Molecular characterization of Streptococcus agalactiae group B (SGB) isolated from pregnant women in Rio de Janeiro

Consequence of Streptococcus agalactiae, Group B Streptococcus (GBS) relating infant’s diseases are well documented. Although many women carry this bacterium in their vagina, they may transfer to their infant during delivery and may result in different neonatal invasive diseases. The aim of this study was to determine the prevalence of GBS and serotyping the isolated species among un-selective non-pregnant women who attended two gynecology clinics in Tehran. In this cross-sectional study, a total of 560 vaginal samples collected from non-pregnant women. Following inoculation of the specimen on Blood Agar, the standard technology was applied for the final identification of GBS. Detected GBS species were further confirmed using specific PCR directed on dlts gene. Capsular serotyping was done by using the multiplex PCR method. The chi-square method was used for statistical analysis. Fifty (8.9%) out of 560 non-pregnant women were carriers of GBS. The most common types were III (36%), followed by type II (32%), Ia (26%), and Ib (6%), respectively. Results represent that the prevalence rate of GBS in non-pregnant women was reliable and similar to what obtained from pregnant women. In addition, the serotype III was found the most dominant types, as well as other investigations in the Tehran area. Therefore, vaccine designation based on type III is recommended.

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Molecular characterization of Staphylococcus aureus isolates carrying the Panton-Valentine leukocidin genes from Rio de Janeiro hospitals

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2015.09.004 ◽

2015 ◽

Vol 83 (4) ◽

pp. 331-334 ◽

Cited By ~ 7

Author(s):

Raiane Cardoso Chamon ◽

Natalia Lopes Pontes Iorio ◽

Sthefanie da Silva Ribeiro ◽

Fernanda Sampaio Cavalcante ◽

Kátia Regina Netto dos Santos

Keyword(s):

Staphylococcus Aureus ◽

Molecular Characterization ◽

Rio De Janeiro ◽

Panton Valentine Leukocidin ◽

Valentine Leukocidin

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ESBL Mediated Antimicrobial Nonsusceptibility of Uropathogenic Escherichia coli and Klebsiella pneumoniae Isolates from Pregnant Women in Nnewi, Nigeria

Journal of Advances in Microbiology ◽

10.9734/jamb/2019/v18i230166 ◽

2019 ◽

pp. 1-13

Author(s):

O. Peculiar-Onyekere, Chioma ◽

C. Agbo, Martina ◽

A. Eze, Emmanuel

Keyword(s):

Pregnant Women ◽

Molecular Characterization ◽

Uropathogenic Escherichia Coli ◽

Public Health Issue ◽

Anambra State ◽

E Coli ◽

Positive Isolate ◽

Synergy Test ◽

Plasmid Profiling

Background and Objective: Extended Spectrum β-lactamase (ESBL) producing Urinary Tract Infection (UTI) is an important public health issue due to lack of therapeutic antibiotic options and the danger it portends to the pregnant woman. This study was carried out to determine the prevalence and response to antimicrobials of ESBL-producing uropathogenic E. coli and K. pneumoniae, among pregnant women on ante natal care. Study Design/Materials and Methods: Two hundred and fifteen pregnant women across three different hospitals in Nnewi North L.G.A of Anambra State were screened for these uropathogens. Modified Double Disc Synergy test (MDDST) was carried out on the isolates to phenotypically determine the presence of ESBL. Plasmid profiling as well as plasmid curing studies were undertaken. Molecular characterization of the phenotypically confirmed ESBL positive isolate via Polymearse Chain Reaction (PCR) was carried out using three ESBL primers (bla-TEM, bla-SHV, bla-CTX-M). Results: 192 isolates were obtained of which 75(39.1%) were E. coli and 117(60.9%) were K. pneumoniae. A total of 130 (67.7%) of the pregnant women had ESBL-mediated UTI, the highest rate reported in recent times in Nigeria. Molecular characterization of the ESBL types revealed a predominance of bla-TEM (91.9%), followed by bla-SHV (73.3%) and bla-CTX-M (56.8%). Conclusion: The Majority of the isolates harbored multiple ESBL genes. Curing studies were largely ineffectual as most of the isolates retained their resistance determinants regardless of the concentration of the curing agent (acridine orange).

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