scholarly journals Culture of Bacterium Bacillus subtilis as Degradation Agent in Attempt of Sea Water Remediation Contaminated By Petroleum

2020 ◽  
Vol 3 (1) ◽  
pp. 53 ◽  
Author(s):  
Adi Setyo Purnomo ◽  
Hamdan Dwi Rizqi ◽  
Lia Harmelia
Keyword(s):  
Bacillus Subtilis ◽  
Crude Oil ◽  
Oil Spill ◽  
Oil Spills ◽  
Sea Water ◽  
Water Remediation ◽  
Gas Chromatography Mass Spectrometry ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus ◽  
Simulated Seawater

Crude oil spills pose a serious threat to the marine environment. This is due to crude oil, in large part, is composed of aromatic, aliphatic, and alicyclic hydrocarbons which are toxic, carcinogenic, and mutagenic to the marine life. However, the degradation of crude oil spills with bacteria in simulated seawater media is rarely reported. In this study, oil spill in the seawater, especially petroleum, had been successfully degraded by bacterium culture Bacillus subtilis in simulated seawater under 7 and 14 days incubation.  Simulated seawater had synthesized based on ASTM D1141-98 for Standard Practice for the Preparation of Substitute Ocean Water. The petroleum recovery was analyzed using Gas chromatography-mass spectrometry.  In this research, the optimum recovery value of crude oil degradation by the bacterial culture obtained by octadecadienoic acid compound gave a percentage recovery of 8.20% and 8.87% after 7 and 14 days of incubation, respectively. This result indicated that the B. subtilis culture has the ability to degrade crude oil spill in simulated sea water.

scholarly journals Biosurfactant and Degradative Enzymes Mediated Crude Oil Degradation by Bacterium Bacillus subtilis A1

2017 ◽  
Vol 8 ◽  
Author(s):  
Punniyakotti Parthipan ◽  
Elumalai Preetham ◽  
Laura L. Machuca ◽  
Pattanathu K. S. M. Rahman ◽  
Kadarkarai Murugan ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Crude Oil ◽  
Oil Degradation ◽  
Crude Oil Degradation ◽  
Degradative Enzymes ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

scholarly journals Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Naomi Shimokawa-Chiba ◽  
Claudia Müller ◽  
Keigo Fujiwara ◽  
Bertrand Beckert ◽  
Koreaki Ito ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Stop Codon ◽  
Release Factor ◽  
Positive Bacterium ◽  
Gram Positive ◽  
E Coli ◽  
Cryo Electron Microscopy ◽  
Dead End ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

AbstractRescue of the ribosomes from dead-end translation complexes, such as those on truncated (non-stop) mRNA, is essential for the cell. Whereas bacteria use trans-translation for ribosome rescue, some Gram-negative species possess alternative and release factor (RF)-dependent rescue factors, which enable an RF to catalyze stop-codon-independent polypeptide release. We now discover that the Gram-positive Bacillus subtilis has an evolutionarily distinct ribosome rescue factor named BrfA. Genetic analysis shows that B. subtilis requires the function of either trans-translation or BrfA for growth, even in the absence of proteotoxic stresses. Biochemical and cryo-electron microscopy (cryo-EM) characterization demonstrates that BrfA binds to non-stop stalled ribosomes, recruits homologous RF2, but not RF1, and induces its transition into an open active conformation. Although BrfA is distinct from E. coli ArfA, they use convergent strategies in terms of mode of action and expression regulation, indicating that many bacteria may have evolved as yet unidentified ribosome rescue systems.

Glycosylated Methoxy-Macrolactins from a Marine Sediment Bacterium Bacillus subtilis

Heterocycles ◽  
2013 ◽  
Vol 87 (2) ◽  
pp. 307 ◽  
Author(s):  
Hee Jae Shin ◽  
Fakir Shahidullah Tareq ◽  
Ji Hye Kim ◽  
Min Ah Lee ◽  
Hyi-Seung Lee ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Marine Sediment ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

Uncoupling and toxic action of alkyltriphenylphosphonium cations on mitochondria and the bacterium Bacillus subtilis as a function of alkyl chain length

2015 ◽  
Vol 80 (12) ◽  
pp. 1589-1597 ◽  
Author(s):  
L. S. Khailova ◽  
P. A. Nazarov ◽  
N. V. Sumbatyan ◽  
G. A. Korshunova ◽  
T. I. Rokitskaya ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Chain Length ◽  
Alkyl Chain ◽  
Alkyl Chain Length ◽  
Toxic Action ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

Fractionation of an Aquatic Fulvic Acid upon Adsorption to the Bacterium,Bacillus subtilis

2004 ◽  
Vol 21 (2) ◽  
pp. 69-78 ◽  
Author(s):  
Patricia A. Maurice ◽  
Maciej Manecki ◽  
Jeremy B. Fein ◽  
Jennifer Schaefer
Keyword(s):  
Bacillus Subtilis ◽  
Fulvic Acid ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

scholarly journals UJI AKTIVITAS ANTIBAKTERI SPRAY BAU KAKI EKSTRAK KULIT JERUK NIPIS (Citrus aurantifolia) DENGAN VARIASI Gelling agent TERHADAP BAKTERI Bacillus subtilis

2021 ◽  
Vol 9 (1) ◽  
pp. 18-26
Author(s):  
Ade Maria Ulfa ◽  
◽  
Nofita Nofita ◽  
Bangun Saras Sandi ◽  
◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacterial Infections ◽  
Inhibition Zone ◽  
Gelling Agent ◽  
Apocrine Glands ◽  
Citrus Aurantifolia ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus ◽  
Lime Peel ◽  
Peel Extract

ABSTRACT Sweat is produced by the apocrine glands, if infected by bacteria that play a role in the decay process will certainly produce foot odor. Some of the bacteria that cause, including Staphylococcus epidermis, Corynebacterium acne and there is one bacterium that causes pungent foot odor that is Bacillus subtilis. Bacillus subtilis enzyme leucine dehydrogenase produced the highest, resulting in isovaleric acid foot odor. Lime peel (Citrus aurantifolia) has the potential to be developed for the antibacterial active ingredient of foot odor contained in tannins, alkaloids and flavonoids. Spray can be effective for inhibition of feet due to water fleas or bacterial infections. The purpose of this research is to test the inhibitory zone of the preparation of foot odor spray ethanol extract of lime peel (Citrus aurantifolia) with variations of gelling agent. Bacterial inhibition zone testing on extracts of lime peel spray preparations using the disc method. This test was carried out on spray with extract concentration of 0% extract base carbopol, 0% extract base HPMC, 0.2% extract base carbopol, 0.2% extract base HPMC, 0.4% extract base carbopol, 0.4% extract base HPMC and positive control with an average inhibition zone of 9,13 mm, 9,12 mm, 11,86 mm, 11,29 mm, 13,17 mm, 12,30 mm, 8,13 mm against the bacterium Bacillus subtilis. Antibacterial test results were analyzed using ONE WAY ANOVA, the results of statistical analysis on the preparation of lime peel extract showed a significant inhibition zone difference of 0.000 (P = <0.05) between all concentrations. Lime peel extract spray is effective in inhibiting the bacterium Bacillus subtilis. Key words: Sweat of foot odor, Lime skin (Citrus aurantifolia), Spray, bacteri Bacillus subtilis

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