A STUDY TO CORRELATE HISTOPATHOLOGY, SERUM PSA LEVEL AND IMMUNOHISTOCHEMICAL EXPRESSION OF ER & PR IN PROSTATIC GROWTH

Objectives- Prostate is a bromusculoglandular structure situated at the neck of urinary bladder. It enlarges due to benign hyperplasia of prostate (BHP), prostatic intraepithelial neoplasia (PIN) or adenocarcinoma. Enlargement of prostate is associated with raised serum level of prostate-specic antigen (PSA) and altered expression of estrogen receptor (ER) and progesterone receptor (PR). The aim of our study is to correlate the histopathology, PSA levels and altered expression of ER and PR by immunohistochemistry in different prostatic growth lesions. Methodology- Patients diagnosed as having prostatic growth were enrolled and their serum PSA levels were noted. Histopathological examination and immunohistochemical analysis of prostatic tissues for ER and PR were carried out to nd out correlation of different type of growth with serum PSA level and expression of ER and PR. Results- A total 96 cases studied of them 61(63.54%) patients presented with BHP, 20(20.83%) patients with BHP with chronic prostatitis, 3 patients presented with metaplastic changes, 5 cases with of PIN and 6 patients presented with adenocarcinoma with different Gleason score. PR expression positivity in epithelial cells and stromal cells of BHP cases were 51(83.6%) and 53(86.88%) respectively. Patients presented with adenocarcinoma showed only 33.33 %( 2cases) positivity in epithelial cells and 50% (3cases) positivity in stromal cells. Serum PSA level were signicantly higher in adenocarcinoma patients as compare to BHP patients. Conclusion- By observing these ndings it can be suggested that and antiprogesterone therapy may be helpful in the treatment of prostatic adenocarcinoma.

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Glyoxalase 1 Expression as a Novel Diagnostic Marker of High-Grade Prostatic Intraepithelial Neoplasia in Prostate Cancer

Cancers ◽

10.3390/cancers13143608 ◽

2021 ◽

Vol 13 (14) ◽

pp. 3608

Author(s):

Liliana Rounds ◽

Ray B. Nagle ◽

Andrea Muranyi ◽

Jana Jandova ◽

Scott Gill ◽

...

Keyword(s):

Prostate Cancer ◽

Diagnostic Marker ◽

Precancerous Lesion ◽

Immunohistochemical Analysis ◽

Intraepithelial Neoplasia ◽

Prostatic Intraepithelial Neoplasia ◽

Glycolytic Flux ◽

Gleason Grade ◽

High Grade ◽

Glyoxalase 1

Glyoxalase 1 (GLO1) is an enzyme involved in the detoxification of methylglyoxal (MG), a reactive oncometabolite formed in the context of energy metabolism as a result of high glycolytic flux. Prior clinical evidence has documented GLO1 upregulation in various tumor types including prostate cancer (PCa). However, GLO1 expression has not been explored in the context of PCa progression with a focus on high-grade prostatic intraepithelial neoplasia (HGPIN), a frequent precursor to invasive cancer. Here, we have evaluated GLO1 expression by immunohistochemistry in archival tumor samples from 187 PCa patients (stage 2 and 3). Immunohistochemical analysis revealed GLO1 upregulation during tumor progression, observable in HGPIN and PCa versus normal prostatic tissue. GLO1 upregulation was identified as a novel hallmark of HGPIN lesions, displaying the highest staining intensity in all clinical patient specimens. GLO1 expression correlated with intermediate–high risk Gleason grade but not with patient age, biochemical recurrence, or pathological stage. Our data identify upregulated GLO1 expression as a molecular hallmark of HGPIN lesions detectable by immunohistochemical analysis. Since current pathological assessment of HGPIN status solely depends on morphological features, GLO1 may serve as a novel diagnostic marker that identifies this precancerous lesion.

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Spectrum of prostate gland lesions: a clinico-pathologic study in south-western Bihar, India

International Journal of Advances in Medicine ◽

10.18203/2349-3933.ijam20192258 ◽

2019 ◽

Vol 6 (3) ◽

pp. 885

Author(s):

Rajendra Kumar ◽

Wakeel Ahmad

Keyword(s):

Chronic Prostatitis ◽

Operative Procedure ◽

Prostate Gland ◽

Intraepithelial Neoplasia ◽

Prostatic Intraepithelial Neoplasia ◽

Pathological Changes ◽

Malignant Tumours ◽

Age Related ◽

Nodular Hyperplasia ◽

First Time

Background: Prostate gland is an endocrine dependent organ in males and age-related lesions involve it. Inflammation, benign nodular hyperplasia and tumours are the commonest to involve it worldwide. Occasionally some other pathological changes can also involve it. The study was carried out for the first time to know the spectrum and prevalence of prostate lesions which will be of help to the clinicians in this location.Methods: Retrospective study was carried out for a period of four years and out of surgical cases of males which underwent operative procedure, prostate cases were retrieved and in the department of pathology, haematoxylin and eosin stained slides were evaluated and diagnosed.Results: A total of 138 cases were included and five cases were excluded from this series. Benign nodular hyperplasia (73.9%) was the commonest finding followed by chronic prostatitis associated with hyperplasia (15.2%), atypical glandular hyperplasia (2.9%) and prostatic intraepithelial neoplasia (2.1%). Malignant tumours were 5.8% constituting adenocarcinoma prostate as the most common (62.5%). A case of hemangiopericytoma was also diagnosed.Conclusions: Benign nodular hyperplasia of prostate is the most common affliction among males starting at age 45 years. Early consultation and screening will be of immense value. Adenocarcinoma prostate may involve at age around 58 years.

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Editorial Comment on: Immunohistochemical Expression of Endothelin-1 and Endothelin-A and Endothelin-B Receptors in High-Grade Prostatic Intraepithelial Neoplasia and Prostate Cancer

European Urology ◽

10.1016/j.eururo.2007.02.025 ◽

2007 ◽

Vol 52 (6) ◽

pp. 1689-1690

Author(s):

Lars Egevad

Keyword(s):

Prostate Cancer ◽

Editorial Comment ◽

Intraepithelial Neoplasia ◽

Prostatic Intraepithelial Neoplasia ◽

High Grade ◽

Immunohistochemical Expression ◽

Endothelin 1 ◽

Endothelin A ◽

Endothelin B

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Immunohistochemical Expression of Endothelin-1 and Endothelin-A and Endothelin-B Receptors in High-Grade Prostatic Intraepithelial Neoplasia and Prostate Cancer

European Urology ◽

10.1016/j.eururo.2007.02.024 ◽

2007 ◽

Vol 52 (6) ◽

pp. 1682-1690 ◽

Cited By ~ 19

Author(s):

Rodolfo Montironi ◽

Roberta Mazzucchelli ◽

Francesca Barbisan ◽

Daniela Stramazzotti ◽

Alfredo Santinelli ◽

...

Keyword(s):

Prostate Cancer ◽

Intraepithelial Neoplasia ◽

Prostatic Intraepithelial Neoplasia ◽

High Grade ◽

Immunohistochemical Expression ◽

Endothelin 1 ◽

Endothelin A ◽

Endothelin B

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A Case of Solitary Nonvascularized Corneal Epithelial Dysplasia

Case Reports in Ophthalmological Medicine ◽

10.1155/2016/5687285 ◽

2016 ◽

Vol 2016 ◽

pp. 1-4

Author(s):

Tomoya Morii ◽

Takayoshi Sumioka ◽

Ai Izutani-Kitano ◽

Yukihisa Takada ◽

Yuka Okada ◽

...

Keyword(s):

Epithelial Cells ◽

Corneal Epithelium ◽

Ocular Surface ◽

Histopathological Examination ◽

Precancerous Lesion ◽

Intraepithelial Neoplasia ◽

Epithelial Dysplasia ◽

Corneal Epithelial ◽

Keratin 13 ◽

Keratin 12

Background. Epithelial dysplasia is categorized as conjunctival/corneal intraepithelial neoplasia which is a precancerous lesion. The lesion is usually developed at the limbal region and grows towards central cornea in association with neovascularization into the lesion. Here, we report a case of isolated nonvascularized corneal epithelial dysplasia surrounded by normal corneal epithelium with immune histochemical finding of ocular surface tissues cytokeratins, for example, keratin 13 and keratin 12.Case Presentation. A 76-year-old man consulted us for visual disturbance with localized opacification of the corneal epithelium in his left eye. His visual acuity was 20/20 and 20/200 in his right and left eye, respectively. Slit lamp examination showed a whitish plaque-like lesion at the center of his left corneal epithelium. No vascular invasion to the lesion was found. The lesion was surgically removed and subjected to histopathological examination and diagnosed as epithelial dysplasia. Amyloidosis was excluded by direct fast scarlet 4BS (DFS) staining. Immunohistochemistry showed that the dysplastic epithelial cells express keratin 13 and vimentin, but not keratin 12, indicating that the neoplastic epithelial cells lacked corneal-type epithelium differentiation.Conclusions. The lesion was diagnosed as nonvascularized epithelial dysplasia of ocular surface. Etiology of the lesion is not known.

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mTOR-RAPTOR and 14-3-3 immunohistochemical expression in high grade prostatic intraepithelial neoplasia and prostatic adenocarcinomas: a tissue microarray study

Journal of Clinical Pathology ◽

10.1136/jclinpath-2011-200083 ◽

2011 ◽

Vol 64 (8) ◽

pp. 683-688 ◽

Cited By ~ 12

Author(s):

S. Evren ◽

A. Dermen ◽

G. Lockwood ◽

N. Fleshner ◽

J. Sweet

Keyword(s):

Tissue Microarray ◽

Intraepithelial Neoplasia ◽

Prostatic Intraepithelial Neoplasia ◽

High Grade ◽

Immunohistochemical Expression ◽

Microarray Study

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Association of Serum PSA Levels with Histopathological Pattern of Prostate Lesions

Journal of Islamabad Medical & Dental College ◽

10.35787/jimdc.v8i2.362 ◽

2019 ◽

Vol 8 (2) ◽

pp. 92-95 ◽

Cited By ~ 1

Author(s):

Afra Samad ◽

Nudrat Fayyaz ◽

Ayesha Siddiqa ◽

Naseem Akhter ◽

Rabia Saeed ◽

...

Keyword(s):

Benign Prostatic Hyperplasia ◽

Prostate Carcinoma ◽

Histopathological Examination ◽

Prostate Gland ◽

Specific Antigen ◽

Digital Rectal Examination ◽

Intraepithelial Neoplasia ◽

Prostatic Intraepithelial Neoplasia ◽

Prostatic Hyperplasia ◽

P Value

Background: Pathological changes that mainly affect prostate gland are prostatitis, benign prostatic hyperplasia (BPH) and cancerous lesions. Digital rectal examination (DRE), Transrectal Ultrasonography (TUS), and prostate specific antigen (PSA) followed by histopathological examination, are routinely used tests for diagnosis of prostate lesions. The aim of the present study is to determine the role of serum PSA levels in differentially diagnosing the different types of prostate lesions.Material and Methods: This retrospective (observational) study was conducted in Ibn-e-Sina Hospital Multan. Data of 2189 patients who were operated from 2007 to 2017 due to prostatic lesions were included in this analysis. Patients with BPH, prostatitis, prostate carcinoma and Prostatic Intraepithelial Neoplasia (PIN) were grouped according to serum PSA levels (ranging from 0 to >100 ng/ml) into five groups. Frequencies and percentages were calculated for different histopathological findings. Association of PSA levels with different histological patterns was determined with chi-square test with P-value < 0.05 taken as significant difference.Results: Mean age of patients was 62.45+10.64 years. On histopathology, BPH was diagnosed in 1676 (76.56%) patients, prostatitis in 133 (6.07%), carcinoma in 378 (17.26%) and PIN in 02 (0.09%) patients, respectively. Serum PSA levels of 4.01-10 ng/ml were found in 1050 (62.64%) BPH patients and in 59 (44.36%) prostatitis patients. Serum PSA levels of 10.01-20 ng/ml were found in only 40 (2.4%) BPH patients, 47 (35.33%) prostatitis patients, 22 (5.82%) carcinoma patients and in 1 (50.0%) PIN patient. Serum PSA levels of 20.01-100 ng/ml were found in 32 (1.9%) BPH patients, 11 (8.27%) prostatitis patients, 302 (79.89%) carcinoma patients, and in 1 (50.0%) PIN patient. Serum PSA levels of >100 ng/ml were absent in patients with BPH and PIN, and present in 1 (0.75%) prostatitis and 54 (14.28%) carcinoma patients.Conclusion: Benign prostatic hyperplasia was the commonest lesion in our patients (76.56%) with serum PSA levels >10 ng/ml reported in all patients with prostate carcinoma and prostatic intraepithelial neoplasia (PIN) patients.

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