scholarly journals Aqueous and polyphenol-rich Larrea divaricata Cav. extracts as potential skin care agents

2021 ◽  
Vol 4 (2) ◽  
pp. 48-55
Author(s):  
Carla Marrassini ◽  
◽  
Renzo Martino ◽  
Maria Laura Barreiro Arcos ◽  
Elina Malen Saint Martin ◽  
...  
Keyword(s):  
Tritiated Thymidine ◽  
Thymidine Uptake ◽  
Protection Factor ◽  
Trypan Blue Exclusion ◽  
Tritiated Thymidine Uptake ◽  
Uptake Assay ◽  
Larrea Divaricata ◽  
Kinetic Spectrophotometric ◽  
Induced Apoptosis

Skin cells are affected by UV-induced oxidative stress resulting in photoaging and diseases. The objective of this work was to assess the effect of an aqueous extract of Larrea divaricata(Aq) and a flavonoid rich fraction (EA), obtained by liquid/liquid fractionation, on the proliferation of keratinocytes and fibroblasts by the tritiated thymidine uptake assay and on cell viability by the trypan blue exclusion assay. The in vitro sun protection factor (SPF), peroxidase (Px)-like and superoxide dismutase (SOD)-like activities were determined by kinetic spectrophotometric assays, and the phytochemical composition was determined by HPLC-UV and HPLC MS/MS.Aq and EA induced keratinocytes and fibroblast proliferation, protected fibroblasts from H2O2-induced apoptosis and exerted SOD-like and Px-like activities. Aq and EA had a high sun UVB protection factor. So, L. divaricatacould be used in a future for the development of new dermocosmetic or phytotherapy adjuvant in skin oxidative damage.

Damage by Dichlorvos of Human Lymphocyte DNA

1980 ◽  
Vol 66 (4) ◽  
pp. 425-430 ◽  
Author(s):  
Paolo Perocco ◽  
Angela Fini
Keyword(s):  
Dna Synthesis ◽  
Human Lymphocyte ◽  
Tritiated Thymidine ◽  
Human Lymphocytes ◽  
Ultraviolet Rays ◽  
Thymidine Uptake ◽  
Short Term ◽  
In Vitro System ◽  
Tritiated Thymidine Uptake

The action of dichlorvos (2.2-dichlorovinyldimethyl phosphate) was studied with a short-term in vitro system which utilizes human lymphocytes. The parameters studied were the action exerted by the pesticide on scheduled (semiconservative) and unscheduled (reparative) DNA synthesis measured as tritiated thymidine uptake. The results obtained show that dichlorvos affects semiconservative DNA synthesis, damages human lymphocyte DNA inducing low reparative synthesis, and interferes with DNA repair processes after damage exerted by ultraviolet rays.

‘In vivo’ and ‘in vitro’ activity of Larrea divaricata Cav. on EL-4 cells

2010 ◽  
Vol 30 (8) ◽  
pp. 965-971 ◽  
Author(s):  
Roberto Davicino ◽  
Rosario Alonso ◽  
Claudia Anesini
Keyword(s):  
Cell Proliferation ◽  
Aqueous Extract ◽  
Tritiated Thymidine ◽  
Folk Medicine ◽  
Thymidine Uptake ◽  
Stimulatory Action ◽  
T Lymphoma ◽  
Larrea Divaricata

Larrea divaricata is a plant widely used in folk medicine in Argentina. It has been demonstrated that an aqueous extract of L. divaricata possesses a biphasic effect on cell proliferation, at low concentrations exerts a stimulatory action and at high concentrations exerts anti-proliferative effects upon the T lymphoma BW 5147; therefore, we propose in this paper to test the effect of the extract ‘in vitro’ and ‘in vivo’ in another T-cell lymphoma named EL-4. It was analyzed ‘in vitro’ cell proliferation by tritiated thymidine uptake and the effect of the extract on tumors induced in mice analyzing tumor progression and survival.The results showed that the aqueous extract induced the proliferation of tumor cells at all the concentrations studied. The results ‘in vivo’ showed that the aqueous extract stimulated significantly the size of tumors and that untreated mice lived longer than those treated. It is important to be very careful when plant extracts are selected for the treatment of several diseases. Consequently, before using a plant extract, specific scientific studies must be undertaken on different models to certificate therapeutic and adverse effects. Moreover, it can be said that L. divaricata has a specific anti-tumor mechanism of action depending on the targets.

Comparative Sensitivity of Two Mouse Lymphoid Tumour Cell Lines (P388 and P388D1) to Five Anticancer Drugs In Vitro

1990 ◽  
Vol 18 (1_part_1) ◽  
pp. 225-230
Author(s):  
Roland Arnould ◽  
Jacque Dubois ◽  
Fokri Abikhalil ◽  
Anita Libert ◽  
Ghanem Ghanem ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Tritiated Thymidine ◽  
In Vitro Assays ◽  
Lower Sensitivity ◽  
Thymidine Uptake ◽  
Tritiated Thymidine Uptake ◽  
P388 Cell ◽  
Repeated Passage

The sensitivity and the selectivity of two murine lymphoid cell lines (P388 and P388D1) to five chemotherapeutic drugs were investigated in vitro. The cytotoxicities of melphalan, daunorubicin, hexamethylmelamine (HMM), hydroxymethylpentamethylmelamine and dihydroxymethyltetramethylmelamine, two HMM derivatives, were measured in the two cell lines using two different techniques: reduction of a tetrazolium derivative (MTT) and tritiated thymidine uptake into DNA. Cytotoxicity was expressed by the 50% inhibitory concentration (IC50) after one hour and after exposure of cells to each drug for two days. The IC50 results indicate that the P388 cells were generally more sensitive to melphalan, daunorubicin, hydroxymethylpentamethylmelamine and dihydroxymethyltetramethylmelamine than the P388D1 cell line. HMM was found to be inactive in both cell lines. Despite the lower sensitivity of the P388D1 cell line compared with the P388 cell line, because of its greater homogeneity, it could replace the P388 line for the in vitro assays, bearing in mind that the P388D1 cell line sensitivity is 1.21 - 24.63 times lower than that of the P388 cell line, at least as far as the drugs tested are concerned. Moreover, our results emphasise that variations of sensitivity could occur with repeated passage in vitro.

Toxic, DNA-Damaging and Mutagenic Activity of Epichlorohydrin on Human Cells Cultured in Vitro

1983 ◽  
Vol 69 (3) ◽  
pp. 191-194 ◽  
Author(s):  
Paolo Perocco ◽  
Paola Rocchi ◽  
Anna Maria Ferreri ◽  
Antonella Capucci
Keyword(s):  
Cell Viability ◽  
Diphtheria Toxin ◽  
Mutagenic Activity ◽  
Tritiated Thymidine ◽  
Human Lymphocytes ◽  
Metabolic Activation ◽  
Thymidine Uptake ◽  
Tritiated Thymidine Uptake ◽  
Cells Cultured

Epichlorohydrin (ECHH) highly inhibited the tritiated thymidine uptake by human lymphocytes cultured in vitro, although the corresponding cell viability was unaffected. Furthermore, it elicited unscheduled DNA synthesis, acting as a DNA-damaging agent after its metabolic activation. ECHH also showed a clear toxic and mutagenic activity toward a human epithelial-like cell line, causing a decrease in cell viability and an increase in mutants resistant to 0.05 Lf/ml of diphtheria toxin.

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