scholarly journals Aksi Ethyl Methane Sulphonate terhadap Munculnya Bibit dan Pertumbuhan Cabai Rawit(Capsicum frutescens L.) (Ethyl Methane Sulphonate Action on Seed Emergence and Growth of (Capsicum frutecens L.))

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Ni Kadek Dewi Rustini ◽  
Made Pharmawati
Keyword(s):  
Plant Height ◽  
Phosphate Buffer ◽  
Seedling Emergence ◽  
Capsicum Frutescens ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Methane Sulphonate ◽  
Treated Seed ◽  
Growth Characters

Abstrak Penelitian ini bertujuan mengamati pengaruh lama perendaman biji dengan 1% EMS terhadap persentase munculnya bibit dan karakter pertumbuhan tanaman cabai rawit pada umur 4 minggu setelah tanam (MST). Benih cabai rawit direndam dalam air selama 6 jam, selanjutnya direndam 1% EMS dalam buffer fosfat pH 7, selama 6 jam, 9 jam dan 12 jam. Tiap biji disemai dalam bumbungan kertas. Munculnya bibit diamati setiap hari. Setelah berumur 3 minggu, bibit dipindahkan ke bedengan. Hasil menunjukkan perlakuan 1% EMS memperlambat munculnya bibit. Pada 10 hari setelah semai (HSS) munculnya bibit pada perlakuan dapat mencapai 100%. Perlakuan 1% EMS berpengaruh nyata terhadap tinggi tanaman dan jumlah daun, sedangkan panjang, dan lebar daun dengan perendaman 6 jam tidak berbeda dengan kontrol, namun berbeda dengan perendaman 9 jam dan 12 jam. Kata kunci : EMS, bibit, pertumbuhan, Capsicum frustescens L.   Abstract This research aimed to evaluate the influence of different exposure durations of 1% EMS on seedling emergence and growth characters of C, fustescent at 4 week after planting (WAT). Seeds were soaked in water for 6 hours, then soaked in 1% EMS in phosphate buffer pH 7, for 6 hours, 9 hours and 12 hours. Each seed was then sowed in a single paper tube. Seedling emergence was observed every day. At 3 weeks after sowing, seedlings were transferred to field. Results showed that soaking seeds in 1% EMS inhibited seedling emergence. At 10 days after sowing, the percentages of seedling emergence at control and treated seed were 100%. Treatments of 1% EMS have a significant effect on plant height, and the number of leaf, while length, and width of leaf with 6 hour exposure were not different with control, but differ from exposure of 9 hours and 12 hours. Keywords: EMS, seed emergence, growth, Capsicum frutescens L.

scholarly journals Morphology and Yield Parameters and Biochemical Analysis of Soybean (Glycine max (L.) Mrr.) Using Gamma Rays, EMS and DES Treatment

2015 ◽  
Vol 35 ◽  
pp. 50-58 ◽  
Author(s):  
P. Gopinath ◽  
P. Pavadai
Keyword(s):  
Glycine Max ◽  
Plant Height ◽  
Seed Yield ◽  
Gamma Rays ◽  
Fruit Size ◽  
Mean Value ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Yield Parameters ◽  
Methane Sulphonate

Soybean (Glycine max (L.) Merr.) var. Co-1 seeds were treated with physical mutagen namely such as gamma rays and chemical mutagen namely such as Ethyl Methane Sulphonate (EMS) and Diethyl Sulphate (DES). Various concentrations of gamma rays 10, 20, 30, 40, 50 and 60 KR, EMS (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %) and DES (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %). The morphological parameters such as days to first flower, plant height, number of cluster per plant, number of pod per plan, seed yield per plant, number of branches per plant, number of leaves per plant, protein content and oil content increasing with increasing level of some doses gamma rays, Ethyl methane sulphonate and Diethyl sulphate treatment in M2 and M3 generation were carried out. A number of mutants were recorded in M2 and M3 generations for one or more traits viz., plant height, maturity, branching, pigmentation, fruit size and yield were isolated in M2 and M3 generation. Mean for various characters increased at mutagenic treatment than control. The yield parameters like plant height, number of cluster per plant, number of seeds per plant and seed yield per plant were recorded the moderated and high mean value in the 50 KR of gamma rays 0.5% of EMS and 0.4% of DES treated population with compared to control plants.

scholarly journals Analysis of Genetic Parameters on Mutant Populations of Mungbean (Vigna radiata L.) after Ethyl Methane Sulphonate Treatment

2012 ◽  
Vol 4 (1) ◽  
pp. 137-143 ◽  
Author(s):  
Rajib ROYCHOWDHURY ◽  
Sudipta DATTA ◽  
Parineeta GUPTA ◽  
Jagatpati TAH
Keyword(s):  
Genetic Variability ◽  
Plant Height ◽  
Vigna Radiata ◽  
Genetic Parameters ◽  
Heritability Estimate ◽  
Pure Line ◽  
Genetic Advance ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Methane Sulphonate

In the present study, pure line seeds of mungbean (Vigna radiata L. Wilczek) were treated with four doses (0.2%, 0.4%, 0.6% and 0.8% as w/v) of ethyl methane sulphonate (EMS) to analyse the genetic variability and heritability for some prime agro-metrical traits in second mutant (M2) generation. The analysis of variance revealed highly significant differences among the studied characters. The phenotypic coefficient of variation (PCV) was higher than its genotypic counterpart (GCV) for all the studied traits. The highest GCV (0.537) for number of seeds per pods and highest PCV (0.635) for plant height were recorded in 0.4% EMS treatment. The lowest GCV (0.179) and PCV (0.214) were recorded in the control set for number of pods per plant. The highest broad sense heritability estimate (92.33%) was recorded on 0.4% EMS for plant height. The expected genetic advance was high (42.39%) in 0.4% dose for days to flowering. The lowest heritability (38.43) and genetic advance (5.37) were noticed in control for the total seed yield per plant. EMS at 0.4% and 0.6% concentration gave the maximum values of all the genetic parameters. The increased genetic variability in treated population for these traits has a high scope for selection and can be exploited for the further improvement of mungbean.

Evaluation of Yield Attributing Variants Developed Through Ethyl Methane Sulphonate in an Important Proteinaceous Crop-Vicia faba

2017 ◽  
Vol 9 (2) ◽  
pp. 20-27 ◽  
Author(s):  
Durre Shahwar ◽  
Mohammad Yunus Khali Ansari ◽  
Sana Chaudhary ◽  
Rumana Aslam
Keyword(s):  
Vicia Faba ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Methane Sulphonate

Effect of seed treatment by ethyl methane sulphonate on growth, flowering and yield of papaya cv. Pusa Dwarf

2016 ◽  
Vol 7 (1) ◽  
pp. 64
Author(s):  
Mahesh Kumar ◽  
Mukesh Kumar ◽  
Satya Prakash ◽  
DK Gautam ◽  
Sanjeev Rao
Keyword(s):  
Seed Treatment ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Methane Sulphonate

scholarly journals Increased sensitivity to cell killing and mutagenesis by chemical mutagens in thymidine-kinase-deficient subclones of a Friend murine leukaemia cell line

1982 ◽  
Vol 40 (2) ◽  
pp. 207-212 ◽  
Author(s):  
P. G. McKenna ◽  
A. A. Yasseen
Keyword(s):  
Thymidine Kinase ◽  
Wild Type ◽  
Ethyl Methane Sulphonate ◽  
Leukaemia Cell ◽  
Leukaemia Cell Line ◽  
Ethyl Methane ◽  
Chemical Mutagens ◽  
Methane Sulphonate ◽  
Increased Sensitivity ◽  
Unit Dose

SUMMARYWild-type Friend murine leukaemia (clone 707) cells and two thymidinekinase-deficient subclones, 707BUE and 707BUF, were compared for sensitivity to killing and mutagenesis by the chemical mutagens, ethyl methane sulphonate (EMS),N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), mitomycin C (MMC), and methyl methane sulphonate (MMS). The two thymidine-kinase-deficient subclones were significantly more sensitive to killing by each of the four chemical mutagens than were wild-type cells. The increased sensitivity to killing by the four mutagens was also reflected in increased mutagenesis (per unit dose of mutagen) to 6-thioguanine resistance. In the light of these results, the significance of thymidine kinase in DNA repair and mutagenesis is discussed.

Quantitative variation induced by gamma rays, ethyl methane sulphonate, and hydrazine hydrate in mung bean (Phaseolus aureus Roxb.)

1984 ◽  
Vol 26 (4) ◽  
pp. 492-496 ◽  
Author(s):  
Irfan A. Khan
Keyword(s):  
Gamma Rays ◽  
Seed Weight ◽  
Genetic Advance ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Plant Yield ◽  
Mean Values ◽  
Methane Sulphonate ◽  
The Mean ◽  
100 Seed Weight

Induced variability was studied in the quantitative characters of mung bean (Phaseolus aureus Roxb.) after treatment with gamma rays, ethyl methane sulphonate (EMS), and hydrazine hydrate (HZ) in M1, M2, and M3 generations. The characters evaluated were seeds per pod, 100-seed weight, and total plant yield. The variability increased in almost all of the characters in the M1 generation. The mean number of seeds per pod and 100-seed weight decreased, whereas the plant yield did not show any particular trend. There was an increase in the mean values after gamma irradiation and EMS treatments in the M2 generation. However, HZ fails to show the same response. However, the mean values increased in the M3 generation. Estimates of heritability were higher for 100-seed weight, followed by plant yield, and then seeds per pod in the M3 generation. Coefficient of genotypic variation and genetic advance were high for the plant yield in M2 and M3 generations. In general, the genotypic coefficient of variation values, heritability, and genetic advance increased more in M3 as compared with M2, indicating that the significant gain could possibly be achieved through selection in M3 generation.Key words: quantitative, heritability, mutagenesis.

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