Faculty Opinions recommendation of There's more to death than life: non-catalytic functions in kinase and pseudokinase signaling.

2021 ◽  
Author(s):  
John Lowe
Keyword(s):  
Catalytic Functions

scholarly journals Integration of photocatalytic and dark-operating catalytic biomimetic transformations through DNA-based constitutional dynamic networks

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Chen Wang ◽  
Michael P. O’Hagan ◽  
Ehud Neumann ◽  
Rachel Nechushtai ◽  
Itamar Willner
Keyword(s):  
Nucleic Acid ◽  
Energy Harvesting ◽  
Information Transfer ◽  
Dynamic Networks ◽  
Research Direction ◽  
Cellular Biology ◽  
Dynamic Feedback ◽  
Dynamic Coupling ◽  
Metabolic Processes ◽  
Catalytic Functions

AbstractNucleic acid-based constitutional dynamic networks (CDNs) have recently emerged as versatile tools to control a variety of catalytic processes. A key challenge in the application of these systems is achieving intercommunication between different CDNs to mimic the complex interlinked networks found in cellular biology. In particular, the possibility to interface photochemical ‘energy-harvesting’ processes with dark-operating ‘metabolic’ processes, in a similar way to plants, represents an up to now unexplored yet enticing research direction. The present study introduces two CDNs that allow the intercommunication of photocatalytic and dark-operating catalytic functions mediated by environmental components that facilitate the dynamic coupling of the networks. The dynamic feedback-driven intercommunication of the networks is accomplished via information transfer between the two CDNs effected by hairpin fuel strands in the environment of the system, leading to the coupling of the photochemical and dark-operating modules.

scholarly journals Restoration of catalytic functions in Cre recombinase mutants by electrostatic compensation between active site and DNA substrate

2010 ◽  
Vol 38 (19) ◽  
pp. 6589-6601 ◽  
Author(s):  
A. H. Kachroo ◽  
C.-H. Ma ◽  
P. A. Rowley ◽  
A. D. Maciaszek ◽  
P. Guga ◽  
...  
Keyword(s):  
Active Site ◽  
Cre Recombinase ◽  
Catalytic Functions ◽  
Dna Substrate

Core-Shell Nanoparticle Clusters Enable Synergistic Integration of Plasmonic and Catalytic Functions in a Single Platform

Small ◽  
2017 ◽  
Vol 13 (43) ◽  
pp. 1701633 ◽  
Author(s):  
Seunghoon Lee ◽  
Younghyun Wy ◽  
Young Wook Lee ◽  
Kyungrok Ham ◽  
Sang Woo Han
Keyword(s):  
Core Shell ◽  
Nanoparticle Clusters ◽  
Catalytic Functions

A mesoporous Pt-SBA-15 nano architecture with catalytic functions on oxidation of CO

2010 ◽  
Vol 18 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Chao Yang ◽  
Zhenzhen Wang ◽  
Xiaochong Zhou ◽  
Xike Tian ◽  
Zhenbang Pi ◽  
...  
Keyword(s):  
Oxidation Of Co ◽  
Catalytic Functions

scholarly journals Expression and Isolation of N-Terminal Truncated Human Recombinant Renalase in Prokaryotic Cells

2021 ◽  
Vol 4 (3) ◽  
pp. e00158
Author(s):  
V.I. Fedchenko ◽  
A.A. Kaloshin ◽  
S.A. Kaloshina ◽  
A.E. Medvedev
Keyword(s):  
Recombinant Protein ◽  
Signal Peptide ◽  
Extracellular Space ◽  
Inclusion Bodies ◽  
High Concentration ◽  
Genetic Construct ◽  
E Coli ◽  
Insoluble Form ◽  
Guanidine Chloride ◽  
Catalytic Functions

Renalase (RNLS) is a flavoproteinin which its N-terminal peptide (residues 1-17) has several important functions. In cells, it participates in the formation of the so-called Rossmanfold (residues 2-35), needed for «accommodation» of the FAD cofactor and for performing the catalytic functions of RNLS as a FAD-dependent oxidoreductase (EC 1.6.3.5). RNLS secretion into the extracellular space is accompanied by cleavage of this peptide. The resultant truncated extracellular RNLS cannot bind FAD and therefore performs various noncatalytic functions. In this work, we have performed expression the genetic construct encoding RNLS lacking its N-terminal signal peptide (tRNLS) in E. coli Rosetta (DE3) cells. The recombinant protein was accumulated in inclusion bodies in an insoluble form, which could be solubilized in the presence of a high concentration of urea or guanidine chloride. In contrast to full-length RNLS, which was effectively solubilized in the presence of 8 M urea, tRNLS was preferentially solubilized in the presence of 6 M guanidine chloride.

scholarly journals Application of enzyme engineering in pharmacy

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Qiaole Li ◽  
Yanhong Li ◽  
Haoran Niu
Keyword(s):  
High Efficiency ◽  
Development Trend ◽  
Enzyme Engineering ◽  
Reaction Conditions ◽  
Important Method ◽  
Reaction Specificity ◽  
Prevention And Treatment ◽  
Modern Biotechnology ◽  
Catalytic Functions ◽  
The Development Trend

Enzyme engineering is an important part of modern biotechnology. Due to its high reaction specificity, high efficiency, mild reaction conditions, and low pollution, it is also an important method widely used in the pharmaceutical field. The application of enzymes in medicine is diverse, such as: diagnosis, prevention and treatment of diseases with enzymes, manufacture of various drugs with enzymes, etc., mainly through manual operations, to obtain enzymes required by the pharmaceutical industry, and through various means Enzymes perform their catalytic functions. This article mainly introduces the application of enzyme engineering in the pharmaceutical field, and also prospects the development trend of enzyme engineering in the pharmaceutical field.

Role of Ca2+ in the Substrate Binding and Catalytic Functions of Snake Venom Phospholipases A21

1989 ◽  
Vol 106 (3) ◽  
pp. 518-527 ◽  
Author(s):  
Keizo Teshima ◽  
Yutaka Kitagawa ◽  
Yuji Samejima ◽  
Saju Kawauchi ◽  
Shinobu Fujii ◽  
...  
Keyword(s):  
Snake Venom ◽  
Substrate Binding ◽  
Catalytic Functions
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