scholarly journals Effect of complete and partial capsulotomy on the renal function tests and oxidative stress markers in rats undergoing ischemia-reperfusion injury

2019 ◽  
Vol 20 (4) ◽  
pp. 29-36
Author(s):  
Yomna Khater ◽  
Awad Rizk ◽  
Mohamed Saad ◽  
Adel Zaghloul
Keyword(s):  
Oxidative Stress ◽  
Renal Function ◽  
Serum Creatinine ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Post Surgery ◽  
Renal Function Tests ◽  
And Oxidative Stress

Objective: To compare the effect of complete and partial renal capsulotomy on the renal function tests and oxidative stress markers in rats undergoing ischemia-reperfusion injury. Design: Randomized controlled experimental study. Animals: A 60 Spraque-dawely rats weighing 180 ± 50 g. Procedures: Rats were divided into 3 groups in triplicate (6 each). In addition, 6 rats were subjected to blood and renal tissues sampling for estimation of normal parameters. Group 1 (Positive control): ischemia reperfusion (IR) injury; Group 2: Complete capsulotomy + I R; Group 3: Partial capsulotomy + IR. Six rats from each group were sacrified at 2, 7 and 14 days post- surgery. Results: The complete capsulotomy induced a significant decrease in the serum creatinine at 2 and 7 days post- capsulotomy in comparison with partial capsulotomy (P < 0.05), whereas at 14 days, the partial capsulotomy induced the significant decrease (P < 0.05). Complete capsulotomy showed a significant improvement in creatinine clearance in comparasion with partial capsulotomy at 2, 7 and 14 days post- surgery (p<0.05). At 2 and 7 days, BUN of IR+ Capsulotomy group showed a significant decrease (P < 0.05) compared to the other groups, while at 14 days partial capsulotomy, the serum BUN reached to the normal value. Serum sodium level showed a significant decrease (P < 0.05) at 2 days after partial capsulotomy, and at 14 days after complete capsulotomy (P < 0.05). Nitric oxide level in IR + partial capsulotomy group showed a significant decrease at 7 and 14 days (P < 0.05). Results of MDA of IR+ partial capsulotomy groups showed a significant decrease (P < 0.05) compared to the IR+ compete capsulotomy groups at 2,7 and 14 days. Conclusion and clinical relevance: The partial capsulotomy ameliorates could improve serum creatinine, BUN and could lower the oxidative stress at 14 days. Partial capsulotomy could also improve the renal tissues at both short and long-term. So this study indicates the importance of the presence of intact renal capsule for ischemic acute kidney injury.

scholarly journals Assessment of Oxidative Stress Markers in Hypothermic Preservation of Transplanted Kidneys

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1263
Author(s):  
Karol Tejchman ◽  
Anita Sierocka ◽  
Katarzyna Kotfis ◽  
Maciej Kotowski ◽  
Barbara Dolegowska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Kidney Function ◽  
Blood Count ◽  
Glutathione Transferase ◽  
Ischemia Reperfusion ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Group 2 ◽  
Transplanted Kidneys ◽  
And Oxidative Stress

Ischemia-reperfusion injury (IRI) after renal transplantation is a complex biochemical process. The first component is an ischemic phase during kidney storage. The second is reperfusion, the main source of oxidative stress. This study aimed to analyze the activity of enzymes and concentrations of non-enzymatic compounds involved in the antioxidant defense mechanisms: glutathione (GSH), glutathione peroxidase (GPX), catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione transferase (GST), thiobarbituric acid reactive substances (TBARS), malondialdehyde (MDA), measured in preservation fluid before transplantation of human kidneys (KTx) grafted from brain dead donors. The study group (N = 66) was divided according to the method of kidney storage: Group 1—hypothermic machine perfusion (HMP) in LifePort perfusion pump, n1 = 26, and Group 2—static cold storage (SCS), n2 = 40. The measurements of kidney function parameters, blood count, and adverse events were performed at constant time points during 7-day hospitalization and 3-month follow-up. Kidney perfusate in Group 2 was characterized by significantly more acidic pH (p < 0.0001), higher activity of GPX [U/mgHb] (p < 0.05) and higher concentration of MDA [μmol/L] (p < 0.05). There was a statistically significant improvement of kidney function and specific blood count alterations concerning storage method in repeated measures. There were aggregations of significant correlations (p < 0.05) between kidney function parameters after KTx and oxidative stress markers: diuresis & CAT, Na+ & CAT, K+ & GPX, urea & GR. There were aggregations of significant correlations (p < 0.05) between recipient blood count and oxidative stress markers: CAT & MON, SOD & WBC, SOD & MON. Study groups demonstrated differences concerning the method of kidney storage. A significant role of recipient’s gender, gender matching, preservation solution, and perfusate pH was not confirmed, however, basing on analyzed data, the well-established long-term beneficial impact of HMP on the outcome of transplanted kidneys might partially depend on the intensity of IRI ischemic phase and oxidative stress, reflected by the examined biomarkers.

Comparison of Salivary Antioxidants and Oxidative Stress Status in Gestational Diabetes Mellitus and Healthy Pregnant Women

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Shima Fathi ◽  
Mohammad Taghi Goodarzi ◽  
Shiva Borzouei ◽  
Jalal Poorolajal ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Pregnant Women ◽  
Oxidative Stress Markers ◽  
Blood Samples ◽  
Stress Markers ◽  
Total Antioxidant ◽  
Total Oxidative Stress ◽  
And Oxidative Stress

Background: One of the most common complications of pregnant women is gestational diabetes mellitus (GDM). Oxidative stress can play an important role in GDM. Objective: The aim of this study was to evaluate salivary antioxidants and oxidative stress markers in GDM. Method: Twenty pregnant women with GDM and 20 healthy pregnant women with normal blood glucose test participated in this study. Five mL of unstimulated saliva samples were collected. Spectrophotometric assay was carried out for sialochemical analysis. Stata software was used for data analysis. Results: The GDM group exhibited no significant difference in salivary total antioxidant capacity and malondialdehyde compared to the healthy control group. All of antioxidants markers, the uric acid, total antioxidant, peroxidase and catalase, decreased in GDM group that the difference of peroxidase and catalase was statistically significant. All of oxidative stress markers, the salivary malondyaldehid, total oxidative stress and total thiol, increased in GDM group. GDM group exhibited significantly higher salivary total oxidative stress levels. Conclusion: Catalase level was significantly lower and total oxidative stress was significantly higher. These two markers might have significant importance and might exhibit early changes compared to other factors in GDM. . Some of salivary antioxidants might have diagnostic, prognostic or therapeutic implications in GDM. Other studies with large sample size on salivary and blood samples need to be done to confirm this properties and salivary samples using instead of blood samples in GDM biomarkers changes.

Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Type 2 Diabetes Mellitus: A Retrospective Cohort Study

2020 ◽  
Vol 20 (4) ◽  
pp. 584-590 ◽  
Author(s):  
Shima Fathi ◽  
Shiva Borzouei ◽  
Mohammad Taghi Goodarzi ◽  
Jalal Poorolajal ◽  
Fatemeh Ahmadi-Motamayel
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Type 2 Dm ◽  
Patients With Diabetes ◽  
The Difference ◽  
And Oxidative Stress

Background: Diabetes Mellitus (DM) is a progressive metabolic disorder. Objective: The aim of this study was to investigate the relationship between antioxidant and oxidative stress markers in the saliva of patients with type 2 DM and a healthy control group. Methods: In this study, 20 patients with diabetes and 20 healthy individuals were evaluated. Salivary antioxidants markers consisted of total antioxidant capacity (TAC), uric acid (UA), peroxidase and catalase. Oxidative stress markers included total oxidant status (TOS), malondealdehyde (MDA) and total thiol (SH). Sialochemical analysis was performed with spectrophotometric assay. All the statistical analyses were conducted using STATA software. Results: TAC decreased significantly in patients with diabetes. Although salivary UA and peroxidase were lower in patients with diabetes compared to the control group, the difference was not significant. Salivary catalase in patients with diabetes was significantly lower than that in the control group. MDA and TOS exhibited significantly higher levels in type 2 DM. SH levels were slightly higher in DM. Conclusions: According to the results of the present study, there were some changes in the salivary levels of some antioxidants and oxidative stress markers in patients with type 2 DM and could be measured as an indicator of serum changes..

Minocycline effects on cerebral edema: Relations with inflammatory and oxidative stress markers following traumatic brain injury in mice

2009 ◽  
Vol 1291 ◽  
pp. 122-132 ◽  
Author(s):  
Shadi Homsi ◽  
Fabiola Federico ◽  
Nicole Croci ◽  
Bruno Palmier ◽  
Michel Plotkine ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Cerebral Edema ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
And Oxidative Stress
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