agents ◽

Clinical Success ◽

Scientific Evidence ◽

Cochrane Library ◽

The Cochrane Library

Cavity disinfection becomes an important step before a dental restorative procedure. The disinfection can be obtained cleaning the dental cavity with antimicrobial agents before the use of adhesive systems. The aim of this study was to conduct a systematic review on the effect of different cavity disinfectants on restorations’ adhesion and clinical success. A search was carried out through the Cochrane Library, PubMed, and Web of Science. In vitro and in situ studies reporting results on dentin bond strength tests, and clinical studies published until August 2020, in English, Spanish and Portuguese were included. The methodological quality assessment of the clinical studies was carried out using the Revised Cochrane risk-of-bias tool. Chlorhexidine could preserve adhesion to dentin. EDTA and ethanol had positive results that should be further confirmed. Given the significant lack of scientific evidence, the use of lasers, fluoridated agents, sodium hypochlorite, or other products as cavity disinfectants should be avoided. Chlorhexidine is a safe option for cavity disinfection with adequate preservation of adhesion to dentin. Moreover, future researches should be focused on the efficacy of these disinfectants against cariogenic bacteria and their best application methods.

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