scholarly journals Pengaruh terapi oksigen hiperbarik terhadap penyembuhan luka pada luka bakar derajat dua dalam pada hewan coba kelinci

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Rudy H. Susilo ◽  
Mendy Hatibie ◽  
Jan T. Ngantung ◽  
Meilany F. Durry
Keyword(s):  
Wound Healing ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Wound Healing Process ◽  
Burn Wounds ◽  
Degree Burn ◽  
Second Degree

Abstract: Wound healing process consists of inflammation, proliferation, and remodelling phases with increasing inflammatory cells, angiogenesis, and epithelization. Mechanism of hyperbaric oxygen therapy is O2 pressure over 1 ATA will increase oxygen pressure in the tissue. The main outcome measure is wound healing. This study was aimed to obtain the influence of hyperbaric oxygen therapy to wound healing process of deep second degree burn wounds. This was an experimental study. Subject were 36 rabbits divided into 2 groups, each of 18 rabbits. Deep second degree burn wounds were performed on all rabbits. One group was treated with hyperbaric oxygen therapy 2.4 ATA for 6 days, meanwhile the other group as control. The result of Mann-Whitney U test showed significant differences in inflammatory cells (P = 0.025) and epithelization (P = 0.024); albeit, there was not significant difference in angiogenesis (P = 0.442) between the two groups. Conclusion: Hyperbaric oxygen therapy could influence the inflammatory cells and epithelization but not the angiogenesis.Keywords: second degree burn wound, healing process, hyperbaric oxygen therapyAbstrak: Proses penyembuhan luka terdiri dari: fase inflamasi, proliferasi, dan perupaan kembali/remodeling, yang tampak dengan meningkatnya sel-sel radang, angiogenesis serta epitelialisasi. Mekanisme kerja terapi oksigen hiperbarik (TOHB) ialah pemberian tekanan O2 yang melebihi 1 ATA akan menyebabkan peningkatan tekanan O2 dalam jaringan. Jenis penelitian ialah eksperimental. Subyek penelitian 36 ekor kelinci yang dibuat luka bakar derajat dua dalam, kemudian dibagi menjadi dua kelompok, masing-masing 18 ekor. Kelompok perlakuan diberikan TOHB dengan dosis 2,4 ATA selama 6 hari sedangkan kelompok lain sebagai kontrol. Hasil uji Mann-Whitney U menunjukkan terdapat perbedaan bermakna pada jumlah sel radang (P = 0,025) dan epitelialisasi (P = 0,024), tetapi tidak terdapat perbedaan bermakna pada angiogenesis (p=0,442), serta ada perbedaan bermakna pada pada kedua kelompok. Simpulan: Terapi oksigen hiperbarik berpengaruh terhadap jumlah sel radang dan epitelialisasi namun tidak terhadap angiogenesis.Kata kunci: penyembuhan luka bakar, oksigen hiperbarik, luka bakar derajat dua dalam

scholarly journals Fostering a faster post-operative wound healing process with hyperbaric oxygen therapy in a rare case of squamous odontogenic tumor

2021 ◽  
pp. 106718
Author(s):  
Mendy Hatibie Oley ◽  
Maximillian Christian Oley ◽  
Meilany Feronika Durry ◽  
Rizky Natanael Adam ◽  
Deborah Florencia Gunawan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Rare Case ◽  
Oxygen Therapy ◽  
Healing Process ◽  
Odontogenic Tumor ◽  
Wound Healing Process ◽  
Operative Wound

scholarly journals Effects of topical treatment of second-degree burn wounds with Lactobacillus acidophilus on the wound healing process in male rats

2017 ◽  
Vol 3 (3) ◽  
pp. 23-30 ◽  
Author(s):  
Amir Abbas Barzegari ◽  
Masood Hashemzaei ◽  
Raheleh Majdani ◽  
Ali-Reza Alihemmati ◽  
◽  
...  
Keyword(s):  
Wound Healing ◽  
Lactobacillus Acidophilus ◽  
Topical Treatment ◽  
Healing Process ◽  
Male Rats ◽  
Wound Healing Process ◽  
Burn Wounds ◽  
Degree Burn ◽  
Second Degree

673 Effect of Subcutaneous Topical Ozone Therapy on Second Degree Burn Wounds in Rats: An Experimental Study

2021 ◽  
Vol 42 (Supplement_1) ◽  
pp. S193-S193
Author(s):  
Emre Karakaya ◽  
Aydincan Akdur ◽  
H Ebru ◽  
Ayvazoglu Soy ◽  
Alev Ok Atilgan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Male Rats ◽  
Control Group ◽  
Ozone Therapy ◽  
Burn Wounds ◽  
Degree Burn ◽  
Significant Difference ◽  
Second Degree

Abstract Introduction Burn is one of the most severe traumas that causes coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burn may affect the patient’s survival and reduce the complications that may be seen. In the present study we aimed effects of subcutaneous ozone injection on second degree burn wound. Methods A total of 72 Sprague-Dawley male rats included in the study were divided randomly into three groups (control group (CG), silver sulfadiazine group (SG), ozone group (OG)) and each group was divided randomly two subgroups (as sacrificed on d7 and on d14).A deep second degree scald burns were created on the lower back. In CG subcutaneous 0.9% serum saline was injected daily into the burn area. In SG, burns were dressed with silver sulfadiazine daily and in OG subcutaneous ozone was injected daily into the burn area. Tissue hydroxyproline level measurement and histopathological evaluation were done. Results When the groups were compared in terms of weight change, no significant difference was found on the 7th and 14th days. In the evaluation made in terms of tissue hydroxyproline, tissue hydroxyproline level in OG was found to be significantly higher on both the 7th and 14th days (p < 0.001). In histopathological evaluations, it was determined that wound healing in OG was significantly higher than in the other groups. Conclusions According to the results, subcutaneous ozone therapy is more effective than silver sulphadiazine in the healing process of second-degree burn wounds and it can be safely used in the treatment of burn wounds.

scholarly journals Hyperbaric Oxygen Therapy for Second-Degree Burn Healing

2019 ◽  
Vol 32 (3) ◽  
pp. 1-4 ◽  
Author(s):  
Mendy J. Hatibie ◽  
Andi A. Islam ◽  
Mochammad Hatta ◽  
Yefta Moenadjat ◽  
Rudy H. Susilo ◽  
...  
Keyword(s):  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Degree Burn ◽  
Second Degree

scholarly journals Burn Ointment Promoted Cutaneous Wound for Burns and Scalds by Modulating the PI3K/AKT/mTOR Signaling Pathway

2020 ◽  
Author(s):  
Da li Gan ◽  
Yan Yao ◽  
Qi yuan Su ◽  
Su qin Yang ◽  
Li Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Skin Irritation ◽  
Mtor Pathway ◽  
Epithelial Tissue ◽  
Burn Wounds ◽  
Degree Burn ◽  
Tnf Α ◽  
Tgf Β1 ◽  
Second Degree

Abstract Background: Burn injury is common, burn ointment (BO) is a common preparation used to treat burns and scalds in folk as an effective remedy for burn healing. The present study was undertaken to evaluate the healing effect and related underlying mechanisms of BO in a model of deep second-degree thermal burn by animal experiments. Methods: BO is was made up of a combination of extracts from several traditional Chinese medicine and borneol, solid paraffin, rapeseed oil, and and its quality control was assessed. The acute toxicity test and skin irritation test were evaluated by rats. The anti-inflammatory effect was revealed by using inflammation animal models, including the xylene-induced auricle swelling in mice and carrageenan-induced toe swelling in rats. The hot plate test was used to evaluate its analgesic activity. Moreover, the experiments of knife and a deep second-degree burn wound were used to explore the effect of BO in promoting wound healing. On days 7, 14 and 28, the wounds were digitally photographed by a camera and after sacrifice of the SD rats, skin samples were obtained for performing H&E staining, immunohistochemical staining and Western Blotting examination. In addition, The expressed of TNF-α, TGF-β1 and VEGF in serum were detected by ELISA kits. Results: BO had no toxicity or side effects on the skin and liver or kidney function. BO could significantly inhibit auricular swelling in mice, paw welling in rats and markedly prolonged the latencies of licking paws in mice; it also could accelerate the process of wound healing and repair scar by promoting the formation of new epithelial tissue. In addition, BO significantly reduced the content of TNF-α and markedly increased the content of VEGF and TGF-β1 in the serum. Moreover, BO promoted the expression of collagen I. Furthermore, it increased the ratio of p-PI3K/PI3K, p-AKT/AKT and p-mTOR/mTOR in the PI3K / AKT / mTOR pathway.Conclusions: BO could effectively reduce inflammation and pain, and effectively accelerate the healing process of deep second-degree burn wounds. And the mechanism of BO promoting wound healing may be related to activate PI3K / AKT / mTOR pathway. therefore, it may be recommended as a promising topical medication for treating burn wounds in the future clinical trials.

Effect of Subcutaneous Topical Ozone Therapy on Second-Degree Burn Wounds in Rats: An Experimental Study

2021 ◽  
Author(s):  
Emre Karakaya ◽  
Aydincan Akdur ◽  
Ebru Ayvazoğlu Soy ◽  
Coşkun Araz ◽  
Alev Ok Atilgan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Male Rats ◽  
Control Group ◽  
Ozone Therapy ◽  
Burn Wounds ◽  
Degree Burn ◽  
Significant Difference ◽  
Second Degree

Abstract Burns are one of the most severe traumas, causing coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burns may affect rates of patient survival and reduce complications. We studied the effects of subcutaneous ozone injection on second-degree burn wounds in animal model. For this study, 72 Sprague-Dawley male rats were divided randomly into the following three groups: control group , silver sulfadiazine group, and ozone group; each group was then divided randomly into two subgroups (day 7 or day 14 examination and sacrifice). Superficial partial thickness burns were created on the lower back. In the control group, subcutaneous 0.9% serum saline was injected daily into the burn area. In the silver sulfadiazine group, burns were dressed daily with silver sulfadiazine. In the ozone group, subcutaneous ozone was injected daily into the burn area. We performed tissue hydroxyproline level measurements and histopathological evaluations. When groups were compared in terms of weight change, no significant difference was found between day 7 and day 14. With regard to tissue hydroxyproline levels the ozone group had significantly higher levels on both day 7 and day 14 (P < .001). In histopathological evaluations, we determined that wound healing in the ozone group was significantly higher than in the other groups. We found that subcutaneous ozone therapy was more effective than silver sulfadiazine in the healing process of second-degree burn wounds and could be safely used in the treatment of burn wounds.

scholarly journals Terapi Oksigen Hiperbarik Meningkatkan Kualitas Hidup Pasien Glioblastoma Multiforme Stadium Akhir Dinilai Berdasarkan Neurologic Assessment Neuro Oncology Score

e-CliniC ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 556
Author(s):  
Hanni Djunadi ◽  
Maximillian Ch. Oley ◽  
Eko Prasetyo ◽  
Mendy Hatibie ◽  
Fredrik G. Langi
Keyword(s):  
Glioblastoma Multiforme ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Healing Process ◽  
Hypoxic Condition ◽  
Wound Healing Process ◽  
Before And After ◽  
End Stage ◽  
Surgery Department

Abstract: Patients with end-stage glioblastoma multiforme (GBM) that cannot be operated or treated with chemotheraphy and radiotheraphy have increased clinical complaints, thus affecting the patients’ quality of lifes (QoL). Hyperbaric oxygen therapy (HBOT) could alters hypoxic condition in tumor tissue with a different cascade from wound healing process. This study was aimed to assess whether the QoL of end-stage GBM patients could improve with the administration of HBOT assessed by decreased clinical complaints based on NANO score. The study was carried out at the Surgery Department of Prof. Dr. R. D. Kandou Hospital Manado, using pre and posttrial design. Each sample was assessed for the NANO score before HBOT, after one time, three times, five times, and 10 times of HBOT. The results showed that there was a change in the NANO score before and after 10 times of HBOT. The decrease in the NANO score occurred more quickly after the therapy. In the last two measurements, the NANO score according to the RVC model decreased from one to two units. The decreased NANO score occurred quite regularly with variations in the score that changed from time to time. In conclusion, HBOT can improve the QoL of patients with end-stage GBM and reduce the NANO score which is an assessment of clinical complaints of the patients.Keywords: glioblastoma multiforme; hyperbaric oxygen therapy; NANO score Abstrak: Penderita glioblastoma multiforme (GBM) stadium akhir yang tidak dapat dilakukan operasi, kemoterapi dan radioterapi memiliki keluhan klinis yang semakin meningkat sehingga memengaruhi kualitas hidup. Terapi oksigen hiperbarik (TOHB) dapat mengubah kondisi hipoksia pada jaringan tumor dengan kaskade yang berbeda dari proses penyembuhan luka. Penelitian ini bertujuan untuk menilai apakah kualitas hidup pasien GBM stadium akhir dapat membaik dengan pemberian TOHB, dinilai dengan menurunnya keluhan klinis berdasarkan NANO score. Penelitian dilakukan di Bagian Bedah RSUP Prof. Dr. R. D. Kandou Manado, dengan menggunakan pre and posttrial design. Pada setiap sampel dilakukan penilaian NANO score sebelum TOHB, setelah satu kali, tiga kali, lima kali, dan 10 kali TOHB. Hasil penelitian mendapatkan adanya perubahan NANO score sebelum TOHB dan setelah 10 kali TOHB. Penurunan NANO score berlangsung lebih cepat pasca terapi tersebut. Dalam dua pengukuran terakhir, NANO score menurut model RVC turun dari satu hingga dua satuan. Pada grafik, hasil penurunan NANO score terjadi cukup beraturan dengan variasi score yang berubah dari waktu ke waktu. Simpulan penelitian ini ialah TOHB dapat meningkatkan kualitas hidup penderita GBM stadium akhir dan menurunkan NANO score yang menjadi penilainan keluhan klinis penderita.Kata kunci: glioblastoma multiforme; terapi oksigen hiperbarik; NANO score

scholarly journals The Efficacy of Silver-Based Electrospun Antimicrobial Dressing in Accelerating the Regeneration of Partial Thickness Burn Wounds Using a Porcine Model

Polymers ◽  
2021 ◽  
Vol 13 (18) ◽  
pp. 3116
Author(s):  
Thien Do ◽  
Tien Nguyen ◽  
Minh Ho ◽  
Nghi Nguyen ◽  
Thai Do ◽  
...  
Keyword(s):  
Wound Healing ◽  
Burn Injury ◽  
Healing Process ◽  
Bactericidal Effect ◽  
Wound Healing Process ◽  
Burn Wounds ◽  
Partial Thickness Burn ◽  
Tissue Damages

(1) Background: Wounds with damages to the subcutaneous are difficult to regenerate because of the tissue damages and complications such as bacterial infection. (2) Methods: In this study, we created burn wounds on pigs and investigated the efficacy of three biomaterials: polycaprolactone-gelatin-silver membrane (PCLGelAg) and two commercial burn dressings, Aquacel® Ag and UrgoTulTM silver sulfadiazine. In vitro long-term antibacterial property and in vivo wound healing performance were investigated. Agar diffusion assays were employed to evaluate bacterial inhibition at different time intervals. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time-kill assays were used to compare antibacterial strength among samples. Second-degree burn wounds in the pig model were designed to evaluate the efficiency of all dressings in supporting the wound healing process. (3) Results: The results showed that PCLGelAg membrane was the most effective in killing both Gram-positive and Gram-negative bacteria bacteria with the lowest MBC value. All three dressings (PCLGelAg, Aquacel, and UrgoTul) exhibited bactericidal effect during the first 24 h, supported wound healing as well as prevented infection and inflammation. (4) Conclusions: The results suggest that the PCLGelAg membrane is a practical solution for the treatment of severe burn injury and other infection-related skin complications.

Sign in / Sign up

Export Citation Format

Share Document