Validation and Application of Skeletochronology for Age Determination of the Ryukyu Ground Gecko, Goniurosaurus kuroiwae (Squamata: Eublepharidae)

Wound examination is indispensable in forensic practice. The scientific field of wound age determination has advanced progressively during recent years.The purpose of this study was to determine the differences of fibronectin and TGF-β1 expression in both antemortem and postmortem wounds. This study was an experimental with completely randomized design. The skin wounds (vital and postmortem) were taken from fourty Wistar rats and divided into 10 groups of rats. Immunohistochemical staining was performed to determine the differences between antemortem and postmortem wounds. The result showed that in 30 minutes after antemortem wound infliction, all of samples showed weak reactivity for fibronectin and TGF-β1 (100%). In first hour after wound infliction, 3 samples (75%) showed weakly positive and 1 sample (25%) strongly positive for fibronectin and TGF-β1. In 2 hour after wound infliction, 1 sample (25%) showed weakly positive and 3 sample (75%) strongly positive for fibronectin and TGF-β1. In 3 and 4 hour after wound infliction, all of samples strongly positive for fibronectin and TGF-β1. In postmortem wound, all of samples showed negativity for fibronectin and TGF-β1. In conclusion, fibronectin and TGF-β1 may be useful in the determination of wound vitality. Keywords: wound, fibronectin, TGF-β1, vitality

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Problems associated with the age determination of the oldest pottery yielding cultural layers at the Studenoe 1 site, Transbaikal (southern Siberia)

Quaternary International ◽

10.1016/j.quaint.2021.02.002 ◽

2021 ◽

Author(s):

Masami Izuho ◽

Fumie Iizuka ◽

Ian Buvit ◽

Mikhail V. Konstantinov

Keyword(s):

Age Determination ◽

Southern Siberia ◽

Cultural Layers

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Directly ageing the Caribbean spiny lobster, Panulirus argus with validated band counts from gastric mill ossicles

ICES Journal of Marine Science ◽

10.1093/icesjms/fsy177 ◽

2018 ◽

Vol 76 (2) ◽

pp. 442-451 ◽

Cited By ~ 7

Author(s):

Gaya Gnanalingam ◽

Mark J Butler ◽

Thomas R Matthews ◽

Emily Hutchinson ◽

Raouf Kilada

Keyword(s):

Spiny Lobster ◽

Age Determination ◽

Florida Keys ◽

Panulirus Argus ◽

Gastric Mill ◽

Coefficients Of Variation ◽

The Caribbean ◽

First Time ◽

Reader Experience

Abstract In crustaceans, ecdysis was long believed to result in the loss and replacement of all calcified structures, precluding the use of conventional ageing methods. However, the discovery of bands in the gastric ossicles of several crustaceans with some correlation with age suggests that direct age estimation may be possible. We applied this method to a tropical spiny lobster, Panulirus argus, one of the most iconic and economically valuable species in the Caribbean. The presence of growth bands was investigated using wild lobsters of unknown age and was validated with captive reared lobsters of known age (1.5–10 years) from the Florida Keys, Florida (USA). Bands were consistently identified in ptero- and zygo-cardiac ossicles of the gastric mill and did not appear to be associated with moulting. Validation with known age animals confirms that bands form annually. Counts between independent readers were reproducible with coefficients of variation ranging from 11% to 26% depending on reader experience and the structure used. This study demonstrates, for the first time, that direct age determination of P. argus is possible.

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