scholarly journals ACTIVITY OF MITOCHONDRIAL ANTIOXIDANT DEFENSE SYSTEM IN YOUNG PATIENTS WITH GASTROESOPHAGEAL REFLUX DISEASE

2020 ◽  
Vol 7 (4) ◽  
pp. 164-167
Author(s):  
Olga Kovalyova ◽  
Tamara Pasiieshvili
Keyword(s):  
Superoxide Dismutase ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Reflux Disease ◽  
Manganese Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Young Patients ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Manganese Superoxide

Background. Despite numerous studies, the pathogenesis of gastroesophageal reflux disease remains unclear. Aim of research: assessment the activity of antioxidant defense system in young patients with GERD based on expression of biomarker associated with mitochondrial function. Material and methods. The study included 45 patients with gastroesophageal reflux disease. The examined contingent was presented by students age from 18 to 25 years. 20 healthy persons were included as control group. Levels of manganese superoxide dismutase were determined in blood serum of study persons with enzyme immunoassays (ELISA, Elabscience, USA). Statistical data processing by the Statistica Basic Academic 13 for Windows En local was made. Results. Gastroesophageal reflux disease in young patients is characterized by significantly increasing of manganese superoxide dismutase as compare to control group (7.1700 ng/ml vs 4.4720 ng/ml respectively, p<0.01). Presence of erosion in esophagus mucous doesn't accompanied by significant changes of evaluated parameter as compare with non-erosion form of disease in patients. Conclusion. The elevation in young patients with GERD the biomarker of mitohondrial antioxidant defense system we may speculate as adaptive response contributing to non-specific citoprotection. Taking to account the publishing facts about dual role of manganese superoxide dismutase it is necessary to monitoring antioxidant enzyme in patients with gastroesophageal reflux disease for prediction of possible complications and outcome.

scholarly journals Cryptococcus neoformans Mitochondrial Superoxide Dismutase: an Essential Link between Antioxidant Function and High-Temperature Growth

2005 ◽  
Vol 4 (1) ◽  
pp. 46-54 ◽  
Author(s):  
Steven S. Giles ◽  
Ines Batinić-Haberle ◽  
John R. Perfect ◽  
Gary M. Cox
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Cryptococcus Neoformans ◽  
Manganese Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Elevated Temperatures ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Poor Growth ◽  
Manganese Superoxide

ABSTRACT Manganese superoxide dismutase is an essential component of the mitochondrial antioxidant defense system of most eukaryotes. In the present study, we used a reverse-genetics approach to assess the contribution of the Cryptococcus neoformans manganese superoxide dismutase (Sod2) for antioxidant defense. Strains with mutations in the SOD2 gene exhibited increased susceptibility to oxidative stress as well as poor growth at elevated temperatures compared to isogenic wild-type strains. The sod2Δ mutants were also avirulent in a murine model of inhaled cryptococcosis. Reconstitution of a sod2Δ mutant restored Sod2 activity, eliminated the oxidative stress and temperature-sensitive (ts) phenotypes, and complemented the virulence phenotype. Characterization of the ts phenotype revealed a dependency between Sod2 antioxidant activity and the ability of C. neoformans cells to adapt to growth at elevated temperatures. The ts phenotype could be suppressed by the addition of either ascorbic acid (10 mM) or Mn salen (200 μM) at 30°C, but not at 37°C. Furthermore, sod2Δ mutant cells that were incubated for 24 h at 37°C under anaerobic, but not aerobic, conditions were viable when shifted to the permissive conditions of 25°C in the presence of air. These data suggest that the C. neoformans Sod2 is a major component of the antioxidant defense system in this human fungal pathogen and that adaptation to growth at elevated temperatures is also dependent on Sod2 activity.

scholarly journals Enzymative And Non-Enzymative Components of the Antioxidant Defense System in Young Patients with Gastroesophageal Reflux Disease and Autoimmune Thyroiditis

2021 ◽  
Vol 6 (1) ◽  
pp. 107-112
Author(s):  
T. M. Pasiieshvili ◽  
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Antioxidant System ◽  
Autoimmune Thyroiditis ◽  
Reflux Disease ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Total Antioxidant Activity ◽  
Defense System ◽  
Total Antioxidant

Antioxidant defense is considered in the context of a universal system, which, together with the components of the immune system, provides hemodynamic balance and antigenic homeostasis of the body, that is, the work of this system, represented by two practically independent links. The first line of the antioxidant system, the work of which is associated with the activity of superoxide dismutase, catalase, ceruloplasmin and a number of other enzymatic and non-enzymatic components, is considered an initial barrier, provides support at a constant controlled level of free radical oxidation. However, there is insufficient data in previous studies regarding the antioxidant defense system in patients with gastrointestinal disorders associated with thyroid disease. The purpose of the work was to establish the state of the antioxidant system in terms of total antioxidant activity and 8-isoprostane in young people with gastroesophageal reflux disease and autoimmune thyroiditis. Material and methods. The work involved 120 people with a combined course of gastroesophageal reflux disease and autoimmune thyroiditis at the age from 18 to 25 years. All patients had the same social status (students), which made it possible to equalize both physical and emotional stress, peak and stressful situations. Results and discussion. We examined the indicators of total antioxidant activity: the aggregate value of enzymes of the first level of the antioxidant system and 8-isoprostane, the level of the non-enzymatic link in antioxidant protection. These indicators depended on the morphological form of damage to the mucous membrane of the esophagus: they were more expressive in the erosive form of esophagitis. Thus, the combined course of gastroesophageal reflux disease and autoimmune thyroiditis is accompanied by changes in the indicators of the antioxidant system as a result of an active inflammatory process in the esophageal mucosa. There is a decrease in the total antioxidant activity, the first line of the antioxidant system, the value of which depends on morphological changes in the esophageal mucosa. Conclusion. An active inflammatory process in patients with gastroesophageal reflux disease and autoimmune thyroiditis leads to an increase in 8-isoprostane, an indicator of a non-enzymatic link in the antioxidant defense system

The role of antioxidant system dysfunction in the course of gastroesophageal reflux disease in young patients with autoimmune thyroiditis

2021 ◽  
Author(s):  
T. M. Pasiieshvili
Keyword(s):  
Antioxidant Activity ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Antioxidant Defense ◽  
Reduced Glutathione ◽  
Young Patients ◽  
Antioxidant Defense System ◽  
Total Antioxidant Activity ◽  
Defense System ◽  
Total Antioxidant

Objective — to determine the state of the antioxidant defense system by studying the total antioxidant activity and indicators of the glutathione system in young patients with gastroesophageal reflux disease (GERD) and autoimmune thyroiditis (AIT). Materials and methods. The study involved 120 patients with GERD and AIT (mean age — 21.9 ± 2.7 уears) and 45 subjects with isolated GERD (mean age — 21.2 ± 2.4 уears). All the investigated patients were the students of higher educational institutions. The general antioxidant activity was investigated as the aggregate indicator of the first phase of antioxidant defense and the state of the glutathione link of the antioxidant system (the activity of glutathione reductase, glutathione peroxidase and reduced glutathione). Results. It has been established that in patients with isolated GERD the total antioxidant activity of blood serum was significantly lower in comparison with the control group and was significantly suppressed with the addition of AIT. The decreased synthesis of glutathione reductase, glutathione peroxidase and reduced glutathione was reveled at the comorbid pathologies, as well as dependence of these parameters from the morphological forms of the esophageal mucous lesions: the most intensive alterations were observed  at the erosive form of the disease. Conclusions. The combination of GERD and AIT was accompanied by the changes in the antioxidant defense system parameters. This was manifested in the inhibition of the indicator of the total antioxidant activity and the enzymatic component of the second phase of the system — the glutathione link. These changes were dependent on the morphological form of the esophageal lesions.  

scholarly journals TOTAL ANTIOXIDANT ACTIVITY AND MANGANESE SUPEROXIDE DISMUTASE IN COMORBIDITY OF GASTROESOPHAGEAL REFLUX DISEASE AND AUTOIMMUNE THYROIDITIS IN STUDENT POPULATION

2020 ◽  
Vol 73 (12) ◽  
pp. 2644-2650
Author(s):  
Tamara M. Pasiieshvili ◽  
Natalia M. Zhelezniakova ◽  
Lyudmila M. Pasiyeshvili ◽  
Olga M. Kovalyova
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Autoimmune Thyroiditis ◽  
Reflux Disease ◽  
Manganese Superoxide Dismutase ◽  
Total Antioxidant Activity ◽  
Manganese Superoxide ◽  
Total Antioxidant

The aim: To determine the state of total antioxidant activity and the content of the mitochondrial enzyme – manganese superoxide dismutase in students with gastroesophageal reflux disease (GERD) and autoimmune thyroiditis (AIT) comorbidity. Materials and methods: 120 patients with GERD and AIT, 45 patients with GERD and 42 patients with AIT were examined. The patients involved in the study were students. The international standardized GerdQ questionnaire was used. Total antioxidant activity and manganese superoxide dismutase levels were determined. Results: The study showed that comorbidity of GERD and AIT was accompanied with a significant increase in the frequency and intensity of heartburn, sleep disorders and the use of additional medications to relieve symptoms. Patients with GERD and AIT had significantly lower total antioxidant activity than patients with isolated GERD and isolated AIT. At the same time, the manganese superoxide dismutase level was significantly higher in group with comorbidity than in patients with isolated diseases. Conclusions: The presence of concomitant AIT in patients with GERD among the student population contributes to some deviations in the clinical characteristics, inhibition of total antioxidant activity and activation of manganese superoxide dismutase.

scholarly journals Antioxidant system in implementation of endotoxicosis syndrome in young persons with comorbidity of gastroesophageal reflux disease and autoimmune thyroiditis

2020 ◽  
Keyword(s):  
Superoxide Dismutase ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Antioxidant System ◽  
Autoimmune Thyroiditis ◽  
Reflux Disease ◽  
Manganese Superoxide Dismutase ◽  
Total Antioxidant Status ◽  
Second Phase ◽  
Manganese Superoxide

Introduction. In recent years, there has been an increase in the prevalence of gastroesophageal reflux disease and autoimmune thyroiditis in young people. This is associated with a hereditary predisposition, viral diseases, which are often recorded in children's and adolescent groups, the unreasonable use of a large number of drugs, as well as the influence of various negative environmental factors, instability of the autonomic nervous system, eating disorders, the presence of bad habits, chronic stress and others. The aim of the work was to investigate the state of total antioxidant status and the content of biomarkers of the second phase of detoxification of xenobiotics - manganese superoxide dismutase and glutathione peroxidase in young people with gastroesophageal reflux disease and autoimmune thyroiditis. Materials and methods. To achieve this goal, 120 patients with a combined course of gastroesophageal reflux disease (GERD) and autoimmune thyroiditis (AIT) were examined, which made up the main group and two comparison groups: with isolated GERD (45 people) and AIT (42 patients). All patients were students of various universities and were between the ages of 18 and 25. Duration of the disease did not exceed 3 years. The total antioxidant status as an indicator of the first phase of defense and the levels of mitochondrial manganese superoxide dismutase and glutathione peroxidase – the second phase of antioxidant defense was determined. Results. The study showed that in all surveyed groups there was a decrease of total antioxidant status, which characterizes the first phase of the antioxidant defense system. Changes in the first phase of antioxidant defense affect the indicators of the second level of the system, which became the basis for studying the activity of mitochondrial manganese superoxide dismutase. Thus, the value of this indicator in all examined individuals exceeded the norm, which confirmed the inclusion of the second controlling phase of antioxidant system into the pathological process. At the same time, an increase inhibition of the glutathione defense link, the second phase of xenobiotic detoxification, were diagnosed. Conclusions. Negative changes occur in the enzyme system of the first line of antioxidant system in patients with GERD and AIT which may be the result of an unfavorable autoimmune background. Control over the work of antioxidant system moves to the second phase of the system, manifested by an increase in the content of mitochondrial manganese superoxide dismutase and inhibition of the activity of glutathione peroxidase in patients with a combination of GERD and AIT.

scholarly journals Increased Activity of Hippocampal Antioxidant Enzymes as an Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats

2017 ◽  
Vol 67 (4) ◽  
pp. 540-550 ◽  
Author(s):  
Nataša Popović ◽  
B. Snežana Pajović ◽  
Vesna Stojiljković ◽  
Ana Todorović ◽  
Snežana Pejić ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Manganese Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Copper Zinc Superoxide Dismutase ◽  
Protein Levels ◽  
Copper Zinc ◽  
Increased Activity

AbstractThis study examined the effects of chronic restraint stress (CRS: 2 hours × 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activities of SOD 1, SOD 2 and CAT in the hippocampus of chronically stressed rats. Investigated parameters were quantifi ed by using real-time RT-PCR, Western blot analysis and assay of enzymatic activity. We found that CRS did not change mRNA and protein levels of SOD 1 and CAT, but increased mRNA and protein levels of SOD 2. However, CRS treatment increased the enzyme activities of SOD 1, SOD 2 and CAT. Our fi ndings indicate that the increased activity of antioxidant enzymes (SOD 1, SOD 2 and CAT) in the hippocampus may be an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats.

scholarly journals Effects of increased concentrations of chloride on the expression of Mn-SOD enzyme in tobacco

2017 ◽  
Vol 109 (1) ◽  
pp. 53
Author(s):  
Akbar Norastehnia ◽  
Parvaneh SHEYDAEI
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Manganese Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Antioxidant Response ◽  
Defense System ◽  
Reverse Transcription Pcr ◽  
Manganese Superoxide ◽  
System Activation

<p>Chlorine is one of the ions contributing to salinity, despite being an essential micronutrient. Cl<sup>-</sup> absorption takes place more easily than other nutrients so, the toxic effects of chlorine on the growth has considered rather than its scarcity. Salt stress can ultimately leads to oxidative stress through ROS increase and antioxidant defense system is induced. Therefore, in this study the effect of different concentration of chlorine in irrigation water on the expression of manganese superoxide dismutase was investigated as an indicator of antioxidant defense system activation. Seedlings of tobacco were treated with different concentrations, i.e. 2, 4, 8 mM of CaCl<sub>2</sub>. Evaluation of Mn-SOD isoenzyme gene expression was performed using RT-qPCR (quantitative reverse transcription PCR) at 0, 3, 6 and 12 hours after treatment. The results showed Mn-SOD gene transcription increased after 3 h treatment with 8 mM CaCl<sub>2</sub> and peaked at 6 hours. Based on the observed changes, concentrations of calcium chloride greater than 8 mM in water used for irrigation of tobacco causes stress that results in activation of antioxidant response.</p>

Effects of morphological changes in the esophageal mucosa in gastroesophageal reflux disease on the indicators of the antioxidant system in students with autoimmune thyroiditis

2021 ◽  
Author(s):  
T. M. Pasiieshvili
Keyword(s):  
Antioxidant Activity ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Glutathione Peroxidase ◽  
Reflux Disease ◽  
Manganese Superoxide Dismutase ◽  
Esophageal Mucosa ◽  
Total Antioxidant Activity ◽  
Manganese Superoxide ◽  
Total Antioxidant

Objective — to establish changes in indicators of the antioxidant defence system, taking into account the morphological form of the esophageal mucosa lesions in patients with gastroesophageal reflux disease (GERD), occurring against the background of autoimmune thyroiditis (AIT). Materials and methods. The study involved 120 students with GERD and AIT, aged 18 to 25 years; from them 93 (77.5 %) females and 27 (22.5 %) males. The disease duration did nit exceed 3 years. Fibrogastroduodenoscopic examination has shown an erosive form of gastroesophageal reflux disease in 34 patients (28.3 %) and non‑erosive form of the disease was in 86 cases (71.7 %). Indicators of the total antioxidant activity of glutathione peroxidase and mitochondrial manganese superoxide dismutase were examined. Results. A decrease in the total indicator of the total antioxidant activity was established, which was estimated as the failure of the first phase of the antioxidant defence system. At the same time, an increase in the activity of manganese superoxide dismutase and a decrease in the production of glutathione peroxidase were noted. The levels of total antioxidant activity and glutathione peroxidase correlated with morphological changes in the esophageal mucosa: the presence of the erosive form of the disease had a greater impact on the changes in these parameters. Conclusions. The combined course of gastroesophageal reflux disease and autoimmune thyroiditis in students was accompanied by a decrease in the indicators of total antioxidant activity and glutathione peroxidase and an increase in the activity of manganese superoxide dismutase. The presence of erosions in the esophageal mucosa had a greater impact on the changes in the parameters of total antioxidant activity and glutathione peroxidase.  

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