Analysis of Fibronectin and TGF-β1 Immunoexpression to Determination of Wound Vitality in Wistar Rats (Rattus norvegicus)

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Nita Novita ◽  
Hasrayati Agustina ◽  
Bethy S. Hernowo ◽  
Abdul H. Hassan
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Age Determination ◽  
Scientific Field ◽  
Wound Age ◽  
Forensic Practice ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tgf Β1

Wound examination is indispensable in forensic practice. The scientific field of wound age determination has advanced progressively during recent years.The purpose of this study was to determine the differences of fibronectin and TGF-β1 expression in both antemortem and postmortem wounds. This study was an experimental with completely randomized design.  The skin wounds (vital and postmortem) were taken from fourty Wistar rats and divided into 10 groups of rats. Immunohistochemical staining was performed to determine the differences between antemortem and postmortem wounds. The result showed that in 30 minutes after antemortem wound infliction, all of samples showed weak reactivity for fibronectin and TGF-β1 (100%).  In first hour after wound infliction, 3 samples (75%) showed weakly positive and 1 sample (25%) strongly positive for fibronectin and TGF-β1.  In 2 hour after wound infliction, 1 sample (25%) showed weakly positive and 3 sample (75%) strongly positive for fibronectin and TGF-β1.  In 3 and 4 hour after wound infliction, all of samples strongly positive for fibronectin and TGF-β1.  In postmortem wound, all of samples showed negativity for fibronectin and TGF-β1. In conclusion, fibronectin and TGF-β1 may be useful in the determination of wound vitality. Keywords: wound, fibronectin, TGF-β1, vitality

scholarly journals 31 Determination of Optimal Harvest Weight for Mangalica Pigs Using a Serial Harvest Approach to Measure Growth Performance and Carcass Characteristics

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 34-34
Author(s):  
Maegan A Reeves ◽  
Courtney E Charlton ◽  
Shannon R Wilkerson ◽  
John G Rehm ◽  
Terry D Brandebourg
Keyword(s):  
Live Weight ◽  
Weight Class ◽  
Marbling Score ◽  
Randomized Design ◽  
Carcass Parameters ◽  
Body Weights ◽  
Optimal Harvest ◽  
Completely Randomized Design ◽  
Growth Trial

Abstract Mangalica pigs are a popular niche breed given their reputation for superior quality pork. However, growth and carcass parameters for this breed are poorly documented. Our objective was to better characterize optimal harvest weights for the Mangalica breed. To accomplish this, a growth trial was conducted whereby pigs (n=56) were randomly distributed across stratified harvest weights (50, 57, 68, 82, 93, 102, 127 kg) in a completely randomized design. Pigs were fed standard finisher rations with individual daily feed intakes and weekly body weights recorded for all animals. At 24h postmortem, carcasses were split and ribbed with marbling and loin eye area (LEA) measured at the 10th rib. Primal cuts were fabricated and individually weighed. Fat back was separated from the loin and weighed. As expected, live weight significantly increased across weight class (P < 0.0001). ADG was similar across classes up to 82 kg live weight before steadily declining with increasing weight class (P < 0.0025). Likewise, feed efficiency did not differ between classes until weights heavier than 82 kg (P < 0.03). LEA significantly increased by class up to 82 kg and then plateaued as harvest weight increased further (P < 0.003). Marbling score significantly increased with increasing weight class up to 102 kg where they then plateaued (p < 0.04). Fat back dramatically increased across all weight classes (p < 0.0001) despite negligible increases in LEA or marbling after 102 kg. Primal cut weights for the ham (P < 0.0001), loin (P < 0.0001), Boston butt (P < 0.0001), shoulder (P < 0.0001), and belly (P < 0.0001) all significantly increased with increasing live weight. These data suggest an optimal harvest weight occurs between 82 to 102 kg while offering little objective justification for the current practice of harvesting Mangalica pigs at much heavier live weights.

scholarly journals Reduction in the in vitro sensitivity oF Drechslera tritici-repentis, isolated from wheat, to strobilurin and triazole fungicides

2017 ◽  
Vol 43 (1) ◽  
pp. 20-25
Author(s):  
Rosane Baldiga Tonin ◽  
Erlei Melo Reis ◽  
Aveline Avozani
Keyword(s):  
Inhibitory Concentration ◽  
In Vitro Sensitivity ◽  
Randomized Design ◽  
Inhibition Data ◽  
Ic50 Values ◽  
Control Failure ◽  
Quinone Outside Inhibitors ◽  
Completely Randomized Design

ABSTRACT Reports of failure in the chemical control of wheat yellow leaf spot led to determination of the sensitivity of Drechslera tritici-repentis (Dtr) to the fungicides quinone outside inhibitors (QoIs) and demethylation inhibitors (DMIs). The IC50 was obtained for strobilurins (azoxystrobin, kresoxim-methyl, picoxystrobin and pyraclostrobin) and for triazoles (cyproconazole, epoxiconazole, propiconazole, prothioconazole and tebuconazole), using five Dtr isolates. Seven concentrations of the fungicides were tested in the bioassay: 0.00; 0.01; 0.10; 1.00; 10:00 and 20.00 and 40.00 mg/L active ingredient (a.i.). Assays consisted of completely randomized design and four replicates. Each experiment was performed twice, using the average of the two tests for statistical analysis. The percentage inhibition data for conidial germination (QoIs) and for mycelial growth (DMIs) were subjected to logarithmic regression analysis, calculating the 50% inhibitory concentration (IC50) based on the generated equation. There was a reduction in the sensitivity of Dtr isolates to strobilurins. IC50 values ranged from 0.58 to > 40.00 mg/L. The lowest sensitivity of isolates was detected for azoxystrobin, kresoxim-methyl, picoxystrobin and trifloxystrobin. Pyraclostrobin was most efficient, showing IC50 between 0.58 and 1.03 mg/L. The IC50 ranged from 0.35 to 1.37 mg/L for epoxiconazole, from 0.49 to 1.28 mg/L for propiconazole and from 1.41 to 2.34 mg/L for tebuconazole. Prothioconazole was most potent, showing IC50 between 0.09 and 0.21 mg/L. The hypothesis that the control failure can be attributed to the reduced Dtr sensitivity to the fungicides QoIs and DMIs was confirmed.

scholarly journals Efficiency of portable chlorophyll meters in assessing the nutritional status of wheat plants

2015 ◽  
Vol 19 (12) ◽  
pp. 1148-1151 ◽  
Author(s):  
Alessana F. Schlichting ◽  
Edna M. Bonfim-Silva ◽  
Matheus de C. Silva ◽  
William Pietro-Souza ◽  
Tonny J. A. da Silva ◽  
...  
Keyword(s):  
Nutritional Status ◽  
Chlorophyll Content ◽  
Direct Determination ◽  
Probability Level ◽  
Indirect Determination ◽  
N Concentration ◽  
Randomized Design ◽  
Chlorophyll Index ◽  
Completely Randomized Design

ABSTRACT The objective of this study was to verify the efficiency of two portable chlorophyll meters (Minolta SPAD® 502 and Falker ClorofiLOG® 1030) in assessing the nutritional status of wheat plants, correlating the indices from the devices and the direct determination of chlorophyll content with the concentration of nitrogen (N) in the plant. The experiment was conducted in a greenhouse, in pots with 5 dm3 of Oxisol, in a completely randomized design, with six N doses (0, 80, 160, 240, 320 and 400 mg dm-3) and five replicates. At 47 days after emergence, the readings of SPAD and Falker indices and the quantification of chlorophyll content and N concentration in wheat plants were performed, as well as analysis of variance and correlation test, both at 0.05 probability level. The chlorophyll meters Minolta SPAD® 502 and Falker ClorofiLOG® 1030 do not differ with respect to the indirect determination of chlorophyll in wheat plants. The Falker chlorophyll index was statistically equal to the chlorophyll content. Indirect chlorophyll indices and chlorophyll content showed a high correlation with the N concentration in the plant.

scholarly journals Application of sorting dependent criterias in determination of volume and area bruising of “Golab-Kohanz” apple in Iran

Genetika ◽  
2012 ◽  
Vol 44 (1) ◽  
pp. 177-187 ◽  
Author(s):  
Ahmad Dadashpour
Keyword(s):  
Contact Surface ◽  
Surface Material ◽  
Drop Height ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Damage Criteria ◽  
Apple Fruits ◽  
Area And Volume

Apple fruits are subjected to different loading damage from harvesting to supermarket shelf. Bruising has been attracted many researchers as one of the most important damage criteria. In this research, the effects of some factors such as counter-face material, drop height and linear velocity of apples (conveyor speed) were investigated. Influence of these factors on bruising of ?Golab-Kohanz? variety was analyzed by a Completely Randomized Design (CRD) with factorial test at three levels of drop height (10, 20 and 30 cm), conveyor speed (0.05, 0.1 and 0.15 ms- 1) and four counter-face materials (wood, steel, plastic and cardboard). Tests were conducted at three replications with 108 treatments. Analysis of variance results showed that the effects of drop height and counter-face material on bruising area at 1% level was significant while bruising volume only affected by contact surface material (P<0.01). Mean comparison test indicated that there was no significant difference among levels of conveyor speed on the area and volume bruising. Also, there was no significant difference among levels of drop height on volume bruising while it was significant on the area bruising. Steel and wooden material had no significant effect on the area and volume bruising but their differences with plastic and cardboard were significant. Therefore, drop height and counter-face material must be considered in designing the apple processing systems.

scholarly journals UJI EFEKTIVITAS EKSTRAK ETANOL DAUN PEGAGAN (Centella asiatica (L.) Urban) SEBAGAI ANTIDIABETES TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 736
Author(s):  
Grace Laury Tulung ◽  
Widdhi Bodhi ◽  
Jainer Pasca Siampa
Keyword(s):  
Rattus Norvegicus ◽  
Design Method ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Randomized Design ◽  
Decrease Blood Glucose ◽  
Completely Randomized Design

ABSTRACT Gotu Kola Leaf (Centella asiatica (L.) Urban) are known to contain flavonoid compound, and flavonoid are known to decrease blood glucose level by stimulating pancreas beta cells to produce insulin. The aim of this research is to find out wether the ethanol extract of gotu kola leaf (Centella asiatica (L.) Urban) has the antidiabetic effectiveness or not. This research used Completely Randomized Design method. There were 15 rats used in this research that were split into 5 groups that is negative control (Aquadest), positive control (Metformin), and ethanol extract of gotu kola leaf with the dosage of 5.4 mg;10.8 mg;21.6 mg. The acquired data were analyzed with ANOVA test and LSD test. The analysis result showed that ethanol extract of gotu kola leaf had antidiabetic effectiveness against male white rat. Keywords:  Centella asiatica (L.) Urban, antidiabetic, Rattus norvegicus  ABSTRAK  Daun Pegagan (Centella asiatica (L.) Urban) diketahui mengandung zat flavonoid, dimana flavonoid telah diketahui mempunyai kemampuan menurunkan kadar glukosa darah dengan merangsang sel beta pankreas untuk memproduksi insulin. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun pegagan memiliki efekivitas antidiabetes. Penelitian ini menggunakan metode Rancangan Acak Lengkap. Tikus yang digunakan sebanyak 15 ekor dan dibagi dalam 5 kelompok yaitu kontrol negatif (aquadest), kontrol positif (metformin), dan ekstrak etanol daun pegagan dengan dosis 5,4 mg; 10,8 mg; 21,6 mg. Data yang ada dianalisis dengan menggunakan uji ANOVA dan uji LSD. Hasil analisis menujukkan bahwa ekstrak etanol daun pegagan memiliki efektivitas antidiabetes terhadap tikus putih jantan (Rattus norvegicus). Kata kunci: Centella asiatica (L.) Urban, antidiabetes, Rattus norvegicus

scholarly journals Biokimia Darah Tikus (Rattus norvegicus) Betina Ovariektomi setelah Perlakuan Ekstrak Daun Kaliandra Merah (Calliandra calothyrsus Meissn.)

2019 ◽  
Vol 7 (1) ◽  
pp. 11
Author(s):  
Iriani Setyawati ◽  
Ngurah Intan Wiratmini ◽  
Ni Putu Ayu Dewi Wijayanti
Keyword(s):  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Calliandra Calothyrsus ◽  
Biochemical Tests ◽  
Protein Levels ◽  
Blood Biochemical ◽  
Randomized Design ◽  
Completely Randomized Design

Red calliandra (Calliandra calothyrsus Meissn.) leaf have the potential as phytoestrogens. In this study, adult female ovariectomized rats (as a model of menopause women) were given calliandra leaf extract containing estrogenic flavonoids. This study was an experimental design with a Completely Randomized Design (CRD). Forty-five ovariectomized female rats were divided into five groups i.e. untreated control (K), and the treatment group given 80% of C. calothyrsus ethanolic leaves extract doses 25 mg/ kg bw (P1), 50 mg/ kg bw (P2), 75 mg/ kg bw (P3) and 100 mg/ kg bw (P4). The test suspension was given once a day at 24-hour intervals by gavage as much as 0.5 ml/rat/day for two months. At the end of the treatments, blood was collected for blood biochemical tests including hemoglobin levels, hematocrit values, blood glucose and proteins levels. The results showed that C. calothyrsus leaf extract did not negatively affect the health status of the animals through blood biochemical parameters, i.e. hemoglobin levels, hematocrit values, and glucose and protein levels of ovariectomized female rats.

scholarly journals Comparison of Antibacterial Effectiveness and Length of Healing Between Honey and MEBO in Degree-II Burns at the White Rat (Rattus norvegicus)

2020 ◽  
Vol 13 (1) ◽  
pp. 123-131
Author(s):  
Charles Yapiter ◽  
I Nyoman Ehrich Lister ◽  
Edy Fachrial
Keyword(s):  
Wound Healing ◽  
Experimental Research ◽  
Rattus Norvegicus ◽  
Research Method ◽  
Antibacterial Drug ◽  
White Rats ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Second Degree

Healing Second degree burns can be affected by the presence of bacteria. This research reveals the role of honey and MEBO in removing bacterial effects on healing. Pure experimental research method with a completely randomized design (CRD). There were 4 treatments, which were positive, negative, honey and MEBO treatment, each of which was given to rats with second degree burns. Wound healing and other parameters were observed on 7 th, 14th and 21st days. The result was that the administration of honey as an antibacterial drug was very effective and gave healing on the 21st day to white rats. Giving MEBO as an antibacterial drug is very effective and provides healing on the 21st day to white rats. Giving honey is more effective than MEBO as an antibacterial drug and healing burns on 21st day against white rats.

scholarly journals PENGARUH PENGGUNAAN BUBUK JAHE MERAH (Zingiber officinale var. Rubrum) TERHADAP SIFAT ORGANOLEPTIK BAKSO DAGING KAMBING

ZOOTEC ◽  
2017 ◽  
Vol 38 (1) ◽  
pp. 93
Author(s):  
Afikra Armansyah, ◽  
Friets S. Ratulangi ◽  
Godlief D.G. Rembet
Keyword(s):  
Zingiber Officinale ◽  
Randomized Design ◽  
Ginger Powder ◽  
Completely Randomized Design ◽  
Acceptance Levels ◽  
Materials Used ◽  
Tapioca Flour ◽  
Red Ginger ◽  
Hedonic Quality

THE UTILIZATION OF RED GINGER POWDER (Zingiber officinale var. Rubrum) ON ORGANOLEPTIC CHARACTERISTIC OF MUTTON MEATBALLS. This study was conducted to evaluate the utilization of red ginger powder on the organoleptic characteristic of mutton meatballs. The materials used were fresh mutton meat on the hamstrings, tapioca flour, salt, flavoring, garlic, pepper, red ginger powder, ice cubes and processing equipment tools. The experimental design used in this study was a Completely Randomized Design of five treatments with 30 replications. The treatment was 300 g of meat and 0 g red ginger powder (R0), 300 g of meat and 1.5 g red ginger powder (R1), 300 g of meat and 3.0 g red ginger powder (R2), 300 g of meat and 4.5 g red ginger powder (R3), and 300 g of meat and 6 g red ginger powder (R4). Organoleptic testing performed was using the hedonic quality test. The variables measured consisted of color, flavor, taste and elasticity of meatballs. Determination of the average difference among treatments was done by Tukey (HSD) test. The results showed that the treatments affected significantly on flavor and taste. However, treatments did not affect significantly on color and elasticity of meatballs. The use of 3g red ginger powder could provide acceptance levels on organoleptic characteristic of mutton meatballs. Keywords: Mutton meat, red ginger, Meatballs

scholarly journals Flower microphenology of Hungarian sour cherry cultivars in Iran climatical conditions

2007 ◽  
Vol 13 (3) ◽  
Author(s):  
H. G. Davarynejad ◽  
Z. Szabó ◽  
J. Nyéki ◽  
M. Ansari
Keyword(s):  
Climatic Factors ◽  
Sour Cherry ◽  
Climatic Conditions ◽  
Thermal Variation ◽  
Phenological Stages ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Opening Up

Determination of flower microphenology for selecting the suitable pollinizer for sour cherry cultivars is of significant importance. In order to study the flower microphenology of Hungarian sour cherry (Bőtermő, Érdi jubileum and Cigány) cultivars in Mashhad climatic conditions, an experiment was conducted in I998. 1999, 2005 and 2006, using a completely randomized design with ten replications. The phenological stages were determined from before opening up to browning phase of stigma. Duration of pollen shedding, stigma receptivity and climatic factors were measured. The relative time of flowering between cultivars varied from year to year. The data indicate, the thermal variation strongly contributes to significant differences in duration of stigma viability, although there wasn't a significant difference between cultivars in anther dehiscence period. Phenological stages of flowers are highly affected by meteorological factors in various years.

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