scholarly journals Effect Of Metal Ions On Triphenylmethane Dye Decolorization By Laccase From Trametes Versicolor

2015 ◽  
Vol 14 (2) ◽  
pp. 191-200 ◽  
Author(s):  
Daniela Chmelová ◽  
Miroslav Ondrejovič
Keyword(s):  
Metal Ions ◽  
Trametes Versicolor ◽  
Laccase Activity ◽  
Dye Decolorization ◽  
Redox Mediator ◽  
White Rot ◽  
Bromophenol Blue ◽  
Phenol Red ◽  
Triphenylmethane Dye ◽  
Monovalent Ions

Abstract The aim of this study was investigate the influence of different metal ions on laccase activity and triphenylmethane dye decolorization by laccase from white-rot fungus Trametes versicolor. Laccase activity was inhibited by monovalent ions (Li+, Na+, K+ and Ag+) but the presence of divalent ions increased laccase activity at the concentration of 10 mmol/l. The effect of metal ions on decolorization of triphenylmethane dyes with different structures namely Bromochlorophenol Blue, Bromophenol Blue, Bromocresol Blue and Phenol Red was tested. The presence of metal ions (Na+, K+, Mg2+, Ca2+, Ba2+, Mn2+, Zn2+) slightly decreased triphenylmethane dye decolorization by laccase from T. versicolor except Na+ and Mg2+, which caused the increase of decolorization for all tested dyes. Decolorization of selected dyes showed that the presence of low-molecular-weight compounds is necessary for effective decolorization. Hydroxybenzotriazole (HBT) is the most frequently used. Although HBT belongs to most frequently used redox mediator and generally increase decolorization efficiency, so its presence decreased decolorization percentage of Bromophenol Blue and Bromochlorophenol Blue, the influence of metal ions to dye decolorization by laccase has the similar course with or without presence of redox mediator HBT.

scholarly journals Decolorization of Phenol Red Dye by Immobilized Laccase in Chitosan Beads Using Laccase - Mediator - System Model

2020 ◽  
Vol 17 (3) ◽  
pp. 0720
Author(s):  
Ali A. Taha ◽  
Nahida J. Hameed ◽  
Farah H. Rashid
Keyword(s):  
Dye Decolorization ◽  
White Rot ◽  
White Rot Fungus ◽  
Phenol Red ◽  
Chitosan Beads ◽  
Immobilized Laccase ◽  
Enzyme Recycling ◽  
Mediator System ◽  
Red Dye

This work describes the enhancement of phenol red decolorization through immobilizing of laccase in chitosan and enzyme recycling. Commercial laccase from white rot fungus, Trametesversicolor (Tvlac), was immobilizedin to freshly prepared chitosan beads by using glutaraldehyde as a cross linker. Characterization of prepared chitosan was confirmed by FTIR and scanning electron microscope (SEM). Tvlac (46.2 U/mL) immobilized into chitosan beads at 0.8 % glutaraldehyde (v/v) within 24 hrs. Synthetic (HBT) and natural (vanillin) mediators were used to enhance dye decolorizoation. It was found that 89 % of phenol red was decolorized by chitosan beads within 180 min. in the absence of enzyme and mediator, while decolorization percentage of the dye was completed (100%) at 120 min. when chitosan immobilizedlaccase was applied. Moreover, the decolorization was completed within 25 and 50 min. in the presence of chitosan immobilized laccase and of HBT or vanillin respectively. On the other hand, the recycling of chitosan immobilized laccase was still decolorize phenol red and continued up to ninth cycle to reach  70% of dye decolorization .

Enhanced laccase activity of white rot fungi induced by different metal ions under submerged fermentation

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 8369-8383
Author(s):  
Qi An ◽  
Mei-Ling Han ◽  
Lu-Sen Bian ◽  
Zhi-Chao Han ◽  
Ning Han ◽  
...  
Keyword(s):  
Metal Ions ◽  
Metal Ion ◽  
Laccase Activity ◽  
Copper Ions ◽  
Copper Ion ◽  
White Rot Fungi ◽  
White Rot ◽  
Ferrous Ions ◽  
Mixed Metal ◽  
Manganese Ion

Submerged fermentation with single or mixed metal ions as inducers was used for laccase production from white rot fungi. Mixed metal ions were used for the first time as inducers for Pleurotus ostreatus and Flammulina velutipes to enhance laccase activity. The maximum laccase activity from P. ostreatus in basal media, metal ion media 1 containing copper ion, metal ion media 2 containing manganese ion, metal ion media 3 containing manganese and copper ions, metal ion media 4 containing ferrous ion, metal ion media 5 containing manganese and ferrous ions, metal ion media 6 containing ferrous and copper ions, and metal ion media 7 containing manganese, copper and ferrous ions were, respectively, approximately 21.5-fold, 4.7-fold, 14.9-fold, 16.9-fold, 4.0-fold, 11.0-fold, 12.7-fold, and 24.8-fold higher than that from F. velutipes. The combination of copper and manganese ions as inducers was superior to that of a single copper ion or manganese ion. The maximum laccase activity of P. ostreatus rose in media containing manganese and copper ions. The single copper ion as the inducer for enhancing laccase activity was more suitable for F. velutipes. These findings are helpful in selecting the appropriate single metal ion or mixed metal ions to enhance laccase activity.

Transformation of Chlorophenols by White-Rot Fungi and their Laccase

Holzforschung ◽  
2001 ◽  
Vol 55 (6) ◽  
pp. 579-584 ◽  
Author(s):  
N.-S. Cho ◽  
J.-H. Nam ◽  
J.-M. Park ◽  
C.-D. Koo ◽  
S.-S. Lee ◽  
...  
Keyword(s):  
Trametes Versicolor ◽  
Laccase Activity ◽  
Fungal Growth ◽  
White Rot Fungi ◽  
Control Culture ◽  
White Rot ◽  
Cerrena Unicolor

SummaryThe effects of pentachlorophenol (PCP) and 2-chlorophenol (2CP) on white rot fungi,Abortiporus biennis, Cerrena unicolor, Gloeophyllum odoratum, andTrametes versicolorwere investigated. With the exception ofC. unicolorandA. biennis, concentrations of PCP higher than 50 ppm inhibited fungal growth completely. However, extreme concentrations of PCP (500 ppm) caused a 61% increase in growth ofC. unicolorcompared to the control culture within 14 days. When initial PCP concentrations were 50 ppmA. biennisandC. unicolorachieved, respectively, 87% and 78% of chlorophenol transformation within 3 days. However, when 2,5-dimethylaniline (0.2mM) was added to theC. unicolorculture, 98% of PCP transformation was achieved after one hour. Under the same conditions,A. biennistransformed 44% of PCP. Both chlorophenols are completely removed, when laccase activity reaches its maximum. In conclusion, among the four fungi investigated,C. unicolorandA. Bienniswere very effective in transforming PCP and 2-CP. This biotransformation was greatly intensified by 2,5-dimethylaniline and accompanied by an increase in laccase activity.

scholarly journals Role of Laccase and Low Molecular Weight Metabolites fromTrametes versicolorin Dye Decolorization

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Diego Moldes ◽  
María Fernández-Fernández ◽  
M. Ángeles Sanromán
Keyword(s):  
Molecular Weight ◽  
Reaction Mechanism ◽  
Crystal Violet ◽  
Trametes Versicolor ◽  
Dye Decolorization ◽  
Low Molecular Weight ◽  
Phenol Red ◽  
Decolorization Of Dyes

The studies regarding decolorization of dyes by laccase may not only inform about the possible application of this enzyme for environmental purposes, but also may provide important information about its reaction mechanism and the influence of several factors that could be involved. In this paper, decolorization of crystal violet and phenol red was carried out with different fractions of extracellular liquids fromTrametes versicolorcultures, in order to describe the role of laccase in this reaction. Moreover, the possible role of the low molecular weight metabolites (LMWMs) also produced by the fungus was evaluated. The results confirm the existence of a nonenzymatic decolorization factor, since the nonprotein fraction of the extracellular liquids from cultures ofT. versicolorhas shown decolorization capability. Several experiments were performed in order to identify the main compounds related to this ability, which are probably low molecular weight peroxide compounds.

scholarly journals Kinetic Evaluation of Dye Decolorization by Fenton Processes in the Presence of 3-Hydroxyanthranilic Acid

2019 ◽  
Vol 16 (9) ◽  
pp. 1602 ◽  
Author(s):  
Cássia Sidney Santana ◽  
Márcio Daniel Nicodemos Ramos ◽  
Camila Cristina Vieira Velloso ◽  
André Aguiar
Keyword(s):  
Dye Degradation ◽  
Dye Decolorization ◽  
Redox Mediator ◽  
Phenol Red ◽  
Order Kinetic ◽  
Kinetic Evaluation ◽  
Chromotrope 2R ◽  
Different Temperatures ◽  
Oxidative Processes ◽  
Hydroxyanthranilic Acid

The fungal metabolite 3-hydroxyanthranilic acid (3-HAA) was used as a redox mediator with the aim of increasing dye degradation by Fenton oxidative processes (Fe2+/H2O2, Fe3+/H2O2). Its Fe3+-reducing activity can enhance the generation of reactive oxygen species as HO● radicals. Initially, the influence of 3-HAA on decolorization kinetics of five dyes (methylene blue, chromotrope 2R, methyl orange, phenol red, and safranin T) was investigated using decolorization data from a previous work conducted by the present research group. Fe3+-containing reaction data were well fitted with first-order and mainly second-order kinetic models, whereas the BMG (Behnajady, Modirshahla and Ghanbary) model obtained optimal fit to Fe2+. Improvements in kinetic parameters (i.e., apparent rate constants and maximum oxidation capacity) were observed with the addition of 3-HAA. In another set of experiments, a decrease in apparent activation energy was observed due to introducing 3-HAA into reactions containing either Fe2+ or Fe3+ in order to decolorize phenol red at different temperatures. This indicates that the redox mediator decreases the energy barrier so as to allow reactions to occur. Thus, based on recent experiments and the reaction kinetics models evaluated herein, pro-oxidant properties have been observed for 3-HAA in Fenton processes.

scholarly journals Cerrena unicolor Laccases, Genes Expression and Regulation of Activity

Biomolecules ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 468
Author(s):  
Anna Pawlik ◽  
Beata Ciołek ◽  
Justyna Sulej ◽  
Andrzej Mazur ◽  
Przemysław Grela ◽  
...  
Keyword(s):  
Laccase Activity ◽  
Environmental Changes ◽  
Specific Activity ◽  
Electrochemical Analysis ◽  
White Rot ◽  
White Rot Fungus ◽  
Cerrena Unicolor ◽  
Genes Expression ◽  
Regulation Of Activity ◽  
Laccase Genes

A white rot fungus Cerrena unicolor has been identified as an important source of laccase, unfortunately regulation of this enzyme genes expression is poorly understood. Using 1D and 2D PAGE and LC-MS/MS, laccase isoenzymes were investigated in the liquid filtrate of C. unicolor culture. The level of expression of laccase genes was measured using qPCR. The elevated concentrations of copper and manganese in the medium caused greatest change in genes expression and three laccase transcripts were significantly affected after culture temperature was decreased from 28 to 4 °C or increased to 40 °C. The small differences in the PAGE band intensities of individual laccase proteins were also observed, indicating that given compound affect particular laccase’s transcript. Analyses of laccase-specific activity, at all tested conditions, showed the increased activities as compared to the control, suggesting that enzyme is regulated at the post-translational stage. We observed that the aspartic protease purified from C. unicolor, significantly stimulate laccase activity. Moreover, electrochemical analysis of protease-treated laccase sample had 5 times higher redox peaks. The obtained results indicate that laccases released by C. unicolor are regulated at transcriptional, translational, and at the post-translational steps of gene expression helping fungus adapt to the environmental changes.

scholarly journals Ce-MOF with Intrinsic Haloperoxidase-Like Activity for Ratiometric Colorimetric Detection of Hydrogen Peroxide

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 204
Author(s):  
Yanyan Cheng ◽  
Ling Liang ◽  
Fanggui Ye ◽  
Shulin Zhao
Keyword(s):  
Structural Characteristics ◽  
Metal Organic Framework ◽  
Colorimetric Detection ◽  
Mixed Valence ◽  
Bromophenol Blue ◽  
Phenol Red ◽  
Chromogenic Substrate ◽  
Colorimetric Sensing ◽  
Sensing Platform ◽  
Metal Organic

Metal–organic framework (MOF) nanozymes, as emerging members of the nanozymes, have received more and more attention due to their composition and structural characteristics. In this work, we report that mixed-valence state Ce-MOF (MVCM) has intrinsic haloperoxidase-mimicking activity. MVCM was synthesized by partial oxidation method using Ce-MOF as a precursor. In the presence of H2O2 and Br−, MVCM can catalyze oxidative bromination of chromogenic substrate phenol red (PR) to produce the blue product bromophenol blue (Br4PR), showing good haloperoxidase-like activity. Because of the special chromogenic substrate, we constructed a ratiometric colorimetric-sensing platform by detecting the absorbance of the MVCM-(PR, Br−) system at wavelengths of 590 and 430, for quantifying H2O2, where the detection limit of the H2O2 is 3.25 μM. In addition, the haloperoxidase-mimicking mechanism of the MVCM is proposed. Moreover, through enzyme kinetics monitoring, the Km (H2O2 and NH4Br) of the MVCM is lower than that of cerium oxide nanomaterials, indicating that the MVCM has a stronger binding affinity for H2O2 and NH4Br than other materials. This work provides more application prospects for the development of nanozymes in the field of biosensors in the future.

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