scholarly journals Study the effect of some solvents on the λmax and absorbance of bromo phenol blue and phenol red indicators

2021 ◽  
pp. 894-901
Author(s):  
Hamad. M. Adress. Hasan ◽  
Huda S. Muftah ◽  
Nada. Hemdan. Ahmed
Keyword(s):  
Electronic Transitions ◽  
Bromophenol Blue ◽  
Phenol Red ◽  
Effect Of Solvents ◽  
Phenol Blue

This study which carried out on some indicators including (Phenol red and Bromo phenol Blue). Different solvents were used including (Methanol, propanol, DMF and DMSO). The effect of solvents on the λ max and absorbance values were investigated. The results recorded that the λmax values were effected and changed according to the polarities of the applied solvents. Also, the obtained results showed changes in the absorbance values after applied solvents compared with the original absorbance values. Keywords: Bromophenol blue; Phenol red; Effect of solvents on electronic transitions

scholarly journals Ce-MOF with Intrinsic Haloperoxidase-Like Activity for Ratiometric Colorimetric Detection of Hydrogen Peroxide

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 204
Author(s):  
Yanyan Cheng ◽  
Ling Liang ◽  
Fanggui Ye ◽  
Shulin Zhao
Keyword(s):  
Structural Characteristics ◽  
Metal Organic Framework ◽  
Colorimetric Detection ◽  
Mixed Valence ◽  
Bromophenol Blue ◽  
Phenol Red ◽  
Chromogenic Substrate ◽  
Colorimetric Sensing ◽  
Sensing Platform ◽  
Metal Organic

Metal–organic framework (MOF) nanozymes, as emerging members of the nanozymes, have received more and more attention due to their composition and structural characteristics. In this work, we report that mixed-valence state Ce-MOF (MVCM) has intrinsic haloperoxidase-mimicking activity. MVCM was synthesized by partial oxidation method using Ce-MOF as a precursor. In the presence of H2O2 and Br−, MVCM can catalyze oxidative bromination of chromogenic substrate phenol red (PR) to produce the blue product bromophenol blue (Br4PR), showing good haloperoxidase-like activity. Because of the special chromogenic substrate, we constructed a ratiometric colorimetric-sensing platform by detecting the absorbance of the MVCM-(PR, Br−) system at wavelengths of 590 and 430, for quantifying H2O2, where the detection limit of the H2O2 is 3.25 μM. In addition, the haloperoxidase-mimicking mechanism of the MVCM is proposed. Moreover, through enzyme kinetics monitoring, the Km (H2O2 and NH4Br) of the MVCM is lower than that of cerium oxide nanomaterials, indicating that the MVCM has a stronger binding affinity for H2O2 and NH4Br than other materials. This work provides more application prospects for the development of nanozymes in the field of biosensors in the future.

scholarly journals THE INFLUENCE OF INFLAMMATION ON THE ABSORPTION OF SUBSTANCES OF VARIED DIFFUSIBILITY

1938 ◽  
Vol 67 (4) ◽  
pp. 619-641 ◽  
Author(s):  
Rose G. Miller
Keyword(s):  
Peritoneal Cavity ◽  
Subcutaneous Tissue ◽  
Horse Serum ◽  
Type I ◽  
Phenol Red ◽  
Serum Globulin ◽  
Egg Albumin ◽  
Specific Polysaccharide ◽  
Phenol Blue ◽  
Pneumococcus Type

1. Inflammation retards the absorption of horse serum globulin and crystalline egg albumin from the peritoneal cavity and subcutaneous tissue, but retardation of the absorption of crystalline egg albumin is less than that of globulin, which is less diffusible. 2. Inflammation retards the absorption of the specific polysaccharide of pneumococcus Type I from the peritoneal cavity; inflammation may accelerate, but does not hinder, the absorption of glucose from the peritoneal cavity. 3. Inflammation retards the spread of trypan blue in the skin, but accelerates absorption from the skin of the more diffusible dye, brom phenol blue. 4. Phenol red is excreted in the urine with equal rapidity after injection into normal and into inflamed subcutaneous tissue or into normal and into inflamed peritoneal cavities. Direct extractions of phenol red from inflamed subcutaneous sites indicate that inflammation accelerates the absorption of the dye from these areas. 5. Inflammation retards the absorption of the indiffusible proteins, carbohydrates and dyes; it tends to accelerate the absorption of the diffusible carbohydrates and dyes.

scholarly journals Effect Of Metal Ions On Triphenylmethane Dye Decolorization By Laccase From Trametes Versicolor

2015 ◽  
Vol 14 (2) ◽  
pp. 191-200 ◽  
Author(s):  
Daniela Chmelová ◽  
Miroslav Ondrejovič
Keyword(s):  
Metal Ions ◽  
Trametes Versicolor ◽  
Laccase Activity ◽  
Dye Decolorization ◽  
Redox Mediator ◽  
White Rot ◽  
Bromophenol Blue ◽  
Phenol Red ◽  
Triphenylmethane Dye ◽  
Monovalent Ions

Abstract The aim of this study was investigate the influence of different metal ions on laccase activity and triphenylmethane dye decolorization by laccase from white-rot fungus Trametes versicolor. Laccase activity was inhibited by monovalent ions (Li+, Na+, K+ and Ag+) but the presence of divalent ions increased laccase activity at the concentration of 10 mmol/l. The effect of metal ions on decolorization of triphenylmethane dyes with different structures namely Bromochlorophenol Blue, Bromophenol Blue, Bromocresol Blue and Phenol Red was tested. The presence of metal ions (Na+, K+, Mg2+, Ca2+, Ba2+, Mn2+, Zn2+) slightly decreased triphenylmethane dye decolorization by laccase from T. versicolor except Na+ and Mg2+, which caused the increase of decolorization for all tested dyes. Decolorization of selected dyes showed that the presence of low-molecular-weight compounds is necessary for effective decolorization. Hydroxybenzotriazole (HBT) is the most frequently used. Although HBT belongs to most frequently used redox mediator and generally increase decolorization efficiency, so its presence decreased decolorization percentage of Bromophenol Blue and Bromochlorophenol Blue, the influence of metal ions to dye decolorization by laccase has the similar course with or without presence of redox mediator HBT.

scholarly journals Simple and extraction-free spectrophotometric methods for risperidone in pure form and in dosage forms

2013 ◽  
Vol 19 (1) ◽  
pp. 121-128 ◽  
Author(s):  
Hemavathi Deepakumari ◽  
Shiramahally Mallegowda ◽  
Kanakapura Vinay ◽  
Hosakere Revanasiddappa
Keyword(s):  
Limit Of Detection ◽  
Ion Pairs ◽  
Pharmaceutical Preparations ◽  
Reference Method ◽  
Standard Addition ◽  
Bromophenol Blue ◽  
Phenol Red ◽  
Spectrophotometric Methods ◽  
Accuracy And Precision

Two simple, sensitive and extraction-free spectrophotometric methods are described for the estimation of risperidone (RSP) in both pure and in pharmaceutical preparations. The proposed methods are based on the formation of ion-pair complex between RSP and the dyes, bromophenol blue (BPB) in method A and Phenol red (PR) in method B at room temperature to form yellow colored products which show maximum absorbance at 410 and at 400 nm in methods A and B, respectively. Beer's law was obeyed in the concentration range of 0.5-10 and 0.5-25 ?g mL-1 in methods A and B with apparent molar absorptivities of 3.43 ? 104 and 0.85 ? 104 L moL-1 cm-1, respectively. The limit of detection for method A is found to be 0.0056 and for method B is 0.132 ?g mL-1. The composition of the ion-pairs was established by Job?s method and it was found to be 1:1 for both the methods A and B. The proposed methods have been applied successfully to the determination of RSP in pharmaceutical preparations. The results were statistically compared with those of a reference method by applying the Student?s t-test and F-test. The methods developed were validated for accuracy and precision by performing recovery experiments via standard addition technique.

scholarly journals Dye inhibition of phosphate transport in everted duodenal sacs of mice

2020 ◽  
Vol 1 (1) ◽  
pp. 18-20
Author(s):  
Panthalookaren Lonappan Mary ◽  
Jonnakuty Prakasa Rao ◽  
Ojashwi Nepal
Keyword(s):  
Kidney Function ◽  
Phosphate Transport ◽  
Bromocresol Green ◽  
Bromophenol Blue ◽  
Bathing Solution ◽  
Primary Process ◽  
Phenol Red ◽  
Everted Gut Sac ◽  
Mouse Intestine ◽  
Uptake And Release

Phenol Red has been widely used to test kidney function in man. Using the simple, everted gut sac technique has been observed to inhibit the phosphate transport by phenol red in the mouse intestine. We wanted to see if other similar organic anions are able to inhibit the phosphate transport across the mouse intestine. Both uptake and release of phosphate by the everted duodenal sacs of mice are inhibited by phenol red, bromocresol green and bromophenol blue. At the highest dose all the dyes were able to inhibit both influx and efflux significantly. Loss of phosphate from bathing solution is taken as influx and the gain of phosphate by the solution within the sac is taken efflux. At higher dosages a trend of increase in E/I% was noted. At the highest dose all the dyes were able to increase this parameter significantly over the control. Influx appears to be the primary process to be affected. Possible use of phenol red, on account of its safety in humans, as a hypophosphatemic agent is suggested.

3d-4s and 3d-4p electronic transitions in M2+ : NaF AND M2+ : KMgF3 (Μ = V, Cr, and Mn). Results of a cluster-model calculation

1987 ◽  
Vol 84 ◽  
pp. 855-861 ◽  
Author(s):  
M. Flórez ◽  
M. Bermejo ◽  
V. Luaña ◽  
E. Francisco ◽  
J.M. Recio ◽  
...  
Keyword(s):  
Model Calculation ◽  
Cluster Model ◽  
Electronic Transitions

Effect of fumarprotocetraric acid isolated the Lichen Cladonia verticillaris on tracheobronchial phenol red excretion in mice

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
GP Duarte ◽  
GD Alves ◽  
ED Franco ◽  
RG Melo ◽  
DP Cordeiro ◽  
...  
Keyword(s):  
Phenol Red
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