Methodology Research of High-speed Counter-current Chromatography in Separation of Natural Products

2009 ◽  
Vol 6 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Shun YAO
Keyword(s):  
Natural Products ◽  
High Speed ◽  
Counter Current

One-step Separation and Purification of Four Phenolic Acids from Stenoloma chusanum (L.) Ching by Medium-pressure Liquid Chromatography and High-speed Counter-current Chromatography

2019 ◽  
Vol 9 (2) ◽  
pp. 138-143
Author(s):  
Tianyun Li ◽  
Xiling Dai ◽  
Yichen Li ◽  
Guozheng Huang ◽  
Jianguo Cao
Keyword(s):  
Natural Products ◽  
Liquid Chromatography ◽  
Phenolic Acids ◽  
High Speed ◽  
High Efficiency ◽  
Total Phenolic ◽  
Medium Pressure ◽  
Medium Pressure Liquid Chromatography ◽  
One Step ◽  
Counter Current

Background:Stenoloma chusanum (L.) Ching is a Chinese traditional medicinal fern with high total flavonoid and total phenolic content. Traditionally, phenolic compounds were separated by using column chromatography, which is relatively inefficient. </P><P> Objective: This study aims to use an efficient method to separate natural products from S. chusanum by Medium-Pressure Liquid Chromatography (MPLC) and High-Speed Counter-Current Chromatography (HSCCC).Methods:In the present research, firstly, a sample (2.5 g) from the dichloromethane extract of S. chusanum was separated by MPLC. Next, fraction P5 was purified by HSCCC with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water (HEMWat) at a volume ratio of 2:4:1:4 (v/v/v/v). </P><P> Result: Four phenolic acids were obtained and their structures were identified by means of NMR and ESI-mass analysis. They were identified as: 1) protocatechuic acid (34 mg, purity 90.1%), 2) syringic acid (66 mg, purity 99.0%), 3) p-hydroxybenzoic acid (5 mg, purity 91.2%) and 4) vanillic acid (6 mg, purity 99.3%).Conclusion:The combination of MPLC and HSCCC is a high-efficiency separation method for natural products. This is the first report with regard to the separation of four phenolic acids in one step by MPLC and HSCCC from S. chusanum (L.) Ching.

scholarly journals Development of online-storage inner-recycling counter-current chromatography for the preparative separation of complex components of alkylphenols from sarcotesta of Ginkgo biloba L.

RSC Advances ◽  
2018 ◽  
Vol 8 (60) ◽  
pp. 34321-34330 ◽  
Author(s):  
Daijie Wang ◽  
Xiangyun Song ◽  
Huijiao Yan ◽  
Mengmeng Guo ◽  
Ruiming Fu ◽  
...  
Keyword(s):  
Natural Products ◽  
Ginkgo Biloba ◽  
High Speed ◽  
Separation Method ◽  
Preparative Separation ◽  
Online Storage ◽  
Counter Current ◽  
Complex Components

High-speed counter-current chromatography (HSCCC) is becoming an effective and non-absorptive separation method from natural products.

Applications of high-speed counter-current chromatography for the separation and isolation of natural products

1991 ◽  
Vol 538 (1) ◽  
pp. 193-202 ◽  
Author(s):  
Norbert Fischer ◽  
Bernd Weinreich ◽  
Siegfried Nitz ◽  
Friedrich Drawert
Keyword(s):  
Natural Products ◽  
High Speed ◽  
Counter Current

Advances in Separation and Purification of Bioactive Polysaccharides through High-speed Counter-Current Chromatography

2020 ◽  
Vol 58 (10) ◽  
pp. 992-1000
Author(s):  
Yu Yang ◽  
Bilal Muhammad Khan ◽  
Xiping Zhang ◽  
Yongjie Zhao ◽  
Kit-Leong Cheong ◽  
...  
Keyword(s):  
Natural Products ◽  
High Speed ◽  
Solvent System ◽  
Future Research ◽  
Phase System ◽  
Separation And Purification ◽  
Two Phase ◽  
Bioactive Substances ◽  
Efficient System ◽  
Counter Current

Abstract Polysaccharides, with an extensive distribution in natural products, represent a group of natural bioactive substances having widespread applications in health-care food products and as biomaterials. Devising an efficient system for the separation and purification of polysaccharides from natural sources, hence, is of utmost importance in the widespread applicability and feasibility of research for the development of polysaccharide-based products. High-speed counter-current chromatography (HSCCC) is a continuous liquid–liquid partitioning chromatography with the ability to support a high loading amount and crude material treatment. Due to its flexible two-phase solvent system, HSCCC has been successfully used in the separation of many natural products. Based on HSCCC unique advantages over general column chromatography and its enhanced superiority in this regard when coupled to aqueous two-phase system (ATPS), this review summarizes the separation and purification of various bioactive polysaccharides through HSCCC and its coupling to ATPS as an aid in future research in this direction.

Comprehensive separation of secondary metabolites in natural products by high-speed counter-current chromatography using a three-phase solvent system

2007 ◽  
Vol 1151 (1-2) ◽  
pp. 74-81 ◽  
Author(s):  
Akio Yanagida ◽  
Yutaka Yamakawa ◽  
Ryoko Noji ◽  
Ako Oda ◽  
Heisaburo Shindo ◽  
...  
Keyword(s):  
Natural Products ◽  
Secondary Metabolites ◽  
High Speed ◽  
Solvent System ◽  
Three Phase ◽  
Counter Current
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