Expression of Let-7c in invasive ductal carcinoma of breast cancer and its clinical significance

Aims and background Often neglected by large clinical trials, patients with uncommon breast malignancies have been rarely analyzed in large series. Patients and methods Of 2,052 patients diagnosed with breast cancer and followed in our Institution from January 1985 to December 2009, we retrospectively collected data on those with uncommon histotypes, with the aim of investigating their presentation characteristics and treatment outcome. Results Rare histotypes were identified in 146 patients (7.1% of our total breast cancer population), being classified as follows: tubular carcinoma in 75 (51.4%), mucinous carcinoma in 36 (24.7%), medullary carcinoma in 25 (17.1%) and papillary carcinoma in 10 patients (6.8%). Whereas age at diagnosis was not significantly different among the diverse diagnostic groups, patients with medullary and papillary subtypes had a higher rate of lymph node involvement, similar to that of invasive ductal carcinoma. Early stage diagnosis was frequent, except for medullary carcinoma. Overall, in comparison with our invasive ductal carcinoma patients, those with rare histotypes showed a significantly lower risk of recurrence, with a hazard ratio of 0.28 (95% CI, 0.12–0.62; P = 0.002). Conclusions According to our analysis, patients with uncommon breast malignancies are often diagnosed at an early stage, resulting in a good prognosis with standard treatment.

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StackBC: Deep learning and transfer learning techniques based stacking approach for accurate Invasive Ductal Carcinoma classification using histology images

Journal of Intelligent & Fuzzy Systems ◽

10.3233/jifs-212240 ◽

2022 ◽

pp. 1-12

Author(s):

Amin Ul Haq ◽

Jian Ping Li ◽

Samad Wali ◽

Sultan Ahmad ◽

Zafar Ali ◽

...

Keyword(s):

Breast Cancer ◽

Deep Learning ◽

Transfer Learning ◽

Invasive Ductal Carcinoma ◽

Ductal Carcinoma ◽

Early Recognition ◽

Predictive Performance ◽

Operating Characteristics ◽

Data Set ◽

Learning Techniques

Artificial intelligence (AI) based computer-aided diagnostic (CAD) systems can effectively diagnose critical disease. AI-based detection of breast cancer (BC) through images data is more efficient and accurate than professional radiologists. However, the existing AI-based BC diagnosis methods have complexity in low prediction accuracy and high computation time. Due to these reasons, medical professionals are not employing the current proposed techniques in E-Healthcare to effectively diagnose the BC. To diagnose the breast cancer effectively need to incorporate advanced AI techniques based methods in diagnosis process. In this work, we proposed a deep learning based diagnosis method (StackBC) to detect breast cancer in the early stage for effective treatment and recovery. In particular, we have incorporated deep learning models including Convolutional neural network (CNN), Long short term memory (LSTM), and Gated recurrent unit (GRU) for the classification of Invasive Ductal Carcinoma (IDC). Additionally, data augmentation and transfer learning techniques have been incorporated for data set balancing and for effective training the model. To further improve the predictive performance of model we used stacking technique. Among the three base classifiers (CNN, LSTM, GRU) the predictive performance of GRU are better as compared to individual model. The GRU is selected as a meta classifier to distinguish between Non-IDC and IDC breast images. The method Hold-Out has been incorporated and the data set is split into 90% and 10% for training and testing of the model, respectively. Model evaluation metrics have been computed for model performance evaluation. To analyze the efficacy of the model, we have used breast histology images data set. Our experimental results demonstrated that the proposed StackBC method achieved improved performance by gaining 99.02% accuracy and 100% area under the receiver operating characteristics curve (AUC-ROC) compared to state-of-the-art methods. Due to the high performance of the proposed method, we recommend it for early recognition of breast cancer in E-Healthcare.

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Impact of Pathological Characteristics on Local Relapse After Breast-Conserving Therapy: A Subgroup Analysis of the EORTC Boost Versus No Boost Trial

Journal of Clinical Oncology ◽

10.1200/jco.2008.21.5764 ◽

2009 ◽

Vol 27 (30) ◽

pp. 4939-4947 ◽

Cited By ~ 160

Author(s):

Heather A. Jones ◽

Ninja Antonini ◽

Augustinus A.M. Hart ◽

Johannes L. Peterse ◽

Jean-Claude Horiot ◽

...

Keyword(s):

Breast Cancer ◽

Invasive Ductal Carcinoma ◽

Ductal Carcinoma ◽

Breast Conserving Therapy ◽

Local Relapse ◽

High Grade ◽

Invasive Tumor ◽

Boost Dose ◽

Central Pathology ◽

Pathology Review

Purpose To investigate the long-term impact of pathologic characteristics and an extra boost dose of 16 Gy on local relapse, for stage I and II invasive breast cancer patients treated with breast conserving therapy (BCT). Patients and Methods In the European Organisation for Research and Treatment of Cancer boost versus no boost trial, after whole breast irradiation, patients with microscopically complete excision of invasive tumor, were randomly assigned to receive or not an extra boost dose of 16 Gy. For a subset of 1,616 patients central pathology review was performed. Results The 10-year cumulative risk of local breast cancer relapse as a first event was not significantly influenced if the margin was scored negative, close or positive for invasive tumor or ductal carcinoma in situ according to central pathology review (log-rank P = .45 and P = .57, respectively). In multivariate analysis, high-grade invasive ductal carcinoma was associated with an increased risk of local relapse (P = .026; hazard ratio [HR], 1.67), as was age younger than 50 years (P < .0001; HR, 2.38). The boost dose of 16 Gy significantly reduced the local relapse rate (P = .0006; HR, 0.47). For patients younger than 50 years old and in patients with high grade invasive ductal carcinoma, the boost dose reduced the local relapse from 19.4% to 11.4% (P = .0046; HR, 0.51) and from 18.9% to 8.6% (P = .01; HR, 0.42), respectively. Conclusion Young age and high-grade invasive ductal cancer were the most important risk factors for local relapse, while margin status had no significant influence. A boost dose of 16 Gy significantly reduced the negative effects of both young age and high-grade invasive cancer.

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925 Utilizing multiplex immunofluorescence to explore the epithelial-mesenchymal transition in breast, ovarian cancers

Journal for ImmunoTherapy of Cancer ◽

10.1136/jitc-2021-sitc2021.925 ◽

2021 ◽

Vol 9 (Suppl 3) ◽

pp. A970-A970

Author(s):

Danielle Fails ◽

Michael Spencer

Keyword(s):

Breast Cancer ◽

Invasive Ductal Carcinoma ◽

Poor Prognosis ◽

Epithelial Mesenchymal Transition ◽

Ductal Carcinoma ◽

Beta Catenin ◽

Mesenchymal Transition ◽

Cell Counts ◽

Grade Ii ◽

E Cadherin

BackgroundEpithelial-mesenchymal transition (EMT) is instrumental during embryonic development—assisting in extensive movement and differentiation of cells. However, during metastasis and tumorigenesis, this process is hijacked. The disruption of this developmental process, and subsequent acquisition of a mesenchymal phenotype, has been shown to increase therapeutic resistance and often leads to poor prognosis in breast cancer.1 Using bioinformatic resources and current clinical data, we designed a panel of biomarkers of value to specifically observe this epithelial/mesenchymal transition.MethodsHuman breast cancer FFPE tissue samples were stained with Bethyl Laboratories IHC-validated primary antibodies, followed by Bethyl HRP-conjugated secondary antibodies, and detected using Akoya Opal™ Polaris 7-color IHC kit fluorophores (Akoya Biosciences [NEL861001KT]). The panel consisted of beta-Catenin, E-Cadherin, Ki67, CD3e, PD-L1, and FOXP3. Antibody staining order was optimized using tissue microarray serial sections, three slides per target, and stained in either the first, third, or sixth position via heat-induced epitope retrieval (HIER) methods. Exposure time was maintained for all three slides/target and cell counts, signal intensity, background, and autofluorescence were analyzed. The final optimized order was then tested on the breast cancer microarray in seven-color mIF. Whole slide scans were generated using the Vectra Polaris® and analyses performed using InForm® and R® Studio.ResultsTwo integral EMT targets, E-Cadherin and beta-Catenin, were used to observe a key occurrence in this transition. Under tumorigenic circumstances, when released from the complex they form together (E-cadherin-B-catenin complex), Beta-catenin can induce EMT. This disjunction/activation of EMT can be seen in the invasive ductal carcinoma below (figure 1).The disorganized E-cadherin cells are in direct contrast to normal, non-cancerous cells in similar tissue. Total CD3e cell counts were down (2%), with 35% cells restricted to the stroma vs. the 1% seen intra-tumorally. Coupled with the elevated presence of Ki67 (10%), a level of rapid cancer growth and potential metastasis (Invasive Ductal Carcinoma Grade II) can be observed.Abstract 925 Figure 1Invasive ductal carcinoma, grade II stained with a 6-plex mIF panel designed to show the epithelial-mesenchymal transitionConclusionsThe presence of EMT in breast cancers is often indicative of a poor prognosis, so the need for reliable markers is imperative. E-Cadherin and beta-Catenin are both up-and-coming clinical targets that can serve to outline this transition within the tumor microenvironment. By utilizing these markers in mIF, closer spatial examination of proteins of interest can be achieved. The application of this mIF panel has the potential to provide invaluable insights into how tumor infiltrating lymphocytes behave in cancers exhibiting the hallmarks of EMT.AcknowledgementsWe would like to acknowledge Clemens Deurrschmid, PhD, Technical Applications Scientist Southeast/South Central, Akoya Biosciences for his assistance with image analysis.ReferencesHorne HN, Oh H, Sherman ME, et al. E-cadherin breast tumor expression, risk factors and survival: pooled analysis of 5,933 cases from 12 studies in the breast cancer association consortium. Sci Rep 2018;8:6574.

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The Utilization of Physics Parameter to Classify Histopathology Types of Invasive Ductal Carcinoma (IDC) and Invasive Lobular Carcinoma (ILC) by using K-Nearest Neighbourhood (KNN) Method

International Journal of Electrical and Computer Engineering (IJECE) ◽

10.11591/ijece.v8i4.pp2442-2450 ◽

2018 ◽

Vol 8 (4) ◽

pp. 2442

Author(s):

Anak Agung Ngurah Gunawan ◽

I Wayan Supardi ◽

S. Poniman ◽

Bagus G. Dharmawan

Keyword(s):

Breast Cancer ◽

Invasive Ductal Carcinoma ◽

Invasive Lobular Carcinoma ◽

Ductal Carcinoma ◽

Lobular Carcinoma ◽

Diagnose Breast Cancer ◽

Object Based ◽

Computer Aided ◽

The Mean ◽

Learning Data

<p>Medical imaging process has evolved since 1996 until now. The forming of Computer Aided Diagnostic (CAD) is very helpful to the radiologists to diagnose breast cancer. KNN method is a method to do classification toward the object based on the learning data which the range is nearest to the object. We analysed two types of cancers IDC dan ILC. 10 parameters were observed in 1-10 pixels distance in 145 IDC dan 7 ILC. We found that the Mean of Hm(yd,d) at 1-5 pixeis the only significant parameters that distingguish IDC and ILC. This parameter at 1-5 pixels should be applied in KNN method. This finding need to be tested in diffrerent areas before it will be applied in cancer diagnostic.</p>

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Isolated right adrenal metastasis from an invasive ductal breast carcinoma.

10.32512/jmr/2.2.2019/10.13 ◽

2019 ◽

pp. 10-13

Keyword(s):

Breast Cancer ◽

Breast Carcinoma ◽

Invasive Ductal Carcinoma ◽

Adrenal Glands ◽

Ductal Carcinoma ◽

Adrenal Metastasis ◽

Breast Carcinomas ◽

Invasive Ductal Breast Carcinoma ◽

Invasive Breast Carcinomas ◽

Histopathological Type

Invasive ductal carcinoma (IDC) is the most common histopathological type of breast cancer, accounting for up to 85% of all invasive breast carcinomas [1]. It spreads usually to the bone first. Solitary metastasis is commonly located in the lung, liver or brain [2]. Adrenal glands locations are extremely rare [3]. We report a case of isolated metachronous right adrenal metastasis, diagnosed four years after breast IDC management. The aim is to highlight clinical, diagnostic and therapeutic characteristics of this entity.

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Histological classification of invasive ductal carcinoma and the biological parameters in breast cancer

Breast Cancer ◽

10.1007/bf02967640 ◽

2003 ◽

Vol 10 (2) ◽

pp. 149-152 ◽

Cited By ~ 3

Author(s):

Shinichi Tsutsui ◽

Shinji Ohno ◽

Shigeru Murakami ◽

Akemi Kataoka ◽

Junko Kinoshita ◽

...

Keyword(s):

Breast Cancer ◽

Invasive Ductal Carcinoma ◽

Ductal Carcinoma ◽

Histological Classification ◽

Biological Parameters

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Breast Cancer, Chronic Lymphocytic Leukaemia and Mucormycosis

Scottish Medical Journal ◽

10.1177/003693308703200511 ◽

1987 ◽

Vol 32 (5) ◽

pp. 150-151 ◽

Cited By ~ 2

Author(s):

L. G. McAlpine ◽

D. J. Williams ◽

J. H. Dagg

Keyword(s):

Breast Cancer ◽

Chronic Lymphocytic Leukaemia ◽

Invasive Ductal Carcinoma ◽

Lymphocytic Leukaemia ◽

Ductal Carcinoma ◽

Breast Tumour ◽

Tumour Recurrence ◽

The Other ◽

Prolonged Survival ◽

Breast Invasive Ductal Carcinoma

Prolonged survival following diagnosis of lipid-rich carcinoma of breast is unusual1. We report on a patient in whom lipid-rich carcinoma of one breast, invasive ductal carcinoma of the other breast and chronic lymphocytic leukaemia were diagnosed simultaneously; she survived 14 years without breast tumour recurrence and died with atypical mucormycosis.

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