The role of antioxidant system dysfunction in the course of gastroesophageal reflux disease in young patients with autoimmune thyroiditis

2021 ◽  
Author(s):  
T. M. Pasiieshvili
Keyword(s):  
Antioxidant Activity ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Antioxidant Defense ◽  
Reduced Glutathione ◽  
Young Patients ◽  
Antioxidant Defense System ◽  
Total Antioxidant Activity ◽  
Defense System ◽  
Total Antioxidant

Objective — to determine the state of the antioxidant defense system by studying the total antioxidant activity and indicators of the glutathione system in young patients with gastroesophageal reflux disease (GERD) and autoimmune thyroiditis (AIT). Materials and methods. The study involved 120 patients with GERD and AIT (mean age — 21.9 ± 2.7 уears) and 45 subjects with isolated GERD (mean age — 21.2 ± 2.4 уears). All the investigated patients were the students of higher educational institutions. The general antioxidant activity was investigated as the aggregate indicator of the first phase of antioxidant defense and the state of the glutathione link of the antioxidant system (the activity of glutathione reductase, glutathione peroxidase and reduced glutathione). Results. It has been established that in patients with isolated GERD the total antioxidant activity of blood serum was significantly lower in comparison with the control group and was significantly suppressed with the addition of AIT. The decreased synthesis of glutathione reductase, glutathione peroxidase and reduced glutathione was reveled at the comorbid pathologies, as well as dependence of these parameters from the morphological forms of the esophageal mucous lesions: the most intensive alterations were observed  at the erosive form of the disease. Conclusions. The combination of GERD and AIT was accompanied by the changes in the antioxidant defense system parameters. This was manifested in the inhibition of the indicator of the total antioxidant activity and the enzymatic component of the second phase of the system — the glutathione link. These changes were dependent on the morphological form of the esophageal lesions.  

scholarly journals Enzymative And Non-Enzymative Components of the Antioxidant Defense System in Young Patients with Gastroesophageal Reflux Disease and Autoimmune Thyroiditis

2021 ◽  
Vol 6 (1) ◽  
pp. 107-112
Author(s):  
T. M. Pasiieshvili ◽  
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Antioxidant System ◽  
Autoimmune Thyroiditis ◽  
Reflux Disease ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Total Antioxidant Activity ◽  
Defense System ◽  
Total Antioxidant

Antioxidant defense is considered in the context of a universal system, which, together with the components of the immune system, provides hemodynamic balance and antigenic homeostasis of the body, that is, the work of this system, represented by two practically independent links. The first line of the antioxidant system, the work of which is associated with the activity of superoxide dismutase, catalase, ceruloplasmin and a number of other enzymatic and non-enzymatic components, is considered an initial barrier, provides support at a constant controlled level of free radical oxidation. However, there is insufficient data in previous studies regarding the antioxidant defense system in patients with gastrointestinal disorders associated with thyroid disease. The purpose of the work was to establish the state of the antioxidant system in terms of total antioxidant activity and 8-isoprostane in young people with gastroesophageal reflux disease and autoimmune thyroiditis. Material and methods. The work involved 120 people with a combined course of gastroesophageal reflux disease and autoimmune thyroiditis at the age from 18 to 25 years. All patients had the same social status (students), which made it possible to equalize both physical and emotional stress, peak and stressful situations. Results and discussion. We examined the indicators of total antioxidant activity: the aggregate value of enzymes of the first level of the antioxidant system and 8-isoprostane, the level of the non-enzymatic link in antioxidant protection. These indicators depended on the morphological form of damage to the mucous membrane of the esophagus: they were more expressive in the erosive form of esophagitis. Thus, the combined course of gastroesophageal reflux disease and autoimmune thyroiditis is accompanied by changes in the indicators of the antioxidant system as a result of an active inflammatory process in the esophageal mucosa. There is a decrease in the total antioxidant activity, the first line of the antioxidant system, the value of which depends on morphological changes in the esophageal mucosa. Conclusion. An active inflammatory process in patients with gastroesophageal reflux disease and autoimmune thyroiditis leads to an increase in 8-isoprostane, an indicator of a non-enzymatic link in the antioxidant defense system

scholarly journals ACTIVITY OF MITOCHONDRIAL ANTIOXIDANT DEFENSE SYSTEM IN YOUNG PATIENTS WITH GASTROESOPHAGEAL REFLUX DISEASE

2020 ◽  
Vol 7 (4) ◽  
pp. 164-167
Author(s):  
Olga Kovalyova ◽  
Tamara Pasiieshvili
Keyword(s):  
Superoxide Dismutase ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Reflux Disease ◽  
Manganese Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Young Patients ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Manganese Superoxide

Background. Despite numerous studies, the pathogenesis of gastroesophageal reflux disease remains unclear. Aim of research: assessment the activity of antioxidant defense system in young patients with GERD based on expression of biomarker associated with mitochondrial function. Material and methods. The study included 45 patients with gastroesophageal reflux disease. The examined contingent was presented by students age from 18 to 25 years. 20 healthy persons were included as control group. Levels of manganese superoxide dismutase were determined in blood serum of study persons with enzyme immunoassays (ELISA, Elabscience, USA). Statistical data processing by the Statistica Basic Academic 13 for Windows En local was made. Results. Gastroesophageal reflux disease in young patients is characterized by significantly increasing of manganese superoxide dismutase as compare to control group (7.1700 ng/ml vs 4.4720 ng/ml respectively, p<0.01). Presence of erosion in esophagus mucous doesn't accompanied by significant changes of evaluated parameter as compare with non-erosion form of disease in patients. Conclusion. The elevation in young patients with GERD the biomarker of mitohondrial antioxidant defense system we may speculate as adaptive response contributing to non-specific citoprotection. Taking to account the publishing facts about dual role of manganese superoxide dismutase it is necessary to monitoring antioxidant enzyme in patients with gastroesophageal reflux disease for prediction of possible complications and outcome.

scholarly journals Parameters of Lipid Peroxidation and Antioxidant Protection in Men with Pathological Spermogram Variants

2019 ◽  
Vol 4 (1) ◽  
pp. 14-18
Author(s):  
Nadezhda A. Kurashova ◽  
Ekaterina A. Kudeyarova ◽  
Ekaterina O. Kuznetsova
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Total Antioxidant Activity ◽  
Antioxidant Protection ◽  
Defense System ◽  
Total Antioxidant ◽  
Peroxidation Processes

Background.Today infertile marriage is not only a serious medical, but also a socio-demographic and economic problem. Male factor contributes averagely to half of the cases of the disease in couples. Such factors as high levels of reactive oxygen species and oxidative stress have been reported to compromise the process of spermatogenesis and sperm function in men. Oxidative stress is a significant risk factor for male infertility. A pro-oxidant testicular environment may alter the expression profile of functional sperm proteins and result in poor sperm quality.Aims.To study the characteristics of the intensity of the processes of lipoperoxidation and antioxidant status in the ejaculate of men with different variants of spermograms.Materials and methods.We examined 69 men with primary infertility and 155 fertile men. The content of lipid peroxidation components and antioxidant protection was determined by spectrophotometric method.Results.The results of the study in men with infertility and asthenozoospermia showed decreased total antioxidant activity of sperm by 50 % and α-tocopherol by 52 %, and in men with infertility and oligozoospermia, decreased total antioxidant activity of sperm by 47 % and α-tocopherol by 41 %.Conclusions.The analysis indicates a change in the parameters of the system of lipid peroxidation – antioxidant defense system and confirms the development of oxidative stress in them. Depending on the pathological state of the ejaculate in men of reproductive age, lipid peroxidation processes have their own characteristics. In men with oligozoospermia, peroxidation processes occur more intensively. Activation of lipid peroxidation – antioxidant defense system processes can be both a consequence and a cause of various metabolic changes in the human body.

scholarly journals TOTAL ANTIOXIDANT ACTIVITY AND INFLAMMATION MARKERS DURING PROGRESSION OF GASTROESOPHAGEAL REFLUX DISEASE IN YOUNG PATIENTS WITH AUTOIMMUNE THYROIDITIS

2021 ◽  
Vol 21 (1) ◽  
pp. 44-48
Author(s):  
T.M. Pasiieshvili
Keyword(s):  
Antioxidant Activity ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Autoimmune Thyroiditis ◽  
Reflux Disease ◽  
Young Patients ◽  
Total Antioxidant Activity ◽  
Histological Changes ◽  
Oesophageal Mucosa ◽  
Total Antioxidant

The aim of this study is to establish the content of inflammation markers (tumour necrosis factor alpha and interleukin 18) and the index of total antioxidant activity in young patients with comorbidity between gastroesophageal reflux disease and autoimmune thyroiditis and to determine their dependence on morpho-histological changes in the oesophageal mucosa. Materials and methods. The study involved 182 students aged 18 to 25 years: 120 patients with comorbidity between gastroesophageal reflux disease and autoimmune thyroiditis, 42 those having an isolated course of autoimmune thyroiditis. The methodology included complex clinical, instrumental, immunological, statistical methods. Results. Determining the activity of proinflammatory cytokines has revealed their statistically significant increase. The level of these cytokines depends on morpho-histological changes in the mucous membrane and reaches the maximum during the erosive process. At the same time, the indicator of total antioxidant activity significantly decreases. In this case, the erosive form of the disease is accompanied by more expressive changes in this indicator. Conclusions. The inflammatory process in the oesophagus is accompanied by the activation of the pro-inflammatory segment of immunity against the background of the suppression of the antioxidant system, which is dependent on morphological changes in the oesophageal mucosa. These changes will contribute to the development of pathological apoptosis and the progression of nosologies.

Effects of morphological changes in the esophageal mucosa in gastroesophageal reflux disease on the indicators of the antioxidant system in students with autoimmune thyroiditis

2021 ◽  
Author(s):  
T. M. Pasiieshvili
Keyword(s):  
Antioxidant Activity ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Glutathione Peroxidase ◽  
Reflux Disease ◽  
Manganese Superoxide Dismutase ◽  
Esophageal Mucosa ◽  
Total Antioxidant Activity ◽  
Manganese Superoxide ◽  
Total Antioxidant

Objective — to establish changes in indicators of the antioxidant defence system, taking into account the morphological form of the esophageal mucosa lesions in patients with gastroesophageal reflux disease (GERD), occurring against the background of autoimmune thyroiditis (AIT). Materials and methods. The study involved 120 students with GERD and AIT, aged 18 to 25 years; from them 93 (77.5 %) females and 27 (22.5 %) males. The disease duration did nit exceed 3 years. Fibrogastroduodenoscopic examination has shown an erosive form of gastroesophageal reflux disease in 34 patients (28.3 %) and non‑erosive form of the disease was in 86 cases (71.7 %). Indicators of the total antioxidant activity of glutathione peroxidase and mitochondrial manganese superoxide dismutase were examined. Results. A decrease in the total indicator of the total antioxidant activity was established, which was estimated as the failure of the first phase of the antioxidant defence system. At the same time, an increase in the activity of manganese superoxide dismutase and a decrease in the production of glutathione peroxidase were noted. The levels of total antioxidant activity and glutathione peroxidase correlated with morphological changes in the esophageal mucosa: the presence of the erosive form of the disease had a greater impact on the changes in these parameters. Conclusions. The combined course of gastroesophageal reflux disease and autoimmune thyroiditis in students was accompanied by a decrease in the indicators of total antioxidant activity and glutathione peroxidase and an increase in the activity of manganese superoxide dismutase. The presence of erosions in the esophageal mucosa had a greater impact on the changes in the parameters of total antioxidant activity and glutathione peroxidase.  

scholarly journals TOTAL ANTIOXIDANT ACTIVITY AND MANGANESE SUPEROXIDE DISMUTASE IN COMORBIDITY OF GASTROESOPHAGEAL REFLUX DISEASE AND AUTOIMMUNE THYROIDITIS IN STUDENT POPULATION

2020 ◽  
Vol 73 (12) ◽  
pp. 2644-2650
Author(s):  
Tamara M. Pasiieshvili ◽  
Natalia M. Zhelezniakova ◽  
Lyudmila M. Pasiyeshvili ◽  
Olga M. Kovalyova
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Autoimmune Thyroiditis ◽  
Reflux Disease ◽  
Manganese Superoxide Dismutase ◽  
Total Antioxidant Activity ◽  
Manganese Superoxide ◽  
Total Antioxidant

The aim: To determine the state of total antioxidant activity and the content of the mitochondrial enzyme – manganese superoxide dismutase in students with gastroesophageal reflux disease (GERD) and autoimmune thyroiditis (AIT) comorbidity. Materials and methods: 120 patients with GERD and AIT, 45 patients with GERD and 42 patients with AIT were examined. The patients involved in the study were students. The international standardized GerdQ questionnaire was used. Total antioxidant activity and manganese superoxide dismutase levels were determined. Results: The study showed that comorbidity of GERD and AIT was accompanied with a significant increase in the frequency and intensity of heartburn, sleep disorders and the use of additional medications to relieve symptoms. Patients with GERD and AIT had significantly lower total antioxidant activity than patients with isolated GERD and isolated AIT. At the same time, the manganese superoxide dismutase level was significantly higher in group with comorbidity than in patients with isolated diseases. Conclusions: The presence of concomitant AIT in patients with GERD among the student population contributes to some deviations in the clinical characteristics, inhibition of total antioxidant activity and activation of manganese superoxide dismutase.

scholarly journals Antioxidant Potential, Phytochemicals Composition, and Metal Contents of Datura alba

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Waliullah Khan ◽  
Sidra Subhan ◽  
Dilawar Farhan Shams ◽  
Sahib Gul Afridi ◽  
Riaz Ullah ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Defense ◽  
Radical Scavenging ◽  
Pharmaceutical Formulations ◽  
Antioxidant Defense System ◽  
Antioxidant Potential ◽  
Defense System ◽  
Metal Contents ◽  
Stem Extract ◽  
Free Radical Scavenging Assay

This study investigated the phytochemical characteristics and antioxidant activity in leaves, roots, stem, flower, and seed parts of Datura alba (D. alba). The study also assessed the heavy metal (Cr, Mn, Zn, and Cu) accumulation in each part of the plant. Among the phytochemicals, alkaloids were found only in leaves while tannins, flavonoids, and phenols were present in all parts of the plant. For antioxidant activity, free radical scavenging assay for 2,2-diphenyl-1-picrylhydrazyl (DPPH) was performed using ascorbic acid as the standard. Higher activity was shown by stem extract in methanol and leaf extract in n-hexane, ethyl acetate, and chloroform. Furthermore, all the target heavy metals were detected in all plant sections with the highest concentration of Zn in leaves and Cu in stem, root, flower, and seed. Due to stronger antioxidant potential and phytochemical composition, D. alba could prove as valuable prospect in pharmaceutical formulations by taking part in the antioxidant defense system against generation of free radicals.

Salivary Antioxidant and Oxidative Stress Marker Levels in HIV-Positive Individuals

2019 ◽  
Vol 22 (1) ◽  
pp. 59-64
Author(s):  
Samaneh Vaziri Amjad ◽  
Poorandokht Davoodi ◽  
Mohammad Taghi Goodarzi ◽  
Hamidreza Abdolsamadi ◽  
Jalal Poorolajal ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Oxidative Stress Marker ◽  
Hiv Positive ◽  
Control Group ◽  
Defense System ◽  
Positive Group ◽  
Total Antioxidant ◽  
And Oxidative Stress

Background:HIV infections are a worldwide health problem. HIV infection reduces CD4+ cell counts. Oxidative stress might play an important role in the stimulation of virus replication and immunodeficiency. Saliva might be the first line of defense against oxidative stress.Objective:The aim of this study was to evaluate the oxidative stress marker and antioxidant levels of saliva in HIV-infected patients by measuring total antioxidant capacity and malondialdehyde level.Methods:A total of 49 HIV-positive patients and 49 healthy HIV-negative individuals were randomly selected. All the patients were clinically examined. Five mL of unstimulated whole saliva was collected and evaluated by spectrophotometric assay. Data were analyzed with STATA 11.Results:Mean ages of the case and control groups were 28 and 33 years, respectively. Salivary malondialdehyde levels were significantly higher in the HIV-positive group (3.68±2.26) compared to the healthy control group (2.79±1.91). Levels of salivary total antioxidant capacity were significantly lower in the HIV-positive group (0.20± 0.09) compared to the control group (0.27±0.10).Conclusion:The antioxidant defense system in HIV-positive individuals was low and oxidative stress was high in this population. Saliva might be used as a diagnostic tool for antioxidant changes in HIV-positive patients in the future. There were changes in salivary antioxidant defense system and oxidative stress in HIV-positive individuals. Antioxidant supplements might help local salivary and general health statuses.

scholarly journals The antioxidant defense system of Varroa destructor mites facilitates the infestation of Apis mellifera

2016 ◽  
Vol 60 (1) ◽  
pp. 163-166
Author(s):  
Kamila Dmochowska-Ślęzak ◽  
Małgorzata Dmitryjuk ◽  
Ewa Zaobidna ◽  
Krystyna Żółtowska
Keyword(s):  
Antioxidant Enzymes ◽  
Varroa Destructor ◽  
Antioxidant Status ◽  
Antioxidant Defense ◽  
Total Antioxidant Status ◽  
Antioxidant Defense System ◽  
Low Molecular Weight ◽  
Defense System ◽  
Total Antioxidant ◽  
Parasitic Mite

Abstract Varroa destructor is a parasitic mite of the Western honey bee. The activity of five antioxidant enzymes of V. destructor were analysed. Glutathione content and total antioxidant status was also evaluated. Our results suggest that antioxidant enzymes constitute the main line of defense against ROS in V. destructor, whereas low-molecular-weight antioxidants play a limited role in the antioxidant system of mites.

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