S100 calcium‐binding protein A9 from tumor‐associated macrophage enhances cancer stem cell‐like properties of hepatocellular carcinoma

Hepatocarcinogenesis is a highly complicated process that is promoted by a series of oncogenes. Our study aims to identify novel oncogenes promoting hepatocellular carcinoma (HCC) by bioinformatic analysis and experimental validation. Here, we reported that S100 calcium binding protein A10 (S100A10) was screened out as a potential novel oncogene in HCC by integrated analysis of OEP000321 dataset and the Cancer Genome Atlas (TCGA)-Liver-Cancer data. Furthermore, S100A10 was highly expressed in HCC samples and observably associated with patients’ overall survival (OS). Overexpression of S100A10 in Hep3B and Huh-7 increased the cell proliferation, whereas downregulation of S100A10 in SK-Hep-1 and HepG2 cells reduced the cell viability to almost stop growing. In vivo tumor growth assays showed that S100A10-overexpressing Hep3B cells had a larger tumor size than control. Moreover, S100A10 overexpression promoted Hep3B cells migration and invasion, and S100A10 knockdown inhibited SK-Hep-1 cells migration and invasion, in vitro. In conclusion, it is demonstrated that S100A10 is a novel oncogene in HCC, indicating a possible novel therapeutic strategy of HCC.

Download Full-text

Effects of miRNA-136 overexpression on S100 calcium binding protein A6 contents, proliferation and apoptosis in HCT116 cells

Academic Journal of Second Military Medical University ◽

10.3724/sp.j.1008.2012.00940 ◽

2013 ◽

Vol 32 (9) ◽

pp. 940-944

Author(s):

Zhong-hua HUO ◽

Jun HU ◽

Zhu-ling CHU ◽

Bo SONG ◽

Sheng LV ◽

...

Keyword(s):

Calcium Binding ◽

Binding Protein ◽

Calcium Binding Protein ◽

S100 Calcium Binding Protein ◽

Proliferation And Apoptosis ◽

Hct116 Cells

Download Full-text

Calcium‐binding protein 39 promotes hepatocellular carcinoma growth and metastasis by activating extracellular signal‐regulated kinase signaling pathway

Hepatology ◽

10.1002/hep.29312 ◽

2017 ◽

Vol 66 (5) ◽

pp. 1529-1545 ◽

Cited By ~ 21

Author(s):

Lingxi Jiang ◽

Qian Yan ◽

Shuo Fang ◽

Ming Liu ◽

Yan Li ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Signaling Pathway ◽

Calcium Binding ◽

Binding Protein ◽

Calcium Binding Protein ◽

Kinase Signaling ◽

Extracellular Signal Regulated Kinase ◽

Extracellular Signal ◽

Kinase Signaling Pathway

Download Full-text

Anterior Gradient Protein 3 and S100 Calcium-Binding Protein P Levels in Different Endometrial Epithelial Compartments May Play an Important Role in Recurrent Pregnancy Failure

International Journal of Molecular Sciences ◽

10.3390/ijms22083835 ◽

2021 ◽

Vol 22 (8) ◽

pp. 3835

Author(s):

Nicola Tempest ◽

Elizabeth Batchelor ◽

Christopher J. Hill ◽

Hannan Al-Lamee ◽

Josephine Drury ◽

...

Keyword(s):

Calcium Binding ◽

Binding Protein ◽

Embryo Implantation ◽

Calcium Signalling ◽

Premenopausal Women ◽

Subcellular Location ◽

Early Embryo ◽

Calcium Binding Protein ◽

Regulatory Function ◽

S100 Calcium Binding Protein

Recurrent implantation failure (RIF) and recurrent pregnancy loss (RPL) are distressing conditions without effective treatments. The luminal epithelium (LE) is integral in determining receptivity of the endometrium, whereas functionalis glands and stroma aid in nurturing early embryo development. Calcium signalling pathways are known to be of vital importance to embryo implantation and pregnancy establishment, and anterior gradient protein 3 (AGR3) and S100 calcium-binding protein P (S100P) are involved with these pathways. We initially examined 20 full-thickness endometrial biopsies from premenopausal women across the menstrual cycle to characterize levels of AGR3 protein in each endometrial sub-region at the cellular level. A further 53 endometrial pipelle biopsies collected in the window of implantation were subsequently assessed to determine differential endometrial AGR3 and S100P levels relevant to RIF (n = 13) and RPL (n = 10) in comparison with parous women (n = 30) using immunohistochemistry. Significantly higher AGR3 and S100P immunostaining was observed in ciliated cells of the LE of women with recurrent reproductive failure compared with parous women, suggesting aberrant subcellular location-associated pathophysiology for these conditions. The nuclear localisation of S100P may allow transcriptional regulatory function, which is necessary for implantation of a viable pregnancy. Further work is thus warranted to assess their utility as diagnostic/therapeutic targets.

Download Full-text