Faculty Opinions recommendation of Mechanosensing by the Lamina Protects against Nuclear Rupture, DNA Damage, and Cell-Cycle Arrest.

SummaryWhether cell forces or extracellular matrix (ECM) can impact genome integrity is largely unclear. Here, acute perturbations (~1hr) to actomyosin stress or ECM elasticity cause rapid and reversible changes in lamin-A, DNA damage, and cell cycle. Embryonic hearts, differentiated iPS-cells, and various nonmuscle cell types all show that actomyosin-driven nuclear rupture causes cytoplasmic mis-localization of DNA repair factors and excess DNA damage. Binucleation and micronuclei increase as telomeres shorten, which all favor cell cycle arrest. Deficiencies in lamin-A and repair factors exacerbate these effects, but lamin-A-associated defects are rescued by repair factor overexpression and by contractility modulators in clinical trials. Contractile cells on stiff ECM normally exhibit low phosphorylation and slow degradation of lamin-A by matrix-metalloprotease-2 (MMP2), and inhibition of this lamin-A turnover and also actomyosin contractility is seen to minimize DNA damage. Lamin-A is thus stress-stabilized to mechano-protect the genome.

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Design, Synthesis and Pharmacological Evaluation of Three Novel Dehydroabietyl Piperazine Dithiocarbamate Ruthenium (II) Polypyridyl Complexes as Potential Antitumor Agents: DNA Damage, Cell Cycle Arrest and Apoptosis Induction

Molecules ◽

10.3390/molecules26051453 ◽

2021 ◽

Vol 26 (5) ◽

pp. 1453

Author(s):

Haoran Wang ◽

Jianhua Wei ◽

Hong Jiang ◽

Ye Zhang ◽

Caina Jiang ◽

...

Keyword(s):

Cell Cycle ◽

Dna Damage ◽

Cell Cycle Arrest ◽

Antitumor Agents ◽

Mechanistic Study ◽

Ros Generation ◽

Polypyridyl Complexes ◽

Expression Levels ◽

Cycle Arrest ◽

Study Results

The use of cisplatin is severely limited by its toxic side-effects, which has spurred chemists to employ different strategies in the development of new metal-based anticancer agents. Here, three novel dehydroabietyl piperazine dithiocarbamate ruthenium (II) polypyridyl complexes (6a–6c) were synthesized as antitumor agents. Compounds 6a and 6c exhibited better in vitro antiproliferative activity against seven tumor cell lines than cisplatin, they displayed no evident resistance in the cisplatin-resistant cell line A549/DPP. Importantly, 6a effectively inhibited tumor growth in the T-24 xenograft mouse model in comparison with cisplatin. Gel electrophoresis assay indicated that DNA was the potential targets of 6a and 6c, and the upregulation of p-H2AX confirmed this result. Cell cycle arrest studies demonstrated that 6a and 6c arrested the cell cycle at G1 phase, accompanied by the upregulation of the expression levels of the antioncogene p27 and the down-regulation of the expression levels of cyclin E. In addition, 6a and 6c caused the apoptosis of tumor cells along with the upregulation of the expression of Bax, caspase-9, cytochrome c, intracellular Ca2+ release, reactive oxygen species (ROS) generation and the downregulation of Bcl-2. These mechanistic study results suggested that 6a and 6c exerted their antitumor activity by inducing DNA damage, and consequently causing G1 stage arrest and the induction of apoptosis.

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ERK activation mediates cell cycle arrest and apoptosis after DNA damage independently of p53.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(20)84970-9 ◽

2002 ◽

Vol 277 (23) ◽

pp. 21110 ◽

Cited By ~ 1

Author(s):

Damu Tang ◽

Dongcheng Wu ◽

Atsushi Hirao ◽

Jill M. Lahti ◽

Lieqi Liu ◽

...

Keyword(s):

Cell Cycle ◽

Dna Damage ◽

Cell Cycle Arrest ◽

Erk Activation ◽

Cycle Arrest

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P28-10 Cisplatin induces DNA damage associated with cell cycle arrest and apoptosis in PC9 cells

Annals of Oncology ◽

10.1016/j.annonc.2021.05.736 ◽

2021 ◽

Vol 32 ◽

pp. S346

Author(s):

Md Mohiuddin ◽

Hideharu Kimura ◽

Takashi Sone ◽

Hiroki Matsuoka ◽

Keigo Saeki ◽

...

Keyword(s):

Cell Cycle ◽

Dna Damage ◽

Cell Cycle Arrest ◽

Cycle Arrest

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Aqueous Extracts of the Edible Gracilaria tenuistipitata are Protective Against H2O2-Induced DNA Damage, Growth Inhibition, and Cell Cycle Arrest

Molecules ◽

10.3390/molecules17067241 ◽

2012 ◽

Vol 17 (6) ◽

pp. 7241-7254 ◽

Cited By ~ 38

Author(s):

Jing-Iong Yang ◽

Chi-Chen Yeh ◽

Jin-Ching Lee ◽

Szu-Cheng Yi ◽

Hurng-Wern Huang ◽

...

Keyword(s):

Cell Cycle ◽

Dna Damage ◽

Growth Inhibition ◽

Cell Cycle Arrest ◽

Aqueous Extracts ◽

Damage Growth ◽

Gracilaria Tenuistipitata ◽

Cycle Arrest

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Mollugin induced oxidative DNA damage via up-regulating ROS that caused cell cycle arrest in hepatoma cells

Chemico-Biological Interactions ◽

10.1016/j.cbi.2022.109805 ◽

2022 ◽

pp. 109805

Author(s):

Xin-ge Ke ◽

Yi-yi Xiong ◽

Bing Yu ◽

Chong Yuan ◽

Peng-yu Chen ◽

...

Keyword(s):

Cell Cycle ◽

Dna Damage ◽

Cell Cycle Arrest ◽

Oxidative Dna Damage ◽

Hepatoma Cells ◽

Cycle Arrest

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Octadecyloxyethyl Adefovir Exhibits Potent in vitro and in vivo Cytotoxic Activity and Has Synergistic Effects with Ara-C in Acute Myeloid Leukemia

Chemotherapy ◽

10.1159/000491705 ◽

2018 ◽

Vol 63 (4) ◽

pp. 225-237 ◽

Cited By ~ 1

Author(s):

Haytham Khoury ◽

Ruijuan He ◽

Aaron Schimmer ◽

James R. Beadle ◽

Karl Y. Hostetler ◽

...

Keyword(s):

Cell Cycle ◽

Dna Damage ◽

Acute Myeloid Leukemia ◽

Cell Cycle Arrest ◽

Clinical Benefit ◽

Myeloid Leukemia ◽

Mouse Bone Marrow ◽

Medical Needs ◽

Cycle Arrest ◽

Acute Myeloid

Acute myeloid leukemia (AML) continues to be a deadly disease, with only 50–70% of patients achieving complete remission and less than 30% of adults having sustained long-term remissions. In order to address these unmet medical needs, we carried out a high-throughput screen of an in-house library of on- and off-patent drugs with the OCI/AML-2 cell line. Through this screen, we discovered adefovir dipivoxil (adefovir-DP) as being active against human AML. In addition to adefovir-DP, there are second-generation formulations of adefovir, including octadecyloxyethyl adefovir (ODE-adefovir) and hexadecyloxypropyl adefovir (HDP-adefovir), which were designed to overcome the pharmacokinetic problems of the parent compound adefovir. Given the known clinical benefit of nucleoside analogs for the treatment of AML, we undertook studies to evaluate the potential benefit of adefovir-based molecules. In AML cell lines and patient samples, adefovir-DP and ODE-adefovir were highly potent, whereas HDP-adefovir was significantly less active. Interestingly, ODE-adefovir was remarkably less toxic than adefovir-DP towards normal hematopoietic cells. In addition, ODE-adefovir at a dose of 15 mg/kg/day showed potent activity against human AML in a NOD/SCID mouse model, with a reduction of human leukemia in mouse bone marrow of > 40% in all mice tested within 20 days of treatment. Based on its chemical structure, we hypothesized that the cytotoxicity of ODE-adefovir toward AML was through cell cycle arrest and DNA damage. Indeed, ODE-adefovir treatment induced cell cycle arrest in the S phase and increased levels of pH2Ax, indicating the induction of DNA damage. Furthermore, there was an increase in phospho-p53, transactivation of proapoptotic genes and activation of the intrinsic apoptotic pathway. Subsequent investigation unveiled strong synergism between ODE-adefovir and ara-C, making their coadministration of potential clinical benefit. Expression of MRP4, a nucleoside transporter, appeared to influence the response of AML cells to ODE-adefovir, as its inhibition potentiated ODE-adefovir killing. Taken together, our findings indicate that clinical development of ODE-adefovir or related compounds for the treatment of AML is warranted.

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Single v dual knockdowns of the chromatin remodeling ATPase, SNF2L and its isoform, SNF2LT have similar effects on DNA damage but opposite effects on the DNA damage response, cell cycle arrest and apoptosis

The FASEB Journal ◽

10.1096/fasebj.24.1_supplement.421.2 ◽

2010 ◽

Vol 24 (S1) ◽

Author(s):

Sanford H Barsky ◽

Yi Xiao ◽

Kurtis Yearsley ◽

Yin Ye

Keyword(s):

Cell Cycle ◽

Dna Damage ◽

Cell Cycle Arrest ◽

Chromatin Remodeling ◽

Dna Damage Response ◽

Cycle Arrest ◽

Damage Response

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RUNX Family Participates in the Regulation of p53-Dependent DNA Damage Response

International Journal of Genomics ◽

10.1155/2013/271347 ◽

2013 ◽

Vol 2013 ◽

pp. 1-12 ◽

Cited By ~ 22

Author(s):

Toshinori Ozaki ◽

Akira Nakagawara ◽

Hiroki Nagase

Keyword(s):

Cell Cycle ◽

Dna Damage ◽

Cell Death ◽

Cell Cycle Arrest ◽

Dna Damage Response ◽

Apoptotic Cell ◽

Genetic Alterations ◽

Apoptotic Cell Death ◽

Cycle Arrest ◽

Damage Response

A proper DNA damage response (DDR), which monitors and maintains the genomic integrity, has been considered to be a critical barrier against genetic alterations to prevent tumor initiation and progression. The representative tumor suppressor p53 plays an important role in the regulation of DNA damage response. When cells receive DNA damage, p53 is quickly activated and induces cell cycle arrest and/or apoptotic cell death through transactivating its target genes implicated in the promotion of cell cycle arrest and/or apoptotic cell death such asp21WAF1,BAX, andPUMA. Accumulating evidence strongly suggests that DNA damage-mediated activation as well as induction of p53 is regulated by posttranslational modifications and also by protein-protein interaction. Loss of p53 activity confers growth advantage and ensures survival in cancer cells by inhibiting apoptotic response required for tumor suppression. RUNX family, which is composed of RUNX1, RUNX2, and RUNX3, is a sequence-specific transcription factor and is closely involved in a variety of cellular processes including development, differentiation, and/or tumorigenesis. In this review, we describe a background of p53 and a functional collaboration between p53 and RUNX family in response to DNA damage.

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