Molecular Characterization of Streptococcus agalactiae Isolates From Pregnant and Non-Pregnant Women at Yazd University Hospital, Iran

Background and Objective: Extended Spectrum β-lactamase (ESBL) producing Urinary Tract Infection (UTI) is an important public health issue due to lack of therapeutic antibiotic options and the danger it portends to the pregnant woman. This study was carried out to determine the prevalence and response to antimicrobials of ESBL-producing uropathogenic E. coli and K. pneumoniae, among pregnant women on ante natal care. Study Design/Materials and Methods: Two hundred and fifteen pregnant women across three different hospitals in Nnewi North L.G.A of Anambra State were screened for these uropathogens. Modified Double Disc Synergy test (MDDST) was carried out on the isolates to phenotypically determine the presence of ESBL. Plasmid profiling as well as plasmid curing studies were undertaken. Molecular characterization of the phenotypically confirmed ESBL positive isolate via Polymearse Chain Reaction (PCR) was carried out using three ESBL primers (bla-TEM, bla-SHV, bla-CTX-M). Results: 192 isolates were obtained of which 75(39.1%) were E. coli and 117(60.9%) were K. pneumoniae. A total of 130 (67.7%) of the pregnant women had ESBL-mediated UTI, the highest rate reported in recent times in Nigeria. Molecular characterization of the ESBL types revealed a predominance of bla-TEM (91.9%), followed by bla-SHV (73.3%) and bla-CTX-M (56.8%). Conclusion: The Majority of the isolates harbored multiple ESBL genes. Curing studies were largely ineffectual as most of the isolates retained their resistance determinants regardless of the concentration of the curing agent (acridine orange).

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COLONIZATION PREVALENCE AND SUSCEPTIBILITY OF Streptococcus agalactiae IN PREGNANT WOMEN AT HUSM

Saúde (Santa Maria) ◽

10.5902/223658342391 ◽

2011 ◽

Vol 36 (1) ◽

pp. 9 ◽

Cited By ~ 1

Author(s):

Adriane Regina Veit ◽

Magda Cristina Souza Marques Roehrs ◽

Leticia Eichstaedt Mayer ◽

Silvana Oliveira Santos ◽

Rosieli Martini ◽

...

Keyword(s):

Pregnant Women ◽

Streptococcus Agalactiae ◽

Early Onset ◽

Serological Test ◽

Neonatal Infection ◽

University Hospital ◽

Sensitivity Tests ◽

Maternal Fetal Transmission ◽

Intrapartum Antibiotic ◽

Santa Maria

ABSTRACTThe Streptococcus agalactiae colonization prevalence and its susceptibility to antimicrobials in pregnant women at University Hospital of Santa Maria (HUSM) were evaluated from June to December 2009. The vaginal-rectal material was inoculated into tubes containing Todd-Hewitt broth with subsequent subculture on blood agar. The GBS identification was made through presumptive tests, confirmed by serological test and its susceptibility was evaluated. The occurrence of GBS maternal-fetal transmission in the colonized pregnant women was researched. The GBS colonization was 11.11%._All strains were susceptible to penicillin, ampicillin, and vancomycin. Two strains (50%) were intermediate to clindamycin and one (25%) intermediate to erythromycin._A newborn whose mother was colonized had early-onset neonatal infection by GBS. By this, it is very important the research about the colonization by GBS in all pregnant women from 35 to 37 weeks of gestation and the use of intrapartum antibiotic prophylaxis for colonized pregnant women.Descriptors: Streptococcus agalactiae; Prevalence; Pregnant women; Newborns; Microbial Sensitivity Tests. RESUMOAvaliou-se a prevalência de colonização pelo Streptococcus agalactiae e o seu perfil de sensibilidade frente aos antimicrobianos em gestantes no Hospital Universitário de Santa Maria (HUSM), de junho a dezembro de 2009. O material vaginal-retal foi inoculado em tubos contendo caldo Todd-Hewitt com posterior subcultura em ágar sangue. A identificação do EGB foi realizada através de testes presuntivos, confirmadas por teste sorológico e avaliado seu perfil de sensibilidade. Pesquisou-se ocorrência de transmissão materno-fetal do EGB nas gestantes colonizadas. A prevalência de colonização foi de 11,11%. Todas as cepas foram sensíveis à penicilina, ampicilina e vancomicina. Duas cepas (50%) foram intermediárias à clindamicina e uma (25%) intermediária à eritromicina. Um recém-nascido de mãe colonizada teve infecção neonatal de início precoce por EGB. Confirma-se a importância da pesquisa de colonização por EGB em todas as gestantes entre 35ª e 37ª semana de gestação e uso de quimioprofilaxia intraparto nas gestantes colonizadas.Descritores: Streptococcus agalactiae; Prevalência; Gestantes; Recém-nascido; Testes de Sensibilidade Microbiana.

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Prevalence and antimicrobial susceptibility profile of Streptococcus agalactiae in pregnant women seen at the University Hospital of Londrina, Paraná, Brazil

Semina Ciências Biológicas e da Saúde ◽

10.5433/1679-0367.2018v39n1p ◽

2018 ◽

Vol 39 (1) ◽

pp. 77

Author(s):

Ana Elisa Belotto Morguette ◽

Renata Perugini Biasi-Garbin ◽

Eliane Saori Otaguiri ◽

Marcia Regina Eches Perugini ◽

Marsileni Pelisson ◽

...

Keyword(s):

Pregnant Women ◽

Antimicrobial Susceptibility ◽

Streptococcus Agalactiae ◽

University Hospital ◽

Antimicrobial Susceptibility Profile ◽

Para Determinar ◽

The University

Um estudo retrospectivo foi realizado com gestantes atendidas no Hospital Universitário de Londrina, Paraná, Brasil para determinar a prevalência de colonização vaginal-retal por Estreptococos do Grupo B (EGB) e o perfil de sensibilidade de EGB aos antimicrobianos utilizados para a antibioticoterapia profilática intraparto. Swabs vaginais-retais foram coletados de 2.901 mulheres entre a 35ª e 37ª semana de gestação. Destes, 528 (18,2%) apresentaram cultura positiva para EGB, e 0,4%, 10,2% e 10% dos isolados foram resistentes à penicilina, eritromicina e clindamicina, respectivamente. Estes resultados destacam a importância de vigilância contínua da colonização por EGB em gestantes para a prevenção de infecções em neonatos por EGB.

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