scholarly journals EFFECTS OF MODERN SEED PROTECTANTS ON VIABILITY AND FUNCTIONAL ACTIVITY OF BIOLOGICAL AGENTS OF MICROBIAL PREPARATIONS

2014 ◽  
Vol 18 ◽  
pp. 120-131
Author(s):  
L.М. Tokmakova ◽  
Yu.О. Tararyko ◽  
А.O. Trepach ◽  
О.P. Lepekhа ◽  
І.V. Larchenko

The influence of seed protectants crop viability and functional activity of bacterial cells - biological agents microbial preparations. The possibility of the use of protectants compatible with biological products, bio-agents which exhibit resistance to the pesticide active ingredient.

2021 ◽  
Vol 33 ◽  
pp. 33-43
Author(s):  
Kozar S. F.

Objective. Investigate approaches to managing the activity of soil diazotrophs and propose a strategy for its regulation. Methods. Theoretical, vegetation and field experiments, microbiological, gas chromatographic, mathematical and statistical. Results. The activity of beneficial soil microorganisms can change under the action of temperature, humidity, chemical compounds of various origin, and other microorganisms. It was established that, taking into account a significant variety of factors, it is necessary to develop a set of specific ways to increase the growth and functional activity of nitrogen-fixing bacteria, as well as their viability. It has been proved that the combination of diazotrophs forms an effective symbiotic leguminous-rhizobial system, which provides additional biological nitrogen in agrocenoses. At the same time, there was an increase in plant mass, chlorophyll content in the leaves, protein and oil content in the products. The combined use of diazotrophs increases the yield, in particular, soybeans by 9–16 % compared with inoculation by pure bacterial culture. Conclusion. Based on the analysis and generalization of the obtained research results, a strategy for regulating the activity of diazotrophs for their effective introduction into agrocenoses is proposed, which consists in combining bacteria of different species, selecting conditions for their co-cultivation and application upon stabilisation of the number of viable bacterial cells. The proposed strategy involves solving the problem by obtaining an inoculant, which is characterized by a high titre and a stable number of viable cells, which allows to obtain an effective nitrogen-fixing system. The strategy is tried-and-tested on the example of regulating the growth and functional activity of soybean nodule bacteria by combining diazotrophs of different species, substantiating the conditions of their co-cultivation and application to ensure positive interaction in the form of commensalism, as well as by regulating viability of diazotrophs by adding stabilisers to the medium.


2020 ◽  
Vol 23 (1) ◽  
pp. 39-41
Author(s):  
A. R. Tavitova ◽  
A. A. Krinitsyna ◽  
K. M. Lomonosov

A literature review is presented regarding the vitiligo reaction to biological agents for treating this dermatosis itself and as a side effect of the treatment of other diseases. Notably, the results of therapy with biological products and the researchers opinions on this subject are ambiguous because of the positive evidence of repigmentation of vitiligo foci, as well as cases of vitiligo in patients who received biological products for the treatment of another pathology.


Blood ◽  
1986 ◽  
Vol 67 (2) ◽  
pp. 257-267 ◽  
Author(s):  
D Metcalf

Abstract Rapid progress has occurred recently in characterizing the molecular nature of the specific glycoprotein colony-stimulating factors (CSFs) controlling the proliferation; and some functional activities of granulocytes and monocyte-macrophages. All four known murine CSFs have been purified, and cDNAs for two have been cloned and expressed by mammalian and bacterial cells. Similarly, three human CSFs have been purified, and cDNAs for two cloned and expressed. This work has opened up the exciting prospects of testing the effects of these recombinant CSFs on hematopoiesis in vivo. Each CSF has a broader range of hematopoietic target cells than previously suspected, and it is now clear that the CSFs are not simply proliferative stimuli but can also regulate the functional activity of mature cells. There are increasing reasons to believe that these CSFs will be useful therapeutic agents in stimulating hematopoietic regeneration in leukopenic states and the functional activity of granulocytes and monocytes in infections.


2007 ◽  
Vol 4 ◽  
pp. 7-20
Author(s):  
V.P. Patyka ◽  
N.V. Patyka

The results of investigations of Ukrainian and Russian scientists- microbiologists as for creation of microbic preparations and their ap¬plication in bioorganic agriculture are generalized. The major factors and the scheme of biotechnological manufacturing of specified biologi¬cal products are considered. It has been shown, that one of the most important properties of the preparation is the form of manufacturing. Searching of universal fills is very actual as well as different updatings of biological products which provide maximal adaptibility of biological agents to specific conditions, depending on regions. For the first time gel, lignin and vermiculturas forms of biological products based on new strains of nitrogen fixing bacteria have been developed by Ukrainian scientists.


Blood ◽  
1986 ◽  
Vol 67 (2) ◽  
pp. 257-267 ◽  
Author(s):  
D Metcalf

Rapid progress has occurred recently in characterizing the molecular nature of the specific glycoprotein colony-stimulating factors (CSFs) controlling the proliferation; and some functional activities of granulocytes and monocyte-macrophages. All four known murine CSFs have been purified, and cDNAs for two have been cloned and expressed by mammalian and bacterial cells. Similarly, three human CSFs have been purified, and cDNAs for two cloned and expressed. This work has opened up the exciting prospects of testing the effects of these recombinant CSFs on hematopoiesis in vivo. Each CSF has a broader range of hematopoietic target cells than previously suspected, and it is now clear that the CSFs are not simply proliferative stimuli but can also regulate the functional activity of mature cells. There are increasing reasons to believe that these CSFs will be useful therapeutic agents in stimulating hematopoietic regeneration in leukopenic states and the functional activity of granulocytes and monocytes in infections.


2020 ◽  
Vol 43 ◽  
Author(s):  
David Spurrett

Abstract Comprehensive accounts of resource-rational attempts to maximise utility shouldn't ignore the demands of constructing utility representations. This can be onerous when, as in humans, there are many rewarding modalities. Another thing best not ignored is the processing demands of making functional activity out of the many degrees of freedom of a body. The target article is almost silent on both.


Author(s):  
R. H. Liss

Piperacillip (PIP) is b-[D(-)-α-(4-ethy1-2,3-dioxo-l-piperzinylcar-bonylamino)-α-phenylacetamido]-penicillanate. The broad spectrum semisynthetic β-lactam antibiotic is believed to effect bactericidal activity through its affinity for penicillin-binding proteins (PBPs), enzymes on the bacterial cytoplasmic membrane that control elongation and septation during cell growth and division. The purpose of this study was to correlate penetration and binding of 14C-PIP in bacterial cells with drug-induced lethal changes assessed by microscopic, microbiologic and biochemical methods.The bacteria used were clinical isolates of Escherichia coli and Pseudomonas aeruginosa (Figure 1). Sensitivity to the drug was determined by serial tube dilution in Trypticase Soy Broth (BBL) at an inoculum of 104 organisms/ml; the minimum inhibitory concentration of piperacillin for both bacteria was 1 μg/ml. To assess drug binding to PBPs, the bacteria were incubated with 14C-PIP (5 μg/0.09 μCi/ml); controls, in drug-free medium.


Author(s):  
J. E. Laffoon ◽  
R. L. Anderson ◽  
J. C. Keller ◽  
C. D. Wu-Yuan

Titanium (Ti) dental implants have been used widely for many years. Long term implant failures are related, in part, to the development of peri-implantitis frequently associated with bacteria. Bacterial adherence and colonization have been considered a key factor in the pathogenesis of many biomaterial based infections. Without the initial attachment of oral bacteria to Ti-implant surfaces, subsequent polymicrobial accumulation and colonization leading to peri-implant disease cannot occur. The overall goal of this study is to examine the implant-oral bacterial interfaces and gain a greater understanding of their attachment characteristics and mechanisms. Since the detailed cell surface ultrastructure involved in attachment is only discernible at the electron microscopy level, the study is complicated by the technical problem of obtaining titanium implant and attached bacterial cells in the same ultra-thin sections. In this study, a technique was developed to facilitate the study of Ti implant-bacteria interface.Discs of polymerized Spurr’s resin (12 mm x 5 mm) were formed to a thickness of approximately 3 mm using an EM block holder (Fig. 1). Titanium was then deposited by vacuum deposition to a film thickness of 300Å (Fig. 2).


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