scholarly journals A Study of the Effect of Bioagent Trichoderma harzianum Rifai, the Fungicide Topsin-M and their Interaction on Root Rot Disease of Okra Abelmoschus esculentus in the Field

2019 ◽  
Vol 32 ◽  
pp. 320-336
Author(s):  
Yehya A. Salih ◽  
Noor M. Mansoor
Keyword(s):  
Trichoderma Harzianum ◽  
Root Rot ◽  
Pathogenic Fungus ◽  
Pathogenic Fungi ◽  
Dry Weight ◽  
Macrophomina Phaseolina ◽  
Root Rot Disease ◽  
Fruit Productivity ◽  
Rot Disease ◽  
Fungus Growth

This study aimed to investigate the effect of interaction between Trichoderma harzianum and the fungicide Topsin-M on root rot disease that infected okra in the field. Three fungi were isolated from the root of okra that infected with root rot disease: Fusarium solani, Rhizoctonia solani and Macrophomina phaseolina. The pathogenicity of these fungi was tested and found to be they cause root rot disease on okra, the disease severity was 41.7, 6.7 and 31.7% respectively. The laboratorial experiments showed that T. harzianum had a high antagonism ability with degrees of 1 and 2 against the pathogenic fungi M. phaseolina, F. solani and R. solani respectively. Also, it was found that the fungicide Topsin –M inhibited the growth of all pathogenic fungi with a percent of 100%, while it inhibited the bioagent fungus growth with a percent of 50.4 %, therefore it be recommended for the interaction experiments. The field results showed that using of bioagent T. harzianum and fungicide topsin-M significantly reduced the infection percentage and severity disease of the pathogenic fungi F. solani, R. solani and M. phaseolina to 65.3, 21.20, 13.20, 46.20, 25.70 and 18.20% respectively, compared to each pathogenic fungus alone which were 71.00, 60.20, 60.20, 66.80, 80.20 and 60.20% respectively. The interaction between the bioagent T. harzianum and topsin-M led to increase the plant height, fresh and dry weight of shoot and root systems and the fruit productivity of the examined okra plants .

scholarly journals Local Trichoderma strains as a control strategy of complex black root rot disease of strawberry in Egypt

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Farid Abd-El-Kareem ◽  
Ibrahim E. Elshahawy ◽  
Mahfouz M. M. Abd-Elgawad
Keyword(s):  
Root Rot ◽  
Disease Incidence ◽  
Pathogenic Fungi ◽  
Dry Weight ◽  
Biological Pest Control ◽  
Potato Dextrose Broth ◽  
Black Root Rot ◽  
Root Rot Disease ◽  
Growth Area ◽  
Rot Disease

Abstract Background Economics and human safety to avoid health risks caused by fungicides are materializing new era of biological pest control. Trichoderma species ranked high among other agents to control complex black root rot disease of strawberry caused by Fusarium solani, Rhizoctonia solani, and Pythium sp. Our study aimed to document the efficacy of local strains representing T. harzianum, T. viride, T. virinis, and T. koningii against such a disease. Materials/methods These strains were cultured separately on potato dextrose broth medium to test their inhibitory effect against strawberry black root rot in vitro and in vivo. Strawberry growth and yield were also assessed relative to the untreated check and the fungicide Actamyl. Activity of peroxidase and chitinase were measured in plant leaves using spectrophotometer. Results Each of the antagonistic fungal strains significantly reduced growth area of all pathogenic fungi collectively causing the disease. Trichoderma harzianum, T. viride, and T. koningii reduced the growth area more than 90.6% for all tested pathogenic fungi. Each species significantly reduced disease incidence and severity under field conditions. The highest reduction in the disease incidence and severity, 83.3 and 88.5% respectively, was attained by mixture of the four species. This mixture increased the strawberry fresh and dry weight by 83.3 and 176.9%, respectively, and the yield by 117.1%. All Trichoderma species tested significantly increased the activity of two plant defense-related enzymes of strawberry plants against the pathogens. Their mixture attained the highest increase of peroxidase and chitinase activity by 150 and 160.9%, respectively. Conclusions While the fungal mixture could considerably increase the strawberry fresh and dry weight as well as the yield, it suppressed the incidence and severity of the disease. So, integrated pest management in ways that make these biocontrol agents complementary or superior to chemical fungicides should further be examined against this disease.

scholarly journals The Effect of Biochars and Endophytic Bacteria on Growth and Root Rot Disease Incidence of Fusarium Infested Narrow-Leafed Lupin (Lupinus angustifolius L.)

2020 ◽  
Vol 8 (4) ◽  
pp. 496
Author(s):  
Dilfuza Egamberdieva ◽  
Vyacheslav Shurigin ◽  
Burak Alaylar ◽  
Hua Ma ◽  
Marina E. H. Müller ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Rot ◽  
Endophytic Bacteria ◽  
Growth Parameters ◽  
Disease Incidence ◽  
Dry Weight ◽  
Microbial Interactions ◽  
Root Rot Disease ◽  
Root Dry Weight ◽  
Rot Disease

The effects of biochar on plant growth vary depending on the applied biochar type, study site environmental conditions, microbial species, and plant–microbial interactions. The objectives of the present study were therefore to assess 1) the response of growth parameters of lupin and root disease incidence to the application of three biochar types in a loamy sandy soil, and 2) the role of endophytic bacteria in biological control of root rot disease incidence in lupin after the amendment of soil with different biochar types. As biochar types we tested (i) hydrochar (HTC) from maize silage, (ii) pyrolysis char from maize (MBC), and (iii) pyrolysis char from wood (WBC) at three different concentrations (1%, 2%, and 3% of char as soil amendments). There were no significant effects in lupin shoot and root growth in soils amended with WBC at any of the concentrations. MBC did not affect plant growth except for root dry weight at 2% MBC. HTC char at 2% concentration, significantly increased the root dry weight of lupin by 54–75%, and shoot dry weight by 21–25%. Lupin plants grown in soil amended with 2% and 3% WBC and MBC chars showed 40–50% and 10–20% disease symptoms, respectively. Plants grown in soil without biochar and with HTC char were healthy, and no disease incidence occurred. Pseudomonas putida L2 and Stenotrophomonas pavanii L8 isolates demonstrated a disease reduction compared to un-inoculated plants under MBC and WBC amended soil that was infested with Fusarium solani.

scholarly journals Role of Bradyrhizobium japonicum and Trichoderma spp. in the control of root rot disease of soybean

2014 ◽  
Vol 30 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Syed Ehteshamul-Haque ◽  
Abdul Ghaffar
Keyword(s):  
Grain Yield ◽  
Rhizoctonia Solani ◽  
Trichoderma Harzianum ◽  
Root Rot ◽  
Bradyrhizobium Japonicum ◽  
Seed Treatment ◽  
Trichoderma Spp ◽  
Root Rot Disease ◽  
Rot Disease

Seed treatment of soybean with <i>Bndyrhizobium japonicum, Trichoderma harzianum, T. viride, T. hamatum, T. koningii</i> and <i>T. pseudokoningii</i> significantly controlled the infection of 30-day-old seedlingsby <i>Maerophomina phaseolina, Rhizoctonia solani</i> and <i>Fusarium</i> spp. In 60-day-old plants <i>Trichoderma</i> spp.. and <i>B. japonicum</i> inhibited the grouth of <i>R. solani</i> and <i>Fusarium</i> spp., whereas the use of <i>B. japonicum</i> (TAL-102) with <i>T. harzianum. T. viride, T. koningii</i> and <i>T. pseudokoningii</i> controlled the infection by <i>M. phaseolina. Greater grain yield was recorded when B. <i>japonium</i> (TAI-102) was used with <i>T. hamatum</i>.

Screening of wild Solanum rootstock to manage dry root rot disease (Macrophomina phaseolina) in brinjal

1970 ◽  
pp. 34
Author(s):  
J. Sherly, R. Renuka
Keyword(s):  
Root Rot ◽  
Disease Incidence ◽  
Macrophomina Phaseolina ◽  
Solanum Species ◽  
Wild Solanum Species ◽  
Root Rot Disease ◽  
House Condition ◽  
Rot Disease ◽  
Cent Disease ◽  
Wild Solanum

Five wild Solanum species were screened against dry root rot disease in glass house condition with commonly cultivated brinjal as control. The disease was scored at different day’s interval on 30th, 45th and 60th days after planting (DAP). Among the wild species screened and evaluated the species S. torvum was observed without any disease incidence i.e (0). The least per cent of disease incidence was observed in S. incanum (38.60%) which was followed by S. xanthocarpum (42.80%), S. viarum (64.20%) and CO2 (78.50 %) susceptible check at 30 DAP. Secondary metabolites were analysed from both wild and control plants. Based on the per cent disease incidence values S. torvum was graded as ‘Resistant’ against M. phaseolina and can be recommended as a best rootstock for brinjal under varying climatic condition especially where the dry climate prevails.

scholarly journals Evaluation of plant extracts, fungicides, bio-agent and soil amendments against foot and root rot disease of betelvine caused by Sclerotium rolfsii under field condition

2020 ◽  
Vol 44 (4) ◽  
pp. 669-677
Author(s):  
MH Rahman ◽  
MR Islam ◽  
FM Aminuzzaman ◽  
A Latif ◽  
S Nahar
Keyword(s):  
Trichoderma Harzianum ◽  
Root Rot ◽  
Untreated Control ◽  
Disease Incidence ◽  
Sclerotium Rolfsii ◽  
Control Treatment ◽  
Piper Betle ◽  
Root Rot Disease ◽  
Clove Extract ◽  
Rot Disease

The studies were carried out on the management of foot and root rot disease caused by Sclerotium rolfsii Sacc. of betelvine (Piper betle L.) during April, 2017 to April, 2018 in a betelvine orchard (baroj) in the experimental farm of Sher-e-Bangla Agricultural University, Dhaka. There were 10 treatments, namely Provax 200, Tilt 250 EC, Score 250 EC, Pencozeb 80 WP, Garlic clove extract, Allamanda leaf extract, Poultry waste, Vermi-compost, Trichoderma harzianum and Control. The in-vivo evatuation, plant was inoculated by S. rolfsii after six month of plantation. The evaluation revealed that the lowest disease incidence of 8.33% was found in Provax 200 whereas the highest disease incidence of 91.67 was recorded under the untreated control treatment. The maximum of 90.91% reduction in disease incidence over control was found under Provax 200, which was significantly higher compared to all other treatments. Among the treatments, Provax 200 was noted as the most effective fungicide followed by Score 250 EC. The highest yield (7.57 t/ha) at 120 days after inoculation was found in case of Provax 200 which was 330.1% increased over untreated control. Stem and soil treated with Provax 200 including spraying at 3 days after inoculation, then sprayed at 7 days’ intervals minimized disease incidence and increased yield. Among the eco-friendly approach, Garlic clove extract, Trichoderma harzianum and soil amended with Vermi-compost also showed better performance in controlling foot and root rot disease of betelvine as compared to control. Bangladesh J. Agril. Res. 44(4): 669-677, December 2019

scholarly journals Effectiveness of different substrate materials to prepare Trichoderma harzianum based bio-fungicides to control foot and root rot (Fusarium oxysporum) of tomato

2015 ◽  
Vol 40 (2) ◽  
pp. 279-289 ◽  
Author(s):  
MI Faruk ◽  
ML Rahman ◽  
MM Rahman ◽  
R Islam ◽  
MA Rahman
Keyword(s):  
Fusarium Oxysporum ◽  
Rice Bran ◽  
Wheat Bran ◽  
Trichoderma Harzianum ◽  
Root Rot ◽  
Root Weight ◽  
Grass Pea ◽  
Tomato Seedling ◽  
Root Rot Disease ◽  
Rot Disease

An investigation was undertaken to evaluate the effectiveness Trichoderma harzianum based bio-fungicides multiplied on different substrates. The substrates was rice bran, wheat bran, grass pea bran and their combinations with mustard oilcake (MOC) were used to mass culture T. harzianum for the management of foot and root rot disease of tomato seedling caused by Fusarium oxysporum in seedbed. All combinations of carrier materials were found effective for preparing T. harzianum based bio-fungicides to promote germination, seedling growth and reducing pre-emergence and post-emergence mortality of tomato seedling under F. oxysporum inoculated seedbed soils. The shoot length, shoot weight, root length and root weight of tomato seedling were enhanced significantly by the application of different substrate materials of T. harzianum based bio-fungicides under F. oxysporum inoculated seedbed conditions. The individual (rice bran, wheat bran, grass pea bran) and combination of substrates (rice bran + wheat bran, rice bran + mustard oilcake, rice bran + wheat bran + MOC and wheat bran + grass pea bran + MOC) were equally suitable for mass culturing of effective T. harzianum bio-fungicides for the management of foot and root rot disease of tomato seedling in seedbed condition.Bangladesh J. Agril. Res. 40(2): 279-289 June 2015

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