scholarly journals Testicular structure and spermatogenesis of short mackerel, Rastrelliger brachysoma (Bleeker, 1851) in Upper Gulf of Thailand

2018 ◽  
pp. 30-43 ◽  
Author(s):  
Sinlapachai Senarat ◽  
Wannee Jiraungkoorskul ◽  
Jes Kettratad
Keyword(s):  
Chromatin Condensation ◽  
Primary Spermatocyte ◽  
Ultrastructural Level ◽  
Mitotic Division ◽  
Microscopic Level ◽  
Basal Bodies ◽  
Light Microscopic Level ◽  
Secondary Spermatocyte ◽  
Late Stages

Testicular structure and spermatogenesis of short mackerel, Rastrelliger brachysoma (Bleeker, 1851) (Teleostei: Scombridae) was first investigated. The testicular parenchyma was a lobular organ, which was classified as an unrestricted spermatogonial type. The classification of spermatogenetic stage could be classified into six stages based on the pattern of chromatin condensation and other characterizations at the light microscopic level. These six stages included the primary and secondary spermatogonium, primary and secondary spermatocyte, spermatid and spermatozoon. The spermatogenesis could also be classified into another four stages based on the nuclear and cytoplasmic characterizations at the ultrastructural level. Spermatogonium was the early germ cell. It underwent a series of mitotic division to reach the primary spermatocyte. Secondary spermatocyte was shown as the heterochromatin surrounding the nuclear membrane, which was rarely seen within seminiferous lobules. Stages during the spermatids differentiation comprised of the early, intermediate and late stages which are under the degree/change of chromatin condensation. Finally, the spermatozoon was revealed as the aquasperm primitive type. It was composed of an oval head without an acrosome, a short mid-piece consisting of two basal bodies (proximal and distal centrioles) and a long flagella tail without lateral fins. The axonemes of classical form with 9+2 microtubules were presented in the flagellum.

scholarly journals Spermatogenesis and spermiogenisis in the testes of local Iraqi breed cat (Felis catus)

2012 ◽  
Vol 36 (0E) ◽  
pp. 248-253
Author(s):  
AL-Samarrae N. S.
Keyword(s):  
Germ Cells ◽  
Sertoli Cells ◽  
Chromatin Condensation ◽  
Primary Spermatocyte ◽  
Type I ◽  
Nuclear Chromatin ◽  
Spermatogenic Cells ◽  
Type B ◽  
Secondary Spermatocyte ◽  
Spherical Nuclei

The seminiferous epithelium of the testes of cat consists of two groups of cells; Spermatogenic cells and Sertoli cells. The interstitial areas are filled with Leydic cells, blood and lymph vessels, and connective tissue. Germ cells in the Spermatogenic process of the testis of cat can be classified into ten steps, based on the pattern degree of nuclear chromatin condensation. Primary spermatogonia contain large spherical nuclei with mostly euchromatin. Spermatogonia proliferate to give rise to spermatogonia type –A; Intermediate or type-I spermatogonia, and spermatogonia type-B. Type–B spermatogonia yield primary spermatocyte at the end of mitosis. The primary spermatocyte is transformed into secondary spermatocyte during meiosis I. These cells are converted into spermatid during meiosis II. Metamorphosis of spermatids shows: Golgi step, Cap step, Acrosomal step, Maturation step.

scholarly journals Development of a simple embedding procedure allowing immunocytochemical localization at the ultrastructural level.

1988 ◽  
Vol 36 (12) ◽  
pp. 1579-1582 ◽  
Author(s):  
G Escolar ◽  
J J Sauk ◽  
M L Bravo ◽  
M Krumwiede ◽  
J G White
Keyword(s):  
Low Temperature ◽  
Light Microscopy ◽  
Uv Light ◽  
Ultrastructural Level ◽  
Microscopic Level ◽  
Water Soluble ◽  
Immunocytochemical Localization ◽  
Protein Antigens ◽  
Light Microscopic Level ◽  
Thin Sections

Immunobed solution A is a water-soluble acrylic compound recently developed for immunocytochemical localization at the light microscopic level. In this study, we combined it with methyl methacrylate (MMA) to achieve sufficient hardness to obtain ultra-thin sections. Samples of platelets were dehydrated and embedded in the water-soluble acrylic mixture (WSAM). The embedding process was carried out at 4 degrees C and final polymerization was induced with either chemical (benzoyl peroxide) or physical (UV light) catalysts. Tubulin was localized at the ultrastructural level in sections embedded according to these two methods. Results were compared with those obtained in platelets processed in Lowicryl. Dehydration and embedding with the WSAM yielded a preservation of antigenicity similar to that obtained in Lowicryl. The new procedure benefits from the low temperature achieved during polymerization, providing good ultrastructural morphology and immunolocalization of protein antigens with the simplicity of a routine embedding procedure for light microscopy.

Müller cells in the retina of the cane toad, Bufo marinus, express neuropeptide Y-like immunoreactivity

1996 ◽  
Vol 13 (3) ◽  
pp. 501-508 ◽  
Author(s):  
Bao-Song Zhu ◽  
Ian Gibbins
Keyword(s):  
Amacrine Cell ◽  
Müller Cells ◽  
Plexiform Layer ◽  
Amacrine Cells ◽  
Ultrastructural Level ◽  
Microscopic Level ◽  
Inner Plexiform Layer ◽  
Muller Cells ◽  
Light Microscopic Level ◽  
Cane Toad

AbstractWe have used light- and electron-microscopic immunohistochemistry to identify the presence of immunoreactivity to neuropeptide Y (NPY) within Müller cells in the retina of the cane toad, Bufo marinus. Müller cells containing NPY-like immunoreactivity (NPY-LI) were identified at the light-microscopic level by the coexistence with immunoreactivity to glial fibrillary acidic protein (GFAP) and at the ultrastructural level by their characteristic relationship to neuron cell bodies and processes. At the light-microscopic level, those cells which contained both NPY-LI and GFAP-LI usually had small cell bodies in the inner nuclear layer, while those cells which contained only NPY-LI were identified as large and small amacrine cells. The radially oriented primary processes in the inner plexiform layer and the vitreal end feet of GFAP-LI Müller cells also expressed NPY-LI. At the ultrastructural level, thin lamellar processes of Müller cells with NPY-LI enclosed some amacrine cell bodies in the inner nuclear layer and amacrine cell dendrites in the inner plexiform layer. These observations suggest that NPY-LI is localized in Müller cells in addition to two types of amacrine cells previously identified in the Bufo retina. This study provides the first evidence that glial elements in the vertebrate retina express NPY-LI.

scholarly journals Radioimmunocytochemistry using a tritiated goat anti-rabbit second antibody.

1984 ◽  
Vol 32 (7) ◽  
pp. 778-782 ◽  
Author(s):  
E J Glazer ◽  
J Ramachandran ◽  
A I Basbaum
Keyword(s):  
Substance P ◽  
Epoxy Resin ◽  
Immunoglobulin G ◽  
Spinal Dorsal Horn ◽  
Ultrastructural Level ◽  
Microscopic Level ◽  
Light Microscopic Level ◽  
Spinal Dorsal ◽  
Double Label ◽  
Polyclonal Antisera

Affinity-purified goat anti-rabbit immunoglobulin G (GAR) was conjugated with (3H)-propionyl succinimidate and used to localize substance P (SP), enkephalin (ENK), and serotonin immunoreactive sites in the spinal dorsal horn and medulla of the rat and cat. Autoradiographic localization was demonstrated on paraffin, frozen, Vibratome, and 2 micron plastic sections. The latter were obtained from radiolabeled Vibratome sections that were embedded in epoxy resin. The distribution of SP, ENK, and serotonin demonstrated by radioimmunocytochemistry was comparable to that observed on semiadjacent sections using peroxidase-antiperoxidase (PAP) immunocytochemistry. The autoradiograms, however, were generated using primary antibody concentrations up to five times more dilute than concentrations used for the PAP procedure. Indirect radioimmunocytochemistry using a (3H) anti-immunoglobulin G second antibody can be used to localize a variety of monoclonal and polyclonal antisera. It is quantifiable at the light microscopic level and can be potentially used with peroxidase histochemistry to double label immunoreactive structures at the ultrastructural level.

scholarly journals Visualization of centrioles and basal bodies by fluorescent staining with nonimmune rabbit sera.

1978 ◽  
Vol 79 (2) ◽  
pp. 526-532 ◽  
Author(s):  
J A Connolly ◽  
V I Kalnins
Keyword(s):  
Indirect Immunofluorescence ◽  
Cell Types ◽  
Microscopic Level ◽  
Positive Staining ◽  
Basal Bodies ◽  
Fluorescent Staining ◽  
Mitotic Spindles ◽  
Light Microscopic Level ◽  
Large Populations ◽  
Brain Tubulin

Several sera from nonimmunized rabbits have been found which stain centrioles and basal bodies by indirect immunofluorescence in a wide variety of cell types. So far, approximately 10% of the rabbit sera that we have examined gave strong positive staining of centrioles and basal bodies. Cytoplasmic networks, mitotic spindles, and ciliary axonemes, however, remain unstained. This specific fluorescent staining of centrioles and basal bodies could not be abolished by absorption of sera with purified brain tubulin. This technique is superior to previous methods for the visualization of basal bodies and centrioles at the light microscopic level and should be useful for rapid and convenient detection of these organelles in large populations of cells.

scholarly journals Immunocytologic analyses of 10 nm intermediate filaments in the nervous system of Myxicola.

1980 ◽  
Vol 28 (12) ◽  
pp. 1312-1318 ◽  
Author(s):  
L F Eng ◽  
R J Lasek ◽  
J W Bigbee ◽  
D L Eng
Keyword(s):  
Nervous System ◽  
Glial Cells ◽  
Staining Pattern ◽  
Ultrastructural Level ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Antigenic Determinants ◽  
Light Microscopic Level ◽  
Neurofilament Protein ◽  
Electron Microscopic

Antibodies prepared in rabbits against Myxicola infundibulum neurofilaments have been employed to stain neurofilaments immunohistochemically in intact Myxicola infundibulum nervous tissue. Paraffin-embedded and frozen sections (5--6 mu) were examined at the light microscopic level with Sternberger's peroxidase-antiperoxidase method, and Vibratome (20--40 mu) sections were studied at the ultrastructural level with Nakane's conjugated peroxidase method. The neurofilament antibody stained only neurons and axons at the light microscopic level. The staining pattern at the electron microscopic level corresponded to the neurofilaments within axons and neurons. Glial cells, which surround the axons, contain large bundles of filaments that resemble astrocytic filaments in mammalian astrocytes. These filaments do not stain with the anti-neurofilament antibody. Neurons, neurofilaments, glial cells, glial filaments, and nonnervous tissue showed no peroxidase staining when specific antiserum absorbed with neurofilaments was used. These structures were also unstained when antiserum to the glial fibrillary acidic protein of mammalian central nervous system astrocytes was substituted for the neurofilament antiserum. Therefore, in Myxicola infundibulum, the antigenic determinants of the neurofilament protein, as recognized immunohistochemically by anti-neurofilament protein antibodies, are not shared with those of glial filaments.

Diagnostic Value of Electron Microscopy in Soft Tissue Tumors

1970 ◽  
Vol 28 ◽  
pp. 220-221
Author(s):  
Gerald Fine ◽  
Azorides R. Morales
Keyword(s):  
Cardiac Myxoma ◽  
Osteogenic Sarcoma ◽  
Diagnostic Value ◽  
Soft Tissue Tumors ◽  
Amelanotic Melanoma ◽  
Growth Patterns ◽  
Light Microscopic Level ◽  
Mesenchymal Tumors ◽  
Good Agreement

For years the separation of carcinoma and sarcoma and the subclassification of sarcomas has been based on the appearance of the tumor cells and their microscopic growth pattern and information derived from certain histochemical and special stains. Although this method of study has produced good agreement among pathologists in the separation of carcinoma from sarcoma, it has given less uniform results in the subclassification of sarcomas. There remain examples of neoplasms of different histogenesis, the classification of which is questionable because of similar cytologic and growth patterns at the light microscopic level; i.e. amelanotic melanoma versus carcinoma and occasionally sarcoma, sarcomas with an epithelial pattern of growth simulating carcinoma, histologically similar mesenchymal tumors of different histogenesis (histiocytoma versus rhabdomyosarcoma, lytic osteogenic sarcoma versus rhabdomyosarcoma), and myxomatous mesenchymal tumors of diverse histogenesis (myxoid rhabdo and liposarcomas, cardiac myxoma, myxoid neurofibroma, etc.)

Ultrastructural Investigation of Spontaneous Pituitary Adenomas in Aging Sprague-Dawley Rats

1982 ◽  
Vol 40 ◽  
pp. 216-217
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Microscopic Study ◽  
Pituitary Adenomas ◽  
Wistar Rats ◽  
Microscopic Level ◽  
Sprague Dawley ◽  
Prolactin Cells ◽  
Light Microscopic Level ◽  
Ultrastructural Investigation ◽  
Sprague Dawley Rats ◽  
Long Evans

Investigation of the spontaneous pituitary adenomas in rat have been limited mainly to light microscopic study. Furth et al. (1973) described them as chromophobic, secreting prolactin. Kovacs et al. (1977) in an ul trastructural investigation of adenomas of old female Long-Evans rats, found that they were composed of prolactin cells. Berkvens et al. (1980) using immunocytochemistry at the light microscopic level, demonstrated that some spontaneous tumors of old Wistar rats could contain GH, TSH or ACTH as well as PRL.

The Fine Structure of Irradiated Mouse Heart

1976 ◽  
Vol 34 ◽  
pp. 184-185
Author(s):  
Vivian V. Yang ◽  
S. Phyllis Stearner
Keyword(s):  
Microscopic Level ◽  
Ultrastructural Changes ◽  
Mouse Heart ◽  
Blood Vessel Wall ◽  
Histopathological Changes ◽  
Light Microscopic Level ◽  
Γ Rays ◽  
Fission Neutrons ◽  
Total Body

The heart is generally considered a radioresistant organ, and has received relatively little study after total-body irradiation with doses below the acutely lethal range. Some late damage in the irradiated heart has been described at the light microscopic level. However, since the dimensions of many important structures of the blood vessel wall are submicroscopic, investigators have turned to the electron microscope for adequate visualization of histopathological changes. Our studies are designed to evaluate ultrastructural changes in the mouse heart, particularly in the capillaries and muscle fibers, for 18 months after total-body exposure, and to compare the effects of 240 rad fission neutrons and 788 rad 60Co γ-rays.Three animals from each irradiated group and three control mice were sacrificed by ether inhalation at 4 days, and at 1, 3, 6, 12, and 18 months after irradiation. The thorax was opened and the heart was fixed briefly in situwith Karnofsky's fixative.

Ultrastructure of primary spermatocyte in fish (Tilapia: Oreochromis niloticus): The synaptonemal complex

1986 ◽  
Vol 44 ◽  
pp. 288-289
Author(s):  
T. Guha ◽  
A. Q. Siddiqui ◽  
P. F. Prentis
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Synaptonemal Complex ◽  
Oreochromis Niloticus ◽  
Short Communication ◽  
Primary Spermatocyte ◽  
Mitotic Division ◽  
Meiotic Cell ◽  
Electron Microscope Level ◽  
Homologous Chromosomes

The Primary Spermatocytes represent a stage in spermatogenesis when the first meiotic cell division occurs. They are derived from Spermatogonium or Stem cell through mitotic division. At the zygotene phase of meiotic prophase the Synaptonemal complex appears in these cells in the space between the paired homologous chromosomes. Spermatogenesis and sperm structure in fish have been studied at the electron microscope level in a few species? However, no work has yet been reported on ultrastructure of tilapia, O. niloticus, spermatozoa and spermatogenetic process. In this short communication we are reporting the Ultrastructure of Primary Spermatocytes in tilapia, O. niloticus, and the fine structure of synaptonemal complexes seen in the spermatocyte nuclei.

Sign in / Sign up

Export Citation Format

Share Document