Faculty Opinions recommendation of Dietary restriction in rats and mice: a meta-analysis and review of the evidence for genotype-dependent effects on lifespan.

2016 ◽  
Author(s):  
Valter Longo
Keyword(s):  
Dietary Restriction ◽  
Meta Analysis ◽  
Rats And Mice

scholarly journals Measuring context-dependent memory in rodents: a systematic review and meta-analysis of important variables in the object-in-context task.

2021 ◽  
Author(s):  
Milou Sep ◽  
Marijn Vellinga ◽  
R. Angela Sarabdjitsingh ◽  
Marian Joëls
Keyword(s):  
Systematic Review ◽  
Meta Analysis ◽  
Animal Experiments ◽  
Data Driven ◽  
Evidence Based ◽  
Critical Factors ◽  
Context Dependent ◽  
Set Up ◽  
Rats And Mice ◽  
Learning Trials

Environmental information plays an important role in remembering events. Information about stable aspects of the environment (here referred to as ‘context’) and the event are combined by the hippocampal system and stored as context-dependent memory. In rodents (such as rats and mice), context-dependent memory is often investigated with the object-in-context task. However, the implementation and interpretation of this task varies considerably across studies. This variation hampers the comparison between studies and - for those who design a new experiment or carry out pilot experiments – the estimation of whether observed behavior is within the expected range. Also, it is currently unclear which of the variables critically influence the outcome of the task. To address these issues, we carried out a preregistered systematic review (PROSPERO CRD42020191340) and provide an up-to-date overview of the animal-, task-, and protocol-related variations in the object-in-context task for rodents. Using a data-driven explorative meta-analysis we next identified critical factors influencing the outcome of this task, such as sex, testbox size and the delay between the learning trials. Based on these observations we provide recommendations to create more consensus in the set-up, procedure and interpretation of the object-in-context task for rodents. This could contribute to a more robust and evidence-based design in future animal experiments.

scholarly journals Effect of Streptozotocin on Plasma Insulin Levels of Rats and Mice: A Meta-analysis Study

2015 ◽  
Vol 3 (3) ◽  
pp. 380-383 ◽  
Author(s):  
Burcu Koksal
Keyword(s):  
Plasma Insulin ◽  
Meta Analysis ◽  
Practical Importance ◽  
Diabetic Rats ◽  
Effect Sizes ◽  
Effective Agent ◽  
Insulin Levels ◽  
Plasma Insulin Levels ◽  
The Mean ◽  
Rats And Mice

BACKGROUND: In the studies focusing on diabetic organisms, Streprozotocine (STZ) is a frequently used agent to induce diabetes in rats and mice. However the current studies do not represent practical importance of their statistical findings. For showing practical importance of the differences in plasma insulin levels of diabetic rats and mice induced by STZ, there should be a statistical synthesis regarding statistical findings of the studies. AIM: The purpose of this study is to make a meta-analysis of the studies on the effect of STZ on plasma insulin levels in diabetic rats and mice. MATERIALS AND METHODS: In this study 39 effect sizes (37 studies) about levels of plasma insulin were analyzed by calculating individual effect sizes (d) and mean effect size.RESULTS: The effect sizes were between -13.7 and +65.3 and the mean effect size value (+9.33) represented a large effect indicating that STZ was an effective agent to significantly decrease plasma insulin levels of diabetic rats and mice.CONCLUSION: It can be said that the differences in plasma insulin levels between STZ-applied and no application groups has a practical importance in making animal model of diabetes.

Study of anesthetics for euthanasia in rats and mice: A systematic review and meta-analysis on the impact upon biological outcomes (SAFE-RM)

Life Sciences ◽  
2021 ◽  
Vol 284 ◽  
pp. 119916
Author(s):  
Santiago Alonso Tobar Leitão ◽  
Douglas dos Santos Soares ◽  
Nelson Carvas Junior ◽  
Rafael Zimmer ◽  
Nataniel Floriano Ludwig ◽  
...  
Keyword(s):  
Systematic Review ◽  
Meta Analysis ◽  
Rats And Mice ◽  
The Impact ◽  
Biological Outcomes

Effects of Diet and Diet-Plus-Exercise Programs on Resting Metabolic Rate: A Meta-Analysis

1996 ◽  
Vol 6 (1) ◽  
pp. 41-61 ◽  
Author(s):  
Janice L. Thompson ◽  
Melinda M. Manore ◽  
Jerry R. Thomas
Keyword(s):  
Metabolic Rate ◽  
Dietary Restriction ◽  
Meta Analysis ◽  
Resting Metabolic Rate ◽  
Premenopausal Women ◽  
High Carbohydrate Diet ◽  
Moderate Intensity ◽  
Low Fat ◽  
Carbohydrate Diet ◽  
High Carbohydrate

Studies examining the effects of diet (D) and diet-plus-exercise (DE) programs on resting metabolic rate (RMR) report equivocal results. The purpose of this study was to use meta-analysis to determine if exercise prevents the decrease in RMR observed with dieting. Results from the 22 studies included in this analysis revealed that the majority of studies used female subjects ages 31-45 years, who were fed a relatively low-fat, high-carbohydrate diet of less than 5,023 kJ · day1. The predominant prescribed exercise was aerobic in nature, 31-60 min in duration, performed 4-5 days per week, and of moderate intensity (51-70% of). Contrary to what is reported in narrative reviews, RMR decreased significantly with both D and DE programs, and the drop with D was significantly greater than that with DE. In conclusion, the addition of exercise to dietary restriction appears to prevent some of the decrease in RMR observed in premenopausal women.

scholarly journals Dietary restriction of rodents decreases aging rate without affecting initial mortality rate - a meta-analysis

Aging Cell ◽  
2013 ◽  
Vol 12 (3) ◽  
pp. 410-414 ◽  
Author(s):  
Mirre J. P. Simons ◽  
Wouter Koch ◽  
Simon Verhulst
Keyword(s):  
Mortality Rate ◽  
Dietary Restriction ◽  
Meta Analysis ◽  
Aging Rate

scholarly journals Sex-dependent effects of prenatal food and protein restriction on offspring physiology in rats and mice: systematic review and meta-analyses

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Julian K. Christians ◽  
Haroop K. Shergill ◽  
Arianne Y. K. Albert
Keyword(s):  
Blood Pressure ◽  
Meta Analysis ◽  
Maternal Diet ◽  
Protein Restriction ◽  
Sprague Dawley ◽  
Eligibility Criteria ◽  
Early Life Adversity ◽  
Males And Females ◽  
Meta Analyses ◽  
Rats And Mice

Abstract Background Males and females may experience different effects of early-life adversity on life-long health. One hypothesis is that male foetuses invest more in foetal growth and relatively less in placental growth, and that this makes them susceptible to poor nutrition in utero, particularly if nutrition is reduced part-way through gestation. Objectives Our objectives were to examine whether (1) food and/ or protein restriction in rats and mice has consistent sex-dependent effects, (2) sex-dependency differs between types of outcomes, and (3) males are more severely affected when restriction starts part-way through gestation. Data sources PubMed and Web of Science were searched to identify eligible studies. Study eligibility criteria Eligible studies described controlled experiments that restricted protein or food during gestation in rats or mice, examined physiological traits in offspring from manipulated pregnancies, and tested whether effects differed between males and females. Results Our search identified 292 articles, of which the full texts of 72 were assessed, and 65 were included for further synthesis. A majority (50) used Wistar or Sprague-Dawley rats and so these were the primary focus. Among studies in which maternal diet was restricted for the duration of gestation, no type of trait was consistently more severely affected in one particular sex, although blood pressure was generally increased in both sexes. Meta-analysis found no difference between sexes in the effect of protein restriction throughout gestation on blood pressure. Among studies restricting food in the latter half of gestation only, there were again few consistent sex-dependent effects, although three studies found blood pressure was increased in males only. Meta-analysis found that food restriction in the second half of gestation increased adult blood pressure in both sexes, with a significantly greater effect in males. Birthweight was consistently reduced in both sexes, a result confirmed by meta-analysis. Conclusions We found little support for the hypotheses that males are more affected by food and protein restriction, or that effects are particularly severe if nutrition is reduced part-way through gestation. However, less than half of the studies tested for sex by maternal diet interactions to identify sex-dependent effects. As a result, many reported sex-specific effects may be false positives.

scholarly journals The rodent object-in-context task: A systematic review and meta-analysis of important variables

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0249102
Author(s):  
Milou S. C. Sep ◽  
Marijn Vellinga ◽  
R. Angela Sarabdjitsingh ◽  
Marian Joëls
Keyword(s):  
Systematic Review ◽  
Meta Analysis ◽  
Animal Experiments ◽  
Data Driven ◽  
Evidence Based ◽  
Memory Phase ◽  
Context Dependent ◽  
Set Up ◽  
Rats And Mice ◽  
Learning Trials

Environmental information plays an important role in remembering events. Information about stable aspects of the environment (here referred to as ‘context’) and the event are combined by the hippocampal system and stored as context-dependent memory. In rodents (such as rats and mice), context-dependent memory is often investigated with the object-in-context task. However, the implementation and interpretation of this task varies considerably across studies. This variation hampers the comparison between studies and—for those who design a new experiment or carry out pilot experiments–the estimation of whether observed behavior is within the expected range. Also, it is currently unclear which of the variables critically influence the outcome of the task. To address these issues, we carried out a preregistered systematic review (PROSPERO CRD42020191340) and provide an up-to-date overview of the animal-, task-, and protocol-related variations in the object-in-context task for rodents. Using a data-driven explorative meta-analysis we next identified critical factors influencing the outcome of this task, such as sex, testbox size and the delay between the learning trials. Based on these observations we provide recommendations on sex, strain, prior arousal, context (size, walls, shape, etc.) and timing (habituation, learning, and memory phase) to create more consensus in the set-up, procedure, and interpretation of the object-in-context task for rodents. This could contribute to a more robust and evidence-based design in future animal experiments.

Sign in / Sign up

Export Citation Format

Share Document