scholarly journals INDUKSI KALUS CENGKEH DARI EKSPAN DAUN MENGGUNAKAN 2,4-D SECARA IN VITRO

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Yulianti Rasud ◽  
Zainuddin Basri ◽  
Nirwan Sahiri
Keyword(s):  
Growth Regulators ◽  
Callus Induction ◽  
Callus Formation ◽  
Cell Mass ◽  
Medium Composition ◽  
Ms Medium ◽  
Plant Parts ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRACT Callus induction is one method of tissue culture which is done by stimulating cell division continuously from certain plant parts such as leaves, roots, stems, and so on by using growth regulators to form cell mass. The cell mass (callus) will then regenerate through organogenesis or embryogenesis to become a new plant. One of the growth regulators used for callus induction is 2,4-D. The aims of this experiments was to evaluate the best concentration of 2,4-D for callus induction of clove leaves. The experiment used Completely Randomized Design with treatment tested was concentrations of 2,4-D, consisted of six levels, namely 0.5 ppm, 1.0 ppm, 1.5 ppm, 2.0 ppm, 2.5 ppm and 3.0 ppm. Results of this experiments indicated that the best medium composition for callus induction was MS medium supplemented with 0.5 ppm 2,4-D.  In the medium composition, the fastest callus formation, namely 6.00 weeks after culture and the percentage of callus formation reached 100% with the color and texture of the resulting callus white and crumb. Keyword : Callus Induction, Clove, 2,4-DABSTRAK Induksi kalus merupakan salah satu metode kultur jaringan yang dilakukan dengan jalan memacu pembelahan sel secara terus menerus dari bagian tanaman tertentu seperti daun, akar, batang, dan sebagainya dengan menggunakan zat pengatur tumbuh hingga terbentuk massa sel. Massa sel (kalus) tersebut selanjutnya akan beregenerasi melalui organogenesis ataupun embriogenesis hingga menjadi tanaman baru. Salah satu zat pengatur tumbuh yang digunakan untuk induksi kalus adalah 2,4-D. Penelitian ini bertujuan menentukan konsentrasi 2,4-D yang lebih baik untuk induksi kalus daun cengkeh.  Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan tiga kali ulangan. Media dasar yang digunakan adalah media MS yang ditambahkan berbagai konsentrasi 2,4-D yaitu 0,50 ppm, 1,5 ppm, 2 ppm, 2,5 ppm, dan 3 ppm. Hasil penelitian menunjukkan bahwa komposisi media yang terbaik untuk induksi kalus daun cengkeh adalah media MS yang ditambahkan 0,5 ppm 2,4-D.  Pada komposisi media tersebut diperoleh saat muncul kalus paling cepat, yaitu rata-rata 6,00 MST dengan persentase pembentukan kalus tertinggi mencapai 100% dengan warna dan tekstur kalus yang dihasilkan putih dan remah. Kata Kunci :  Induksi Kalus, Cengkeh, 2,4-D.

scholarly journals PERTUMBUHAN KALUS DAUN MELON (Cucumis melo) VARIETAS MAI 119 DENGAN PEMBERIAN BAP (BENZYL AMINO PURINE) DAN 2,4-D (2,4 DICHLOROPHENOXYACETIC ACID)

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Nining Intan Toharah ◽  
Dwi Soelistya Dyah Jekti ◽  
Lalu Zulkifli
Keyword(s):  
Growth Regulators ◽  
Callus Induction ◽  
Cucumis Melo ◽  
Callus Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Benzyl Amino Purine

This study aims to determine the concentration of growth regulators BAP and   2,4-D which have the highest effect in stimulating the formation of callus melon plants (Cucumis melo) Mai 119 variety. Completely randomized design (CRD) was used in this research. Media used on callus induction was MS medium with addition of several concentration of BAP  (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) and 2,4-D (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) either alone or in a combination of both. Parameters measured were the time appearing of callus, callus diameter, callus texture, and callus color. Anova followed by Tukey's test was used to the analyse of time appearing of callus. Data of callus diameter was analyzed using Kruskal Wallis test followed by Mann-Whitney test. In the analysis of parameter related to the callus texture and callus color, descriptive test were used. The results showed that there were differences in the effect of growth regulators on the callus formation. The fastest callus induction and the largest diameter of callus were obtained on media with concentration of 2 mg/L BAP and 3 mg/L BAP.Keywords: BAP (benzyl amino purine), 2,4-D (2,4-dichlorophenoxyacetic acid), callus induction, melon (Cucumis melo) varieties Mai 119

scholarly journals PENGGUNAAN 2,4-D UNTUK INDUKSI KALUS KLON KAKAO UNGGUL SULAWESI 1

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Yulianti Rasud ◽  
Moh. Habil ◽  
Tony Tony
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Culture Techniques ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Difference Test ◽  
Honestly Significant Difference Test

ABSTRACT The multiplication of cocoa clones in conventional Sulawesi has not yet been able to fulfill the demand for large quantities of seeds because it is limited by the number of shoots and branches ready to be tapped, connected and oculated and takes longer to produce large quantities of seeds. One alternative in overcoming this problem is plant proragation using tussue culture techniques.  The aim of this experiment was to determine the appropriate of 2,4-D for callus induction of superior cocoa clones Sulawesi via in vitro culture.  This experiment used Completely Randomized Design with five treatments, namely 0.50 ppm 2,4-D, 0.75 ppm 2,4-D, 1.00 ppm 2,4-D, 1.25 ppm 2,4-D and 1.50 ppm 2,4-D.  Parameters observed consisted of the time, percentage, color and texture of calli.  Data was analized by using analysis of variance and differences between mean treatments were determined by Honestly Significant Difference Test at 5% level.  Results of this experiment indicated that the ability of different callus induction at various concentrations of 2,4-D for superior cocoa clones in Sulawesi 1 was tried.  it was obtained the quickest callus formation at concentration 0.50ppm 2,4-D namely average 4.22 WAC with the percentage of callus formation was up to 99,33%. Keywords: Callus Induction, Clones Sulawesi 1, 2,4-D ABSTRAK Perbanyakan klon kakao Sulawesi secara konvensional saat ini belum dapat memenuhi permintaan bibit dalam jumlah besar karena sangat dibatasi oleh jumlah tunas dan cabang yang siap disetek, disambung, dan diokulasi serta dibutuhkan waktu yang lebih lama untuk menghasilkan bibit dalam jumlah besar. Salah satu alternatif dalam mengatasi masalah tersebut adalah perbanyakan tanaman dengan menggunakan teknik kultur jaringan.  Penelitian ini bertujuan untuk memperoleh protokol yang tepat dalam menginduksi kalus sebagai upaya awal dalam perbanyakan tanaman kakao melalui embryogenesis. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 5 level perlakuan yaitu 0,50 ppm 2,4-D, 0,75 ppm 2,4-D, 1,00 ppm 2,4-D, 1,25 ppm 2,4-D dan 1,50 ppm 2,4-D. Pengamatan dilakukan terhadap saat muncul kalus, persentase eksplan berkalus, warna kalus dan tekstur kalus.  Data diolah dengan analisis ragam dan perbedaan antar perlakuan ditentukan dengan Uji Beda Nyata Jujur pada taraf 5%. Hasil penelitian menunjukkan kemampuan induksi kalus berbeda pada berbagai konsentrasi 2,4-D untuk klon kakao unggul Sulawesi 1 yang dicobakan. Saat muncul kalus paling cepat diperoleh pada konsentrasi 0,5 ppm 2,4-D yaitu rata-rata 16,67 HST dengan persentase pembentukan kalus tertinggi mencapai 99,33%.  Selanjutnya, warna dan tekstur kalus yang dihasilkan yaitu remah putih dan remah kecoklatan. Kata Kunci: Induksi Kalus, Klon Sulawesi 1, 2,4-D.

scholarly journals Callus Induction on True Shallot Seed Explant Using a Combination of BA and 2,4-D

2018 ◽  
Vol 2 (3) ◽  
pp. 137 ◽  
Author(s):  
Krisna Dharmayanti ◽  
Endang Sulistyaningsih ◽  
Rani Agustina Wulandari
Keyword(s):  
Callus Induction ◽  
In Vitro Selection ◽  
Callus Formation ◽  
Optimum Combination ◽  
Shoot Height ◽  
Randomized Design ◽  
Allium Cepa L ◽  
Callus Proliferation ◽  
Completely Randomized Design

BA and 2,4-D combination were commonly used for in vitro culture of Shallot (Allium cepa L. var agregatum 2n = 2x = 16) to induce callus, but there was no information for callus induction on shallot seed (TSS) explant. Callus could be utilized for in vitro selection and generating of genetic variation. The aims of the research was to identify the response of TSS (Trisula and Tuk Tuk) as explant  and to obtain the optimum combination of BA and 2,4-D (mg.L-1): (0–0, 2–1, 2–2, 2–3, and 2–4) in callus induction. The research had been carried out in the Tissue Culture Laboratory, Faculty of Agriculture, Universitas Gadjah Mada during the year 2015-2016. Factorial treatments of variety and growth regulators were arranged in Completely Randomized Design with four replications. Data of percentage of germination, shoot height, root length, percentage of callus formation, callus weight, and chromosomes number of callus were recorded. The results showed that combination of 2 mg.L-1 BA + (1– 4) mg.L-1 2,4-D induced callus formation on TSS but inhibits shoots and roots growth. The best callus proliferation was at a concentration of 1 mg.L-1 2,4-D. Tetraploid callus chromosomes (2n = 4x = 32) was detected in Trisula grown in the 2 mg.L-1 BA + 4 mg.L-1 2,4-D, but in the Tuk Tuk callus did not detected the changing of chromosomes number.

scholarly journals Induksi Kalus Tanaman Kentang Dombu (Solanum tuberosum L.) Secara In Vitro Dengan Pemberian ZPT 2,4-D (Dichlorophenoxy Acetid Acid)

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Siti RLR Idris ◽  
Asri Pirade Paserang
Keyword(s):  
Solanum Tuberosum ◽  
Callus Induction ◽  
Culture Media ◽  
Callus Formation ◽  
Solanum Tuberosum L ◽  
Randomized Design ◽  
Completely Randomized Design

This research was aimed to determine the effect of the PGR 2.4-D (Dichlorophenoxy Acetid Acid) in various concentrations on induceing callus of Dombu potato (Solanum tuberosum L.). This research was performed based on Completely Randomized Design (RAL) with 6 treatments and 3 times repetation, so there were 18 experiment units. Each experiment used 3 explants so as there were 54 explants. The combination of concentration of the tested PGR in the culture media were T1 = MS0+ 2.4-D 0 ppm (control), T2 = MS0+ 2.4-D 0.5 ppm, T3 = MS0+ 2.4-D 1 ppm, T4= MS0+ 2.4-D 1.5 ppm, T5 = MS0+ 2.4-D 2 ppm and T6 = MS0+ 2.4-D 2.5 ppm. The results showed that callus induction was appeared in the concentration of 1.0, 1.5, 2.0, and 2.5 ppm. Callus was generally formed on the Day-8 after plantation. Callus color was mostly greenish transparent, callus texture was mostly crumb type, and callus formation percentage was almost 100%. The best media for inducing the callus was in treatment T4 (1.5 ppm 2.4-D), it referred to the formed callus biomass

scholarly journals Callus Derived Regeneration of Some Selected Brassica Genotypes

2018 ◽  
Vol 15 (2) ◽  
pp. 1-10
Author(s):  
S K Biswas ◽  
M Z Tareq ◽  
S Ahmmed ◽  
A B M Z Hoque ◽  
M T Rahman
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Brassica Species ◽  
Direct Organogenesis ◽  
Stem Explants ◽  
Root Induction ◽  
Ms Medium ◽  
Cotyledon Explants ◽  
Randomized Design ◽  
Completely Randomized Design

The experiment was conducted to observe the callus induction ability of Brassica species. Plantlets were regenerated from cotyledon and stem explants of Brassica napus, Brassica campestris and Brassica juncea through direct organogenesis. The experiments were conducted in a Completely Randomized Design (CRD) with 4 replications. The highest frequency of callus formation was recorded in MS containing 2.0 mgl-1 BAP, 0.5 mgl-1 NAA and 2.0 mgl-1 AgNO3 in both stem and cotyledon explants. Among these explants, stem was found to be better responsive in callus induction than cotyledon. Among the genotypes used, BINA Sarisha-4 induced the highest percentage (100.00%) of callus from stem explants which was followed by BINA Sarisha-5 (100.00%) and Sampad (83.35%). On the other hand, BINA Sarisha-4 induced callus from 91.67% cotyledon explants, followed by BINA Sarisha-5 (75.00%) and Sampad (66.67%). Similarly, the highest percentage of shoot regeneration (58.34%) from stem explants of BINA Sarisha-4 was observed in MS medium supplemented with combination of hormone and silver nitrate concentrations. The highest percentage of root induction was 66.67 and 58.33% in plantlets derived from stem and cotyledon explants, respectively in ½ MS medium supplemented with 2.0 mgl-1 IBA and 0.5 mgl-1 of NAA. The highest survival rate was found after acclimatization of plants derived from stem (77.78%) and cotyledon (64.28%) explants of BINA Sarisha-4 in pot and 64.33 and 55.55%, respectively in field.The Agriculturists 2017; 15(2) 01-10

scholarly journals GERMINAÇÃO E PROPAGAÇÃO IN VITRO DE MOGNO BRASILEIRO (Swietenia macrophylla King)

Nativa ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 595-599
Author(s):  
Carolina Dias Pereira ◽  
Cristiani Santos Bernini ◽  
Márcia Regina Jantsch ◽  
Reginaldo Antonio Medeiros ◽  
Luciana Coelho de Moura
Keyword(s):  
Native Species ◽  
Callus Formation ◽  
Economic Value ◽  
Selective Logging ◽  
Ms Medium ◽  
Swietenia Macrophylla ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Para Determinar

A intensificação da exploração seletiva de madeiras tem ocasionado grandes perdas na biodiversidade de espécies nativas de alto valor econômico, comprometendo, a sua sobrevivência. O potencial madeireiro do mogno brasileiro é mundialmente reconhecido e, por isso, é também motivo de grande preocupação da comunidade científico. Esta pesquisa objetiva avaliar o efeito de concentrações de reguladores de crescimento na germinação e multiplicação in vitro de mogno brasileiro e analisar aspectos físicos para determinar a eficiência na produção de mudas. Para isso, as sementes foram incubadas em meio de cultura MS no delineamento inteiramente casualisado em esquema fatorial 2 x 4 (duas intensidades de luz e quatro tempos de hipoclorito de sódio), com cinco repetições e quatro sementes por repetição. Aos trinta dias, os explantes isentos de contaminação foram transferidos para tubos de ensaio contendo meio MS e suplementados com diferentes concentrações de BAP e mantidos em sala de crescimento. Para multiplicação os brotos foram transferidos para meio MS e suplementados com diferentes concentrações de BAP e ANA. Obtiveram-se a maior porcentagem de brotações (83%) de explantes da porção intermediária de mogno e a utilização de concentrações superiores de ANA e BAP para formação de calos permitindo êxito na produção clonal. Palavras-chave: espécie nativa; plantas lenhosas; micropropagação.   Germination and propagation in vitro of brazilian mahogany (Swietenia macrophylla King)   ABSTRACT: The intensification of selective logging causes great losses biodiversity of native species of high economic value, compromising their survival. The wood industry potential of brazilian mahogany is recognized worldwide and, therefore, it is also a cause of great preoccupations of the scientific community. This research aims to evaluate the effect of concentrations of growth regulators on germination and in vitro multiplication of brazilian mahogany and analyze physical aspects to determining the efficiency in the production of seedlings. For this, the seeds were incubated in MS culture medium in a completely randomized design in a factorial scheme 2 x 4 (two light intensities and four sodium hypochlorite times) with five repetitions and four seeds per supplemented with different concentrations of BAP and kept in the repetition. At thirty days, explants free from contamination were transferred to test tubes containing MS medium and supplemented with different BAP concentrations and kept in the growth room. For multiplication the shoots were transferred to MS medium and supplemented with different concentrations of BAP and ANA. The highest number of percentage of shoots (83%) in the use of explants of the intermediate mahogany and the higher concentrations of ANA and BAP for callus formation enabling success in clonal production. Keywords: native species; woody plants; micropropagation.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

scholarly journals Callus Induction in Baru (Dipteryx alata Vog.) Explants

2018 ◽  
Vol 47 (2) ◽  
pp. 538-543
Author(s):  
Rodrigo Kelson S. REZENDE ◽  
Ana Maria N. SCOTON ◽  
Maílson V. JESUS ◽  
Zeva V. PEREIRA ◽  
Fernanda PINTO
Keyword(s):  
Ascorbic Acid ◽  
Callus Induction ◽  
Callus Formation ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Alternative Technique ◽  
Propagation Methods ◽  
Dipteryx Alata

Baru (Dipteryx alata Vog.) is a species with great economic and environmental potential; it has popular acceptance, besides being a very productive species. Alternative propagation methods are important for species maintenance and exploration. Thus, micropropagation emerged as an alternative technique, providing genetic stability and the production of a large number of seedlings. The aim of the present investigation was to develop a callus induction protocol for in vitro baru explants. The tested explants were nodal, internodal and foliar segments. The explants were disinfected for 30 seconds in 70% alcohol (v/v) and 2 minutes in sodium hypochlorite (1.25% active chlorine). This was followed by triple washing. The inoculation was carried out in test tubes containing 15 mL MS medium (30 g L-1 sucrose, 6 g L-1 agar and 100 mg L-1 ascorbic acid) supplemented with 2.0 mg L-1 naphthalene acetic acid (NAA). The solution also contained 0.0, 2.5 or 5.0 mg L-1 of 6-benzylaminopurine (BAP) with the pH adjusted to 5.8. In the incubation phase, the explants were cultured for seven days in the dark and then subjected to a photoperiod of 16 hours (43 µmol m-2 s-1) at 25 ± 2 °C. The treatments were studied with 2.5, 5.0, 7.5 or 10.0 mg L-1 BAP additions to the MS. Callus formation, contamination and oxidation evaluations were undertaken. The results obtained when using 2.0 mg L-1 NAA concluded that such a treatment should be used to induce callogenesis from nodal explants, while for the tested baru leaf explants, the best results for callus formation were given by the combination of 2.0 mg L-1 NAA with 2.5 mg L-1 of BAP to.

scholarly journals In vitro callus induction of gerbera from leaf explant

2020 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Sadia Afrin Jui ◽  
Md. Mijanur Rahman Rajib ◽  
M. Mofazzal Hossain ◽  
Sharmila Rani Mallik ◽  
Iffat Jahan Nur ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Leaf Explants ◽  
Leaf Explant ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Indole 3 Acetic Acid

The experiment was designed to evaluate the effect of growth regulators on leaf explant of Gerbera for callus induction. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Indole-3-acetic acid (IAA) were used to initiate cultures. These were added to Murashige and Skoog medium in different combinations and concentrations. Leaf explants cultured on MS medium supplemented with BAP+ 2, 4-D+ IAA in T4 treatment & BAP+ 2,4-D in T5 treatment showed the best results for callus induction. On the other hand callus was induced early in the combination of BA+ 2,4-D + IAA hormone in T5, T9 & T8 treatment respectively. The rate of callus induction was very low in BA + NAA combinations but it was much earlier.   

scholarly journals Haploid plantlet regeneration through anther culture in oilseed Brassica species

1970 ◽  
Vol 34 (4) ◽  
pp. 693-703 ◽  
Author(s):  
MA Alam ◽  
MA Haque ◽  
MR Hossain ◽  
SC Sarker ◽  
R Afroz
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Growth Regulators ◽  
Callus Induction ◽  
Brassica Species ◽  
Plantlet Regeneration ◽  
Root Induction ◽  
Ms Medium ◽  
Half Strength

Anther of five varieties of Brassica species, namely BARI Shariaha-7, Tori-7, Agrani, Daulat and Safal were cultured in vitro to observe their regeneration potentiality. Different concentrations and combinations of growth regulators were supplemented in MS medium. The range of callus induction was 12.50-87.50 %. Maximum callus induction (75.00%) was observed on MS +4 mg/L 2, 4-D + 1.0 mg/L BAP. Among the genotypes, BARI Sharisha-7 showed the highest percentage of callus induction (60.42%). Among the treatments, highest percentage of shoot regeneration (75.00%) was observed on MS + 4 mg/L BAP + 1.0 mg/L NAA. BARI Sharisha-7 also showed the highest rate of plant regeneration (66.67%). Root induction was highest (75%) on half strength MS medium supplemented with 1.0 mg/L IBA and 0.5 mg/L NAA. The plantlets with sufficient roots thus obtained were transferred successfully to plastic pots and subsequently to the field. BARI Sharisha-7 and Tori-7 survived easily in the pots as well as in the field but Safal was very poor in survivability both in the pots and in the field. Key Words: Brassica; haploid; anther culture; in vitro regeneration.DOI: 10.3329/bjar.v34i4.5844Bangladesh J. Agril. Res. 34(4) : 693-703, December 2009 

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