Investigation of metabolites produced by Magnaporthe oryzae during appressorium development using 1H NMR metabolomics approach

This study was aimed to determine metabolites produced by Magnaporthe oryzae and identify metabolic changes during appressorium development. Appressorium development were induced in vitro and subjected to 1H NMR spectroscopy for metabolites production and multivariate data analysis. PCA, PLS-DA and OPLS-DA were used to profile metabolite production throughout appressorium development. There were 43 metabolites identified putatively and PCA showed differences of metabolites production between mycelium and appressorium development. Metabolites that were significantly produced (p < 0.05) during appressorium development including isocitrate, isobutyrate, lysine, glutamate, succinate, tyrosine, choline, glycerol, xylose, mannose, sucrose, tryptophan, butyrate, leucine, isoleucine, valine, ethanol, methylmalonate, threonine, lactate, alanine, arginine, 4-aminobutyrate, homoserine, glucose, mannitol and glucitol. Glycerolipid, carbohydrates and amino acids metabolisms showed to be highly involved during appressoria development. This study revealed metabolites produced by M. oryzae during appressoria development in vitro as first metabolomics data using 1H NMR approach.

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Aromatic amino acids play a harmonizing role in prostate cancer: A metabolomics-based cross-sectional study

International Journal of Reproductive BioMedicine ◽

10.18502/ijrm.v19i8.9622 ◽

2021 ◽

pp. 741-750

Author(s):

Ziba Akbari ◽

Roghayeh Taghipour Dijojin ◽

Zahra Zamani ◽

Reza Haji Hosseini ◽

Mohammad Arjmand

Keyword(s):

Prostate Cancer ◽

Amino Acids ◽

Nmr Spectroscopy ◽

1H Nmr ◽

1H Nmr Spectroscopy ◽

Aromatic Amino Acids ◽

Proton Nuclear Magnetic Resonance ◽

Least Squares Regression ◽

Cross Sectional ◽

Metabolomics Data

Background: Prostate cancer (PCa) is a common health problem worldwide. The rate of this disease is likely to grow by 2021. PCa is a heterogeneous disorder, and various biochemical factors contribute to the development of this disease. The metabolome is the complete set of metabolites in a cell or biological sample and represents the downstream end product of the omics. Hence, to model PCa by computational systems biology, a preliminary metabolomics-based study was used to compare the metabolome profile pattern between healthy and PCa men. Objective: This study was carried out to highlight energy metabolism modification and assist the prognosis and treatment of disease with unique biomarkers. Materials and Methods: In this cross-sectional research, 26 men diagnosed with stage-III PCa and 26 healthy men with normal PSA levels were enrolled. Urine was analyzed with proton nuclear magnetic resonance (1H-NMR) spectroscopy, accompanied by the MetaboAnalyst web-based platform tool for metabolomics data analysis. Partial least squares regression discriminant analysis was applied to clarify the separation between the two groups. Outliers were documented and metabolites determined, followed by identifying biochemical pathways. Results: Our findings reveal that modifications in aromatic amino acid metabolism and some of their metabolites have a high potential for use as urinary PCa biomarkers. Tryptophan metabolism (p < 0.001), tyrosine metabolism (p < 0.001), phenylalanine, tyrosine and tryptophan biosynthesis (p < 0.001), phenylalanine metabolism (p = 0.01), ubiquinone and other terpenoid-quinone biosynthesis (p = 0.19), nitrogen metabolism (p = 0.21), and thiamine metabolism (p = 0.41) with Q2 (0.198) and R2 (0.583) were significantly altered. Conclusion: The discriminated metabolites and their pathways play an essential role in PCa causes and harmony. Key words: Metabolomics, Prostate cancer, Aromatic amino acids, 1H-NMR spectroscopy.

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Discrimination of Enantiomers of Amides with Two Stereogenic Centers Enabled by Chiral Bisthiourea Derivatives Using 1H NMR Spectroscopy

Organic & Biomolecular Chemistry ◽

10.1039/d1ob00742d ◽

2021 ◽

Author(s):

Hanchang Zhang ◽

Hongmei Zhao ◽

Jie Wen ◽

Zhanbin Zhang ◽

Pericles Stavropoulos ◽

...

Keyword(s):

Amino Acids ◽

Nmr Spectroscopy ◽

1H Nmr ◽

1H Nmr Spectroscopy ◽

Chiral Recognition ◽

Stereogenic Centers

Enantiomers of a few new amides containing two stereogenic centers have been derived from D- and L-α-amino acids as guests for chiral recognition by 1H NMR spectroscopy. A variety of...

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Molecular characterization of human healthy and neoplastic cerebral and renal tissues by in vitro 1H NMR spectroscopy (Review)

International Journal of Molecular Medicine ◽

10.3892/ijmm.9.3.299 ◽

2002 ◽

Cited By ~ 1

Author(s):

M. Tosi ◽

G. Fini ◽

A. Tinti ◽

A. Reggiani ◽

V. Tugnoli

Keyword(s):

Nmr Spectroscopy ◽

Molecular Characterization ◽

1H Nmr ◽

1H Nmr Spectroscopy

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In vitro metabolism of [2-13C]-ethanol by 1H NMR spectroscopy using 13C decoupling with the reverse dept polarization-transfer pulse sequence

Biochemical and Biophysical Research Communications ◽

10.1016/0006-291x(85)91651-1 ◽

1985 ◽

Vol 128 (1) ◽

pp. 107-112 ◽

Cited By ~ 7

Author(s):

William M. Brooks ◽

Leith N. Moxon ◽

James Field ◽

Michael G. Irving ◽

David M. Doddrell

Keyword(s):

Nmr Spectroscopy ◽

1H Nmr ◽

1H Nmr Spectroscopy ◽

Pulse Sequence ◽

Polarization Transfer ◽

In Vitro Metabolism

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Effects of amino acids on development in vitro of cleavage-stage bovine embryos into blastocysts

Reproduction Fertility and Development ◽

10.1071/rd9960835 ◽

1996 ◽

Vol 8 (5) ◽

pp. 835 ◽

Cited By ~ 28

Author(s):

T Pinyopummintr ◽

BD Bavister

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Embryo Development ◽

Culture Medium ◽

Essential Amino Acids ◽

Bovine Embryo ◽

Bovine Embryos ◽

Development In Vitro ◽

Defined Culture

Effects of amino acids on early bovine embryo development in vitro were examined using a chemically-defined, protein-free culture medium. Bovine embryos produced in vitro were cultured from 18 h to 72 h post insemination in a simple medium containing lactate as the only energy source except for the amino acid treatments. Subsequently, embryos were transferred to TCM-199 supplemented with serum for blastocyst development to substantiate their developmental competence. Treatments were: (1) non-essential amino acids from TCM-199 (NEA); (2) essential amino acids from TCM-199 (EA); (3) NEA+EA; (4) Eagle's minimum essential medium amino acids (MEM AA); (5) 11 amino acids present in HECM-6 (11 AA); and (6) 0.2 mM glutamine (GLN). A higher proportion of embryos (percentage of inseminated ova) cleaved to the > or = 8-cell stage by 72 h post insemination in NEA (56.7%), EA (41.2%), 11 AA (40.3%) and GLN (51.1%) than in either NEA+EA (30.0%) or MEM AA (33.1%). However, after transfer to complex medium, embryos that had developed in EA, as well as those in MEM AA or NEA+EA, produced significantly fewer blastocysts (37.1%, 34.4% and 25.6% respectively) than those in NEA (56.7%), GLN (48.9%) or 11 AA (37.7%). The ability of blastocysts to hatch from their zonae pellucidae was also affected by amino acid treatment during cleavage stages. The present study indicated that the addition of NEA or GLN or 11 AA to a chemically-defined culture medium during the cleavage phase of bovine embryo development increases their subsequent ability to reach the blastocyst stage. These data have implications for understanding the nutritional needs of bovine embryos produced in vitro and for optimizing the composition of culture media to support their development.

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ChemInform Abstract: pH Dependence of the Stability of Boric Acid and Borate Esters of Amino Acids and Amino Diols in Aqueous Medium as Studied by 11B and 1H NMR Spectroscopy.

ChemInform ◽

10.1002/chin.198937076 ◽

1989 ◽

Vol 20 (37) ◽

Author(s):

J. VAN HAVEREN ◽

J. A. PETERS ◽

J. G. BATELAAN ◽

A. P. G. KIEBOOM ◽

H. VAN BEKKUM

Keyword(s):

Amino Acids ◽

Nmr Spectroscopy ◽

Boric Acid ◽

Aqueous Medium ◽

1H Nmr ◽

1H Nmr Spectroscopy ◽

Ph Dependence ◽

Borate Esters ◽

The Stability

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In vitro Expression, Characterisation and Subcellular Localisation of AvrPi54 Protein, an Effector of Rice Blast Fungus Magnaporthe oryzae

10.20944/preprints201906.0031.v1 ◽

2019 ◽

Author(s):

Banita Kumari Saklani ◽

Kirti Arora ◽

Ravi Kumar Asthana ◽

Tilak Raj Sharma

Keyword(s):

Amino Acids ◽

Host Cell ◽

Magnaporthe Oryzae ◽

Cell Biology ◽

Plant Pathogens ◽

Plant Cells ◽

Onion Bulb ◽

Subcellular Localisation ◽

Pathogenesis Related

Plant pathogens utilize effectors to subvert host cell biology for facilitating infection. However certain effectors, called as the avirulence proteins, trigger immune responses in the host. AvrPi54 is an avirulence protein from fungus Magnaporthe oryzae which induces the defense reactions in rice cells that contain Pi54 resistance gene. The characterization of such proteins and elucidation of their function facilitate the understanding of the mechanism of disease establishment. In present study, physiochemical properties of AvrPi54 were analysed using computational methods. We found that mature AvrPi54 is a small, hydrophobic and secretary protein with 134 amino acids. It is rich in small amino acids with no significant homology with other proteins in databases. The gene ORF was cloned in pET28a(+) vector and expressed in E. coli BL21(DE3)pLysS. The protein was purified using affinity chromatography. Transient expression of the gene in epidermal cells of onion bulb is a powerful tool to predict the subcellular localization in plant cells. We fused AvrPi54 to eYFP and transformed epidermal cell layer of onion bulb. Fused protein localized to the plasma membrane and nucleus of plant cells. Therefore, AvrPi54 might be functioning in the host cell as transcription factor or chromatin remodelling factor to modify pathogenesis-related processes.

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Branched-Chain Amino Acids Can Predict Mortality in ICU Sepsis Patients

Nutrients ◽

10.3390/nu13093106 ◽

2021 ◽

Vol 13 (9) ◽

pp. 3106

Author(s):

Alexander Christian Reisinger ◽

Florian Posch ◽

Gerald Hackl ◽

Gunther Marsche ◽

Harald Sourij ◽

...

Keyword(s):

Amino Acids ◽

Septic Shock ◽

Nmr Spectroscopy ◽

1H Nmr ◽

1H Nmr Spectroscopy ◽

Outcome Prediction ◽

Branched Chain Amino Acids ◽

Proton Nuclear Magnetic Resonance ◽

Branched Chain ◽

And Control

Sepsis biomarkers and potential therapeutic targets are urgently needed. With proton nuclear magnetic resonance (1H NMR) spectroscopy, several metabolites can be assessed simultaneously. Fifty-three adult medical ICU sepsis patients and 25 ICU controls without sepsis were prospectively enrolled. 1H NMR differences between groups and associations with 28-day and ICU mortality were investigated. In multivariate metabolomic analyses, we found separate clustering of ICU controls and sepsis patients, as well as septic shock survivors and non-survivors. Lipoproteins were significantly different between sepsis and control patients. Levels of the branched-chain amino acids (BCAA) valine (median 43.3 [29.0–53.7] vs. 64.3 [47.7–72.3] normalized signal intensity units; p = 0.005), leucine (57.0 [38.4–71.0] vs. 73.0 [54.3–86.3]; p = 0.034) and isoleucine (15.2 [10.9–21.6] vs. 17.9 [16.1–24.4]; p = 0.048) were lower in patients with septic shock compared to those without. Similarly, BCAA were lower in ICU non-survivors compared to survivors, and BCAA were good discriminators for ICU and 28-day mortality. In uni- and multivariable logistic regression analyses, higher BCAA levels were associated with decreased ICU- and 28-day mortality. In conclusion, metabolomics using 1H NMR spectroscopy showed encouraging potential for personalized medicine in sepsis. BCAA was significantly lower in sepsis non-survivors and may be used as early biomarkers for outcome prediction.

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